检索结果-南通市图书馆

Protease-Activatable Porphyrin Molecular Beacon for Osteoarthritis Management

Chemical & Biomedical Imaging 2023年第1期1卷 66-80页

作者： Connor Walsh Maneesha A.Rajora Lili Ding Sayaka Nakamura helal endisha Jason Rockel Juan Chen Mohit Kapoor Gang Zheng Princess Margaret Cancer Centre University Health NetworkTorontoON M5G 1L7Canada Institute of Biomedical Engineering University of TorontoTorontoON M5S 3G9Canada Schroeder Arthritis Institute University Health NetworkTorontoON M5T 0S8Canada Krembil Research Institute University Health NetworkTorontoON M5T 0S8Canada Department of Medical Biophysics University of TorontoTorontoON M5G 1L7Canada

Despite the substantial burden posed by osteoarthritis(OA)globally,difffcult challenges remain in achieving early OA diagnosis and adopting effective disease-modifying *** this study,we use a biomolecular approach to address these limitations by creating an inherently theranostic molecular beacon whose imaging and therapeutic capabilities are activated by early pathological changes in *** platform comprised(1)a peptide linker substrate for metalloproteinase-13(MMP-13),a pathological protease upregulated in OA,which was conjugated to(2)a porphyrin moiety with inherent multimodal imaging,photodynamic therapy,and drug delivery capabilities,and(3)a quencher that silences the porphyrin’s endogenous ffuorescence and photoreactivity when the beacon is *** diseased OA tissue with upregulated MMP-13 expression,this porphyrin molecular beacon(PP_(MMP13B))was expected to undergo sequence-speciffc cleavage,yielding porphyrin fragments with restored ffuorescence and photoreactivity that could,respectively,be used as a readout of MMP13 activity within the joint for early OA imaging and disease-targeted photodynamic *** study focused on the synthesis and characterization of PPMMP13B,followed by a proof-of-concept evaluation of its OA imaging and drug delivery *** solution,PP_(MMP13B)demonstrated 90%photoactivity quenching in its intact form and robust MMP-13 activation,yielding a 13-fold increase in ffuorescence *** vitro,PP_(MMP13B)was readily uptaken and activated in an MMP-13 cell expressiondependent manner in primary OA synoviocytes without exuding signiffcant *** translated into effective intra-articular cartilage(to a 50μm depth)and synovial uptake and activation of PP_(MMP13B)in a destabilization of the medial meniscus OA mouse model,yielding strong ffuorescence contrast(7-fold higher signal than background)at the diseased joint *** results provide the foundation for further exploration of porphyrin mol

关键词： Molecular Beacon Porphyrin Osteoarthritis Photodynamic Therapy Molecular Imaging Fluorescence Drug Delivery

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：