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One-Step Multiplex PCR for Simultaneous Detection and Identification of Eight Medically Important <i>Candida</i>Species

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Open Journal of Stomatology 2021年第1期11卷 14-24页

作者： Akira Fukatsu Osamu Tsuzukibashi hidenori suzuk Katsuhiro Asaka Yoshinori Ono Mana Fuchigami Taira Kobayashi Satoshi Uchibori Yuji Takahashi Chiaki Komine Yoshimi Konishi Yuki Ogura Hiroko Omori Masanobu Wakami Hiroshi Murakami Masahiko Fukumoto Division of Laboratory Medicine for Dentistry Department of Oral Health Science Nihon University School of Dentistry at Matsudo Chiba Japan Laboratory Medicine for Dentistry Nihon University Graduate School of Dentistry at Matsudo Chiba Japan Department of Fixed Prosthodontics and Oral Implantology Nihon University School of Dentistry at Matsudo Chiba Japan Division of Oral Function and Rehabilitation Department of Oral Health Science Nihon University School of Dentistry at Matsudo Chiba Japan

Recently, the incidence of Candida infections has substantially increased. Conventional identification methods for Candida species are technically difficult to conduct and cannot accurately distinguish each species. The purpose of the present study was to design primers to identify and detect simultaneously eight medically important Candida species using one-step multiplex PCR. PCR primers were designed based on partial sequences of intergenic spacer (IGS) and internal transcribed spacer (ITS) genes of eight medically important Candida species. These primers were able to distinguish each Candida species and did not display cross-reactivity with representative Candida species other than the eight Candida species. Moreover, our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and worked without requiring DNA extraction.

关键词： Candida Candida albicans One-Step Multiplex PCR PCR Method

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