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Rbfox1 controls alternative splicing of focal adhesion genes in cardiac muscle cells

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Journal of Molecular Cell Biology 2024年第1期16卷 54-65页

作者： Peter Zorn Jaime Calvo Sánchez Tala Alakhras Barbara Schreier Michael Gekle Stefan Hüttelmaier Marcel Köhn Junior Group‘Non-coding RNAs and RBPs in Human Diseases’ Medical FacultyUniversity of Halle–Wittenberg06120 Halle(Saale)Germany Julius Bernstein Institute of Physiology Medical FacultyUniversity of Halle–Wittenberg06112 Halle(Saale)Germany Institute of Molecular Medicine Medical FacultyUniversity of Halle–Wittenberg06120 Halle(Saale)Germany

Alternative splicing is one of the major cellular processes that determine the tissue-specific expression of protein ***,it remains challenging to identify physiologically relevant and tissue-selective proteins that are generated by alternative ***,we investigated the target spectrum of the splicing factor Rbfox1 in the cardiac muscle context in more *** using a combination of in silico target prediction and in-cell validation,we identified several focal adhesion proteins as alternative splicing targets of *** focused on the alternative splicing patterns of vinculin(metavinculin isoform)and paxillin(extended paxillin isoform)and identified both as potential Rbfox1 *** analyses suggested that both isoforms are promoted by Rbfox1 due to binding in the *** adhesions play an important role in the cardiac muscle context,since they mainly influence cell shape,cytoskeletal organization,and cell–matrix *** data confirmed that depletion of Rbfox1 changed cardiomyoblast morphology,cytoskeletal organization,and multinuclearity after differentiation,which might be due to changes in alternative splicing of focal adhesion ***,our results indicate that Rbfox1 promotes alternative splicing of focal adhesion genes in cardiac muscle cells,which might contribute to heart disease progression,where downregulation of Rbfox1 is frequently observed.

关键词： Rbfox1 alternative splicing focal adhesion cardiac muscle cells Topic:cardiac myocytesalternative splicingfocal adhesionsgenesintronsprotein isoformsrna splicing

Customized molecular tools to strengthen metabolic engineering of cyanobacteria

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Green Carbon 2024年第2期2卷 149-163页

作者： Stephan Klähn Franz Opel Wolfgang R.Hess Department of Solar Materials Biotechnology Helmholtz Centre for Environmental Research–UFZLeipzig 04318Germany Genetics and Experimental Bioinformatics Albert-Ludwig University FreiburgFreiburg im Breisgau 79104Germany

Cyanobacteria are promising oxygenic phototrophs for the production of various *** their(photo)biotechnological exploitation,molecular tools are required,such as,for the introduction and expression of heterologous genes,or the modulation of enzyme activities or entire *** and strategies for the development of photosynthetic biomanufacturing technologies based on cyanobacteria have been extensively reviewed,as well as certain specialized aspects of their genetic ***,options for metabolic engineering of specific cyanobacterial cells are still less developed than those for other bacteria of biotechnological *** addition to the standard genetic toolbox for“classical”metabolic engineering,we emphasize certain aspects,including recently developed vector systems for the extrachromosomal maintenance of genes and approaches based on clustered regularly interspaced short palindromic repeats(CRISPR)*** highlight the development of custom molecular tools for specific strains or products,discuss the emerging use of small regulatory proteins that appear promising for advanced metabolic engineering approaches to promote specific product formation,and provide an overview of suitable online ***,we discuss the current trends in this field and indicate their potential,such as using suitable product sensors that enable systematic screening,and optimization approaches.

关键词： Metabolic engineering Cyanobacteria Synthetic plasmids Regulatory proteins Sensors

Bio-inspired tetracarbene compounds as a new family of energy saving catalysts

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Journal of Energy Chemistry 2023年第4期79卷 559-561页

作者： Bo Zhang Fritz E.Kühn CAS Key Laboratory of Science and Technology on Applied Catalysis Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023LiaoningChina' University of Chinese Academy of Science Beijing 100049China Molecular Catalysis Catalysis Research Center and Department of ChemistrySchool of Natural SciencesTechnical University of MunichLichtenbergstr 485748 Garching bei MünchenGermany

Efficient use of energy is a pressing *** of chemical processes is currently an important target for energy *** chemical processes are independent of pathways with respect to the final energy output or consumption,the application of catalysts can reduce involved activation energies considerably and therefore save large amounts of energy.

关键词： Catalysis Iron Carbenes Multidentate ligands

Strong coupling,charm- and bottom-quark masses from electron-positron annihilation

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Chinese Physics C 2010年第6期34卷 675-679页

作者： J.H.Kühn Institut für Theoretische Teilchenphysik Karlsruhe Institute of Technology

The cross section for electron-positron annihilation into hadrons allows for a precise determination of the strong coupling constant and the charm- and bottom-quark masses. Recent theoretical and experimental results ... 详细信息

关键词： strong coupling quark masses

Seismic Response Reduction for Fixed Offshore Platform by Tuned Liquid Damper

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China Ocean Engineering 1997年第2期12卷 119-125页

作者： Li, hn Ma, BC Shenyang Architectural and Civil Engineering Institute Shenyang 110015 China

An approach to seismic response reduction for offshore platforms by the use of the tuned liquid damper is presented in this paper. First of all, the effects of the tuned frequency ratio and excited frequency ratio on the seismic response reduction of the platform structure are investigated. Based on the results, a mechanical model and equation of motion for the TLD-platform system are established. And then effectiveness of the appraoch is verified by numerical calculation.

关键词： fixed offshore platform tuned liquid damper vibration reduction

A NEW RAPID FLUOROMETRIC METHOD FOR DETERMINATION OF SULFONAMIDES

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Chinese Chemical Letters 1995年第6期6卷 495-496页

作者： Li, hn Ci, YX Peking Univ Dept Chem Beijing 100871 Peoples R China

A new fluorometric method for the determination of sulfonamides is described, based on the formation of a fluorescent inclusion complex of carbonic anhydrase(CA) with dansylamide (DNSA). The fluorescence maximum emission of the complex is at 460nm with excitation at 280nm. Dissociation constants were determined for the CA-sulfonamide complexes. Limits of detection for DNSA,sulfanilamide(SAN) and p-toluenesulfonamide (PTSN) were 0.84, 19.5 and 6.1 ng/ml, respectively. The recommended method, was applied. to the determination of sulfonamides in cow milk at ng/ml level.

关键词： RAPID

Organorhenium(Ⅶ) oxides

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Rare Metals 2006年第Z1期25卷 411-421页

作者： Kühn Fritz E. JAIN Kavita R. Department für Chemie der Technischen Universitt München Lichtenbergstrasse 4 D-85747 Garching bei München Germany

Methyltrioxorhenium (MTO) and its derivatives were utilized as catalysts for a broad variety of organic reactions after the discovery of an easy synthetic access way to this class of compounds. Among the numerous characterization techniques, 17O-NMR spectroscopy and vibrational spectroscopy were exemplary discussed. The immobilization of MTO and its derivatives is of significant current interest as is the synthesis of chiral derivatives.

关键词： catalysis epoxidation immobilization olefination rhenium

In vitro bioactivity and phytochemical screening of Suaeda maritima(Dumort):A mangrove associate from Bhitarkanika,India

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Asian Pacific Journal of Tropical Medicine 2011年第9期4卷 727-734页

作者： JK Patra NK Dhal hn Thatoi Department of Biotechnology North Orissa UniversityBaripada-757003OdishaIndia Department of Natural Products Institute of Minerals and Materials TechnologyBhubaneswar-751013OdishaIndia Department of Biotechnology College of Engineering and Technology(Bijn Patnaik University of Technology)Bhubaneswar-751003OdishaIndia

Objective:To investigate the in vitro antioxidant and antimicrobial activities along with phytochemical screening of organic and aqueous extracts of leaf and stem of Suaeda maritima (Dumort).a mangrove associate from Bhitarkanika of ***:Antioxidant activity of the crode extracts was evaluated in terms of total antioxidant capacity,total phenol content,ascorbic acid content,DPPH radical scavenging,metal chelating,nitric oxide scavenging,and reducing power *** antimicrobial activity of the plant was determined by agar well diffusion method along with MIC and BBC carried out by microdilulion techniques against 10 gram positive and gram negative human pathogenic *** qualitative and qualitative phytochemical screening were carried out by standard biochemical ***: Out of the seven antioxidant bioassavs,both the leaf and stem extracts were found to posses strong antioxidant properties of 70%to 92%for phenol,total antioxidant capacity,DPPH free radical scavenging aclivitv and fairly good ascorbic acid content,metal chelating(1.33%-22.55%). reducing power(0.01-0.12) and nitric oxide scavenging(0.84%-66.99%) *** of the four extracts evaluated for antimicrobial activity,two leaf extracts such as acetone and ethanol showed promising activity against four pathogenic bacteria and one stem methanol extracts against one pathogenic bacteria when compared with amoxcycillin as *** MIC and MBC values of the antimicrobial extracts ranged between 2.5 to 5.0 mg/*** of phytochemicals showed presence of carbohydrates,protein,tannins,alkaloids and flavonoids in comparatively higher amount than other phytochemicals ***:The present study reveals the presence of potential antioxidants and antimicrobial properties in the plant extract which could be exploited for pharmaceutical application.

关键词： Mangrove plants Suaeda maritima Antioxidant Antimicrobial Phytochemicals

Accelerating the Feedback Loop in Scanning Probe Microscopes without Loss of Height Measurement Accuracy by Using Pixel Forecast Methods

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Journal of Applied Mathematics and Physics 2022年第4期10卷 1325-1334页

作者： Robert Kö hn Boris Braun Kai-Felix Braun Engineering Department Wilhelm Büchner University of Applied Sciences Darmstadt Germany

Scanning probe microscopes (SPM) are limited in their speed of data acquisition by the mechanical stability of the scanner. Therefore many types of scanners have been developed to achieve a rigid setup while maintaining an acceptable image size. We have followed here a different path to accelerate data acquisition by improving the feedback loop to achieve the same SPM image quality in a shorter time. While the feedback loop in a scanning probe microscope typically starts to probe a new pixel starting from the previous position, we have reduced the total control time by using an improved starting point for the feedback loop at each pixel. By exploiting the information of the already scanned pixels a forecast for the new pixel is created. We have successfully used several simple methods for a prognosis in MATLAB simulations like one dimensional linear or cubic extrapolation and others. Only scanning tunnelling microscope data from real experiments were used to test the forecasts. A doubling of the speed was achieved in the most favourable cases.

关键词： Scanning Probe Microscopy Feedback Loop Controller Simulation