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Efficient Production of L-Theanine Using Immobilized Recombinant <i>Escherichia coli</i>Cells Expressing a Modified γ-Glutamyltranspeptidase Gene from <i>Pseudomonas nitroreducens</i>

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Advances in Biological Chemistry 2020年第6期10卷 157-171页

作者： Putthapong Phumsombat Chiharu Sano hiroki Ikezoe Junji hayashi Takafumi Itoh Takao Hibi Mamoru Wakayama Department of Biotechnology College of Life Sciences Ritsumeikan University Shiga Japan Graduate School of Technology Industrial and Social Sciences Tokushima University Tokushima Japan Department of Bioscience and Biotechnology Fukui Prefectural University Fukui Japan

L-Theanine (γ-glutamylethylamide) is a naturally occurring amino acid derivative known to have several beneficial physiological effects as a diet supplement, and to give an umami taste when used as a food additive. The compound is industrially produced by γ-glutamyltranspeptidase from Pseudomonas nitroreducens (PnGGT). Using recombinant PnGGT, we have shown previously that Trp385, Phe417, and Trp525 are key amino acid residues for recognition of acceptor substrates at the PnGGT active site. Here, we demonstrate that a recombinant W525D mutant of PnGGT produces L-theanine from ethylamine and L-glutamine more efficiently than wild-type PnGGT, attributable to an increased ratio of transfer activity to hydrolysis activity. An efficient production of L-theanine was achieved by immobilizing Escherichia coli cells expressing the W525D PnGGT mutant (E. coli-W525D) using 2% alginate as the supporting material. The highest L-theanine production using immobilized E. coli-W525D, representing a conversion rate of 90%, was achieved in optimal reaction conditions of pH 10, 40°C, and a substrate molar ratio of L-glutamine to ethylamine of 1:10. The immobilized E. co

关键词： L-Theanine γ-Glutamyltranspeptidase Pseudomonas nitroreducens Escherichia coli Immobilization

Inhibitory effects of carbon dioxide insufflation on pneumoperitoneum and bowel distension after percutaneous endoscopic gastrostomy

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World Journal of Gastroenterology 2012年第27期18卷 3565-3570页

作者： Shinji Nishiwaki Hiroshi Araki Motoshi hayashi Jun Takada Masahide Iwashita Atsushi Tagami Hiroo Hatakeyama Takao hayashi Teruo Maeda Koshiro Saito Department of Internal Medicine Nishimino Kosei HospitalGifu 503-1394Japan Department of Gastroenterology Graduate School of MedicineGifu UniversityGifu 501-1194Japan

AIM:To evaluate the inhibitory effects of carbon dioxide (CO2) insufflation on pneumoperitoneum and bowel distension after percutaneous endoscopic gastrostomy (PEG).METHODS:A total of 73 consecutive patients who were undergoing PEG were enrolled in our *** eliminating 13 patients who fitted our exclusion criteria,60 patients were randomly assigned to either CO2 (30 patients) or air insufflation (30 patients) *** was performed by pull-through technique after threepoint fixation of the gastric wall to the abdominal wall using a gastropexy *** blood gas analysis was performed immediately before and after the *** X-ray was performed at 10 min and at 24 h after PEG to assess the extent of bowel *** computed tomography was performed at 24 h after the procedure to detect the presence of *** outcomes of PEG for 7 d postprocedure were also ***:Among 30 patients each for the air and the CO2 groups,PEG could not be conducted in 2 patients of the CO2 group,thus they were *** of the remaining 58 patients showed that the patients' backgrounds were not significantly different between the two *** elevation values of arterial partial pressure of CO2 in the air group and the CO2 group were 2.67 mmHg and 3.32 mmHg,respectively (P = 0.408).The evaluation of bowel distension on abdominal X ray revealed a significant decrease of small bowel distension in the CO2 group compared to the air group (P < 0.001) at 10 min and 24 h after PEG,whereas there was no significant difference in large bowel distension between the two *** was observed only in the air group but not in the CO2 group (P = 0.003).There were no obvious differences in the laboratory data and clinical outcomes after PEG between the two ***:There was no adverse event associated with CO2 ***2 insufflation is considered to be safer and more comfortable for PEG patients bec

关键词： Percutaneous endoscopic gastrostomy Car-bon dioxide insufflation Pneumoperitoneum Abdomi-nal distension Randomized control study

Basic principles of fracture management in small animals

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畜牧与兽医 2012年第S2期44卷 14-17页

作者： Kei hayashi Orthopedic Surgery University of California

1 General Strategy for Fracture Treatment The goal of this lecture is to help you develop a rational approach to choosing a repair method for the fractures you *** the proper choice is vital to your success of fracture *** goal of fracture stabilization is to achieve complete bone healing while maintaining optimum function and achieving this as quickly as *** achieve this goal,there is a helpful guideline using a step-wise approach:*** patient:Look at the whole patient and assess signalment,history,and problem list to determine the bio-

关键词： lecture stabilization helpful vital repair Fracture maintaining quickly healing rational

Numerical and Experimental Investigation on Heat PropagationThrough Composite Sinter Bed With Non-Uniform Voidage:Part Ⅱ Prediction of Process Efficiency

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Journal of Iron and Steel Research International 2010年第10期17卷 1-6页

作者： Sergey V Komarov Hiroyuki Shibata Naohito hayashi Eiki Kasai Nippon Light Metal Company Tohoku University

The results of numerical simulation of the mosaic embedding iron ore sintering （MEBIOS） process according to the model presented in the first part of the study are introduced. The main objective of the second part is the elucidation of key factors influencing the process efficiency, particularly the sintering completeness, velocity of the heat wave propagation and maximum temperature achievable in the pellet during the wave propagation. Numerical results reveal that the mass fraction of coal, diameter of coal particles and air inlet velocity are of prime importance in determining efficiency of the MEBIOS sintering process.

关键词： sintering MEBIOS process heat propagation mechanism numerical simulation process efficiency

Effect of calcium silicate on nutrient use of lowland rice and greenhouse gas emission from a paddy soil under alternating wetting and drying

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Pedosphere 2020年第4期30卷 535-543页

作者： Hyun-Hwoi KU Keiichi hayashi Ruth AGBISIT Gina VILLEGAS-PANGGA Crop and Environmental Sciences Division International Rice Research InstituteLos BanosLaguna 4031(Philippines) Fanning Systems and Soil Resources Institute Agricultural Systems ClusterCollege of AgricultureUniversity of the Philippines at Los BanosLaguna 4031(Philippines) Crop Livestock and Environment DivisionJapan International Research Center for Agricultural Sciences1-1 OhwashiTsukubalbaraki 305-0851(Japan)

In intensively irrigated rice cultivation,plant-available silicon(Si)is a crucial nutrient for improving rice *** a source of Si,calcium silicate(CaSiO3)was amended to evaluate the effect of silicate fertilizer on rice production,nitrogen(N)use efficiency,and greenhouse gas(GHG)emission under alternating wetting and drying in a pot experiment using a tropical soil from a paddy field of the International Rice Research Institute(IRRI)in the *** levels of CaSiO3 amendment,0,112.7,224.5,and 445.8 kg ha^-1,with the recommended N rate were *** results showed that although CaSiO3amendment of 112.7 kg ha^-1resulted in higher rice straw,improved N use efficiency,and reduced N2O emission,there was no difference in grain yield among the four levels of CaSiO3 amendment owing to relatively lower harvest ***,CaSiO3 amendment showed a reverse trend between CH4 and N2O emissions as it reduced N2O emission while led to significantly increased CH4 emission and global warming ***,CaSiO3 amendment was a possible alternative to improve N use efficiency and increase rice straw biomass,but it needs to be reviewed in line with grain yield production and GHG *** is also imperative to test an optimal method of silicate fertilizer amendment in future research in order to compromise a negative impact in tropical soils.

关键词： CH4 emission N use efficiency N2O emission plant-available Si rice straw biomass silicate fertilizer tropical soil

Stable Vertical Ladder Climbing with Rung Recognition for a Four-limbed Robot

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Journal of Bionic Engineering 2021年第4期18卷 786-798页

作者： Xiao Sun Kenji Hashimoto Shota hayashi Masahiro Okawara Takashi Mastuzawa Atsuo Takanishi Department of Mechatronics University of YamanashiYamanashiKofu400-8511Japan Department of Mechanical Engineering Informatics Meiji UniversityTokyo214-8571Japan Faculty of Science and Engineering Waseda UniversityTokyo162-8480Japan

This paper proposes a system for stable ladder climbing of the human-sized four-limbed robot“WAREC-1”,including the following 3 components:(a)Whole-body motion planning;(b)Rung recognition system and(c)Reaction force *** 3 components guarantee appropriate ladder climbing motion,successful rung grub and proper reaction force distribution at contact points throughout the climbing motion,*** this system,(1)Stable ladder climbing in 2-point contact gait by a human-sized robot and(2)Successful and stable climbing of an irregular ladder(with a higher or inclined rung)in both 3-point and 2-point contact gait with the capability of recognizing the target rung and the corresponding motion planning are realized,which have rarely been realized by former ***,experiment results and data of the robot ladder climbing are also presented to evaluate the proposed system.

关键词： legged robot ladder climbing stability proximity sensor feedback force control

Catalytic Asymmetric Carbon-Carbon Forming Reactions Catalyzed Chiral Schiff Base-Metal Complexes

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复旦学报（自然科学版） 2007年第5期46卷 573-574页

作者： Takanori Tanaka Masahiko hayashi Department of Chemistry Graduate School of ScienceKobe University

1 Results In 1991, we disclosed the novel asymmetric catalysts prepared from chiral Schiff base and titanium alkoxide in the reaction of asymmetric silylcyanation of aldehydes (eq.1)[1]. Since our first report, chiral Schiff base-metal complex was proven to be efficient in a variety of asymmetric reactions. We reported the first example of enantioselective addition of diketene to aldehydes promoted by chiral Schiff base-titanium alkoxide complexes (eq.2)[2]. The products of this reaction have been cove...

关键词： asymmetric carbon-carbon forming reaction Schiff base dialkylzinc enantioselective reaction

Transition of Groundwater and Subsurface Thermal Environment in the Tokyo Metropolitan Area,Japan

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地学前缘 2009年第S1期16卷 15-16页

作者： Akinobu Miyakoshi Takeshi hayashi Institute for Geo-Resources and Environment National Institute of Advanced Industrial Science and Technology Faculty of Education and Human Studies Akita University

Information on three-dimensional subsurface temperature distribution and its change were examined by measuring of temperature-depth profiles at observation wells in 2001-2002 and 2005-2006,to evaluate the subsurface t... 详细信息

关键词： groundwater flow subsurface temperature urban subsurface environment urbanization Tokyo

Statin as a therapeutic agent in gastroenterological cancer

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World Journal of Gastrointestinal Oncology 2022年第1期14卷 110-123页

作者： Norio Uemura Hiromitsu hayashi Hideo Baba Department of Gastroenterological Surgery Graduate School of Medical SciencesKumamoto UniversityKumamoto 860-8556Japan

Statins inhibit 3-hydroxy-3-methylglutaryl-CoA reductase,the rate-limiting enzyme of the mevalonate pathway,and are widely used as an effective and safe approach handle *** mevalonate pathway is a vital metabolic pathway that uses acetyl-CoA to generate isoprenoids and sterols that are crucial to tumor growth and *** studies have indicated that statins improve patient prognosis in various *** research on the mechanisms underlying the antitumor effects of statins is *** development of new anti-cancer drugs is progressing,but increasing medical costs from drug development have become a major *** available,inexpensive and well-tolerated drugs like statins have not yet been successfully repurposed for cancer *** the cancer patients that may benefit from statins is key to improved patient *** review summarizes recent advances in statin research in cancer and suggests important considerations for the clinical use of statins to improve outcomes for cancer patients.

关键词： Statin HMG CoA reductase inhibitor Mevalonate pathway Cancer