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Vascular endothelial growth factor and angiopoietins regulate sinusoidal regeneration and remodeling after partial hepatectomy in rats

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World Journal of Gastroenterology 2005年第46期11卷 7254-7260页

作者： Hiroaki Shimizu Noboru Mitsuhashi Masayuki Ohtsuka hiroshi ito Fumio Kimura Satoshi Ambiru Akira Togawa Hiroyuki Yoshidome Atsushi Kato Masaru Miyazaki Department of General Surgery Graduate School of Medicine Chiba University Chiba 260-0856 Japan

AIM： To study the regulatory mechanisms of sinusoida regeneration after partial hepatectomy. METHODS： We invesldgated the expression of angiopoietin （Ang）-1, Ang-2, Tie-2, and vascular endothelial growth factor （VEGF） in regenerating liver tissue by quantitative reverse-transcription polymerase chain reaction （RT- PCR） using a LightCycler （Roche Diagnostics） and also immunohistochemical staining after 70% hepatectomy in rats. In the next step, we isolated liver cells （hepatocytes, sinusoidal endothelial cell （SEC）, Kupffer cell, and hepatic stellate cells （HSC）） from regenerating liver tissue by in situ collagenase perfusion and counterflow elutriation, to determine potential cellular sources of these angiogenic factors after hepatectomy. Proliferation and apoptosis of SECs were also evaluated by proliferating cell nuclear antigen （PCNA） staining and the terminal deoxynucleotidyl transferase d-uridine triphosphate nick end labeling （TUNEL） assay, respectively. RESULTS： VEGF mRNA expression increased with a peak at 72 h after hepatectomy, decreasing thereafter. The expression of Ang-1 mRNA was present at detectable levels before hepatectomy and increased slowly with a peak at 96 h. Meanwhile, Ang-2 mRNA was hardly detected before hepatectomy, but was remarkably induced at 120 and 144 h. In isolated cells, VEGF mRNA expression was found mainly in the hepatocyte fraction. Meanwhile, mRNA for Ang-1 and Ang-2 was found in the SEC and HSC fractions, but was more prominent in the latter. The PCNA labeling index of SECs increased slowly, reaching a peak at 72 h, whereas apoptotic SECs were detected between 120 h and 144 h. CONCLUSION： Ang-Tie system, together with VEGF, plays a critical role in regulating balance between SEC proliferation and apoptosis during sinusoidal regeneration after hepatectomy. However, the VEGF system plays a more important role in the early phase of sinusoidal regeneration than angiopoietin/Tie system.

关键词： Vascular endothelial cell growth factor Angiopoietin Sinusoidal endothelial cell Hepatectomy Liver regeneration

Changes in growth factor and cytokine expression in biliary obstructed rat liver and their relationship with delayed liver regeneration after partial hepatectomy

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World Journal of Gastroenterology 2006年第13期12卷 2053-2059页

作者： Hironobu Makino Hiroaki Shimizu hiroshi ito Fumio Kimura Satoshi Ambiru Akira Togawa Masayuki Ohtsuka Hiroyuki Yoshidome Atsushi Kato Hideyuki Yoshitomi Shigeaki Sawada Masaru Miyazaki Department of General Surgery Graduate School of Medicine Chiba UniversityChibaJapan

AIM： To study the effects of obstructive jaundice on liver regeneration after partial hepatectomy. METHODS： Hepatocyte growth factor （HGF）, its receptor, c-Met, vascular endothelial growth factor （VEGF） and transforming growth factor-β1 （TGF-β1） mRNA expression in both liver tissue and isolated liver cells were investigated after biliary obstruction （BO） by quantitative reverse-transcription polymerase chain reaction （RT-PCR） using a LightCycler. Immunohistochemical staining for desmin and e-smooth muscle actin （α-SNA） was also studied. Regenerating liver weight and proliferating cell nuclear antigen （PCNA） labeling index, and growth factor expression were then evaluated after 70% hepatectomy with concomitant internal bUiary drainage in BO rats or sham-operated rats. RESULTS： Hepatic TGF-β1 mRNA levels increased significantly 14 days after BO, and further increased with duration of cholestasis. Meanwhile, HGF and VEGF tended to increase, but was not significant. In cell isolates, TGF-β1 mRNA was found mainly in the hepatic stellate cell （HSC） fraction. Immunohistochemical studies revealed an increased number of HSCs （desmin-positive cells） and activated HSCs （α-SMA-positive cells） in portal areas after BO. In a hepatectomy model, liver regeneration was delayed in BO rats, as compared to sham-operated rats. TGF-β1 mRNA was significantly up-regulated up to 48 h after hepatectomy, and the earlier HGF mRNA peak was lost in BO rats. CONCLUSION： BO induces HSCs proliferation and activation, leading to up-regulation of TGF-β1 mRNA and suppression of HGF mRNA in livers. These altered expression patterns may be strongly involved in delayed liver regeneration after hepatectomy with obstructive jaundice.

关键词： Biliary obstruction Liver regeneration Hepatocyte growth factor Transforming growth factor-β Hepatic stellate cells Hepatectomy

新城疫病毒某水禽分离株经鸡体传代后由非致病型转变为速发型的研究

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中国预防兽医学报 2003年第1期25卷 59-64页

作者：于圣青丁铲 NORIKO KISHIDA hiroshi ito hiroshi KIDA KOICHI OTSUKI YOSHIHIRO KAWAOKA TOSHIHIRO ito DepatmentofVeterinaryPublicHealth 中国农业科学院家禽科学研究所江苏扬州225003 LaboratoryofMicrobiology DepartmentofDiseaseControlGraduateSchoolofVeterinaryMedicineHokkaidoUniversity DepartmentofVeterinaryMicrobiologyFacultyofAgriculture DepartmentOfPathobiologicalSciences SchoolofVeterinaryMedicineUniversityofWisconsin-Madison

最近 ,一株无毒力的新城疫病毒 (NDV)在鸡体内繁殖时 ,变成了强毒株。但至今尚未能证实 ,经鸡体传代的野生水禽新城疫病毒是否也具有变成速发型毒株的能力。为了通过实验证明从水禽中分离的非致病型NDV可以转变为速发型病毒 ,我们通过... 详细信息

最近 ,一株无毒力的新城疫病毒 (NDV)在鸡体内繁殖时 ,变成了强毒株。但至今尚未能证实 ,经鸡体传代的野生水禽新城疫病毒是否也具有变成速发型毒株的能力。为了通过实验证明从水禽中分离的非致病型NDV可以转变为速发型病毒 ,我们通过在鸡体内传播鹅源性无毒力株 ,经气囊接种连续传代 9次 ,随后再在鸡脑内传代 5次。结果显示 ,该病毒的毒力变得很强 ,致死率可达 1 0 0 %。通过致病性试验证实 ,其具有典型的速发型病毒特征 ;融合蛋白裂解位点的序列分析表明 ,原始的分离株含有无毒力型毒株共有的裂解序列 :E_R_Q_E_R/L,而通过鸡体反复传代后 ,该序列变为致病性毒株共有的序列 :K_R_Q_K_R/F。这些结果表明 ,野生水禽中自然存在的无毒力毒株 ,具有无毒力株相应的序列 ,但当其在鸡群中传播时 ,则具有变成高致病性病毒的能力。同时研究表明 ,鸡体提供了该病毒从非致病型向致病型转变的选择机制。

关键词：水禽分离株鸡体传代新城疫病毒速发型非致病型致病性选择机制 F蛋白

The Efficacy and Safety of Continuous Popliteal Sciatic Nerve Block for the Relief of Pain Associated with Critical Limb Ischemia: A Retrospective Study

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Open Journal of Anesthesiology 2013年第10期3卷 433-437页

作者： Atsushi Hashimoto hiroshi ito Yuko Sato Yoshihiro Fujiwara Department of Anesthesiology Aichi Medical University Nagakute Japan

Background: Patients with critical limb ischemia (CLI) often suffer from severe pain. A continuous peripheral nerve block has been shown to provide effective analgesia for patients having lower limb surgery. We have been administering continuous sciatic nerve block (CSNB) for patients with CLI whose pain could not be relieved by other analgesic tools. The aim of this retrospective study was to investigate the efficacy and safety of CSNB for patients with CLI. Method: We retrospectively investigated 99 patients who received CSNB for the relief of severe pain in the lower limb associated with CLI. Patient demographics, neurological history, complications, and subjective evaluation of the effectiveness of CSNB were investigated from their clinical records. The distal tips of 108 catheters were cultured. Result: One hundred and seventy-two catheters were placed in 99 patients. More than 90% of the patients enjoyed considerable relief of severe pain. The analgesic effect of CSNB was greater in patients with older age and hemodialysis. Thirty-one catheters had positive bacterial colonization. However, no severe infectious complication was found. There was no relationship between the co-existence of diabetes and positive bacterial colonization. We encountered a patient with ASO and diabetes who suffered from persistent motor weakness and hypesthesia even after 3 months of CSNB placement. Conclusions: CSNB provided good pain control for patients with severe pain caused by CLI. Although catheters were frequently found to be colonized, infection at the catheter site was self-limiting even in patients with diabetes.

关键词： Critical Limb Ischemia Continuous Sciatic Nerve Block Pain Control

Rotator Cuff Reconstruction and Humeral Head Replacement Using Smaller Humeral Prostheses in Cuff Tear Arthropathy Patients under 70 Years of Age

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Open Journal of Orthopedics 2014年第10期4卷 263-272页

作者： Naoki Miyoshi Naoki Suenaga Naomi Oizumi Noboru Taniguchi hiroshi ito Department of Orthopaedic Surgery Asahikawa Medical University Asahikawa Japan The Upper Extremity Center of Joint Replacement and Endoscopic Surgery Hokushin Hospital Sapporo Japan Department of Orthopaedic Surgery Miyazaki University Miyazaki Japan

Background: Most reverse shoulder arthroplasty (RSA) studies have shown good improvement in arm elevation without improvements in external rotation (ER). In addition, high rates of complications after long-term RSA have been reported, suggesting that RSA should be limited to elderly patients, especially those who are older than 70 years old. Since 2001, we have developed a strategy of rotator cuff reconstruction with muscle transfer and humeral head replacement (HHR), using smaller humeral prostheses, in cuff tear arthropathy patients. The aim of the present study was to investigate the clinical outcome of our strategy in patients under 70 years of age who had irreparable rotator cuff tears and osteoarthritis. Materials and Methods: A total of 25 shoulders of 25 patients under 70 years of age (males, 15;females, 10) with irreparable cuff tears were treated with HHR and cuff reconstruction. The average age at the time of surgery was 64.3 years (range, 55 - 69) and the average follow-up period was 38.7 months (range, 24 - 72). The cuff defect was repaired using a partial subscapularis transfer in 14 shoulders;nine shoulders required a latissimus dorsi transfer, one required a pectoralis major transfer, and one required both a latissimus dorsi and a pectoralis major transfer. Clinical outcomes were assessed with the range of motion (ROM), UCLA score, Japanese Orthopaedic Association (JOA) score and postsurgical complications. Results: Shoulder pain was diminished in all patients after surgery. The preoperative UCLA and JOA scores were 13.1 and 47.0 respectively, improving to 28.6 and 81.5 respectively after surgery. Active forward flexion has improved from an average of 89.0° to 138.8°, and the ER improved from an average of 16.2° to 33.2°. No complications occurred after surgery. Conclusion: Anatomical reconstruction using smaller head humeral prostheses yielded favorable results and less complication, compared with RSA. Considering another advantage of ability to ret

关键词： Irreparable Rotator Cuff Tear Cuff Reconstruction Cuff Tear Arthropathy Humeral Head Replacement Using a Small Head

Fiber Structure Development in Bicomponent Fibers of Recycled PET Modified with a Chain Extender

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Fiber Structure Development in Bicomponent Fibers of Recycle...

Contrast uptake in primary hepatic angiosarcoma on gadolinium-ethoxybenzyl-diethylenetriamine pentaacetic acid-enhanced magnetic resonance imaging in the hepatobiliary phase

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作者： Kiyoshi Yamamoto Masahiro Katagiri hiroshi ito Takeshi Kikutani Department of Organic and Polymeric Materials Tokyo Institute of Technology Todyo Metropolitan Industrial Technology Research Institute Todyo Metropolitan Industrial Technology Research Institute Department of Organic and Polymeric Materials Tokyo Institute of Technology Department of Organic and Polymeric Materials Tokyo Institute of Technology

＜正＞1 Introduction Poly（ethylene terephthalate）（PET） flakes recycled from post consumer bottles are usually palletized using a twin-screw extruder prior to the further melt processing such as melt spinning. For the promotion of recycling, production of highly value-added products from the recycled polymers is inevitable. In this research, highly-crimped bicomponent PET fibers were prepared using the recycled PET modified with a chain extender.

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World Journal of Hepatology 2018年第1期10卷 166-171页

作者： Manabu Hayashi Satoshi Kawana Hirofumi Sekino Kazumichi Abe Naoki Matsuoka Masahito Kashiwagi Ken Okai Yukiko Kanno Atsushi Takahashi hiroshi ito Yuko Hashimoto Hiromasa Ohira Department of Gastroenterology Fukushima Medical University School of Medicine Department of Diagnostic Pathology Fukushima Medical University School of Medicine Department of Radiology Fukushima Medical University School of Medicine

Primary hepatic angiosarcoma is the most common malignant mesenchymal tumor of the liver. It has a poor prognosis and various appearances on magnetic resonance(MR) images. We report a case of hepatic angiosarcoma with a characteristic appearance on gadolinium-ethoxybenzyl-diethylenetriamine pentaacetic acid(Gd-EOB-DTPA)-enhanced MR imaging in the hepatobiliary phase. A 72-year-old man was admitted with a complaint of abdominal pain. Gd-EOBDTPA-enhanced MR imaging revealed a liver tumor that showed slight hyperintensity in the hepatobiliary phase. These findings suggested Gd-EOB-DTPA uptake in the tumor. An autopsy revealed the solid proliferation and sinusoidal spreading of hepatic angiosarcoma cells. Immunohistochemistry indicated that the tumor was negative for OATP1B3. Gd-EOB-DTPA uptake in the liver tumor in the hepatobiliary phase suggested sinusoidal tumor invasion with residual normal hepatocytes.

关键词： Hepatic angiosarcoma Gadoliniumethoxybenzyl-diethylenetriamine pentaacetic acid Cirrhosis Hepatocellular carcinoma

Visual Perception of Incomplete Human-Like Body Postures: A Near-Infrared Spectroscopy Study

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Journal of Behavioral and Brain Science 2014年第12期4卷 544-558页

作者： hiroshi ito Hirofumi Saito Misato Oi Tomoko Shiraishi Shuang Meng Victor Alberto Palacios Department of Psychology Faculty of Letters Aichi University Toyohashi Japan Department of Cognitive Informatics Graduate School of Information Science Nagoya University Nagoya Japan Department of Nursing College of Life and Health Sciences Chubu University Kasugai Japan

Previous studies have reported that the mirror neuron system plays a crucial role in social cognition. We examined whether the higher-order cognitive functions are involved in the activations in the mirror neuron area when we perceive simplified pseudo-postures. We measured 14 participants’ brain activation during the posture-recognition task using near-infrared spectroscopy. The participants’ task was to observe five sequentially presented target pseudo-postures and judge whether a test pseudo-posture was identical to one of the preceding five target pseudo-postures. The results in the majority of participants (n = 10/14) revealed that the activity in the inferior frontal mirror neuron area is modulated by perception of human-likeness, but not in the remaining four participants (n = 4/14). These results suggest that the degree of the activation of higher-order cognitive functions, which may be engaged in the inhibitory and/or facilitative processing of human body or bodily movement, leads to the distinctive activities in the inferior frontal mirror neuron area.

关键词： Near-Infrared Spectroscopy (NIRS) Mirror Neuron System Object Perception Human Body

Therapeutic Application of High-Frequency rTMS Combined with Intensive Occupational Therapy for Pediatric Stroke Patients with Upper Limb Hemiparesis: A Case Series Study

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Journal of Behavioral and Brain Science 2013年第2期3卷 188-193页

作者： Masachika Niimi Wataru Kakuda Toru Takekawa Ryo Momosaki Takatoshi Hara hiroshi ito Yumi Kameda Masahiro Abo Department of Rehabilitation Medicine The Jikei University School of Medicine Tokyo Japan

Low-frequency repetitive transcranial magnetic stimulation (LF-rTMS) applied to the non-lesional hemisphere is reported to significantly improve motor function of the affected upper limb in adult stroke patients with hemiparesis. For pediatric stroke patients, the beneficial effects of LF-rTMS have been already confirmed in a randomized controlled study. However, there is no report of therapeutic application of high-frequency rTMS (HF-rTMS) in this patient population. In this case series study, we introduced HF-rTMS combined with intensive occupational therapy (OT) in two pediatric hemiparetic patients. We studied two children (8- and 9-year-old boys, both right-handed) with post-stroke upper limb hemiparesis (chronic phase). Both patients underwent 22 treatment sessions of HF-rTMS/OT during 15-day hospitalization. The HF-rTMS was applied over the lesional motor cortex at a frequency of 10Hz for 15 minutes in each session. One session of intensive OT consisted of 30-min one-to-one training and 30-min self-exercise. Motor function of the affected upper limb was serially evaluated with Fugl-Meyer Assessment (FMA), Wolf Motor Function Test (WMFT), Simple Test for Evaluating hand Function (STEF), and Ten-second Test. Neither of the patients showed any adverse effects. Both patients showed improvement of motor function in the affected upper limb and were able to use the affected upper limb in some activities of daily living. In the two post-stroke pediatric patients, HF-rTMS/OT was safe and improved upper limb muscle function. Confirmation of these effects in a larger population is needed.

关键词： Pediatric Stroke Repetitive Transcranial Magnetic Stimulation Occupational Therapy Upper Limb Hemiparesis Motor Recovery