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发作和癫痫分类框架术语及概念的修订：国际抗癫痫联盟分类和术语委员会报告，2005—2009

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中华神经科杂志 2011年第3期44卷 214-216页

作者： Anne T.Berg Samuel F.Berkovie Martin J.Bordie Jeffrey Buchhaher J.helen cross Walter van Emde Boas Jerome Engel Jacqueline French Traey A.Glauser Gray W.Mathern Solomon L.Moshe Douglas Nordli Perrine Plouin Ingrid E.Scheffer 广东省人民医院神经科广州医学院第二附属医院神经内科广州医学院神经科学研究所

癫痫分类的历史很大程度上都是取决于敏锐的临床观察和专家意见。首次对发作的分类始于1960年，随后官方做出的更新是1981年发作分类[1981年国际抗癫痫联盟（ILAE）分类和术语委员会]和1989年的癫痫分类（1989年ILAE分类和术语委员会）。

关键词：国际抗癫痫联盟委员会分类发作修订专家意见临床观察

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儿童持续性部分性癫痫的诊断算法

癫痫杂志 2021年第3期7卷 265-272页

作者： Snehal Surana Thomas Rossor Yael Hacohen Hassell J Boyd S D’Arco F Aylett S Bhate S Carr L Das K DeVile C Eltze C Hemingway C Kaliakatsos M O’Callaghan F Prabhakar P Robinson R Varadkar S helen cross J Hacohen Y 陈晨(译) 慕洁(审) 不详

总结一组持续性部分性癫痫(Epilepsia partialis continua,EPC)患儿的特点,并提出一种整合关键鉴别诊断的诊断算法。对2002年—2019年在三级儿科神经病学中心就诊的EPC患儿进行分析。54例患儿符合EPC诊断标准。中位发病年龄为7岁(0.6~15... 详细信息

总结一组持续性部分性癫痫(Epilepsia partialis continua,EPC)患儿的特点,并提出一种整合关键鉴别诊断的诊断算法。对2002年—2019年在三级儿科神经病学中心就诊的EPC患儿进行分析。54例患儿符合EPC诊断标准。中位发病年龄为7岁(0.6~15岁),中位随访时间为4.3年(0.2~16年)。其中,诊断为Rasmussen脑炎(Rasmussen encephalitis,RE)30例(56%),线粒体疾病12例(22.2%),磁共振成像(MRI)阳性病灶相关局灶性癫痫6例(11.1%),诊断不明5例(9%)。线粒体疾病患儿更早出现EPC,EPC发病年龄每增加一年,诊断线粒体疾病的几率降低26%(P=0.02)。EPC发作前存在发育问题(OR=22,P<0.001),EPC发作前无癫痫发作(OR=22,P<0.001), EEG提示双侧半球慢波(OR 26,P<0.001),以及脑脊液(CSF)蛋白水平升高(OR=16)均预示着线粒体疾病。30例RE患儿中有18例(60%)出现EPC发作时,MRI提示明显不对称或一侧半球萎缩,其余患儿出现的中位时间则在EPC发作后6个月(3~15个月)。首选诊断检查为头部MRI。一侧半球萎缩结合临床表现及脑电图改变的偏侧性,提示诊断为RE。对于初次扫描时不能诊断为RE,但临床和影像学表现提示RE的儿童,建议每6个月重复影像学检查以排查进行性一侧半球皮质萎缩,并应考虑脑活检,鞘内炎症的证据(寡克隆带和新蝶呤升高)可为佐证。对于存在双侧半球源性EPC的患儿,建议进行快速DNA聚合酶γ(POLG)基因检测,如为阴性,则应提取外周血DNA进行mtDNA测序及全外显子组测序。线粒体病所致EPC患儿表现出与RE和结构性癫痫不同的临床特征。这一针对EPC患儿的诊断算法有助于进行针对性检查并及时诊断。

关键词：自身免疫性癫痫持续性部分性癫痫线粒体疾病 POLG相关癫痫 Rasmussen脑炎

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Costimulation of resting B lymphocytes alters the IL-4-activated IRS2 signaling pathway in a STAT6 independent manner: implications for cell survival and proliferation

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Cell Research 2001年第1期11卷 44-54页

作者： ZAMORANO JOSE,Unidad de Investigacion, Hospital San Pedro de Alcantara, Avda Millan Astray, 10003 Caceres ANN E KELLY, JONATHAN AUSTRIAN, helen Y WANG, ACHSAH D KEEGAN (Department of Immunology, Jerome Holland Labs, American Red cross, Rockville, MD, USA Department of Immunology Jerome Holland Labs American Red Cross Rockville USA Unidad de Investigacion Hospital San Pedro de Alcantala Avda Millan Astray Caceres

IL-4 is an important B cell survival and growth factor. IL-4 induced the tyrosine phosphorylation of IRS2 in resting B lymphocytes and in LPS- or CD40L-activated blasts. Phosphorylated IRS2 coprecipitated with the p85 subunit of PI 3’ kinase in both resting and activated cells. By contrast, association of phosphorylated IRS2 with GRB2 was not detected in resting B cells after IL-4 treatment although both proteins were expressed. However, IL-4 induced association of IRS2 with GRB2 in B cell blasts. The pattern of IL-4- induced recruitment of p85 and GRB2 to IRS2 observed in B cells derived from STAT6 null mice was identical to that observed for normal mice. While IL-4 alone does not induce activation of MEK, a MEKI inhibitor suppressed the IL-4-induced proliferative response of LPS-activated B cell blasts. These results demonstrate that costimulation of splenic B cells alters IL-4-induced signal transduction independent of STAT6 leading to proliferation. Furthermore, proliferation induced by IL-4 in LPS-activated blasts is dependent upon the MAP kinase pathway.

关键词： B lymphocytes IL-4 survival proliferation.

Analysis of Natural Allelic Variation Controlling Arabidopsis thaliana Seed Germinability in Response to Cold and Dark： Identification of Three Major Quantitative Trait Loci

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Molecular Plant 2008年第1期1卷 145-154页

作者： Ping-Hong Meng Audrey Macquet Olivier Loudet Annie Marion-Poll helen M. North Laboratoire de Biologie des Semences UMR 204 INRA AgroParisTech Institut Jean-Pierre Bourgin F-78026 Versailles Cedex France Station de Genetique et Amelioration des Plantes INRA Institut Jean-Pierre Bourgin F-78026 Versailles Cedex France Present address: Institute of Horticulture Guizhou Academy of Agricultural Sciences GuiYang Guizhou Province 550006 RR. China

Light and temperature are key external factors in the control of Arabidopsis thaliana seed germination and dormancy mechanisms. Perception and response to these stimuli have to ensure that seedling emergence and growth occur at the most advantageous time for correct establishment. Analysis of over 300 Arabidopsis accessions identified 14, from 12 different geographical locations, that were able to germinate to greater than 20% at 6℃ in the dark. This natural variation was exploited to identify genetic loci responsible for cold-tolerant, dark germination. A quantitative trait loci approach was used on recombinant inbred line progeny of a cross between Bay-0 and Shahdara. Six distinct quantitative trait loci were identified, three of which were major loci, each responsible for 17-25% of the phenotypic variability in this trait. Parental phenotypes indicated that the majority of the cold-tolerant, dark-germination characteristics are related to light responses. Validation of the three major loci using heterogeneous inbred families confirmed the feasibility of fine mapping and cloning the genes at the quantitative trait loci responsible for cold-tolerant, dark germination.

关键词： Three Major Quantitative Cold and Dark Natural Allelic Variation

Soil-transmitted helminths and plasmodium falciparum malaria among individuals living in different agroecosystems in two rural communities in the mount Cameroon area:a cross-sectional study

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Infectious Diseases of Poverty 2017年第1期6卷 573-587页

作者： Irene Ule Ngole Sumbele Gladys Belanka Nkemnji helen Kuokuo Kimbi Department of Zoology and Animal Physiology University of BueaBueaCameroon Department of Medical Laboratory Sciences University of BamendaBamendaCameroon

Background:Soil-transmitted helminths(STHs)and Plasmodium falciparum infections remain public health problems in Cameroon.A cross-sectional study was carried out in the Mount Cameroon area to determine the prevalence and intensity of STHs and *** infections in individuals living in different agroecosystems;to assess the influence of these infections on haematological parameters;and to identify the risk factors associated with STH ***:STH and malaria parasites were detected using the Kato-Katz method and Giemsa staining of blood films,*** blood count values were obtained using an automatic haematology *** samples were analysed using the sucrose floatation sedimentation *** and continuous variables were compared as required and logistic regression models were used to assess the risk factors for STH infections and ***:Of the 450 participants examined,STHs,*** and mixed co-infections were detected in 14.0,33.3 and 5.6%of participants,*** higher prevalences of Ascaris(18.8%)and Trichuris(7.9%)infections were observed in participants from tea plantation areas compared to those from banana and palm plantation areas,with similar trends in egg *** prevalence and parasite density were comparable between the different *** overall prevalence of anaemia was 64.2%.The prevalence of haematological manifestations such as moderate(48.0%)and severe(8.0%)anaemia,leucopenia(26.9%)and microcytosis(30.8%)was significantly higher among Plasmodium-STH co-infected *** samples from plantations showed the highest prevalences of STH eggs compared to soil samples from areas around pit toilets and public water *** in a tea plantation area(OR=3.07),age(AOR=1.49)and lack of access to potable water(OR=2.25)were identified as risk factors for STH infections,while the age groups 15-25 years(OR=2.928)and 26-35 years(OR=2.832),and being fem

关键词： Soil-transmitted helminths Plasmodium falciparum malaria Co-infection Anaemia Agroecosystem Haematological values Environmental contamination Cameroon

Symptom clusters and quality of life among patients with advanced heart failure

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Journal of Geriatric Cardiology 2016年第5期13卷 408-414页

作者： Doris SF Yu helen YL Chan Doris YP Leung Elsie Hui Janet WH Sit The Nethersole School of Nursing The Chinese University of Hong Kong Hong Kong China Department of Medicine and Geriatrics Shatin Hospital Hong Kong China

Objectives To identify symptom clusters among patients with advanced heart failure （HF） and the independent relationships with their quality of life （QoL）. Methods This is the secondary data analysis of a cross-sectional study which interviewed 119 patients with advanced HF in the geriatric unit of a regional hospital in Hong Kong. The symptom profile and QoL were assessed by using the Edmonton Symptom Assessment Scale （ESAS） and the McGill QoL Questionnaire. Exploratory factor analysis was used to identify the symptom clusters. Hier- archical regression analysis was used to examine the independent relationships with their QoL, after adjusting the effects of age, gender, and comorbidities. Results The patients were at an advanced age （82.9± 6.5 years）. Three distinct symptom clusters were identified： they were the distress cluster （including shortness of breath, anxiety, and depression）, the decondition cluster （fatigue, drowsiness, nausea, and reduced appetite）, and the discomfort cluster （pain, and sense of generalized discomfort）. These three symptom clusters accounted for 63.25% of variance of the patients＇ symptom experience. The small to moderate correlations between these symptom clusters indicated that they were rather independent of one another. After adjusting the age, gender and comorbidities, the distress （β = -0.635, P 〈 0.001）, the decondition （β = -0.148, P = 0.01）, and the discomfort （β = -0.258, P 〈 0.001） symptom clusters independently predicted their QoL. Conclusions This study identified the distinctive symptom clusters among patients with advanced HF. The results shed light on the need to develop palliative care interventions for optimizing the symptom control for this life-limiting disease.

关键词： Advanced heart failure Palliative care model Quality of life Symptom clusters

Relationship between left-behind status and cognitive function in older Chinese adults: a prospective 3-year cohort study

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General Psychiatry 2023年第3期36卷 148-157页

作者： Hong-Guang Zhang Fang Fan Bao-Liang Zhong helen Fung-Kum Chiu Department of Psychiatry Affiliated Wuhan Mental Health CenterTongji Medical College of Huazhong Universityof Science&TechnologyWuhanHubeiChina Department of Psychiatry Wuhan Mental Health CenterWuhanHubeiChina Department of Clinical Psychology Wuhan Hospital for PsychotherapyWuhanHubeiChina Department of Psychiatry The Chinese University of Hong KongHong KongChina

Background Due to the inaccuracy of the traditional geographical distance-based definition of left-behind status,data on the negative effect of left-behind status on cognitive function among older adults are *** This study examined the cross-sectional and longitudinal associations of left-behind status with cognitive function in older Chinese *** left-behind status definition was based on the frequency of face-to-face parent-child *** Data from a nationally representative sample of 8682 older adults(60+years)in 2015(5658 left behind and 3024 non-left behind),of which 6933 completed the follow-up in 2018,were obtained from the China Health and Retirement Longitudinal ***-behind older adults werebroadlydefined as those aged 60+years who had living adult children and saw their children less than once per *** cognitive function was assessed with a composite cognitive test with higher total scores indicating better cognitive *** Left-behind older adults had significantly lower cognitive test scores than non-left-behind older adults in both 2015(11.1(6.0)vs 13.2(5.9),t=15.863,p<0.001)and 2018(10.0(6.6)vs 12.4(6.7),t=14.177,p<0.001).After adjusting for demographic factors,lifestyle factors,chronic medical conditions and the baseline cognitive test score(in the longitudinal analysis only),on average,the cognitive test score of left-behind older adults was 0.628 lower than their non-left-behind counterparts in 2015(t=5.689,p<0.001).This difference in cognitive test scores attenuated to 0.322 but remained significant in 2018(t=2.733,p=0.006).Conclusions Left-behind older Chinese adults have a higher risk of poor cognitive function and cognitive decline than their non-left-behind *** efforts targeting left-behind older adults,such as encouraging adult children to visit their parents more regularly,are warranted to maintain or delay the progression of cognitive decline.

关键词： function cognitive maintain

An Epigenetic Role for Disrupted Paternal Gene Expression in Postzygotic Seed Abortion in Arabidopsis Interspecific Hybrids

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Molecular Plant 2015年第12期8卷 1766-1775页

作者： Ryan C. Kirkbride helen Hong Yu Gyoungju Nah Changqing Zhang Xiaoli Shi Z. Jeffrey Chen Department of Molecular Biosciences Center for Computational Biology and Bioinformatics and Institute for Cellular and Molecular Biology The University of Texas at Austin Austin TX 78712 USA These authors contributed equally to this article. present address: Department of Plant Science Research Institute of Agriculture and Life Sciences Seoul National University Seou1151-921 South Korea present address: Plants for Human Health Institute North Carolina State University 600 Laureate Way Kannapolis NC 28081 USA present address: Institute of Theoretical Physics Chinese Academy of Sciences PO Box 2735 Beijing 100080 China

Interspecific hybrids often increase the levels of heterozygosity and hybrid vigor, but some interspecific hybrid seeds are aborted shortly after fertilization. The mechanism behind this postzygotic seed abortion is poorly understood. Here, we report genome-wide analysis of allelic expression changes in developing siliques and seeds in three F1 interspecific crosses between Arabidopsis thaliana （Col, Ler, or C24） and Arabidopsis arenosa. The majority of maternally expressed genes （MEGs） were shared among all three F1 interspecific crosses, whereas ~90% of 272 paternally expressed genes （PEGs） were found only in one or two F1 crosses, suggesting a role for disrupted paternal gene expression in seed abortion that varies in different crosses. Consistent with this notion, 12 PEGs in the infertile interspecific hybrids matched MEGs in fertile intraspecific hybrids. This disruption of PEGs in the interspecific hybrids was consistent with the upregulation of the genes in the paternal-excess interploidy cross （2X6） between a diploid mother and a hexaploid father, leading to the seed abortion. Moreover, a subset of PEGs in the interspecific crosses were also upregulated in the intraspecific hybrid metlXWT or meaXWT, in which the mutant of MET1 （DNA METHYL TRANSFERASE1） or MEDEA, a Polycomb Repressive Complex2 gene, was used as the maternal parent. These data suggest that maternal epigenetic factors and paternal gene expression play important roles in the postzygotic seed abortion in interspecific hybrids or neo-allopolyploids.

关键词： epigenetics imprinting paternal gene expression polyploidy hybrid incompatibility seeddevelopment

The Response of Cockerel Semen Kinematic Parameters LIN, STR, WOB, ALH and BCF to Induced Oxidative Stress

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Open Journal of Animal Sciences 2021年第2期11卷 292-303页

作者： Cyril Mpho Pilane Mohleko helen Mapeka Agricultural Research Council Animal Production Campus Germplasm Conservation & Reproductive Biotechnologies Irene South Africa Department of Animal Production and Agricultural Economics Faculty of Science and Agriculture University of Limpopo Sovenga South Africa

Computer-assisted sperm class analyser (CASA) analysis of avian semen following cryopreservation indicates that their semen motility and viability parameters become compromised, due in part to oxidative stress. To mimic these observations we have treated cockerel semen with an oxidative stress inducing agent, namely hydrogen peroxide (H2O2) and monitored the motility, kinematic and viability parameters over time. Briefly, five healthy and fertile South African Venda cockerels were selected and their semen was collected using the abdominal massage technique. The semen was then treated with H2O2 at 0 µM, 5 µM, 50 µM and 200 µM concentrations for 0, 3, 16 and 24 hrs. The semen motility, kinematic and viability parameters were then determined using the CASA system while the viability was determined using the SYBR-14/PI staining. The Pearson’s correlation coefficient was determined to test the relationships between the levels of induced oxidative stress, period of exposure to oxidative stress inducing agent and the motility plus kinematic parameters. Our data revealed that in raw cockerel semen, there was high and positive correlations between total motility (TM), progressive motility (PM), rapid (RAP), curvilinear velocity (VCL), straight line velocity (VSL) and average path velocity (VAP) while the kinematic parameters LIN, STR, WOB, ALH and BCF had low or negative correlations with them. Furthermore, TM, PM, RAP, VCL and VSL remained highly and positively correlated with the induced oxidative stress and also, linearity (LIN), straightness (STR), wobble (WOB), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) remained negatively correlated with the induced oxidative stress, after 3 hrs. After 24 hrs, TM, PM, RAP, VCL, VSL, VAP and ALH, became negatively correlated with the induced oxidative stress while LIN, STR, WOB and BCF became positively correlated with the induced oxidative

关键词： Cockerel Oxidative Stress Kinematic Parameters Semen