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International Journal of Minerals,Metallurgy and Materials 2023年第7期30卷 1310-1319页

作者： Jiwei Xue Huazhen Tu Jin Shi Yanni An he wan Xianzhong Bu School of Resources Engineering Xi’an University of Architecture and TechnologyXi’an 710055China State Key Laboratory of Mineral Processing Beijing 100260China Northern Engineering&Technology Corporation Dalian 116600China

The flotation separation of chalcopyrite and talc is challenging due to their similar natural floatability ***,it is usually difficult to effectively inhibit talc by adding sodium carboxymethyl cellulose(CMC)alone during chalcopyrite ***,a combined inhibitor comprising acidified sodium silicate(ASS)and CMC was employed to realize effective flotation separation of chalcopyrite and talc,and the combined inhibition mechanism was further *** results showed that adding ASS strengthened the inhibitory effect of CMC on talc and improved the separation of chalcopyrite and *** zeta potential,Fourier transform infrared,and X-ray photoelectron spectroscopy analysis indicated that CMC was mainly adsorbed on the talc surface via hydroxyl and carboxyl ***,the addition of ASS improved the adsorption of carboxyl ***,the adsorption experiments and apparent viscosity measurements revealed that adding ASS dispersed the pulp well,which reduced the apparent viscosity,improved the adsorption amount of CMC on the talc surface,and enhanced the inhibition of talc in chalcopyrite flotation.

关键词： talc chalcopyrite flotation combined inhibitor apparent viscosity

RNA interference in Colorado potato beetle(Leptinotarsa decemlineata): A potential strategy for pest control

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Journal of Integrative Agriculture 2020年第2期19卷 428-437页

作者： MA Mei-qi he wan-wan XU Shi-jing XU Le-tian ZHANG Jiang State Key Laboratory of Biocatalysis and Enzyme Engineering College of Life SciencesHubei University

Colorado potato beetle(CPB),Leptinotarsa decemlineata,is a notorious destructive pest that mainly feeds on the leaves of potato and several other solanaceous *** is widely recognized for its adaptation to a remarkable variety of host plants and diverse climates,and its high resistance to insecticides and Bacillus thuringiensis *** interference(RNAi)is a sequence-specific,endogenous gene silencing mechanism evoked by small RNA molecules that is used as a robust tool for virus and pest *** has been extensively tested for CPB management by employing various target genes and delivery *** article reviews the screening of RNAi target genes,efficient RNAi delivery systems,and factors affecting RNAi efficiency in CPB,which may help understand the mechanisms of RNAi and its application in CPB control strategy.

关键词： Colorado potato beetle double-stranded RNA pest control RNA interference RNAi delivery system

New Chiral Calixarene Derivatives: Syntheses and Their Chiral Recognition Toward Amino Acids by UV-Vis Spectroscopy

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Chemical Research in Chinese Universities 2008年第1期24卷 106-109页

作者： GU Jin-ying he wan-ping SHI Xian-fa JI Liang-nian Department of Chemistry Tongji University Shanghai 200092 F. R. China Department of Chemistry Sun Yat-sen University Guangzhou 510275 P. R. China

Three novel types of chiral calixarene derivatives 5, 8, and 10 were designed and synthesized by introducing chiral units to parent calixarenes. Their chiralities were confirmed by rotational analysis. Chiral recognition properties of these host compounds towards L- and D-threonine were studied by UV-Vis spectroscopy. The results indicated that calixarene derivatives 5 and 8 exhibited good chiral recognition capabilities toward L- or D-threonine. Although calixarene derivative 10 had no evident chiral recognition ability, the supramolecules of calixarene derivative 10 with L- or D-threonine showed a hypochromic effect or hyperchromic effect respectively. Therefore, calixarene derivative 10 might serve as a good chiral UV-indicator.

关键词： Chiral calixarene Synthesis Amino acid Chiral recognition

Synthesis,characterization,and tunable wettability of poly (ionic liquid) brushes via nitroxide-mediated radical polymerization (NMP)

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Chinese Science Bulletin 2010年第31期55卷 3562-3568页

作者： YAG Wu he XiaoJing GAO JinZhang GUO Hao he XiaoYan wan Fei ZHAO XiuLi YU Yan PEI Ben College of Chemistry and Chemical Engineering Northwest Normal University Lanzhou 730070 China

Well-defined poly(ionic liquid) brushes with tunable wettability are successfully synthesized via nitroxide-mediated radical polymerization.X-ray photoelectron spectroscopy,attenuated total reflection infrared spectroscopy and static water contact angle measurements are used to monitor each ***,nuclear magnetic resonance(1H and 13C) spectra and gel permeation chromatographic measurements reveal that there is a linear increase in polymer film thickness with polymerization time,indicating that chain growth from the surface is a controlled/"living" *** surface morphology was measured by atomic force ***,the surfaces of the poly(ionic liquid) brushes with tunable wettability and reversible switching between hydrophilicity and hydrophobicity can be easily achieved by counteranion exchange.

关键词：自由基聚合离子液体润湿性可调谐合成 NMP 表面形貌测量 X射线光电子能谱

Performance of Sn-Cu Alloy Anode at Low Temperature

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Journal of Materials Science & Technology 2005年第5期21卷 770-772页

作者： Jianguo REN, Weihua PU,Xiangming he Chunrong wan and Changyin JIANGMaterials Chemistry Lab, INET, Tsinghua University, Beijing 102201, China Materials Chemistry Lab INET Tsinghua University

Sn-Cu alloy anode was prepared by pulsed electrodeposition for lithium ion batteries, and its electrochemical performance was tested at low temperature. When temperature decreased from 25℃ to -20℃, the Sn-Cu alloy anode provided the capacity retentions of 76% in the electrolyte of 1 mol/L LiBF4/EC＋EMC＋γBL and 51% in the electrolyte of 1 mol/L LiPF6/EC＋DMC＋DEC. Under the same conditions, the graphite anode presented the capacity retentions of only 41% and 18%, respectively. The Sn-Cu alloy anode showed better performance in the electrolyte of 1 mol/L LiBF4/EC＋EMC＋γBL than in the electrolyte of 1 mol/L LiPF6/EC＋DMC＋DEC, and kept much higher capacity retention than that of graphite at -20℃. The Sn-Cu alloy anode in the electrolyte of 1mol/L LiBF4/EC＋EMC＋γBL （1：1：1, volume ratio） is promising for low temperature application of lithium batteries.

关键词： Sn-Cu alloy electrode Performance Low temperature Lithium batteries

The potential role of minimal/molecular residual disease in colorectal cancer: curative surgery, radiotherapy and beyond

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Journal of the National Cancer Center 2023年第3期3卷 203-210页

作者： Meiyi Xu Tianhao Shi Ruilian Xu Gong Chen wan he Department of Oncology Shenzhen People’s Hospital(the Second Clinical Medical CollegeJinan University/the First Affiliated HospitalSouthern University of Science and Technology)ShenzhenChina Department of Biology School of MedicineSouthern University of Science and TechnologyShenzhenChina Department of Colorectal Surgery State Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineSun Yat-Sen University Cancer CenterGuangzhouChina

Detection of minimal/molecular residual disease(MRD)based on ctDNA assay develops from hematological malignancies to solid ***,there are two mainstream assays in MRD testing technology:tumor-informed and *** colorectal cancer(CRC),MRD is used not only to monitor recurrence and predict prognosis,but also to help in clinical decision making and assessment of clinical efficacy in the settings of curative surgery,radiotherapy,chemotherapy and *** clinical trials are exploring roles of MRD in early or advanced stages of ***,we give an overview of how MRD is and will be used in CRC.

关键词： Minimal/molecular residual disease CtDNA Colorectal cancer

The Effects of Mx1 Locus on Immunity Tracts Components in Large White×Meishan F_2 Offspring

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Agricultural Sciences in China 2007年第2期6卷 244-248页

作者： LI Xiao-li he wan-ling DENG Chang-yan XIONG Yuan-zhu Colleee of Animal Science and Technology Henan University of Science ＆ Technology Luoyang 471003 P.R.China Key Laboratory of Swine Genetics and Breeding Ministry of Agricuhure/College of Animal Science and Veterinary Medicine Huazhong Agricultural University Wuhan 430070 P.R. China

The mouse Myxovirus resistance protein 1 （Mx 1） is known to be sufficient to confer resistance to influenza viruses, and genes encoding Myxovirus resistance protein 1 （Mx 1） is, therefore, an interesting candidate gene for disease resistance in farm animals. The porcine Mxl gene has already been identified and characterized based on its homology with mouse Mxl; the full-length coding region of the pig Mxl gene spans 2 545 bp （M65087） and is organized into 17 exons compared with the human ortholog mRNA. In this study, the exons 9, 10, 11 and introns 6, 9 of the porcine Mxl gene were cloned and sequenced; two SNPs were identified in exons 9, 10, 11 but none of the SNPs led to an amino acid exchange, and the other eleven variants were detected in introns 6 and 9, respectively. Differences in allele frequency among Meishan, Large White, Tibetan, Tongcheng, Huainan, and Duroc pigs were observed within intron 9, of which an A ~ G substitution at position 186 was detected as an Msp I PCR-restriction fragment length polymorphism （PCR-RFLP）. The association analysis using the Large WhitexMeishan F2 offspring suggested that the Mxl genotype was associated with variation in several immunity traits that are of interest in pig breeding. However, further investigations in more populations are needed to confirm the above concept.

关键词： pigs Mxl gene Msp I locus immunity traits

Monoclonal antibody-based serological detection of potato virus M in potato plants and tubers

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Journal of Integrative Agriculture 2020年第5期19卷 1283-1291页

作者： ZHANG Yu GAO Yan-ling he wan-qin wanG Ya-qin QIAN Ya-juan ZHOU Xue-ping WU Jian-xiang State Key Laboratory of Rice Biology Institute of BiotechnologyZhejiang UniversityHangzhou 310058P.R.China Potato Research Institute of heilongjiang Academy of Agricultural Sciences Harbin 150086P.R.China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China

Potato virus M(PVM) is one of the common and economically important potato viruses in potato-growing regions worldwide. To investigate and control this viral disease, efficient and specific detection techniques are needed. In this study, PVM virions were purified from infected potato plants and used as the immunogen to produce hybridomas secreting PVM-specific monoclonal antibodies(MAbs). Four highly specific and sensitive murine MAbs, i.e., 1 E1, 2 A5, 8 A1 and 17 G8 were prepared through a conventional hybridoma technology. Using these four MAbs, we have developed an antigen-coated plate(ACP)-ELISA, a dot-ELISA and a Tissue print-ELISA for detecting PVM infection in potato plants and tubers. PVM could be detected in infected potato plant tissue crude extracts diluted at 1:10 240(w/v, g mL^(–1)) by the dot-ELISA or at 1:163 840(w/v, g mL^(–1)) by the ACP-ELISA. The Tissue print-ELISA is the quickest and easiest assay among the three established serological assays and is more suitable for onsite large-scale sample detection. Detection results of the field-collected samples showed that PVM is currently widespread in the Yunnan and the heilongjiang provinces in China. The field sample test results of the developed serological assays were supported by the results from RT-PCR and DNA sequencing. We consider that the newly established ACP-ELISA, dot-ELISA and Tissue print-ELISA can benefit PVM detection in potato plant and tuber samples and field epidemiological studies of PVM. These assays can also facilitate the production of virus-free seed potatoes and breeding for PVM-resistant potato cultivars, leading to the successful prevention of this potato viral disease.

关键词： potato virus M monoclonal antibody serological assay antigen-coated plate(ACP)-ELISA dot-ELISA Tissue print-ELISA

Fabrication, property characterization and thermal performance of composite phase change material plates based on tetradecanol-myristic acid binary eutectic mixture/expanded perlite and expanded vermiculite for building application

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Journal of Central South University 2019年第9期26卷 2578-2595页

作者： YANG Hua CheN wan-he KONG Xiang-fei RONG Xian School of Energy and Environmental Engineering Hebei University of TechnologyTianjin 300401China School of Civil and Transportation Engineering Hebei University of TechnologyTianjin 300401China

A binary eutectic mixture composed of tetradecanol(TD)and myristic acid(MA)was maximally absorbed into the microstructures of expanded perlite(EP)and expanded vermiculite(EVMT),respectively,through a self-made vacuum adsorption roller to prepare phase change material(PCM)particle(PCP).Then EP and EVMT-based composite PCM plates were respectively fabricated through a mold pressing *** thermal property,chemical stability,microstructure and durability were characterized by differential scanning calorimeter(DSC),Fourier transform infrared spectroscope(FT-IR),scanning electron microscope(SEM)and thermal cycling tests,*** results show that both PCPs have high latent heats with 110 J/g for EP-based PCP and more than 130 J/g for EVMT-based PCP,compact microstructure without PCM leakage,stable chemical property and good *** research results have proved the feasibility for the vacuum adsorption roller used in the composite PCM *** of thermal storage performance experiment indicate that the fabricated PCM plates have better thermal inertia than common building materials,and the thermal storage performance of PCM plates has nonlinearly changed with outside air velocity and temperature ***,PCM plates show a significant potential for the practical application of building thermal storage.

关键词： thermal storage phase change material expanded perlite expanded vermiculite binary eutectic mixture

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基于角色的设计模式建模和实现方法

软件学报 2006年第4期17卷 658-669页

作者：何成万何克清武汉化工学院计算机科学与工程学院湖北武汉430073 武汉大学软件工程国家重点实验室湖北武汉430072

虽然设计模式有利于软件的重用,但当设计模式实例化时,存在模式的重叠、可追踪性以及模式代码难于重用等问题.提出一种基于角色的设计模式的建模和实现方法,在设计和实现两个层面上解决上述问题.该方法把设计模式的角色作为独立的建模元... 详细信息

虽然设计模式有利于软件的重用,但当设计模式实例化时,存在模式的重叠、可追踪性以及模式代码难于重用等问题.提出一种基于角色的设计模式的建模和实现方法,在设计和实现两个层面上解决上述问题.该方法把设计模式的角色作为独立的建模元素,在应用程序类和角色间导入RoleOf关系,使应用逻辑和模式逻辑完全分离,从而提高其重用性.使用UML的标准扩充机制,给出了RoleOf关系的元模型和语义,以及标识模式信息的构造型和标记值,以此解决模式的重叠和可追溯性等问题.通过对Java语言进行扩充(称为Rava),实现了应用逻辑和模式逻辑的动态绑定.该方法较好地解决了设计模式实例化时的模式重叠和可追踪性问题,提高了模式逻辑的可重用性,对运用设计模式开发软件有一定的指导作用.

关键词：设计模式的实例化角色 RoleOf关系 UML 元模型