检索结果-南通市图书馆

Inhibition of Ciliogenesis Enhances the Cellular Sensitivity to Temozolomide and Ionizing Radiation in Human Glioblastoma Cells

引用

Biomedical and Environmental Sciences 2022年第5期35卷 419-436页

作者： WEI Li MA Wei CAI Hui peng Shao peng TIAN Huan Bing WANG Ju Fang GAO Lan he jin peng School of Life Sciences Lanzhou UniversityLanzhou 730000GansuChina NHC Key Laboratory of Diagnosis and Therapy of Gastrointestinal Tumor Gansu Provincial HospitalLanzhou 730000GansuChina Key Laboratory of Space Radiobiology of Gansu Province&CAS Key Laboratory of heavy Ion Radiation Biology and Medicine Institute of Modern PhysicsChinese Academy of SciencesLanzhou 730000GansuChina University of Chinese Academy of Sciences Beijing 100049China

Objective To investigate the function of primary cilia in regulating the cellular response to temozolomide(TMZ)and ionizing radiation(IR)in glioblastoma(GBM).Methods GBM cells were treated with TMZ or X-ray/carbon *** primary cilia were examined by immunostaining with Arl13 b andγ-tubulin,and the cellular resistance ability was measured by cell viability assay or survival fraction *** with cilia ablation by IFT88 depletion or chloral hydrate and induction by lithium chloride,the autophagy was measured by acridine orange staining *** DNA damage repair ability was estimated by the kinetic curve ofγH2 AX foci,and the DNAdependent protein kinase(DNA-PK)activation was detected by immunostaining *** Primary cilia were frequently preserved in GBM,and the induction of ciliogenesis decreased cell *** and IR promoted ciliogenesis in dose-and time-dependent manners,and the suppression of ciliogenesis significantly enhanced the cellular sensitivity to TMZ and *** inhibition of ciliogenesis elevated the lethal effects of TMZ and IR via the impairment of autophagy and DNA damage *** interference of ciliogenesis reduced DNA-PK activation,and the knockdown of DNA-PK led to cilium formation and *** Primary cilia play a vital role in regulating the cellular sensitivity to TMZ and IR in GBM cells through mediating autophagy and DNA damage repair.

关键词： Primary cilia Glioblastoma Cellular sensitivity Temozolomide Ionizing radiation Autophagy DNA damage response DNA-PK

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Aurora A Kinase Plays a Key Role in Mitosis Skip during Senescence Induced by Ionizing Radiation

引用

Biomedical and Environmental Sciences 2023年第10期36卷 903-916页

作者： ZHANG Xu Rui ZHANG Tong Shan ZHANG Ya Nan HUA Jun Rui WANG Ju Fang he jin peng Key Laboratory of Space Radiobiology of Gansu Province&CAS Key Laboratory of heavy Ion Radiation Biology and Medicine Institute of Modern PhysicsChinese Academy of SciencesLanzhou 730000GansuChina National-Local Joint Engineering Research Center of Biodiagnostic&Biotherapy The Second Affiliated HospitalXi’an Jiaotong UniversityXi’an 710004ShaanxiChina University of Chinese Academy of Sciences Beijing 100049China

Objective To investigate the fate and underlying mechanisms of G2 phase arrest in cancer cells elicited by ionizing radiation(IR).Methods Human melanoma A375 and 92-1 cells were treated with X-rays radiation or Aurora A inhibitor MLN8237(MLN)and/or p21 depletion by small interfering RNA(si RNA).Cell cycle distribution was determined using flow cytometry and a fluorescent ubiquitin-based cell cycle indicator(FUCCI)system combined with histone H3 phosphorylation at Ser10(p S10 H3)*** was assessed using senescence-associated-β-galactosidase(SA-β-Gal),Ki67,andγH2AX *** expression levels were determined using western *** Tumor cells suffered severe DNA damage and underwent G2 arrest after IR *** damaged cells did not successfully enter M phase nor were they stably blocked at G2 phase but underwent mitotic skipping and entered G1 phase as tetraploid cells,ultimately leading to senescence in *** this process,the p53/p21 pathway is *** p21 accumulation,Aurora A kinase levels declined *** treatment confirmed that Aurora A kinase activity is essential for mitosis skipping and senescence *** Persistent p21 activation during IR-induced G2 phase blockade drives Aurora A kinase degradation,leading to senescence via mitotic skipping.

关键词： Ionizing radiation Senescence G2 arrest Tetraploid Mitosis skipping

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

GraS is critical for chloroplast development and affects yield in rice

引用

Journal of Integrative Agriculture 2020年第11期19卷 2603-2615页

作者： DU Zhi-xuan HAO Hui-ying he jin-peng WANG Jian-ping HUANG Zhou XU Jie FU Hai-hui FU Jun-ru he Hao-hua Key Laboratory of Crop Physiology Ecology and Genetic BreedingMinistry of Education/Collaboration Center for Double-season Rice Modernization ProductionJiangxi Agricultural University/Research Center of Super Rice Engineering and TechnologyJiangxi ProvinceNanchang 330045P.R.China

Leaf color has been considered an important agronomic trait in rice(Oryza sativa L.)for a long *** changes in leaf color affect the yield of *** this study,a green-revertible albino(graS)mutant was isolated from a 60Co-gamma-irradiated mutant pool of indica cultivar Guangzhan *** fine mapping indicated that graS mutant was mapped to chromosome 1,and was located in a confined region between markers ab134 and InDel 8 with genetic distances of 0.11 and 0.06 cM,*** on the annotation results,four open reading frames(ORFs)were predicted in this *** analysis revealed that LOC_Os01g55974 had a 2-bp nucleotide insertion(AA)in the coding region that led to premature termination at the 324th *** analysis and expression analysis of related genes indicated that LOC_Os01g55974 is the candidate gene of *** studied the genome and protein sequences of LOC_Os01g55974,and the data showed that GraS contains a deoxycytidine deaminase domain,which was expressed ubiquitously in all *** investigation indicated that GraS plays an essential role in the regulation of chloroplast biosynthesis,photosynthetic capacity and ***,leaf color mutant can be used as an effective marker for the purity of breeding and hybridization.

关键词： GraS bioinformatics candidate gene chlorophyll leaf color rice

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bone Loss Induced by Simulated Microgravity,Ionizing Radiation and/or Ultradian Rhythms in the Hindlimbs of Rats

引用

Biomedical and Environmental Sciences 2018年第2期31卷 126-135页

作者： ZHANG Ya Nan SHI Wen Gui LI he HUA Jun Rui FENG Xiu WEI Wen Jun WANG Ju Fang he jin peng LEI Su Wen Key Laboratory of Space Radiobiology of Gansu Province&CAS Key Laboratory of heavy Ion Radiation Biology and Medicine Institute of Modern PhysicsChinese Academy of SciencesLanzhou 730000GansuChina University of Chinese Academy of Sciences Beijing 100049China Office of health Standards Chinese Center for Disease Control and PreventionBeijing 102206China

Objective To better understand the pathological causes of bone loss in a space environment, including microgravity, ionizing radiation, and ultradian *** Sprague Dawley （SD） rats were randomly divided into a baseline group, a control group, a hindlimb suspension group, a radiation group, a ultradian rhythms group and a combined-three-factor group. After four weeks of hindlimb suspension followed by X-ray exposure and/or ultradian rhythms, biomechanical properties, bone mineral density, histological analysis, microstructure parameters, and bone turnover markers were detected to evaluate bone loss in hindlimbs of *** Simulated microgravity or combined-three factors treatment led to a significant decrease in the biomechanical properties of bones, reduction in bone mineral density, and deterioration of trabecular parameters. Ionizing radiation exposure also showed adverse impact while ultradian rhythms had no significant effect on these outcomes. Decrease in the concentration of the turnover markers bone alkaline phosphatase （bALP）, osteocalcin （OCN）, and tartrate-resistant acid phosphatase-5b （TRAP-Sb） in serum was in line with the changes in trabecular *** Simulated microgravity is the main contributor of bone loss. Radiation also results in deleterious effects but ultradian rhythms has no significant effect. Combined-three factors treatment do not exacerbate bone loss when compared to simulated microgravity treatment alone.

关键词： Bone loss Microgravity Radiation Ultradian rhythms

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

MiR-663a Inhibits Radiation-Induced Epithelium-to-Mesenchymal Transition by Targeting TGF-β1

引用

Biomedical and Environmental Sciences 2022年第5期35卷 437-447页

作者： QU Pei SHAO Zhi Ang WANG Bing he jin peng ZHANG Ya Nan WEI Wen Jun HUA Jun Rui ZHOU heng LU Dong DING Nan WANG Ju Fang Key Laboratory of Space Radiobiology of Gansu Province&Key Laboratory of heavy Ion Radiation Biology and Medicine Institute of Modern PhysicsChinese Academy of SciencesLanzhou 730000GansuChina University of Chinese Academy of Sciences Beijing 100049China

Objective miR-663 a has been reported to be downregulated by X-ray irradiation and participates in radiation-induced bystander effect via TGF-β*** goal of this study was to explore the role of mi R-663 a during radiation-induced Epithelium-to-mesenchymal transition(EMT).Methods TGF-β1 or IR was used to induce *** mi R-663 a transfection,cell migration and cell morphological changes were detected and the expression levels of mi R-663 a,TGF-β1,and EMT-related factors were *** Enhancement of cell migration and promotion of mesenchymal changes induced by either TGF-β1 or radiation were suppressed by mi R-663 ***,both X-ray and carbon ion irradiation resulted in the upregulation of TGF-β1 and downregulation of mi R-663 a,while the silencing of TGF-β1 by mi R-663 a reversed the EMT process after *** Our findings demonstrate an EMT-suppressing effect by mi R-663 a via TGF-β1 in radiationinduced EMT.

关键词： Epithelium-to-mesenchymal transition(EMT) Ionizing Radiation TGF-β1 microRNA miR-663a

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

卓越体育教师核心素养的内涵及实践探索

引用

体育学刊 2017年第2期24卷 91-95页

作者：何劲鹏东北师范大学体育学院吉林长春130024

核心素养是体育教师处理教育过程复杂性和不可预知问题的关键能力,是专业基础知识在体育教学理解和创新过程中的综合反映。将核心素养作为卓越培养行动的顶层设计加以规划,通过课程分类指导、课内外深度融合、校内外协同创新等实践探索... 详细信息

核心素养是体育教师处理教育过程复杂性和不可预知问题的关键能力,是专业基础知识在体育教学理解和创新过程中的综合反映。将核心素养作为卓越培养行动的顶层设计加以规划,通过课程分类指导、课内外深度融合、校内外协同创新等实践探索,培养具备良好的学科理解能力和教学创新能力,并起到引领作用和示范效应的体育教师,以期推进体育教师核心素养教育体系研究,提升体育教师教育质量。

关键词：学校体育卓越体育教师核心素养

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Multifunctional Nacre‑Like Nanocomposite Papers for Electromagnetic Interference Shielding via heterocyclic Aramid/MXene Template‑Assisted In‑Situ Polypyrrole Assembly

引用

Nano-Micro Letters 2025年第3期17卷 37-54页

作者： jinhua Xiong Xu Zhao Zonglin Liu he Chen Qian Yan Huanxin Lian Yunxiang Chen Qingyu peng Xiaodong he National Key Laboratory of Science and Technology On Advanced Composites in Special Environments Center for Composite Materials and StructuresHarbin Institute of TechnologyHarbin 150080People’s Republic of China

Robust, ultra-flexible, and multifunctional MXene-basedelectromagnetic interference (EMI) shielding nanocomposite filmsexhibit enormous potential for applications in artificial intelligence,wireless telecommunication, and portable/wearable electronic *** this work, a nacre-inspired multifunctional heterocyclic aramid(HA)/MXene@polypyrrole (PPy) (HMP) nanocomposite paper withlarge-scale, high strength, super toughness, and excellent tolerance tocomplex conditions is fabricated through the strategy of HA/MXenehydrogel template-assisted in-situ assembly of PPy. Benefiting from the"brick-and-mortar" layered structure and the strong hydrogen-bondinginteractions among MXene, HA, and PPy, the paper exhibits remarkable mechanical performances, including high tensile strength (309.7 MPa),outstanding toughness (57.6 MJ m−3), exceptional foldability, and structural stability against ultrasonication. By using the template effect ofHA/MXene to guide the assembly of conductive polymers, the synthesized paper obtains excellent electronic conductivity. More importantly,the highly continuous conductive path enables the nanocomposite paper to achieve a splendid EMI shielding effectiveness (EMI SE) of 54.1 dBat an ultra-thin thickness (25.4 μm) and a high specific EMI SE of 17,204.7 dB cm2g−1. In addition, the papers also have excellent applicationsin electromagnetic protection, electro-/photothermal de-icing, thermal therapy, and fire safety. These findings broaden the ideas for developinghigh-performance and multifunctional MXene-based films with enormous application potential in EMI shielding and thermal management.

关键词： MXene Remarkable mechanical properties heterocyclic aramid Electromagnetic interference shielding Polypyrrole Multifunctionality

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

应用CT三维重建评价发育性髋关节脱位儿童的髋关节形态特征

引用

中国组织工程研究 2017年第7期21卷 1092-1097页

作者：郝运何金鹏华中科技大学同济医学院附属同济医院放射科湖北省武汉市430030 华中科技大学同济医学院附属同济医院小儿外科湖北省武汉市430030

背景:发育性髋关节脱位患儿的髋关节发育情况一直沿用骨盆X射线平片进行评价,主要观察沈通氏线是否连续及骨骺核的位置,尚缺乏量化和客观的评价方法。目的:应用CT三维重建坐标系构建发育性髋关节脱位患儿的髋关节三维重建数字化模型,精... 详细信息

背景:发育性髋关节脱位患儿的髋关节发育情况一直沿用骨盆X射线平片进行评价,主要观察沈通氏线是否连续及骨骺核的位置,尚缺乏量化和客观的评价方法。目的:应用CT三维重建坐标系构建发育性髋关节脱位患儿的髋关节三维重建数字化模型,精确量化髋关节旋转中心,从而用于准确评价脱位程度。方法:收集2010年12月至2014年12月在华中科技大学附属同济医院行CT检查的单侧发育性髋关节脱位患儿,其中CT影像学资料可获取者有16例,男4例,女12例;平均年龄(4.42±2.59)岁;左侧10例,右侧6例;所有CT资料经过三维重建分析处理构建3D数字化模型,采用逆向工程软件建立三维重建坐标系,并在三维坐标系中采用球体拟合工程逆向求解方法构建髋臼的旋转中心、股骨头的旋转中心以及骨化半径,计算骨化半径比和脱位距离。结果与结论:(1)健侧髋臼旋转中心点与股骨头旋转中心点重合,其X轴、Y轴和Z轴坐标差异无显著性意义(Px>0.05,Py>0.05,Pz>0.05),患侧髋臼旋转中心与股骨头旋转中心不相重合,差异有显著性意义(Px=0.052,Py0.05);健侧股骨头与患侧股骨头的骨化半径分别(21.37±4.42)mm和(20.14±3.14)mm,差异有显著性意义(P0.05);(4)患侧髋关节的脱位距离为8.64-35.28 mm,平均(19.47±7.84)mm;(5)结果表明,应用CT三维重建可以构建发育性髋关节脱位患儿的髋关节3D数字化模型,并可以据此准确构建髋关节的旋转中心点,进而能够准确测量髋关节脱位距离。

关键词：骨疾病,发育性髋关节图像处理,计算机辅助成像,三维组织工程骨科植入物数字化骨科 CT三维重建三维坐标系数字化3D模型发育性髋关节脱位旋转中心点脱位程度

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

我国学校强制体育的合理性探析

引用

体育学刊 2015年第5期22卷 115-120页

作者：刘阳何劲鹏东北师范大学体育学院吉林长春130024 西安理工大学体育部陕西西安710048

现阶段对于学校体育改革来说,最大的麻烦还不是课程、教学的改革,而是国家教育法规、政策的强制实施遭遇了合理性的危机。基于国家建设与安全问题高度,在贯彻国家意志、回应社会诉求、体现教育尊严、反映体育规律等维度,阐述了现阶段强... 详细信息

现阶段对于学校体育改革来说,最大的麻烦还不是课程、教学的改革,而是国家教育法规、政策的强制实施遭遇了合理性的危机。基于国家建设与安全问题高度,在贯彻国家意志、回应社会诉求、体现教育尊严、反映体育规律等维度,阐述了现阶段强制的合理性,并试图通过顶层设计的立法规制、教育政策的严格问责、激发基层微改革力量等教育治理方式的构想,尽快破解我国当前学校体育改革有令不行、政策法规无法实施、说教大于法律和契约的困境,为实现我国学校体育改革的"政令通畅"构建良性的运行机制。

关键词：学校体育强制体育体质健康教育哲学中国

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

不同任务情境下定向运动员视觉记忆特征及加工策略

引用

体育学刊 2017年第1期24卷 64-70页

作者：刘阳何劲鹏东北师范大学体育学院吉林长春130024 西安理工大学体育部陕西西安710048

以专业组、中等水平组和新手组定向运动员为被试者,考察不同水平定向运动员在识别定向地图过程中记忆提取与情景转换以及地图基本符号信息记忆提取与转换的正确率、反应时,同时对记忆提取与转换的错误选项分布进行分析,评估不同水平运... 详细信息

以专业组、中等水平组和新手组定向运动员为被试者,考察不同水平定向运动员在识别定向地图过程中记忆提取与情景转换以及地图基本符号信息记忆提取与转换的正确率、反应时,同时对记忆提取与转换的错误选项分布进行分析,评估不同水平运动员视空间记忆的情景识别能力,探究运动员在地图记忆与情景识别过程中对地图信息的加工策略,为科学制定运动训练方案提供理论依据。结果显示:专业组运动员在地图信息情景的提取与转换能力上表现出了较高的水平,情景转换能力较强,识别反应能力较快;中等水平运动员在比赛地图情景中实景转换能力与专业组有一定差距,应加强比赛地图信息的实景转换能力的训练;地图基本符号的识别能力和地图信息记忆的加工策略制约着定向运动员的情景识别能力;专业组与中等组定向运动员在地图情景识别过程中更多采用整体记忆策略,新手组多采用局部记忆策略。结果说明:有针对性地改变运动员识图记忆策略将有助于改善定向运动员对地图信息加工的认识,有效提高运动员的识图能力。

关键词：运动心理学定向运动认知加工视觉记忆情景识别

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：