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TCGA whole-transcriptome sequencing data reveals significantly dysregulated genes and signaling pathways in hepatocellular carcinoma

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Frontiers of Medicine 2015年第3期9卷 322-330页

作者： Daniel Wai-Hung ho Alan Ka-Lun Kai Irene Oi-Lin ng Department of Pathology and State Key Laboratory for Liver Research The University of Hong Kong ttong Kong SAR China

This study systematically evaluates the TCGA whole-transcriptome sequencing data of hepatocellular carcinoma （HCC） by comparing the global gene expression profiles between tumors and their corresponding non- tumorous liver tissue. Based on the differential gene expression analysis, we identified a number of novel dysregulated genes, in addition to those previously reported. Top-listing upregulated （CENPF and FOXM1） and downregulated （CLEC4G, CRHBP, and CLEC1B） genes were successfully validated using qPCR on our cohort of 65 pairs of human HCCs. Further examination for the mechanistic overview by subjecting significantly upregulated and downregulated genes to gene set enrichment analysis showed that different cellular pathways were involved. This study provides useful information on the transcriptomic landscape and molecular mechanism of hepatocarcinogenesis for development of new biomarkers and further in-depth characterization.

关键词： TCGA whole-transcriptome sequencing HCC liver cancer

Viral integration detection strategies and a technical update on Virus-Clip

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BIOCELL 2021年第6期45卷 1495-1500页

作者： DANIEL WAI-HUng ho XUEYIng LYU IRENE OI-LIN ng Department of Pathology The University of Hong KongHong KongChina State Key Laboratory of Liver Research The University of Hong KongHong KongChina

Oncovirus infection is crucial in human *** oncoviruses can lead to structural variations in the human genome known as viral genomic integration,which can contribute to *** viral integration detection tools differ in their underlying algorithms pinpointing different aspects or features of viral integration *** discuss about major procedures in performing viral integration *** importantly,we provide a technical update on Virus-Clip to facilitate its usage on the latest human genome builds(hg19 and hg38)and the adoption of multi-thread mode for faster initial read *** comparing the execution of Virus-Clip using single-thread and multi-thread modes of read alignment on targeted-panel sequencing data of HBV-associated hepatocellular carcinoma patients,we demonstrate the marked improvement of multi-thread mode in terms of significantly reduced execution time,while there is negligible difference in memory *** together,with the current update of Virus-Clip,it will continue supporting the in silico detection of oncoviral integration for better understanding of various human malignancies.

关键词： Oncovirus Viral genomic integration In silico detection Tumorigenesis Human malignancies

Photocatalytic Bacterial Disinfection in a Partition System

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Photocatalytic Bacterial Disinfection in a Partition System

Single cell analysis informing therapy response in hepatocellular carcinoma and intrahepatic cholangiocarcinoma

2012年环境污染与大众健康学术会议

作者： Tsz Wai ng Po keung WOng Wing Kei ho School of Life Sciences The Chinese University of hong Kong Shatin hong Kong SAR China

ISBN: (纸本)9781618960108

Photocatalytic disinfection has been well-studied for water treatment. however, the post-separation of photocatalyst remains a major challenge for practical application. This study developed a novel method for photocatalytic disinfection, which used a "partition system" to retain the photocatalyst. Cadmium indium sulfate （CdIn 2 S 4 ） coupled with regenerated cellulose membrane gave the best performance. 4 log-reduction of a Gram-negative bacterium, Escherichia coli, and 5 log-reduction of a Gram-positive bacterium, Microbacterium Barkeri, （initial cell density 2×10 5 cells/mL） were achieved within 4 and 6 h, respectively. Scavenger study revealed that H 2 O 2 is the major reactive species for bacterial inactivation in the "partition system". The study provides an alternative cost-effective method for the application of photocatalysis in water disinfection.

关键词： Photocatalysis Bacterial disinfection Partition system

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Hepatobiliary Surgery and Nutrition 2022年第1期11卷 126-128页

作者： Daniel Wai-Hung ho Irene Oi-Lin ng Department of Pathology The University of Hong KongHong KongChina State Key Laboratory of Liver Research The University of Hong KongHong KongChina

Liver cancer is known to have significant degree of intra-tumoral heterogeneity(ITH)(1).Hepatocellular carcinoma(HCC)and intrahepatic cholangiocarcinoma(ICC)are major forms of primary liver cancer,accounting for>95%of *** is linked,as least in part,to the aggressiveness and refractoriness of liver cancer,rendering its high lethality among human malignancies(2).The complex tumor microenvironment promotes tumor progression and contributes to poor treatment ***,it is vital to address the biological mechanisms underlying liver cancer progression and treatment response analyzing the cells in high resolution rather than the mixture of many cell *** advances in single-cell RNA sequencing(scRNA-seq)provide an avenue to dissect liver cancer in an unprecedented degree of high resolution to characterize the underlying pathogenesis and heterogeneity.

关键词： cancer intrahepatic Liver

髓母细胞瘤与幕上原始神经外胚叶肿瘤中RASSF1A基因的甲基化差异

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中华病理学杂志 2007年第1期36卷 24-28页

作者：常青吴浩强北京大学医学部病理系 100083 香港中文大学威尔斯亲王医院病理解剖及细胞学系

目的通过研究髓母细胞瘤与幕上原始神经外胚叶肿瘤(SPNET)中 RASSF1A 基因的甲基化改变,探讨颅内原始神经外胚叶肿瘤(PNET)的不同亚型中该基因的表遗传学差异及其意义。方法收集25例原发髓母细胞瘤,9例原发 SPNET,3株髓母细胞瘤细胞系和... 详细信息

目的通过研究髓母细胞瘤与幕上原始神经外胚叶肿瘤(SPNET)中 RASSF1A 基因的甲基化改变,探讨颅内原始神经外胚叶肿瘤(PNET)的不同亚型中该基因的表遗传学差异及其意义。方法收集25例原发髓母细胞瘤,9例原发 SPNET,3株髓母细胞瘤细胞系和2株 SPNET 细胞系。采用甲基化特异性聚合酶链反应(MSP)检测 RASSF1A 基因启动子区的甲基化状态。应用去甲基化试剂5-aza-2′deoxycytidine 处理存在基因表达缺失的细胞系,探讨基因表达与甲基化之间的关系。结果100%(25/25)的原发髓母细胞瘤、6/9的原发 SPNET 及全部 PNET 细胞系中均检测到RASSFIA 基因的甲基化。相反,该基因甲基化在全部正常组织(包括2例小脑,5例大脑)中均未检测到。并且,RASSF1A 在 SPNET 中的甲基化率明显低于髓母细胞瘤(Fisher 精确检验,P=0.014)。在经去甲基化试剂处理的 PNET 细胞中,该基因表达得以恢复,证明甲基化与该基因沉默相关。结论RASSF1A 甲基化是肿瘤特异性的,RASSF1A 甲基化与 PNET 的发生有一定关联,不同亚型的 PNET 之间 RASSF1A 基因的不同甲基化状态提示髓母细胞瘤和 SPNET 是表遗传学上存在差异的两类肿瘤。

关键词：髓母细胞瘤神经外胚瘤原始 DNA甲基化 RASSF1A

Agglutination of Helicobacter pylori coccoids by lectins

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World Journal of Gastroenterology 2000年第2期6卷 202-209页

作者： Mar Mar Khin Jie Song Hua Han Cong ng Torkel Wadstrm Bow ho Departments of Microbiology Faculty of MedicineNational University of Singapore5Science Drive 2Singapore 117597Republic of Singapore Department of Medical Microbiology Lund UniversitySolvegatan 23S 223 62 LundSweden

AIM To study the agglutination pattern ofHelicobacter pylori coccoid and spiral *** Assays of agglutination andagglutination inhibition were applied usingfifteen commercial *** Strong agglutination was observedwith mannose-specific Concanavalin A(Con A),fucose-specific Tetragonolobus purpureas(Lotus A)and N-acetyl glucosamine-specificTriticum vulgaris(WGA)*** andfucose specific lectins were reactive with allstrains of *** coccoids as compared to *** carbohydrates,glycoproteinsand mucin were shown to inhibit ***-agglutination ***-treatment ofthe bacterial cells with formalin and sulphuricacid did not alter the agglutination patterns ***,sodium periodate treatment ofbacterial cells were shown to inhibitagglutination reaction with Con A,Lotus A andWGA *** the contrary,enzymatictreatment of coccoids and spirals did not showmarked inhibition of ***,heating of *** cells at 60℃ for 1 hour was shown toaugment the agglutination with all of the *** The considerable differences inlectin agglutination patterns seen among the twodifferentiated forms of *** might beattributable to the structural changes during theevents of morphological transformation,resulting in exposing or masking some of the sugar residues on the cell *** ofvarious sugar residues on the cell wall of thecoccoids may allow them to bind to differentcarbohydrate receptors on gastric mucus andepithelial *** coccoids with adherencecharacteristics like the spirals could aid in thepathogenic process of Helicobacter *** may probably lead to different clinicaloutcome of *** associated gastroduodenaldisease.

关键词： Helicobacter pylori coccoids lectin gastric mucosa

Syntheses,structures,and reactivity of ruthenium(Ⅱ) hydride complexes containing Klui's oxygen tripodal ligand

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Science China Chemistry 2010年第10期53卷 2139-2143页

作者： ng ho-Yuen LAI Chun-Sing WILLIAMS Ian D. LEUng Wa-Hung Department of Chemistry The Hong Kong University of Science and Technology

Treatment of Ru(CO)(Cl)(H)(PPh3)3 with NaLOEt (LOEt-= [CpCo{P(O)(OEt)2}3]-) afforded the hydride complex (PPh3)(CO)-LOEtRu(H) (1), which has been characterized by X-ray crystallography. Similarly, the tricyclohexylphosphine analogue, (PCy3)(CO)LOEtRu(H) (2), was synthesized from Ru(CO)Cl(H)(PCy3)2 and NaLOEt. Treatment of complex 1 with R'SO2N3 afforded the (arylsulfonyl)amido complexes LOEtRu(CO)(PPh3)(NHSO2R) (R = 2,4,6-i-Pr3C6H2 (3), 4-t-BuC6H4 (4)). The crystal structure of complex 3 has been determined. The Ru-N distance and Ru-N-S angle in 3 are 2.076(3) and 126.14(16)°, respectively. Reactions of complex 1 with acids have been studied.

关键词： ruthenium oxygen tripodal ligand hydride complex (arylsulfonyl)amido complex

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脑节细胞性胶质瘤的分子遗传学研究

中华病理学杂志 2005年第3期34卷 147-149页

作者：殷晓璐吴浩强第二医科大学附属仁济医院病理科上海200127 香港中文大学威尔斯亲王医院病理解剖及细胞学系

目的通过研究脑节细胞性胶质瘤全基因组的遗传学改变,探讨该肿瘤的发病机制。方法应用比较基因组杂交技术,分析脑节细胞性胶质瘤全基因组的遗传学改变。结果收集脑节细胞性胶质瘤5例,男性3例,女性2例。其中,3例肿瘤发现有9号染色体短臂(... 详细信息

目的通过研究脑节细胞性胶质瘤全基因组的遗传学改变,探讨该肿瘤的发病机制。方法应用比较基因组杂交技术,分析脑节细胞性胶质瘤全基因组的遗传学改变。结果收集脑节细胞性胶质瘤5例,男性3例,女性2例。其中,3例肿瘤发现有9号染色体短臂(9p)的丢失,2例有7号染色体的获得,该结果通过荧光原位杂交(FISH)技术得到了进一步的证实。应用免疫组织化学(ABC法)染色,位于染色体7p11-p13上的癌基因表皮生长因子受体(EGFR)在所有5例节细胞性胶质瘤中都无异常表达。此外,在染色体2q33-q34,8q12-q22,14q21-qter,15q-26qter和Y上也都发现了遗传物质的丢失或扩增。结论染色体9p的丢失及7号染色体的获得可能与脑节细胞性胶质瘤的发病机制有关。

关键词：胶质瘤节细胞肿瘤遗传学改变 9号染色体分子遗传学发病机制全基因组遗传物质发现

The refined physical parameters of transiting exoplanet system HAT-P-24

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Research in Astronomy and Astrophysics 2013年第5期13卷 593-603页

作者： Xiao-Bin Wang Sheng-hong Gu Andrew Collier Cameron hong-Bo Tan ho-keung Hui Chi-Tai Kwok Bill Yeung Kam-Cheung National Astronomical Observatories/Yunnan Observatory Chinese Academy of Sciences Key Laboratory for the Structure and Evolution of Celestial Objects Chinese Academy of Sciences School of Physics and Astronomy University of St Andrews North Haugh St Andrews Fife KY16 9SS UK University of Chinese Academy of Sciences hokoon Nature Education cum Astronomical Centre Sik Sik Yuen hong Kong Astronomical Society

The transiting exoplanet system HAT-P-24 was observed by using CCD cameras at Yunnan Observatory and hokoon Astronomical Centre, China in 2010 and 2012. In order to enhance the signal to noise ratio of transit events, the observed data are corrected for systematic errors according to Collier Cameron et al.＇s coarse de- correlation and Tamuz et al.＇s SYSREM algorithms. Three new complete transit light curves are analyzed by means of the Markov chain Monte Carlo technique, and the new physical parameters of the system are derived. They are consistent with the old ones from the discovered paper except for a new larger radius Rp = 1.364 Rj of HAT- P-24b, which confirms its inflated nature. By combining the five available epochs of mid-transit derived from complete transit light curves, the orbital period of HAT-P- 24b is refined to P = 3.3552479 d and no obvious transit timing variation signal can be found from these five transit events during 2010-2012.

关键词： exoplanet system -- transit -- individual: HAT-P-24