检索结果-南通市图书馆

Chromosomal disorders and male infertility

Asian Journal of Andrology 2012年第1期14卷 32-39,175页

作者： gary L Harton helen g tempest Reprogenetics Livingston NJ 07039 USA Department of Human and Molecular genetics Herbert Wertheim College of Medicine Florida International University Miami FL 33132 USA

Infertility in humans is surprisingly common occurring in approximately 15% of the population wishing to start a family. Despite this, the molecular and genetic factors underlying the cause of infertility remain largely undiscovered. Nevertheless, more and more genetic factors associated with infertility are being identified. This review will focus on our current understanding of the chromosomal basis of male infertility specifically： chromosomal aneuploidy, structural and numerical karyotype abnormalities and Y chromosomal microdeletions. Chromosomal aneuploidy is the leading cause of pregnancy loss and developmental disabilities in humans. Aneuploidy is predominantly maternal in origin, but concerns have been raised regarding the safety of intracytoplasmic sperm injection as infertile men have significantly higher levels of sperm aneuploidy compared to their fertile counterparts. Males with numerical or structural karyotype abnormalities are also at an increased risk of producing aneuploid sperm. Our current understanding of how sperm aneuploidy translates to embryo aneuploidy will be reviewed, as well as the application of preimplantation genetic diagnosis （PgD） in such cases. Clinical recommendations where possible will be made, as well as discussion of the use of emerging array technology in PgD and its potential applications in male infertility.

关键词： chromosomal aneuploidy chromosomal translocation intracytoplasmic sperm injection in vitro fertilization male infertility non-disjunction preimplantation genetic diagnosis Y-chromosome microdeletion

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Significant reduction of sperm disomy in six men:effect of traditional Chinese medicine?

引用

Asian Journal of Andrology 2005年第4期7卷 419-425,页

作者： helen g. tempest Sheryl T.Homa Xiao-Ping Zhai Darren K. griffin Department of Biosciences University of KentCanterbury CT2 7NJUK Ten Harley Street London W1G 9PFUK

Aim： To test the hypothesis that levels of sperm disomy fell significantly in six men treated by traditional Chinese medicine （TCM）. Methods： Fluorescence in situ hybridization （FISH） was done on the sperm heads of six men before and during treatment by TCM. Results： There was a significant reduction in sperm disomy in all six men. This coincided with TCM treatment. Conclusion： This is the first study reporting a significant reduction in sperm disomy in men over a given time course. The fact that this coincided with TCM treatment is intriguing but no conclusions can be drawn from this until placebo-controlled clinical trials are implemented.

关键词： traditional Chinese medicine sperm disomy chromosome male infertility

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Vleiotic recombination and male infertility： from basic science to clinical reality？

引用

Asian Journal of Andrology 2011年第2期13卷 212-218页

作者： Michael Chann Patricio E Lau helen g tempest Department of Human and Molecular genetics Herbert Wertheim College of Medicine Florida International University Miami FL 33199 USA

Infertility is a common problem that affects approximately 15% of the population. Although many advances have been made in the treatment of infertility, the molecular and genetic causes of male infertility remain largely elusive. This review will present a summary of our current knowledge on the genetic origin of male infertility and the key events of male meiosis. It focuses on chromosome synapsis and meiotic recombination and the problems that arise when errors in these processes occur, specifically meiotic arrest and chromosome aneuploidy, the leading cause of pregnancy loss in humans. In addition, meiosis-specific candidate genes will be discussed, including a discussion on why we have been largely unsuccessful at identifying disease-causing mutations in infertile men. Finally clinical applications of sperm aneuploidy screening will be touched upon along with future prospective clinical tests to better characterize male infertility in a move towards personalized medicine.

关键词： fluorescent in situ hybridization immunofluorescence male infertility meiotic recombination semen parameters synap-tonemal complex

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Scoring of sperm chromosomal abnormalities by manual and automated approaches：qualitative and quantitative comparisons

引用

Asian Journal of Andrology 2010年第2期12卷 257-262页

作者： helen g. tempest Siu Yan Cheng David J. gillott Alan H. Handyside Alan R. Thomhill Darren K. griffin London Bridge Fertility Gynaecology and Genetics Centre London SEI 9RY UK Bridge genoma London SE1 9RY UK Department of Biosciences University of Kent Canterbury Kent CT2 7N J UK

It is now well known that levels of sperm disomy correlate to levels of infertility （as well as other factors）.The risk of perpetuating aneuploidy to the offspring of infertile males undergoing intracytoplasmic sperm injection （ICSI） has become a hotly debated issue in assisted reproduction;however,there remain barriers to the practical implementation of offering sperm disomy screening in a clinical setting. The major barrier is the operator time taken to analyze a statistically meaningful （sufficient） number of cells. The introduction of automated ＇spot counting＇software-hardware combinations presents a potential solution to this problem. In this preliminary validation study,we analyzed 10 patients,both manually and using a commercially available spot counter. Results show a statistically significant correlation between both approaches for scoring of sperm disomy,but no correlation is found when scoring for diploid sperm. The most likely explanation for the latter is an apparent overscoring of two closely associated sperm heads as a single diploid cell. These results,and similar further studies that will ensue,help to inform cost-benefit analyses that individual clinics need to carry out in order to decide whether to adopt sperm aneuploidy screening as a routine tool for the assessment of sperm from men requiring ICSI treatment.

关键词： aneuploidy assisted reproductive technologies automation fluorescent in situ hybridization methods scoring spermatozoa

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：