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Education Programs for Invasive Procedures Involving Nurses: A Scoping Review

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Open Journal of Nursing 2024年第5期14卷 200-224页

作者： Hiromi Shibuya Akiko Saito Masumi Mugiyama Noyuri Yamaji Chisato eto Satoshi Shibuya Department of Nursing Faculty of Health Science Technology Bunkyo Gakuin University Tokyo Japan Department of Health Care Policy and Health Economics Institute of Medicine University of Tsukuba Ibaraki Japan Institute of Clinical Epidemiology Showa University Tokyo Japan Department of Family Nursing Division of Health Science and Nursing Graduate School of Medicine The University of Tokyo Tokyo Japan Department of Global Health Nursing Graduate School of Nursing St. Luke&#8217s International University Tokyo Japan School of Nursing Tokyo Women&#8217s Medical University Tokyo Japan Department of Integrative Physiology School of Medicine Kyorin University Tokyo Japan

Background: Implementing invasive procedures is an important part of patient management by clinical nurses;however, there are gaps between nurses’ actual knowledge and skills and expected professional care capacity. Purpose: This scoping review aimed to map the existing literature related to recent institution-provided educational programs for invasive procedures involving clinical nurses. This study seeks to understand the contents of educational programs and the methods for assessing educational effects. Methods: This scoping review was completed using the following four databases: PubMed (MEDLINE), Embase, Cochrane Library, and Emcare for the period 2000-2022. We included studies that used all forms of educational approaches (e.g., didactic lectures, hands-on training, or on-the-job training). This scoping review considered peer-reviewed publications published in English using quantitative, qualitative, or mixed approaches. A total of 83 studies underwent in the final analysis. Results and Conclusion: A combination of didactic lectures and hands-on training was provided as an educational program in most studies. Contrary to our prediction, educational interventions with advanced technologies such as VR are extremely rare, suggesting that the effectiveness of advanced technologies in learning invasive procedures should be examined to facilitate and retain educational effects more efficiently in future studies. Regarding the assessment of educational effects, nurses’ cognitive (i.e., theoretical knowledge about procedures), psychomotor (i.e., implementing procedures), and psychological aspects (e.g., confidence and self-efficacy in procedures) were evaluated using questionnaires and observational methods. While most studies used a one-group pretest-posttest design, the ratio of randomized controlled trials (RCT) was relatively low. Thus, an RCT design should be introduced in future studies to test the validity of the developed educational program more accurately.

关键词： Training Education Invasive Procedure Nurses Assessment

非血管化的脂肪移植后脂肪细胞的命运：脂肪细胞早期死亡和替换的证据

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中国美容整形外科杂志 2013年第11期24卷 I0005-I0013页

作者： hitomi eto Harunosuke Kato Hirotaka Suga Noriyuki Aoi Kentaro Doi Shinichiro Kuno Kotaro Yoshimura 魏峰(译者) 高景恒(译者) 袁继龙(译者) 东京大学医学院整形外科日本东京《中国美容整形外科杂志》编辑部辽宁省人民医院整形外科

背景与目的脂肪移植的临床效果是一种可变因素，而且其依赖于所采用的技术，同时，移植组织怎样建立血管化是未知的。近来，我们通过对蛋白进行免疫组化染色，而非HE染色，对存活和死亡的脂肪细胞进行观察。方法利用如下方法对人类脂肪... 详细信息

背景与目的脂肪移植的临床效果是一种可变因素，而且其依赖于所采用的技术，同时，移植组织怎样建立血管化是未知的。近来，我们通过对蛋白进行免疫组化染色，而非HE染色，对存活和死亡的脂肪细胞进行观察。方法利用如下方法对人类脂肪组织中各细胞成分（脂肪细胞，脂肪干细胞／间质细胞／祖细胞，血管内皮细胞，造血细胞）的生存能力进行评价：①储存脂肪抽吸物；②培养细胞；③培养功能性脂肪组织。此外，将小鼠腹股沟脂肪垫（150～200mg）移植于头皮下，并在第0、1、2-,3、5、7、14天对移植物进行染色。结果体外研究显示，尽管脂肪来源间质干细胞可以保持3d的活性，但在缺血环境条件下脂肪细胞最易受影响而死亡。体内研究证实，大多数移植脂肪细胞在第1天开始死亡，仅有少部分距离组织边缘300μm的脂肪细胞存活。增殖细胞的数量在第3天开始增多，从第7天开始活性脂肪细胞区域增大，提示坏死组织的修复重建过程开始。结论我们展示了非血管化的脂肪移植后脂肪组织最大程度有活力模型的令人信服的证据。我们观察到移植组织由外围至内部分为3个区域：存活区域（脂肪细胞存活），再生区域（脂肪细胞死亡，脂肪来源间质干细胞存活，坏死脂肪细胞由新生的脂肪细胞替换），坏死区域（脂肪细胞核脂肪来源间质干细胞均死亡）。

关键词：脂肪细胞早期死亡非血管化移植后证据免疫组化染色细胞存活间质干细胞

抽吸与切取方法获取的脂肪组织的结构特征和细胞组成的对比研究

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中国美容整形外科杂志 2011年第7期22卷 I0022-I0022页

作者： hitomi eto Hirotaka Suga Daisuke Matsumoto Keita Inoue Noriyuki Aoi Harunosuke Kato Jun Araki Kotaro Yoshimura 魏峰(译) 高景恒(译) 张晨(译) 日本东京东京大学医学院整形外科《中国美容整形外科杂志》编辑部大连大学附属新华医院整形外科

背景脂肪组织是一种容易获取的组织，可当做软组织填充物，也是成人多功能细胞的来源，这种多功能细胞被称为脂肪源性干细胞／基质细胞／祖细胞。然而，由于脂肪细胞和脂肪组织的结构很脆弱，脂肪细胞的许多解剖特征仍不清楚。方法纳入... 详细信息

背景脂肪组织是一种容易获取的组织，可当做软组织填充物，也是成人多功能细胞的来源，这种多功能细胞被称为脂肪源性干细胞／基质细胞／祖细胞。然而，由于脂肪细胞和脂肪组织的结构很脆弱，脂肪细胞的许多解剖特征仍不清楚。方法纳入抽吸方法获取的皮下脂肪组织标本15例，完整皮下脂肪组织标本9例，采用以下方法进行评估：①采用组织全层包埋结合三重荧光染色技术，对活脂肪组织进行三维成像；②甘油一3．磷酸脱氢酶试验；③多色流式细胞术（CD34、CD31和CD45）；④培养血管基质部分细胞的贴壁细胞，以产生脂肪源性基质细胞。结果组织全层包埋方法显示脂肪组织旁存在毛细血管网，抽吸获取的脂肪组织中的毛细血管网被部分破坏。抽吸脂肪组织也缺少大的脉管结构，其小脂肪粒（破碎脂肪组织，P=0．00016）和死亡细胞（P=0．0038）的数量均高于切取脂肪组织。抽吸与切取的脂肪组织中，脂肪细胞数量占全部细胞数的比例均小于20％，且脉管系统相关细胞（包括内皮细胞和脂肪源性基质细胞）占到细胞总量的一半以上。甘油一3．磷酸脱氢酶试验显示，抽吸和切取脂肪组织中破裂的脂肪组织所占百分比分别为大于30％和5％（P=0．032）。多色流失细胞术分析结果显示，抽吸脂肪组织中受污染的血源性细胞（CD45＋）数量在总细胞中所占百分比更大（P=0．0038），抽吸脂肪组织内的脂肪源性基质细胞产量大约是切取脂肪组织的一半（P=0．011）。结论作者研究表明两种组织有不同的结构特征和细胞成分，且抽吸脂肪组织内组织损伤更严重，祖细胞产量也不足。

关键词：脂肪组织细胞组成抽吸方法切取方法结构特征脂肪源性干细胞毛细血管网多色流式细胞术

局部缺血时的脂肪组织重构：脂肪细胞的死亡与干／祖细胞的激活

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中国美容整形外科杂志 2011年第7期22卷 I0023-I0023页

作者： Hirotaka Suga hitomi eto Noriyuki Aoi Harunosuke Kato Jun Araki Kentaro Doi Takuya Higashino Kotaro Yoshimura 魏峰(译) 高景恒(译) 张晨(译) 日本东京东京大学医学院整形外科《中国美容整形外科杂志》编辑部大连大学附属新华医院整形外科

背景许多整形外科手术后（如脂肪移植），脂肪组织开始缺血，导致组织缺氧和营养缺失。然而，很少有研究检测脂肪组织中的缺血和／或缺氧变化的报道。方法切断小鼠体内为腹股沟脂肪垫供血的血管，建立外科手术诱导的缺血模型。利用氧气... 详细信息

背景许多整形外科手术后（如脂肪移植），脂肪组织开始缺血，导致组织缺氧和营养缺失。然而，很少有研究检测脂肪组织中的缺血和／或缺氧变化的报道。方法切断小鼠体内为腹股沟脂肪垫供血的血管，建立外科手术诱导的缺血模型。利用氧气监视器测量脂肪组织的氧分压，利用全标本染色、免疫组化、流式细胞术和蛋白质印迹法分析缺血改变。作者还检测了体外低氧环境下的细胞寿命。结果3种程度缺血模型（轻度、中度、重度）的氧分压水平分别为正常脂肪组织（50．5±1．3）mmHg的75％、55％、20％。在第28天观察到脂肪组织萎缩伴随实质纤维变性，这取决于缺血的严重程度。在第1天观察到中度和重度缺血诱导低氧诱导因子1仪和纤维生长因子2表达的提高，中度和重度缺血还可导致脂肪组织中的退行性改变（如细胞凋亡、坏死、巨噬细胞侵噬）。死亡细胞包括脂肪细胞，血管内皮细胞，血源性细胞，但不包括脂肪源性干／祖细胞。退行性改变后可观察到再生性改变，包括血管形成、脂肪形成和细胞的增殖（包括脂肪源性干／祖细胞、血管内皮细胞和血细胞）。笔者还发现，严重缺氧时在体外用实验方法分化的脂肪细胞发生凋亡和／或坏死，而脂肪源性干／祖细胞仍然存活。结论脂肪组织严重缺血／缺氧导致退行性改变，继而发生相应的组织重构。在缺血环境下，脂肪细胞很容易死亡，而脂肪源性干／祖细胞却非常活跃，并有助于组织修复。

关键词：干/祖细胞脂肪细胞局部缺血组织重构死亡血管内皮细胞退行性改变低氧诱导因子1

Education Programs for Invasive Procedures Involving Nurses: A Scoping Review

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Molecular Phylogeny and Evolution of Two Rhacophorus Species Endemic to Mainland Japan

Open Journal of Nursing（护理学期刊） 2024年第5期 200页

作者： Hiromi Shibuya Akiko Saito Masumi Mugiyama Noyuri Yamaji Chisato eto Satoshi Shibuya

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Asian Herpetological Research 2019年第2期10卷 86-104页

作者： Masafumi MATSUI Yasuhiro KAWAHARA Kanto NISHIKAWA Seiji IKEDA Koshiro eto Yusuke MIZUNO Graduate School of Human and Environmental Studies Kyoto UniversityYoshida Nihonmatsu-choSakyo-kuKyoto 606-8501Japan Hagi City Museum Horiuchi 355HagiYamaguchi 758-0057Japan Hiroshima University Museum Kagamiyama 1-1-1Higashi Hiroshima CityHiroshima 739-8524Japan Kitakyushu Museum of Natural History and Human History Higashida 2-4-1Yahatahigashi-kuKitakyushuFukuoka 805-0071Japan Takahari 4-1002 Meito-kuNagoya 465-0061Japan

We conducted molecular phylogenetic analyses of Japanese Rhacophorus species, especially of R. schlegelii and R. arboreus from the mainland, based on samples encompassing their known distribution ranges, and discussed about evolutionary history of Rhacophorus species within Japan. The common ancestor of Japanese Rhacophorus, except for R. owstoni from southern Ryukyus, was estimated to have diverged from a lineage occurring mainly in China about 7 MYBP. Both R. schlegelii and R. arboreus are genetically largely divergent between regions of eastern and western Japan, and this seems to have been promoted mainly by retreat to refugia. Retreats of the two species to different refugia sometimes in the past seem to have led restricted distribution of R. schlegelii in eastern and R. arboreus in western Japan, and brought their intraspecific variation patterns in morphology and breeding habit.

关键词： glacial period mitochondrial DNA differentiation refugia Rhacophorus arboreus Rhacophorus schlegelii

Phylogenetic placement and diet of homalopsid snake Miralia alternans (Ruess,1833)

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Zoological Research 2022年第5期43卷 734-737页

作者： Ibuki Fukuyama Tomonori Kodama Koshiro eto Masafumi Matsui Mohamad Yazid Hossman Kanto Nishikawa Graduate School of Human and Environmental Studies Kyoto UniversityYoshida-NihonmatsuSakyoKyoto 606-8501Japan Graduate School of Science Kyoto UniversityKitashirakawa-OiwakeSakyoKyoto 606-8502Japan Kitakyushu Museum of Natural History&Human History Higashida 2-4-1YahatahigashiKitakyushuFukuoka 805-0071Japan Research Developmentand Innovation DivisionForest Department SarawakKuchingSarawak 93250Malaysia Graduate School of Global Environmental Studies Kyoto UniversityYoshida-HonmachiSakyoKyoto 606-8501Japan

DEAR EDITOR,Miralia alternans(Ruess,1833)is distributed in Borneo,Sumatra,and Java in Southeast *** species is morphologically similar to Raclitia indica,a monotypic genus known from Peninsular ***,we collected a juvenile specimen of *** from Borneo,and report on its coloration in life and first prey item recovered from the *** also explored the phylogenetic position of the genus using molecular phylogenetic analysis of mitochondrial DNA(mtDNA)gene cytochrome b(cyt b)and nuclear gene prolactin receptor(PRLR),confirming *** as sister to ***,with the two genera exhibiting relatively high genetic divergence in cyt b(13.0%-13.1%).

关键词： EDIT fir Java

Lower Concentrations of Glucose or Insulin Decrease the Risk of Various Types of Cancer in the Long-Lived Ames Dwarf Mouse by Increasing the Expression of p27Kip1, a Cell-Cycle Repressor Protein

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American Journal of Molecular Biology 2020年第3期10卷 148-164页

作者： Isao eto Department of Nutrition Sciences (DNS) and Nutrition Obesity Research Center (NORC) University of Alabama at Birmingham Birmingham AL USA

Introduction. The molecular biological mechanism of the increased incidence of the various types of cancer in obesity or type 2 diabetes in rodents or humans has largely been resolved in recent years. By contrast, the molecular biological mechanism of the decreased, not increased, incidence of the various types of cancer in the homozygous long-lived Ames dwarf mice still remains unresolved. Objective. The first objective of the present study was to investigate whether the decrease in the incidence of cancer in the homozygous long-lived Ames dwarf mice is due to the increase, not decrease, in the expression of p27Kip1, a cell cycle repressor protein. The second objective was to investigate whether the decrease in the incidence of cancer in the homozygous long-lived Ames dwarf mice is due to the decrease, not increase, in the levels of glucose or insulin. Methods. To achieve these objectives, we first performed western immunoblot analysis of the hepatic expression of p27Kip1 protein. We then performed, using a human breast cancer cell line in vitro, the luciferase reporter plasmid assay to determine whether the translation initiation activity of the p27Kip1 mRNA is increased when the concentrations of either glucose or insulin are decreased. Results and Conclusion. The results of the first objective indicated that the hepatic expression of p27Kip1 protein was up-regulated in the homozygous long-lived Ames dwarf mice as expected. We also found that the lower concentrat

关键词： Cancer Glucose Insulin Caloric Restriction Long-Lived Ames Dwarf Mouse p27Kip1 Cell-Cycle Repressor Protein

Diverse Voices and Democratic Policymaking： What Causes Japan＇s Nuclear Phase-Out Plan to Fail？

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Journalism and Mass Communication 2016年第6期6卷 330-349页

作者： Mikiko eto Hosei University Tokyo Japan

The nuclear disaster at Fukushima has raised questions about the nature of democratic policymaking in Japan. Focusing on nuclear policymaking post-Fukushima, this paper considers ways in which the diversity of public opinion can be connected with legislative processes. The disaster forced the government to rethink existing nuclear policies. To infuse public voices into government policy, the Democratic Party of Japan （DPJ） government introduced a new method of public participation called ＂national debates＂, comprising a series of public hearings, mass public comments, and deliberative polling. The government also attached importance to anti-nuclear rallies and opinion polls conducted by the media. The national debates highlighted that the majority of Japanese people supported a nuclear-free society; as a result, the government drafted a new energy plan to phase out nuclear power by 2039. Owing to both domestic and international opposition, the plan failed to become law. Progress was also impeded by the public＇s contradictory demands. However, this paper posits that inefficient legislative deliberation was the main factor behind the policy failure. The Japanese case suggests that effective legislative deliberation is crucial to assessing and coordinating numerous divergent public voices.

关键词： public debates contradictory demands democracy nuclear policy Fukushima Japan