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Establishment of mouse intestinal myofibroblast cell lines

World Journal of Gastroenterology 2013年第17期19卷 2629-2637页

作者： hideyoshi kawasaki Takashi Ohama Masatoshi Hori Koichi Sato Laboratory of Veterinary Pharmacology Joint Faculty of Veterinary Medicine Yamaguchi University Department of Veterinary Pharmacology Graduate School of Agriculture and Life Sciences the University of Tokyo

AIM:To establish novel intestinal myofibroblast (IMF) cell lines from mouse colonic mucosa and investigate their biological characters. METHODS:Primary IMFs were isolated from mucosal tissues of mouse colon that was denuded of epithelial cells and smooth muscle layer. For immortalization, primary IMFs were transfected with simian virus 40 large T antigen (designated as LmcMF). We also isolated some primary IMFs that spontaneously became immortalized without transfection (designated as SmcMF). To check immortality and normality of these cells, we examined their proliferative ability and contact inhibition. Moreover, the expression levels of proteins characterizing IMFs [including α-smooth muscle actin (α-SMA), vimentin, desmin, and type Ⅰ collagen] and proteins associated with the immune response [such as toll-like receptor 4 (TLR-4), CD14, MD2, IκBα, and p-p38] were determined by Western blotting. The localization of several myofibroblast protein markers was also detected by immunofluorescence staining. RESULTS:The cell growth assay results show that both LmcMF and SmcMF cells proliferated logarithmically at least up to passage 20. In addition, the contact inhibition assays show that LmcMF and SmcMF stopped growing after the cells reached confluence. These data suggest that these 2 types of cells were immortalized without losing contact inhibition of growth. Moreover, both LmcMF and SmcMF, like primary IMFs, showed spindle-shaped appearance. The expression levels of key myofibroblast protein markers, including α-SMA, vimentin, and desmin, were also examined by the Western blotting and immunofluorescence analyses. Our results show that these cells were positive for α-SMA and vimentin, but not desmin, as well as that both LmcMF and SmcMF expressed type Ⅰ collagen at a lower level than primary IMFs. Finally, we investigated the expression level of lipopolysaccharide (LPS) receptor-related proteins, as well as the response of the cells to LPS treatment. We found that th

关键词： Cell line Colon Lipopolysaccharide Mouse Myofibroblast

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Enzyme-linked immunosorbent assay of 3 Screen Islet Cell Autoantibody in patients with autoimmune thyroid disease

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World Journal of Diabetes 2024年第5期15卷 935-944页

作者： Eiji kawasaki Hidekazu Tamai Takahiro Fukuyama Yoko Sagara Ryutaro Hidaka Aira Uchida Masayuki Tojikubo Narihito Tatsumoto Yuko Akehi Yuji Hiromatsu The Diabetes Thyroid and Endocrine CenterShin-Koga HospitalKurume 830-8577Japan

BACKGROUND In recent years,the emergence of multiplex technology that can simultaneously measure multiple anti-islet autoantibodies has become particularly valuable for the staging and early diagnosis of immune-mediated type 1 diabetes(T1D).While it has been established that 20%-30%of T1D patients suffer from autoimmune thyroid disease(AITD),there is limited available data regarding the presence of anti-islet autoantibodies in AITD *** commercially available anti-islet autoantibodies,glutamic acid decarboxylase 65 autoantibodies(GADAs)are often the first marker measured in general clinical *** To investigate the frequency of anti-islet autoantibodies in AITD *** Our study involved four hundred ninety-five AITD patients,categorized into three distinct groups:AITD with T1D(n=18),AITD with phenotypic type 2 diabetes(T2D)(n=81),and AITD without diabetes(n=396),and the enzyme-linked immunosorbent assay(ELISA)was employed to determine the frequencies of 3 Screen Islet Cell Autoantibody(3 Screen ICA),GADA,insulinoma-associated antigen-2 autoantibodies(IA-2As),and zinc transporter 8 autoantibodies(ZnT8As)within these *** The frequency of 3 Screen ICA in AITD patients with T1D,T2D,and those without diabetes were 88.9%,6.2%,and 5.1%,respectively,with no significant difference seen between the latter two ***,the frequency of 3 Screen ICA was 11.1%higher in AITD patients with T1D,1.3%higher in AITD patients with T2D,and 1.1%higher in AITD patients without diabetes compared to GADA,***,12.5%,20.0%,and 20.0%of the 3 Screen ICA-positive patients were negative for ***,1.3%of the AITD patients who tested negative for 3 Screen ICA in both the AITD with T2D and non-diabetic AITD groups were found to be positive for individual *** the 3 Screen ICA-positive patients,there was a significantly higher proportion of individuals with multiple autoantibodies in AITD patients with T1D com

关键词： Anti-islet autoantibodies Autoimmune thyroid disease Real-world practice Retrospective study Type 1 diabetes

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Review Integration of smartphone technology and artificial intelligence for advanced ophthalmic care:A systematic review

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Advances in Ophthalmology Practice and Research 2024年第3期4卷 120-127页

作者： Kai Jin Yingyu Li Hongkang Wu Yih Chung Tham Victor Koh Yitian Zhao Ryo kawasaki Andrzej Grzybowski Juan Ye Eye Center The Second Affiliated Hospital of Zhejiang University School of MedicineZhejiang Provincial Key Laboratory of OphthalmologyZhejiang Provincial Clinical Research Center for Eye DiseasesZhejiang Provincial Engineering Institute on Eye DiseasesHangzhouChina Centre for Innovation and Precision Eye Health National University of SingaporeSingapore Department of Ophthalmology National University of SingaporeSingapore Singapore Eye Research Institute Singapore National Eye CentreSingapore Ophthalmology and Visual Science Academic Clinical Program Duke-NUS Medical SchoolSingapore Cixi Institute of Biomedical Engineering Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingboChina Ningbo Eye Hospital NingboChina Zhejiang International Scientific and Technological Cooperative Base of Biomedical Materials and Technology Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingboChina Zhejiang Engineering Research Center for Biomedical Materials Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingboChina Division of Public Health Department of Social MedicineGraduate School of MedicineOsaka UniversityOsakaJapan Artificial Intelligence Center for Medical Research and Application Osaka University HospitalOsakaJapan Institute for Research in Ophthalmology Foundation for Ophthalmology DevelopmentPoznanPoland A R T I C L

Background:The convergence of smartphone technology and artificial intelligence(AI)has revolutionized the landscape of ophthalmic care,offering unprecedented opportunities for diagnosis,monitoring,and management of ocular ***,there is a lack of systematic studies on discussing the integration of smart-phone and AI in this *** text:This review includes 52 studies,and explores the integration of smartphones and AI in ophthalmology,delineating its collective impact on screening methodologies,disease detection,telemedicine initiatives,and patient *** collective findings from the curated studies indicate promising performance of the smartphone-based AI screening for various ocular diseases which encompass major retinal diseases,glaucoma,cataract,visual impairment in children and ocular surface ***,the utilization of smartphone-based imaging modalities,coupled with AI algorithms,is able to provide timely,efficient and cost-effective screening for ocular *** modality can also facilitate patient self-monitoring,remote patient monitoring and enhancing accessibility to eye care services,particularly in underserved *** involving data pri-vacy,algorithm validation,regulatory frameworks and issues of trust are still need to be ***,evaluation on real-world implementation is imperative as well,and real-world prospective studies are currently ***:Smartphone ocular imaging merged with AI enables earlier,precise diagnoses,personalized treat-ments,and enhanced service accessibility in eye *** is crucial to navigate ethical and data security challenges while responsibly leveraging these innovations,promising a potential revolution in care access and global eye health equity.

关键词： Artificial intelligence Smartphone imaging Ophthalmology Telemedicine Ocular disease management

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多元文化共生时代的“简明日语”

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语言战略研究 2024年第4期9卷 8-9页

作者：河崎深雪日本《社会语言科学》杂志

“简明日语”(Yasashii Nihongo)是1995年阪神淡路大地震后,作为应紧急状态下向外国人传达信息的需要而诞生的。“简明日语”已有20多年的历史,在理论和实践上取得了丰硕的成果和多样化发展,主要表现在6个方面。一、应急领域应用。弘前... 详细信息

“简明日语”(Yasashii Nihongo)是1995年阪神淡路大地震后,作为应紧急状态下向外国人传达信息的需要而诞生的。“简明日语”已有20多年的历史,在理论和实践上取得了丰硕的成果和多样化发展,主要表现在6个方面。一、应急领域应用。弘前大学佐藤和之等学者1999年发表了《在发生灾害时为向外国人使用的日语文本方案:在广播、海报等上使用的简明日语表达方式》,接下来又发布了一系列研究成果,并研制了日语简化标准。东北大学伊藤彰则开发了日语简化支援系统“Yansisu”。

关键词：日语表达支援系统多样化发展传达信息紧急状态多元文化共生研究成果东北大学

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Non-invasive gene delivery across the blood-brain barrier:present and future perspectives

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Neural Regeneration Research 2022年第4期17卷 785-787页

作者： Seigo Kimura hideyoshi Harashima Laboratory for Molecular Design of Pharmaceutics Faculty of Pharmaceutical SciencesHokkaido UniversitySapporoJapan Laboratory of Innovative Nanomedicine Faculty of Pharmaceutical SciencesHokkaido UniversitySapporoJapan

The aging of society has arrived,and is accompanied by an increase in the absolute numbers of patients with neurological disorders,such as Alzheimer's and Parkinson's diseases(Feigin et al.,2020).Such diseases,particularly Alzheimer's disease and other forms of dementia,affect not only the patients themselves,but also the people around them,including family members and care *** a result,such neurological disorders are thought to carry a larger social burden compared to other *** most critical point in the current situation is that there is no effective treatment despite the fact that the number of patients increase with the aging of the population.

关键词： diseases Alzheimer carry

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日本法人处罚的现状与课题——组织模式并用说与企业系统过失责任的提案

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北京社会科学 2024年第2期 55-61页

作者：川崎友巳段宇衡(译) 同志社大学京都大学

日本对法人犯罪采“两罚制”,但该制度存在着处罚标准不明确、处罚根据不明晰及预期效果难以达成的问题,故应考虑代替方案。但作为传统立法论的企业组织体责任论与法人行为责任说均存在着缺陷。作为因应,应提倡组织模式并用说。该说在... 详细信息

日本对法人犯罪采“两罚制”,但该制度存在着处罚标准不明确、处罚根据不明晰及预期效果难以达成的问题,故应考虑代替方案。但作为传统立法论的企业组织体责任论与法人行为责任说均存在着缺陷。作为因应,应提倡组织模式并用说。该说在考虑法人作为“被认可为在法律上具有与自然人同样权限与能力的存在”及“组织体之性质”的基础上,承认个人模式与组织模式的各自意义并一并采用。一方面,该说基于对责任主义中主观责任之原则的尊重,将能与法人等同的自然人的意思与行为视为法人的意思与行为,并追究法人的故意、过失责任;另一方面,该说基于企业系统过失责任,赋予企业法人建设相应系统的法律义务。

关键词：法人处罚个人模式组织模式企业系统过失责任

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Functionally Graded Dual-nanoparticulate-reinforced Aluminium Matrix Bulk Materials Fabricated by Spark Plasma Sintering

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Journal of Materials Science & Technology 2014年第8期30卷 736-742页

作者： Hansang Kwon Marc Leparoux Akira kawasaki Department of Materials System Engineering Pukyong National University365 Sinsenro608-759 BusanKorea Empa Swiss Federal Laboratories for Materials Science and TechnologyAdvanced Materials ProcessingFeuerwerkerstrasse 39CH-3602 ThunSwitzerland Department of Materials Processing Engineering Graduate School of EngineeringTohoku University980-8579 SendaiJapan

Functionally graded（FG） carbon nanotubes（CNT） and nano-silicon carbide（nSiC） reinforced aluminium（Al）matrix composites have been successfully fabricated using high-energy ball milling followed by solid-state spark plasma sintering *** CNTs were well-dispersed in the Al particles using the nSiC as a solid mixing *** different types of multi-walled CNTs were used to add different amounts of CNTs in the same *** ball milled Al—CNT—nSiC and Al—CNT powder mixtures were fully densified and demonstrated good adhesion with no serious microcracks and pores within an FG multilayer *** layer contained different amounts of the CNTs,and the nSiC additions showed different microstructures and *** is possible to control the characteristics of the FG multilayer composite through the efficient design of an Al—CNT—nSiC gradient *** concept offers a feasible approach for fabricating the dualnanoparticulate-reinforced Al matrix nanocomposites and can be applied to other scenarios such as polymer and ceramic systems.

关键词： Carbon nanotubes(CNT) Silicon carbide High-energy ball milling Spark plasma sintering(SPS) Functionally graded materials(FGM)

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Lensless complex amplitude demodulation based on deep learning in holographic data storage

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Opto-Electronic Advances 2023年第3期6卷 42-56页

作者： Jianying Hao Xiao Lin Yongkun Lin Mingyong Chen Ruixian Chen Guohai Situ hideyoshi Horimai Xiaodi Tan College of Photonic and Electronic Engineering Key Laboratory of Opto-Electronic Science and for Medicine of Ministry of EducationFujian Provincial Key Laboratory of Photonics TechnologyFujian Provincial Engineering Technology Research Center of Photoelectric Sensing ApplicationFujian Normal UniversityFuzhou 350117China Shanghai Institute of Optics and Fine Mechanics Chinese Academy of SciencesShanghai 201800China HolyMine Corporation 2032-2-301 OokaNumazuShizuoka 410-0022Japan

To increase the storage capacity in holographic data storage(HDS),the information to be stored is encoded into a complex *** and accurate retrieval of amplitude and phase from the reconstructed beam is necessary during data readout in *** this study,we proposed a complex amplitude demodulation method based on deep learning from a single-shot diffraction intensity image and verified it by a non-interferometric lensless experiment demodulating four-level amplitude and four-level *** analyzing the correlation between the diffraction intensity features and the amplitude and phase encoding data pages,the inverse problem was decomposed into two backward operators denoted by two convolutional neural networks(CNNs)to demodulate amplitude and phase *** experimental system is simple,stable,and robust,and it only needs a single diffraction image to realize the direct demodulation of both amplitude and *** our investigation,this is the first time in HDS that multilevel complex amplitude demodulation is achieved experimentally from one diffraction intensity image without iterations.

关键词： holographic data storage complex amplitude demodulation deep learning computational imaging

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Endoscopic findings can predict the efficacy of leukocytapheresis for steroid-naive patients with moderately active ulcerative colitis

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World Journal of Gastroenterology 2008年第34期14卷 5316-5321页

作者： Yasushi Umehara Masatoshi Kudo Masanori kawasaki Division of Gastroenterology and Hepatology Department of Internal Medicine Kinki University School of Medicine

AIM:To investigate the therapeutic usefulness of leukocytapheresis (LCAP; Cellsoba) in steroid-naive patients with moderately active ulcerative colitis (UC). METHODS: Eighteen steroid-naive patients with moderately active UC received one LCAP session every week for fi ve consecutive weeks. RESULTS: The remission rate 8 weeks after the last LCAP session was 61.1% (11/18). All three patients with deep ulcers showed worsening after LCAP. For the remaining 15 patients, who had erosions or geographic ulcers, the average clinical activity index (CAI) score dropped significantly from 9.4 to 3.8 eight weeks after the last LCAP session (t = 4.89, P = 0.001). The average C-reactive protein (CRP) levels before and after LCAP were 1.2 mg/dL and 1.0 mg/dL, respectively. Of the patients with erosions, geographic ulcers, and deep ulcers, 100% (9/9), 33.3% (2/6), and 0% (0/3) were in remission 8 weeks after the last LCAP session, respectively (χ2 = 7.65, P < 0.005). Forty- eight weeks after the last LCAP session, the remission rates for patients with erosions and geographic ulcers were 44.4% (4/9) and 16.7% (1/6), respectively. Only one patient suffered a mild adverse event after LCAP (nausea). CONCLUSION: LCAP is a useful and safe therapyfor steroid-naive UC patients with moderate disease activity. Moreover, the effi cacy of the treatment can be predicted on the basis of endoscopic fi ndings.

关键词： Ulcerative colitis Steroid-naive Leukocytapheresis Efficacy Endoscopic findings

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Media Preference, Information Needs, and the Language Proficiency of Foreigners in Japan after the 2011 Great East Japan Earthquake

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International Journal of Disaster Risk Science 2018年第1期9卷 1-15页

作者： Akiyuki kawasaki Michael Henry Kimiro Meguro Department of Civil Engineering The University of Tokyo Division of Field Engineering for the Environment Faculty of Engineering Hokkaido University International Center for Urban Safety Engineering Institute of Industrial Science The University of Tokyo

After the 2011 Great East Japan Earthquake, the Japanese government identified the lack of proficiency in the Japanese language as one characteristic of foreigners that should be considered in disaster prevention *** article seeks to understand how proficiency in a local language affects disaster information gathering behavior by using the results of a questionnaire survey conducted after the 2011 Great East Japan Earthquake. Respondents were categorized based on their Japanese and English language abilities. Their media mode, language preferences, information importance, and information-gathering difficulties also were examined. It was found that foreigners skilled in Japanese demonstrated similar information gathering behavior as Japanese respondents, but foreigners unskilled in Japanese showed little usage of Japanese-language media. This group also encountered difficulties due to a lack of Japanese proficiency, but many members were able to acquire some level of Japanese-language information through Internet-based methods. To address language proficiency in disaster prevention planning, information provision in languages other than Japanese should be increased, and Japanese information should be shared in a way that facilitates translation. Although this survey wassignificant in its scope, the results should be considered within the limitations of the Internet-based response collection and focus only on the less-affected area of Japan.

关键词： 2011 Great East Japan Earthquake Disaster information Foreign population Internet-based media Language proficiency

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