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Development of an in vitro protein digestibility assay mimicking the chicken digestive tract

Animal Nutrition 2018年第4期4卷 401-409页

作者： Dervan D.S.l.Bryan Dawn A.Abbott henry l.classen Department of Animal and Poultry Science University of Saskatchewan

It is difficult to obtain in vivo digestion kinetics data of high protein ingredients using chickens. Collecting kinetics data requires repeated sampling of digesta from the small intestine during the digestion process,which is not easily accomplished due to the anatomical structure of chicken digestive tract. An in vitro technique is proposed for measuring the digestion kinetics of protein sources fed to chickens. The method has a 30 min gastric and 3 h intestinal phase. Five hundred milligram crude protein(CP)equivalent of each meal sample(CP = % N × 6.25) was digested with pepsin(28,260 units) in 50 m l polyethylene centrifuge tubes for 30 min in a shaking water bath(150 strokes/min; 30 mm stroke length)at 41C. The 6.5 ml pancreatin was selected as the enzyme concentration for the intestinal phase, during which time 500 m l aliquots were collected at 0, 15, 30, 45, 60, 90, 120, 150, 180 and 240 min. Samples were diluted 1:820 with HCl and sodium acetate buffer, and then mixed with ninhydrin reagent(2:1) at100 ± 2C for 15 min and spectrometric readings taken at 568 nm. To validate the assay, 5 replications of soybean meal(SBM), corn gluten meal(CGM), corn distillers dried grains with solubles(CDDGS), porcine meal(PCM), fish meal(FM) and casein(CA) were digested. The digestion data were modeled with PROC NlIN procedure, and the intra coefficient of variation(CV) assessed using PROC MEANS of SAS 9.4. The digestion values at 180 min were SBM 95 ± 4, FM 93 ± 3, PCM 68 ± 4, CGM 82 ± 3 and CDDGS 70 ± *** CV for SBM, CGM, CDDGS, PCM and FM were 5%, 5%, 12%, 10% and 2%, respectively. The estimated fractional digestion rates for SBM, CGM, CDDGS, FM and PCM were 0.023, 0.013, 0.009, 0.024 and 0.013,respectively. In conclusion, the proposed in vitro technique estimated the rate and extent of the digestion of CP for the meals with low intra CV.

关键词： Protein digestion rate Soybean meal Corn gluten meal Corn distillers dried grains with solubles Fish meal Porcine meal

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Effect of protein sources on performance characteristics of turkeys in the first three weeks of life

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Animal Nutrition 2019年第4期5卷 396-406页

作者： Megan l.Ross Dervan D.S.l.Bryan Dawn A.Abbott henry l.classen Department of Animal and Poultry Science University of SaskatchewanSaskatoonS7N 5A8SaskatchewanCanada

The effect of nutrition during the early life of turkey poults has a long-lasting impact on bird *** study assessed the digestibility of 5 high protein feed ingredients(soybean meal [SBM],corn gluten meal [CGM],canola protein concentrate [CPC],fish meal [FM],and porcine meal [PCM]) in broiler chickens,as well as their use in turkey pre-starter diets fed to 21 d of *** first experiment(5 × 2 factorial arrangement) determined nitrogen corrected apparent metabolizable energy(AMEn) and apparent ileal amino acid digestibility(AIAAD) of each ingredient in broiler chickens at 5 and 21 d of age,using 6 replications of 30 and 8 chicks,*** the second experiment(completely randomized design),4 replication pens,containing 23 d-old poults,were randomly assigned to one of 5 dietary *** diets were formulated based on the AMEn and AIAAD values derived in the first experiment,and consisted of a high SBM control diet,and 4 additional diets with either CPC,FM,PCM or CGM replacing 25% of the protein supplied by SBM in the control *** analysis was completed using Proc Mixed in SAS *** contrasts were used to compare treatments in the second *** were identified at P lue at d 5 was higher than that at d *** ileal amino acid digestibility increased with age for most amino acids(AA),but the response was AA and protein source *** largest average increase in AIAAD between 5 and 21 d of age was observed for *** of CPC,FM,PCM,or CGM increased body weight up to 14 d,in comparison to poults fed the SBM diet,but feed efficiency and water consumption were not *** ileum digesta moisture values were higher for birds fed SBM when compared to those fed *** results demonstrate that combining SBM with CPC,FM,PCM,or CGM improves poult performance during the first 14 d of

关键词： Soybean meal Corn gluten meal Canola meal Fish meal Porcine meal Turkeys

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Diet medication and beta-glucanase affect ileal digesta soluble betaglucan molecular weight,carbohydrate fermentation,and performance of coccidiosis vaccinated broiler chickens given wheatbased diets

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Animal Nutrition 2023年第4期15卷 288-296页

作者： Namalika D.Karunaratne henry l.classen Andrew Gvan Kessel Michael R.Bedford Nancy P.Ames Rex W.Newkirk Department of Animal and Poultry Science University of SaskatchewanSaskatoonSK S7N5A8Canada AB Vista MarlboroughWiltshireSN84ANUnited Kingdom Agriculture and Agri-Food Canada WinnipegR3T 2E1ManitobaCanada

Exogenous enzymes as alternatives to feed antibiotics in poultry has become an emerging research area with the emergence of antibiotic *** objective was to evaluate the effects of diet medication(antibiotics)andβ-glucanase(BGase)on digesta solubleβ-glucan depolymerization,carbohydrate fermentation,and performance of coccidiosis-vaccinated broiler chickens fed wheat-based diets.A total of 1,782 broilers were raised on litter floor pens,and each treatment was assigned to 1 pen in each of the9 *** 3 dietary treatments were based on wheat as the sole grain(control,control+medication and control+0.1%BGase),and the birds were fed the respective treatments ad libitum from 0 to 33 *** were arranged in a randomized complete block design and analyzed as a one-way ***-glucanase reduced the peak molecular weight,weight average molecular weight(Mw)and maximum molecular weight for the smallest 10%β-glucan molecules(MW-10%)in ileal digesta at d 11and 33,whereas diet medication reduced Mw and MW-10%at d 33 compared to the control(Plucanase and medication reduced the ileal viscosity at d 11 compared to the control(P=0.010).Ileal propionic acid concentration at d 11 and caecal total SCFA,acetic,and butyric acid concentrations at d 33 were lower in the BGase-supplemented diet than in the control(Pl p H compared to the control at d 33(P=0.026).The effect of medication on carbohydrate fermentation was *** medication increased weight gain after d 11,whereas BGase increased the gain for the total trial period compared to the control(Pl(Plarger than BGase,considering weight gain and feed efficiency after d 11(Plusion,diet medication and BGase depolymerized high molecular weight ileal solubleβ-g

关键词： Beta-glucan Prebiotic Non-starch polysaccharide Soluble fiber Feed antibiotic

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Hulless barley and β-glucanase affect ileal digesta soluble β-glucan molecular weight and digestive tract characteristics of coccidiosis-vaccinated broilers

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Animal Nutrition 2021年第3期7卷 595-608页

作者： Namalika D.Karunaratne Rex W.Newkirk Nancy P.Ames Andrew G.Van Kessel Michael R.Bedford henry l.classen Department of Animal and Poultry Science University of SaskatchewanSaskatoonS7N 5A8SaskatchewanCanada Agriculture and Agri-food Canada WinnipegR3T 2E1ManitobaCanada AB Vista MarlboroughWiltshireSN84ANUnited Kingdom

Exogenousβ-glucanase(BGase)in barley-based feed has been shown to reduce digesta viscosity in chickens,and thereby improve *** well studied is the potential for BGase to convert barleyβ-glucan into low molecular weight carbohydrates,which might influence digestive tract function and enteric ***-vaccinated broiler chickens were fed graded levels of hulless barley(HB)and BGase to determine their effects onβ-glucan depolymerization and digestive tract *** were fed highβ-glucan HB(0%,30%and 60%replacing wheat)and BGase(0%,0.01%and 0.1%)in a 3×3 factorial arrangement.A total of 5,346 broilers were raised in litter floor pens and vaccinated for coccidiosis on d *** treatment was assigned to 1 pen in each of 9 *** significance level was set at P≤*** both 11 and 33 d of broiler ages,peak molecular weight ofβ-glucan in ileal digesta decreased with increasing BGase for 30%and 60%*** maximum molecular weight for the smallest 10%β-glucan molecules(MW-10%)decreased with BGase at both ages for 30%and 60%HB;for birds fed 0%HB,only 0.1%BGase decreased MW-10%.The 0.1%BGase increased caecal short chain fatty acids(SCFA)compared to the 0.01%BGase at d 11 only for the 60%*** pH increased with increasing HB and BGase at d 11 and *** pH was lower for 0.1%BGase than 0%BGase for 60%HB at d *** mRNA expression of interleukin 6(Il-6)and Il-8 in the ileum increased with 0.1%BGase at d 11 and 33,respectively,whereas expression of ileal mucin 2(MUC2)decreased with 0.1%BGase at d *** the caeca,interactions between HB and BGase were significant for monocarboxylate transporter 1(MCT1)and mucin 5AC(MUC5AC)on d 11,but no treatment effects were found at d *** conclusion,BGase depolymerized high molecular weightβ-glucan in HB in a dose-dependent *** barley and BGase did not increase SCFA concentrations(except for 60%HB with 0.1%BGase at d 11)and caused minor effects on digestive tract histomorphological

关键词： Prebiotic Non-starch polysaccharide Fermentation Short-chain fatty acid Feed enzyme

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Hepatocardiorenal syndrome in liver cirrhosis:Recognition of a new entity?

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World Journal of Gastroenterology 2024年第2期30卷 128-136页

作者： henry H l Wu Amina Rakisheva Arvind Ponnusamy Rajkumar Chinnadurai Renal Research Kolling Institute of Medical ResearchRoyal North Shore Hospital&The University of SydneySt.Leonards(Sydney)2065New South WalesAustralia Department of Cardiology City Cardiological CenterAlmaty 050000Kazakhstan Department of Renal Medicine Royal Preston HospitalPreston PR29HTUnited Kingdom Donal O’Donoghue Renal Research Centre&Department of Renal Medicine Northern Care Alliance National Health Service Foundation TrustSalford M68HDUnited Kingdom

Emerging evidence and perspectives have pointed towards the heart playing an important role in hepatorenal syndrome(HRS),outside of conventional understanding that liver cirrhosis is traditionally considered the sole origin of a cascade of pathophysiological mechanisms directly affecting the kidneys in this *** the absence of established heart disease,cirrhotic cardiomyopathy may occur more frequently in those with liver cirrhosis and kidney *** is a specific form of cardiac dysfunction characterized by blunted contractile responsiveness to stress stimuli and altered diastolic relaxation with electrophysiological *** the clinical description of these potential cardiac-related complications of the liver,the role of the heart has traditionally been an overlooked aspect of circulatory dysfunction in *** from a physiological sense,temporality(prior onset)of cardiorenal interactions in HRS and positive effects stemming from portosystemic shunting demonstrated an important role of the heart in the development and progression of kidney dysfunction in cirrhotic *** this review,we discuss current concepts surrounding how the heart may influence the development and progression of HRS,and the role of systemic inflammation and endothelial dysfunction causing circulatory dysfunction within this *** temporality of heart and kidney dysfunction in HRS will be *** a subgroup of patients who receive portosystemic shunting,the dynamics of cardiorenal interactions following treatment is *** research to determine the unknowns in this topic is anticipated,hopefully to further clarify the intricacies surrounding the liver-heart-kidney connection and improve strategies for management.

关键词： Hepatorenal syndrome Cardiorenal syndrome Cirrhosis Cardiac dysfunction Circulatory dysfunction

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Unveiling strain-enhanced moiréexciton localization in twisted van der Waals homostructures

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Nano Research 2024年第4期17卷 3245-3252页

作者： henry Rui He Haihong Zheng Biao Wu Shaofei li Junnan Ding Zongwen liu Jian-Tao Wang Anlian Pan Yanping liu Institute of Quantum Physics School of PhysicsCentral South UniversityChangsha 410083China State Key laboratory of Precision Manufacturing for Extreme Service Performance Central South UniversityChangsha 410083China School of Chemical and Biomolecular Engineering The University of SydneyNSW 2006Australia Beijing National laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190China College of Materials Science and Engineering Hunan UniversityChangsha 410082China State Key laboratory of High-Performance Complex Manufacturing Central South UniversityChangsha 410083China Shenzhen Research Institute of Central South University Shenzhen 51800China

Moirésuperlattices,arising from the controlled twisting of van der Waals homostructures at specific angles,have emerged as a promising platform for quantum emission ***,the manipulation of strain provides a versatile strategy to finely adjust electronic band structures,enhance exciton luminescence efficiency,and establish a robust foundation for two-dimensional quantum light ***,the intricate interplay between strain and moirépotential remains partially ***,we introduce a meticulously designed fusion of strain engineering and the twisted 2l-WSe_(2)/2l-WSe_(2) homobilayers,resulting in the precise localization of moiré*** low-temperature photoluminescence spectroscopy,we unveil the emergence of highly localized moiré-enhanced emission,characterized by the presence of multiple distinct emission ***,our investigation demonstrates the effective regulation of moirépotential depths through strain engineering,with the potential depths of strained and unstrained regions differing by 91%.By combining both experimental and theoretical approaches,our study elucidates the complex relationship between strain and moirépotential,thereby opening avenues for generating strain-induced moiréexciton single-photon sources.

关键词： moirésuperlattices strain engineering moiréexcitons moirépotential

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Existence of Solutions of a Convolution Integral Equation

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Journal of Applied Mathematics and Physics 2024年第5期12卷 1835-1847页

作者： henry Otoo Benjamin Dadzie Mensah lewis Brew Mathematical Sciences Department University of Mines and Technology Tarkwa Ghana Faculty of Science and Technology University of Silesia in Katowice Sosnowiec Poland

In this study, we prove the of existence of solutions of a convolution Volterra integral equation in the space of the lebesgue integrable function on the set of positive real numbers and with the standard norm defined on it. An operator P was assigned to the convolution integral operator which was later expressed in terms of the superposition operator and the nonlinear operator. Given a ball Br belonging to the space l it was established that the operator P maps the ball into itself. The Hausdorff measure of noncompactness was then applied by first proving that given a set M∈ B r the set is bounded, closed, convex and nondecreasing. Finally, the Darbo fixed point theorem was applied on the measure obtained from the set E belonging to M. From this application, it was observed that the conditions for the Darbo fixed point theorem was satisfied. This indicated the presence of at least a fixed point for the integral equation which thereby implying the existence of solutions for the integral equation.

关键词： Volterra Integral Equation Convolution Fixed Point Existence Noncompactness

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不同微区分析方法在2205不锈钢固液界面偏析中的应用

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热加工工艺 2024年

作者：张洪瑞侯智伦汪国祥 Hu henry 陈湘茹翟启杰上海大学材料科学与工程学院先进凝固技术中心泰州鑫宇精工股份有限公司加拿大温莎大学机械汽车材料系

2205不锈钢作为第二代不锈钢已被广泛应用于耐蚀性高的石油管道、海洋平台等工业领域。定向凝固能够揭示不锈钢的凝固过程，并为研究凝固过程中溶质偏析提供基础。微区分析方法的种类可分为波谱及能谱两类，本文分别以电子探针（EPMA）... 详细信息

2205不锈钢作为第二代不锈钢已被广泛应用于耐蚀性高的石油管道、海洋平台等工业领域。定向凝固能够揭示不锈钢的凝固过程，并为研究凝固过程中溶质偏析提供基础。微区分析方法的种类可分为波谱及能谱两类，本文分别以电子探针（EPMA）配备的波谱仪（WDS）及能谱仪（EDS）对2205不锈钢材料固液界面的溶质偏析进行分析及对比，以研究不同仪器对于微观溶质偏析结果准确性的差异。结果表明，相对于能谱仪，电子探针配备的波谱仪能够更加直观地反映出固液界面元素的变化规律，其测定结果具有更高的精确性。

关键词： 2205不锈钢电子探针能谱波谱元素偏析

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Stress reduction via neuro-emotional technique to achieve the simultaneous resolution of chronic low back pain with multiple inflammatory and biobehavioural indicators:A randomized,double-blinded,placebo-controlled trial

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Journal of Integrative Medicine 2022年第2期20卷 135-144页

作者： Peter Bablis henry Pollard Anthony l.Rosner Universal Health New South Wales 2028Australia School of Medical and Applied Sciences University of Central QueenslandBrisbaneNew South Wales 4000Australia Medical Information Services MA 02479-4149United States

Background:Beginning with the concepts of stress developed by Selye,an approach to stress and pain management,known as neuro-emotional technique(NET),has been *** is a treatment approach based on the principle that the stressor effects of dormant and/or current unresolved issues or trauma are what determine one’s bodily *** responses are relatively personalized to the conditioned,experiential and emotional reality of the ***:To determine the effect of NET on patients with chronic low back pain(ClBP)over ***,setting,participants,and interventions:In a randomized,double-blinded,placebo-controlled study conducted in a single clinic,NET or control treatments were given twice weekly for 4 weeks in a population of 112 *** outcome measures:Outcome measures,including Oswestry Disability Index,Quadruple Visual Analogue Scale,the psychoneuroimmunology markers of blood serum levels of C-reactive protein,tumour necrosis factor-a,interleukin-1(Il-1),Il-6,and Il-10,and 10 dimensions of the Short Form Health Survey scale,were assessed at baseline and at 1,3 and 6 months following the intervention ***:Compared to placebo,NET produced clinical and statistical significance(Plines of virtually all physiological,pain and disability markers,accompanied by gains in quality-of-life indicators at 0(baseline),1,3 and 6 *** of the percentages of patients whose 5 biomarkers lay outside the normative range were achieved at 1,3 and 6 months by NET but not control ***:A randomized,controlled trial of ClBP patients indicated that 8 NET interventions,compared to placebo,produced clinically and statistically significant reductions in pain,disability and inflammatory biomarkers,and improvements in quality-of-life measures.

关键词： Neuro-emotional technique Biopsychosocial model Emotion Mind-body therapies Inflammation Biomarker

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Microenvironmental engineering:An effective strategy for tailoring enzymatic activities

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Chinese Journal of Chemical Engineering 2020年第8期28卷 2028-2036页

作者： Yifei Zhang henry Hess Department of Biomedical Engineering Columbia University351L Engineering Terrace1210 Amsterdam AvenueNew YorkNY 10027USA

Rationally,engineering a favorable physicochemical microenvironment for enzymes has recently emerged as an effective strategy to improve their catalytic *** this review,we discuss four microenvironmental effects according to the mechanism of action:localizing and excluding reactants and regulators,regulating microenvironmental pH,creating a water-like microenvironment,and increasing the local *** mechanisms are enzyme-independent and can in principle be used in combination to tailor enzyme behaviors,offering new approaches to enabling,enhancing,and regulating enzyme catalysis in diverse applications without the need for genetic engineering.

关键词： Biocatalysis Enzyme Microenvironment Activity enhancement

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