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A Cyborg Insect Reveals a Function of a Muscle in Free Flight

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Cyborg and Bionic Systems 2022年第1期2022卷 98-108页

作者： T.Thang Vo-Doan V.Than Dung hirotaka Sato Nanyang Technological University School of Mechanical and Aerospace EngineeringSingapore University of Freiburg Institute of Biology I Germany

While engineers put lots of effort,resources,and time in building insect scale micro aerial vehicles(MAVs)that fly like insects,insects themselves are the real masters of *** if we would use living insect as platform for MAV instead?Here,we reported a flight control via electrical stimulation of a flight musde of an insect-computer hybrid robot,which is the interface of a mountable wireless backpack controller and a living *** beetle uses indirect flight muscles to drive wing flapping and three major direct flight muscles(basalar,subalar,and third axilliary(3Ax)muscles)to control the kinematics of the wings for flight *** turning control was already achieved by stimulating basalar and 3Ax muscles,electrical stimu lation of subalar musdes resulted in braking and elevation control in *** also demonstrated around 20 degrees of contralateral yaw and roll by stimulating individual subalar *** both subalar muscles lead to an increase of 20 degrees in pitch and decelerate the flight by 1.5 m/s2 as well as an induce in elevation of 2m/s.

关键词： robot computer mount

Low haze transparent electrodes and highly conducting air dried films with ultra-long silver nanowires synthesized by one-step polyol method

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Nano Research 2014年第2期7卷 236-245页

作者： Teppei Araki Jinting Jiu Masaya Nogi hirotaka Koga Shijo Nagao Tohru Sugahara Katsuaki Suganuma Graduate School of Engineering Osaka University Ibaraki Osaka 567-0047 Japan Institute of Scientific and Industrial Research Osaka University Ibaraki Osaka 567-0047 Japan

Transparent electrodes made of silver nanowires （AgNWs） exhibit higher flexibility when compared to those made of tin doped indium oxide （ITO） and are expected to be applied in plastic electronics. However, these transparent electrodes composed of AgNWs show high haze because the wires cause strong light scattering in the visible range. Reduction of the wire diameter has been proposed as a way to weaken light scattering, although there have seldom been any studies focusing on the haze because of the difficulty involved in controlling the wire diameter. In this report, we show that the haze can be easily reduced by increasing the length of AgNWs with a large diameter. Ultra-long （u-long） AgNWs with lengths in the range of 20-100 μm and a maximum length of 230 μm have been successfully synthesized by adjusting the reaction temperature and the stirring speed of a one-step polyol process. Compared to typical AgNWs （with diameter and length of 70 nm and 10 μm, respectively） and ITO, a transparent electrode consisting of u-long AgNWs 91 nm in diameter demonstrated a low haze of 3.4%-1.6% and a low sheet resistance of 24-109 Ω/sq. at a transmittance of 94%-97%. Even when fabricated at room temperature without any post-treatment, the electrodes composed of u-long AgNWs achieved a sheet resistance of 19 Ω/sq, at a transmittance of 80%, which is six orders of magnitude lower than that of typical AgNWs.

关键词： ultra-long silver nanowires one-step synthesis transparent electrodes haze

Liquid biopsy of gastric cancer patients:Circulating tumor cells and cell-free nucleic acids

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World Journal of Gastroenterology 2014年第12期20卷 3265-3286页

作者： Masahiro Tsujiura Daisuke Ichikawa hirotaka Konishi Shuhei Komatsu Atsushi Shiozaki Eigo Otsuji Division of Digestive Surgery Department of SurgeryKyoto Prefectural University of Medicine

To improve the clinical outcomes of cancer patients, early detection and accurate monitoring of diseases are necessary. Numerous genetic and epigenetic alterations contribute to oncogenesis and cancer progression, and analyses of these changes have been increasingly utilized for diagnostic, prognostic and therapeutic purposes in malignant diseases including gastric cancer (GC). Surgical and/or biopsy specimens are generally used to understand the tumor-associated alterations; however, those approaches cannot always be performed because of their invasive characteristics and may fail to reflect current tumor dynamics and drug sensitivities, which may change during the therapeutic process. Therefore, the importance of developing a non-invasive biomarker with the ability to monitor real-time tumor dynamics should be emphasized. This concept, so called “liquid biopsy”, would provide an ideal therapeutic strategy for an individual cancer patient and would facilitate the development of “tailor-made” cancer management programs. In the blood of cancer patients, the presence and potent utilities of circulating tumor cells (CTCs) and cell-free nucleic acids (cfNAs) such as DNA, mRNA and microRNA have been recognized, and their clinical relevance is attracting considerable attention. In this review, we discuss recent developments in this research field as well as the relevance and future perspectives of CTCs and cfNAs in cancer patients, especially focusing on GC.

关键词： Gastric cancer Biomarker Liquid biopsy Circulating tumor cells Cell-free nucleic acids MicroRNA

Polyvinyl alcohol–potassium iodide gel probe to monitor the distribution of reactive species generation around atmospheric-pressure plasma jet

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Plasma Science and Technology 2023年第3期25卷 78-86页

作者： Trung Nguyen TRAN Min HU Tomohiro OGASAWARA Yuki IWATA Haruka SUZUKI Jin SAKAMOTO Masafumi AKIYOSHI hirotaka TOYODA Hiroto MATSUURA Department of Quantum and Radiation Engineering Osaka Prefecture University Department of Electronics Nagoya University Faculty of Chemistry Materials and BioengineeringKansai University Department of Quantum and Radiation Engineering Osaka Metropolitan University

This study proposes polyvinyl alcohol–potassium iodide(PVA–KI)as a novel gel chemical *** probe uses the reactions among PVA,KI,water,borax,and oxidative species to visualize the distribution of reactive *** method provides information regarding the distribution of reactive species by coloration on the gel *** effects of the surrounding gas phase on the distribution and diffusion of the reactive species are also investigated using the PVA–KI gel ***,the relationship between the irradiation distance and reactive species diffusion is determined on the surface of the PVA–KI probe with and without plastic *** the irradiation distance appropriately leads to an increase in the modified area as detected by the PVA–KI gel probe *** relative concentration distributions of the reactive species are also obtained from visualized color distributions measured using a ***,reactive species generation by long-scale line plasma is confirmed by the color reaction on the PVA–KI gel surface,with a greater area being covered by an atmospheric-pressure pulsed microwave line plasma source.

关键词： atmospheric-pressure plasma jet(APPJ) PVA–KI gel chemical probe argon plasma jets

Investigation on Effect of Composition on the Drying of Castables with Cement Binder

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China's Refractories 2024年第3期33卷 30-36页

作者： Bruno TOUZO hirotaka GOTO Uwe KLIPPEL Josh SAYRE Jeff BOGAN Calderys Paris 75016France Calderys Toyota 470-0308Japan Calderys Neuwied 56564Germany HWI West Mifflin 15122PAUSA

Monolithic refractory castables comprising a hydraulic bond are still used in a vast majority of cases because of their flexibility and robustness,despite many developments for chemical as well as non-cement castable *** drying can however be a challenge,in particular for deflocculated dense castables of the low cement castable *** publications have been released on this topic for the last ten years,but they often focused on the drying mechanisms or on the addition of drying *** paper presents some experimental results on the effect of the composition on the drying properties,especially on the effect of silicon carbide,used for its high thermal conductivity,and on the matrix *** also introduces two laboratory tests to study and iteratively improve the drying schedule of a given castable *** results show that the spalling resistance and the vapor pressure build-up are significantly influenced by the *** is also shown that the castable properties after drying can be altered if the heating rate is very high.

关键词： drying aggregate composition binding system heating rate

Reproducible reformation of a bilayer lipid membrane using microair bubbles

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Droplet 2023年第3期2卷 90-98页

作者： Izumi Hashimoto Toshihisa Osaki hirotaka Sugiura Hisatoshi Mimura Sho Takamori Norihisa Miki Shoji Takeuchi Artificial Cell Membrane Systems Group Kanagawa Institute of Industrial Science and TechnologyKanagawaJapan Department of Mechanical Engineering Keio UniversityKanagawaJapan Institute of Industrial Science The University of TokyoTokyoJapan Graduate School of Information Science and Technology The University of TokyoTokyoJapan

Planar bilayer lipid membranes(BLMs)are widely used as models for cell membranes in various applications,including drug discovery and ***,the nanometer-thick bilayer structure,assembled through hydrophobic interactions of amphiphilic lipid molecules,makes such BLM systems mechanically and electrically *** this study,we developed a device to reform BLMs using a microair *** device consists of a double well divided by a separator with a microaperture,where a BLM was formed by infusing a lipiddispersed solvent and an aqueous droplet into each well in *** the BLM ruptured,a microair bubble was injected from the bottom of the well to split the merged aqueous droplet at the microaperture,which resulted in the reformation of two lipid monolayers on the split *** bringing the two droplets into contact,a new BLM was *** angled step design was introduced in the BLM device to guide the bubble and ensure the splitting of the merged *** also elucidated the optimal bubble inflow rate for the reproducible BLM *** a 4-channel parallel device,we demonstrated the individual and repeatable reformation of *** approach will aid the development of automated and arrayed BLM systems.

关键词： bubble bringing unstable

Resilient conductive membrane synthesized by in-situ polymerisation for wearable non-invasive electronics on moving appendages of cyborg insect

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npj Flexible Electronics 2023年第1期7卷 109-118页

作者： Qifeng Lin Rui Li Feilong Zhang Kazuki Kai Zong Chen Ong Xiaodong Chen hirotaka Sato School of Mechanical and Aerospace Engineering Nanyang Technological University50 Nanyang AvenueSingapore 639798Singapore Innovative Center for Flexible Devices(iFLEX) Max Planck-NTU Joint Lab for Artificial SensesSchool of Materials Science and EngineeringNanyang Technological University50 Nanyang AvenueSingapore 639798Singapore CAS Key Laboratory of Bio-inspired Materials and Interfacial Science CAS Center for Excellence in NanoscienceTechnical Institute of Physics and ChemistryChinese Academy of SciencesBeijingP.R.China

By leveraging their high mobility and small size,insects have been combined with microcontrollers to build up cyborg insects for various practical ***,all current cyborg insects rely on implanted electrodes to control their movement,which causes irreversible damage to their organs and ***,we develop a non-invasive method for cyborg insects to address above issues,using a conformal electrode with an in-situ polymerized ion-conducting layer and an electron-conducting *** neural and locomotion responses to the electrical inductions verify the efficient communication between insects and controllers by the non-invasive *** precise“S”line following of the cyborg insect further demonstrates its potential in practical *** conformal non-invasive electrodes keep the intactness of the insects while controlling their *** the antennae,important olfactory organs of insects preserved,the cyborg insect,in the future,may be endowed with abilities to detect the surrounding environment.

关键词： conducting conformal organs

Serial changes in expression of functionally clustered genes in progression of liver fibrosis in hepatitis C patients

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World Journal of Gastroenterology 2008年第13期14卷 2010-2022页

作者： Yoshiyuki Takahara Mitsuo Takahashi Qing-Wei Zhang hirotaka Wagatsuma Maiko Mori Akihiro Tamori Susumu Shiomi Shuhei Nishiguchi Exploratory & Applied Pharmaceutical Research Department Pharmaceutical Company Ajinomoto Co. Inc. 1-1 Suzuki-cho Kawasaki-ku Kawasaki 210-8681 Japan Department of Nuclear Medicine Graduate School of Medicine Osaka City University Osaka Japan Division of Hepatobiliary and Pancreatic Diseases Department of Internal Medicine Hyogo College of Medicine Nishinomiya Japan

AIM: To investigate the relationship of changes in expression of marker genes in functional categories or molecular networks comprising one functional category or multiple categories in progression of hepatic fibrosis in hepatitis C (HCV) patients. METHODS: Marker genes were initially identified using DNA microarray data from a rat liver fibrosis model. The expression level of each fibrosis associated marker gene was analyzed using reverse transcription-polymerase chain reaction (RT-PCR) in clinical biopsy specimens from HCV-positive patients (n = 61). Analysis of changes in expression patterns and interactions of marker genes in functional categories was used to assess the biological mechanism of fibrosis. RESULTS: The profile data showed several biological changes associated with progression of hepatic fibrosis. Clustered genes in functional categories showed sequential changes in expression. Several sets of clustered genes, including those related to the extracellular matrix (ECM), inflammation, lipid metabolism, steroid metabolism, and some transcription factors important for hepatic biology showed expression changes in the immediate early phase (F1/F2) of fibrosis. Genes associated with aromatic amino acid (AA) metabolism, sulfur-containing AA metabolism and insulin/ Wnt signaling showed expression changes in the middle phase (F2/F3), and some genes related to glucose metabolism showed altered expression in the late phase of fibrosis (F3/F4). Therefore, molecular networks showing serial changes in gene expression are present in liver fibrosis progression in hepatitis C patients. CONCLUSION: Analysis of gene expression profiles from a perspective of functional categories or molecular networks provides an understanding of disease and suggests new diagnostic methods. Selected marker genes have potential utility for biological identification of advanced fibrosis.

关键词： Hepatitis C Liver fibrosis Marker gene Gene expression RT-PCR Molecular network Metabolism Transcription factor Diagnosis

Silver Nanowire Electrodes: Conductivity Improvement Without Post-treatment and Application in Capacitive Pressure Sensors

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Nano-Micro Letters 2015年第1期7卷 51-58页

作者： Jun Wang Jinting Jiu Teppei Araki Masaya Nogi Tohru Sugahara Shijo Nagao hirotaka Koga Peng He Katsuaki Suganuma State Key Laboratory of Advanced Welding and Joining Harbin Institute of Technology The Institute of Scientific and Industrial Research Osaka University

Transparent electrode based on silver nanowires(Ag NWs) emerges as an outstanding alternative of indium tin oxide film especially for flexible electronics. However, the conductivity of Ag NWs transparent electrode is still dramatically limited by the contact resistance between nanowires at high transmittance. Polyvinylpyrrolidone(PVP) layer adsorbed on the nanowire surface acts as an electrically insulating barrier at wire–wire junctions, and some devastating post-treatment methods are proposed to reduce or eliminate PVP layer, which usually limit the application of the substrates susceptible to heat or pressure and burden the fabrication with high-cost, time-consuming, or inefficient processes. In this work, a simple and rapid pre-treatment washing method was proposed to reduce the thickness of PVP layer from 13.19 to0.96 nm and improve the contact between wires. Ag NW electrodes with sheet resistances of 15.6 and 204 X sq-1have been achieved at transmittances of 90 and 97.5 %, respectively. This method avoided any post-treatments and popularized the application of high-performance Ag NW transparent electrode on more substrates. The improved Ag NWs were successfully employed in a capacitive pressure sensor with high transparency, sensitivity, and reproducibility.

关键词： Silver nanowire Pre-treatment Transparent electrode Pressure sensor