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Coating independent cytotoxicity of citrate-and PEG-coated silver nanoparticles on a human hepatoma cell line

Journal of Environmental Sciences 2017年第1期29卷 191-201页

作者： Veronica Bastos Jose M.P.Ferreira-de-oliveira Joana Carrola Ana L.Daniel-da-Silva Iola F.Duarte Conceicao Santos helena oliveira CESAM&Laboratory of Biotechnology and Cytomics Department of BiologyUniversity of Aveiro3810-193 AveiroPortugal CICECO-Aveiro Institute of Materials Department of ChemistryUniversity of Aveiro3810-193 AveiroPortugal Department of Biology Faculty of SciencesUniversity of Porto4169-007 PortoPortugal

The antibacterial potential of silver nanoparticles（AgNPs） resulted in their increasing incorporation into consumer,industrial and biomedical ***,human and environmental exposure to AgNPs（either as an engineered product or a contaminant）supports the emergent research on the features conferring them different toxicity *** this study,30 ran AgNPs coated with citrate or poly（ethylene glycol）（PEG） were used to assess the influence of coating on the effects produced on a human hepatoma cell line（HepG2）,namely in terms of viability,apoptosis,apoptotic related genes,cell cycle and cyclins gene *** types of coated AgNPs decreased cell proliferation and viability with a similar toxicity *** the concentrations used（11 and 5 μg/mL corresponding to IC50 and-IC10 levels,respectively） the amount of cells undergoing apoptosis was not significant and the apoptotic related genes BCL2（anti-apoptotic gene）and BAX（pro-apoptotic gene） were both ***,both AgNPs affected HepG2 cell cycle progression at the higher concentration（11 μg/mL） by increasing the percentage of cells in S（synthesis phase） and G2（Gap 2 phase） *** the cell-cycle related genes,the expression of cyclin B1 and cyclin E1 genes were ***,this work has shown that citrate- and PEG-coated AgNPs impact on HepG2 apoptotic gene expression,cell cycle dynamics and cyclin regulation in a similar *** research is needed to determine the properties that confer AgNPs at lower toxicity,since their use has proved helpful in several industrial and biomedical contexts.

关键词： Silver nanoparticles(AgNPs) Citrate Polyethylene glycol)(PEG) HepG2 cells Cytotoxicity Nanotoxicology

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In vitro toxicity of particulate matter emissions from residential pellet combustion

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Journal of Environmental Sciences 2022年第5期34卷 215-226页

作者： Estela D.Vicente Daniela Figueiredo Catia Goncalves Isabel Lopes helena oliveira Nora Kovats Teresa Pinheiro Celia A.Alves Department of Environment and Planning and CESAM-Centre for Environmental and Marine Studies University of Aveiro3810-193 AveiroPortugal Department of Biology and CESAM-Centre for Environmental and Marine Studies University of Aveiro3810-193 AveiroPortugal Centre of Natural Environmental Sciences University of PannoniaEgyetem str.108200 VeszpremHungary Instituto de Bioengenharia e Biociencias Departamento de Engenharia e Ciencias NuclearesInstituto Superior TecnicoUniversidade de LisboaAv.Rovisco Pais 11049-001 LisboaPortugal

Particulate matter emissions(PM10)from the combustion,in a residential stove,of two com-mercial brands of certified(ENplus A1)pellets,a non-certified brand and laboratory made pellets of acacia were tested for their ability to induce ecotoxic,cytotoxic,and mutagenic re-sponses in unicellular organisms and a human cell *** was evaluated through the Vibrio fischeri bioluminescence inhibition ***,cytotoxicity was assessed at two time points(24-and 48-hr)through two complementary techniques in order to evaluate the cellular metabolic activity and membrane integrity of human lung epithelial cells *** Ames test using two Salmonella typhimurium strains(TA100 and TA98)was employed to assess the mutagenic potential of the polycyclic aromatic hydrocarbon fraction extracted from the PM10 *** obtained with the bioluminescent bacteria indicated that only particles from the combustion of acacia pellets were *** samples induced im-pairment on the A549 cells metabolic activity,while no significant release of lactate dehy-drogenase was ***10 emissions from acacia pellets were the most cytotoxic,while samples from both certified pellets evoked significant cytotoxicity at lower ***-icity time-dependency was only observed for PM10 from the combustion of acacia pellets and one of the brands of certified *** activity was not detected in both *** murium *** study emphasises the role of the raw material for pellet manu-facturing on the toxicological profile of PM *** raw materials should be deeply investigated before their use in pelletisation and combustion in residential appli-ances.

关键词： Ecotoxicity Cytotoxicity Mutagenicity Fuel quality Pellet combustion PM10 emissions

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Anaerobic Digestion of Food Waste Using UASB Sludge from a Poultry Slaughterhouse as Inoculum

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Journal of Agricultural Science and Technology(A) 2017年第7期7卷 507-512页

作者： Bruna Patricia de oliveira Luisa helena dos Santos oliveira Gilberto Martins Programa de Pos Graduacao em Energia Universidade Federal do ABC Santo Andre 09210-580 Brazil Programa de Pos Graduacao em Ciencia e Tecnologia Ambiental Universidade Federal do ABC Santo Andre 09210-580 Brazil

In 2010, Brazil endorsed a new national policy of solid wastes, which has a very ambitious target to close up all the dumping areas and to manage adequately all the municipal solid waste, disposing in landfills only the refuse of treated municipal solid waste after 2019. Food waste is the largest representative of organic waste produced in Brazilian municipalities, which can produce both fertilizer and biogas through anaerobic digestion （AD） process, reducing in both cases greenhouse gases emissions. AD assays of some samples of restaurant food wastes were performed in batch laboratory, 500 mL reactors for determination of optimized start-up conditions. The tests were conducted in triplicate, using anaerobic sludge from a poultry slaughterhouse wastewater treatment as inoculum to start up the anaerobic biodigestors. The effect of three different substrates to inoculum mixing ratios 10：90 （S1）, 20：80 （S2） and 30：70 （S3） was evaluated. The food waste used as substrate was previously homogenized and submitted to aerobic mechanical mixing for four days before it was fed in the reactors in anaerobic conditions at the mentioned proportions with the inoculum. The reactors were maintained at 35 ℃ and under mechanical mixing at 60 rpm. The methane production was monitored until its stabilization. Results showed that S1 presented the best performance during the initial 12 d, when it totalized 526 NmLCH4/gVSsubstrate.

关键词： Biogas methane food waste inoculum poultry sludge anaerobic biodigestion.

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Intervention in the Loneliness of the Elderly What Strategies, Challenges and Rewards？

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Journal of Health Science 2015年第2期3卷 71-80页

作者： Manuela Dias Carla Silva Ricardo Dias helena oliveira Primary Care Unit of Terceira Island-Angra do Herolsmo Health Center Angra do Heroismo Terceira Azores 9701-869 Portugal Terceira Island Hospital Angra do Herolsmo Terceira Azores 9700-049 Portugal University of the Azores College of Nursing S. Miguel Azores 9504-538 Portugal

The new challenges that the phenomenon of demographic aging imposes on society in general, and health services in particular, can impact active and healthy aging. The project was implemented for two years （2012/2013） and aimed to combat loneliness in the elderly, which constitutes an obstacle to healthy and active aging. Decrease the sense of loneliness of individuals from 65 to 84 years of age, the project was made by using the health planning methodology, starting with a descriptive study （health diagnosis of the independent elderly population） followed by the development, implementation and evaluation of a community intervention project, which included home visits to the elderly, assessment of the level of loneliness in two different times （UCLA scale） and various intergenerational activities of social life （dancing, walking, online courses, games...） using a cross-section of partnerships and community involvement. There was a decrease from 62.8% to 41.9% in the reported overall feeling of loneliness of independent individuals between the ages of 65 and 84 years of age. There was an increase from 50.0% to 86.4% of seniors who now have dreams/life projects. The reduction of social and emotional isolation resulted in the promotion of social networks, which not only encouraged social interaction but also considered the elderly who live alone. The health gains arising from it are evidenced in contributing more proactively and the improvement of the quality of life in the elderly community.

关键词： Elderly health planning loneliness active aging

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The Development of Mathematical Modelling Activities from Thematic Projects in the Social-Critical Perspective

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Journal of Mathematics and System Science 2012年第6期2卷 341-347页

作者： Regina helena de oliveira Lino Franchi Frederico da Silva Reis Department of Mathematics Federal University of Ouro Preto Ouro Preto 35400-000 Brazil

In this paper, the authors present a modelling activity developed from a thematic project in the models and mathematical modelling class of the professional master＇s degree program in mathematics education at the Federal University of Ouro Preto. The students enrolled in the class chose to study the theme Brazilian Public Politics. After several debates regarding the theme, the students were separated into three groups and each group chose a subtheme to study. The themes were IDH （index of human development）, minimum wage and social security. As an example, a description of the thematic project about the minimum wage in Brazil is presented. The principal results obtained in the study of this theme include a discussion about the potentialities of the theme as a classroom activity in different levels of schooling. As an educational activity, the thematic project demonstrated itself to be greatly relevant, since not only did it provide the learning possibility of mathematics related to the issues studied, but also promoted various discussions regarding the theme, which, in our conception, contributes to the social-critical development of the students. Finally, from the point of view of applied mathematics and mathematics education, the development of a modelling activity from thematic projects showed itself to be a good source of researches.

关键词： Mathematical modelling thematic projects social-critical perspective.

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Agave sisalana extract induces cell death in Aedes aegypti hemocytes increasing nitric oxide production

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Asian Pacific Journal of Tropical Biomedicine 2016年第5期6卷 396-399页

作者： Louise helena Guimaraes de oliveira Patricia Alexandria Paiva Silva de Sousa Fabrine Felipe Hilario Gabriel Joventino Nascimento Joao Paulo Saraiva Morais Everaldo Paulo de Medeiros Manoel Francisco de Sousa Fabiola da Cruz Nunes Biotechnology Center Federal University of Paraiba Embrapa Algodao Brazilian Agricultural Research Corporation

Objective: To investigate the effects of Agave sisalana(A. sisalana) extract on Aedes aegypti(Ae. aegypti) primary cell ***: Cells of Ae. aegypti were exposed to different concentrations of A. sisalana crude extract(0.18–6.00 mg/m L) for 24 h. Then, the cells were labeled with propidium iodide and subjected to fluorescence microscopy to verify cell viability. In addition, nitric oxide production was ***: Results showed that cells exposed to 6 mg/m L of the crude extract presented a greater percentage of death when compared to control(73.8% ± 9.6% vs. 34.6% ± 9.6%).Furthermore, there was an increase in the nitric oxide production in cells exposed to 6 mg/m L of A. sisalana crude extract [(0.81 ± 0.08) mmol/L] compared to control group[(0.41 ± 0.18) mmol/L].Conclusions: The results show that A. sisalana is cytotoxic to Ae. aegypti and may be used as raw material for new eco-friendly and inexpensive insecticides, since sisal industry discards the liquid waste for the extraction of plant fiber.

关键词： Sisal Cell culture Mosquito Insecticide

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巴西东南部夏、冬季吹蝇幼虫的食后扩散(英文)

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动物学报 2008年第1期54卷 176-182页

作者： Leonardo GOMES Guilherme GOMES helena Gutierrez oliveira Margareth Maria de Carvalho QUEIROZ Claudio José Von ZUBEN Eco-epidemiological Laboratory of Chagas Decease Instituto Oswaldo Cruz-IOC/FIOCRUZ room A10 Brasil Avenue 4365 Manguinhos PO BOX 926 21045-900 Rio de Janeiro RJ Brazil Department of Zoology IB UNESP Bairro Bela Vista 13506-900 Rio Claro SP Brazil

估计死亡和案发之间的最短时间是法医昆虫学的重要目标之一。在估计死亡时间时,研究吹蝇扩散至关重要。如果低估了最早扩散的幼虫距离和深度就可能低估死亡的时间。因此,掌握影响吹蝇幼虫扩散的有关因素,包括竞争、同类相食、在食源上... 详细信息

估计死亡和案发之间的最短时间是法医昆虫学的重要目标之一。在估计死亡时间时,研究吹蝇扩散至关重要。如果低估了最早扩散的幼虫距离和深度就可能低估死亡的时间。因此,掌握影响吹蝇幼虫扩散的有关因素,包括竞争、同类相食、在食源上的聚集密度、光周期、温湿度等以及潜入习性,进而推测吹蝇幼虫在尸体附近或远离尸体化蛹等都会影响估计死亡时间的准确性。本研究分析了从2006年夏季至2007年冬季在巴西南部自然状态下吹蝇食后的扩散。除趋光性外,扩散距离在季节上的不同(夏季3·1 m对应冬季的1·2 m)是由于环境条件起了重要作用。在用屠宰动物模拟实验时显示,吹蝇幼虫在低温低湿环境下扩散的时间滞后,扩散的距离也缩短。本研究表明温度(也许包括湿度)影响吹蝇幼虫的扩散,环境条件也决定吹蝇何时开始扩散。

关键词：吹蝇双翅目法医昆虫学幼虫扩散

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Impact of solar reflectivity and infrared emissivity on the thermal performance of metal and concrete roofs in cloudy warmhumid climate

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Frontiers of Architectural Research 2024年第4期13卷 842-857页

作者： Jefferson Torres-Quezada Antonio Isalgué helena Coch CONSISO Lab Universidad Católica de CuencaCuenca 010102Ecuador Architecture Energy and EnvironmentPolytechnic University of CataloniaBarcelona 08028Spain

Regions near latitude 0are characterized by warm-humid climate and also by high *** these regions,metal roofs has been the most widely used ***,in the last decades,the use of heavy concrete roofs has increased *** its material characteristics,this roof typology offers a higher thermal resistance and thermal mass than a metal *** strategies focus on the use of these characteristics,as well as the use of high reflectivity and ***,the impact of cloudiness on the effectiveness of these strategies has been little *** research focuses on the impact of reflectivity and emissivity change on the thermal performance of these two roofs in a cloudy warm-humid *** achieve this objective,simulations validated with measurements were *** results show that the efficiency of reflectivity and emissivity is lower in this region compared to other *** impact of these properties is further reduced with increasing thermal mass or decreasing thermal transmittance,so the effectiveness of reflectivity and emissivity is minimal on the concrete ***,this study supports that a metal roof with a reflectivity and emissivity above 0.70 can offer lower daily average temperatures than a concrete roof.

关键词： Concrete roof Metal roof Radiative properties Simulations Measurements

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Making the case for the evolving role of social media in health professions education and a literature review on the application of some in ophthalmology

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Annals of Eye Science 2024年第2期9卷 1-11页

作者： helena Prior Filipe Mila Kostic Mildred Lopez Karl Golnik West Lisbon Hospitals Center Hospital Egas MonizLisbonPortugal Egas Moniz Center for Interdisciplinary Research LisbonPortugal Continuing Education and Development in Health Professions RichboroPAUSA Coordinación de Educación en Salud Instituto Mexicano del Seguro Social(IMSS)Mexico CityMexico Department of Neurology Barrow Neurological InstitutePhoenixAZUSA Department of Ophthalmology University of CincinnatiCincinnatiOHUSA

Background and Objective:Social media(SoMe)has emerged as a tool in health professions education(HPE),particularly amidst the challenges posed by the coronavirus disease 2019(COVID-19)*** the academia’s initial skepticism SoMe has been gaining traction in supporting learning communities,and offering opportunities for innovation in *** study aims to explore the integration of SoMe in *** key components were outlined as necessary for a successful integration,and include designing learning experiences,defining educator roles,selecting appropriate platforms,and establishing educational ***:This article stemmed from the online Teaching Skills Series module on SoMe in education from the Ophthalmology Foundation,and drew upon evidence supporting learning theories relevant to SoMe integration and models of ***,we conducted a literature review considering Englishlanguage articles on the application of SoMe in ophthalmology from PubMed over the past *** Content and Findings:Early adopters of SoMe platforms in HPE have leveraged these tools to enhance learning experiences through interaction,dialogue,content sharing,and active learning *** integrating SoMe into educational programs,both online and in-person,educators can overcome time and geographical constraints,fostering more diverse and inclusive learning *** consideration is,however,necessary to address potential limitations within ***:This article lays groundwork for expanding SoMe integration in HPE design,emphasizing the supportive scaffold of various learning theories,and the need of furthering robust research on examining its advantages over traditional educational *** literature review underscores an ongoing multifaceted,random application of SoMe platforms in ophthalmology *** advocate for an effective incorporation of SoMe in HPE education,with the need to comply with good educational practice.

关键词： Social media(SoMe) health professions education(HPE) active learning social learning technology enhanced learning

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Contributions of World Literatures in Portuguese to the Academic Field

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Journal of Foreign Languages and Cultures 2024年第1期8卷 48-54页

作者： helena BUESCU University of Lisbon

As one considers the concept of comparative world literature,one may ponder on how widening the perspective from an all-English area of studies to other languages promotes different worldviews and descriptions of the status *** this article,we take into consideration the perspective of literature written in Portuguese,be it European,Brazilian,African of even Asian,in order to demonstrate how rich such other points of view are for the *** also engage the concept of defamiliarization(ostranenie),proposed by Russian Formalist,Viktor Shklovsky,as a central tool to consider cosmopolitanism and the dialogue between different literatures.

关键词： comparative world literature literatures in Portuguese defamiliarization(ostranenie) textualism

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