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American Journal of Plant Sciences 2012年第1期3卷 110-113页

作者： Hiroshi hayakawa Tunala Yukio Minamiya Katsura Ito Stephan Gale Jun Yokoyama Ryo Arakawa Tatsuya Fukuda 不详

The comparative morphology and anatomy of leaves in the serpentine endemic Aster hispidus Thunb. var. leptocladus (Makino) Okuyama and its close relative A. hispidus var. hispidus were examined. Our morphological analysis revealed that the leaves of A. hispidus var. leptocladus were significantly smaller and narrower than those of A. hispidus var. hispidus. In particular, our anatomical analysis indicated that a decreased cell number of the horizontal axis of a leaf was involved in A. hispidus var. leptocladus. This was the same process as the changing pattern of the leaves of rheophytic species, and so, similar morphological leaf modifications occurred under different selection pressures of the serpentine soil and flooding frequency of river-stream.

关键词： Anatomical Analysis Aster hispidus var. leptocladus Cell Number Minitualization Serpentine Stenophyllization

Phylogeography of Asparagus schoberioidesKunth (Asparagaceae) in Japan

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American Journal of Plant Sciences 2011年第6期2卷 781-789页

作者： Tatsuya Fukuda In-Ja Song Hokuto Nakayama Takuro Ito Akira Kanno Hiroshi hayakawa Yukio Minamiya Jun Yoko Yama 不详

To describe the phylogeographic structures of Asparagus schoberioides Kunth (Asparagaceae) in Japan, we investigated its nucleotide sequence variations with respect to its geographic distribution pattern. Sequencing of the internal transcribed spacer (ITS) 1 region in 29 samples of A. schoberioides revealed 20 polymorphic nucleotide sites. As a result, the 29 samples of A. schoberioides fell into 15 distinct haplotypes and phylogenetic analyses revealed these haplotypes fell into two major clades, Clade 1 and Clade 2. The haplotypes of Clade 1 were distributed chiefly along the Pacific Ocean side of Japan, while those of Clade 2 occurred mainly along the Japan Sea side. This result suggests that A. schoberioides has migrated via two routes in Japan.

关键词： Asparagus schoberioides Internal Transcribed Spacer (ITS) Phylogeny Phylogeography

Introgressive Hybrids of Arisaema sikokianumand A. tosaense(Araceae) Confirmed through Nuclear and Chloroplast DNA Comparisons

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American Journal of Plant Sciences 2011年第3期2卷 303-307页

作者： Hiroshi hayakawa Hidenori Hamachi Kanako Matsuyama Yuko Muramatsu Yukio Minamiya Katsura Ito Jun Yokoyama Tatsuya Fukuda 不详

Morphologically putative introgressive hybrids of Arisaema sikokianum Franch. et Sav. and A. tosaense Makino were newly found in Kochi and Tokushima Prefectures in Japan. All the individuals have the same morphological characteristics as A. tosaense excluding a purple spathe. Molecular analysis using PCR-RFLP of internal transcribed spacer (ITS) in nuclear DNA (nrDNA) indicates that these putative introgressive hybrids have the same pattern as A. tosaense. Moreover, the sequences of chloroplast DNA (cpDNA) of the putative introgressive hybrids were identical to A. sikokianum from Kochi Prefecture and A. tosaense from Tokushima Prefecture. The results suggest that the plants are introgressive hybrids of A. sikokianum and A. tosaense and that they have highly exchanged genes with A. tosaense.

关键词： Araceae Arisaema A. sikokianum A. tosaense Chloroplast Capture Introgression ITS Molecular Analysis PCR-RFLP Trn

Use of hybrid chitosan membranes and human mesenchymal stem cells from the Wharton jelly of umbilical cord for promoting nerve regeneration in an axonotmesis rat model

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Neural Regeneration Research 2012年第29期7卷 2247-2258页

作者： Andrea Gärtner Tiago Pereira Maria Joāo Simōes Paulo AS Armada-da-Silva Miguel L França Rosa Sousa Simone Bompasso Stefania Raimondo Yuki Shirosaki Yuri Nakamura Satoshi hayakawa Akiyoshi Osakah Beatriz Porto Ana Lúcia Luís Artur SP Varejāo Ana Colette Maurício Animal Science and Study Centre/Food and Agrarian Sciences and Technologies Institute Porto University4099-003 PortoPortugal Institute of Biomedical Sciences Abel Salazar Veterinary Clinics DepartmentPorto UniversityPorto 4099-003Portugal Faculty of Human Kinetics Technical University of LisbonCruz Quebrada-Dafundo1499-002Portugal Institute of Biomedical Sciences Abel Salazar Cytogenetic DepartmentPorto UniversityPorto 4099-003Portugal Neuroscience Institute of the Cavalieri Ottolenghi Foundation Orbassano 10043TurinItaly Department of Clinical and Biological Sciences University of TurinOrbassano 10010TurinItaly Graduate School of Natural Science and Technology Okayama UniversityOkayama 700-8530Japan Faculty of Engineering Okayama UniversityOkayama 700-8530Japan Department of Veterinary Sciences Research Centre in SportsHealth and Human DevelopmentUniversity of Trás-os-Montes and Alto DouroVila Real 5001-801Portugal

Many studies have been dedicated to the development of scaffolds for improving post-traumatic nerve regeneration. The goal of this study was to assess the effect on nerve regeneration, associating a hybrid chitosan membrane with non-differentiated human mesenchymal stem cells isolated from Wharton＇s jelly of umbilical cord, in peripheral nerve reconstruction after crush injury. Chromosome analysis on human mesenchymal stem cell line from Wharton＇s jelly was carried out and no structural alterations were found in metaphase. Chitosan membranes were previously tested in vitro, to assess their ability in supporting human mesenchymal stem cell survival, expansion, and differentiation. For the in vivo testing, Sasco Sprague adult rats were divided in 4 groups of 6 or 7 animals each： Group 1, sciatic axonotmesis injury without any other intervention （Group 1-Crush）; Group 2, the axonotmesis lesion of 3 mm was infiltrated with a suspension of 1 250 -1 500 human mesenchymal stem cells （total volume of 50 pL）（Group 2-CrushCell）; Group 3, axonotmesis lesion of 3 mm was enwrapped with a chitosan type Ill membrane covered with a monolayer of non-differentiated human mesenchymal stem cells （Group 3-CrushChitlllCell） and Group 4, axonotmesis lesion of 3 mm was enwrapped with a chitosan type III membrane （Group 4-CrushChiUll）. Motor and sensory functional recovery was evaluated throughout a healing period of 12 weeks using sciatic functional index, static sciatic index, extensor postural thrust, and withdrawal reflex latency. Stereological analysis was carded out on regenerated nerve fibers. Results showed that infiltration of human mesenchymal stem cells, or the combination of chitosan membrane enwrapment and human mesenchymal stem cell enrichment after nerve crush injury provide a slight advantage to post-traumatic nerve regeneration. Results obtained with chitosan type III membrane alone confirmed that they significantly improve post-traumatic axonal regrowth and may represent

关键词： stem cells mesenchymal stem cells Wharton jelly umbilical cord biomaterials chitosan axonotmesis functional analysis rat karyotype analysis stereological analysis

Evaluation of genetic diversity and conservation priorities for Egyptian chickens

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Open Journal of Animal Sciences 2012年第3期2卷 183-190页

作者： Sherif Ramadan Boniface B. Kayang Eiji Inoue Keijiro Nirasawa Hiroshi hayakawa Shin’ichi Ito Miho Inoue-Murayama Animal Breeding and Reproduction Research Team National Institute of Livestock and Grassland Science Tsukuba Japan Department of Animal Science College of Agriculture and Consumer Sciences University of Ghana Legon Ghana Faculty of Applied Biological Sciences Gifu University Gifu Japan Graduate School of Science Kyoto University Kyoto Japan Poultry Research Department Gifu Prefectural Livestock Research Institute Seki Japan Wildlife Research Center of Kyoto University Kyoto Japan

In this study, 21 microsatellite markers were used to genotype 196 Egyptian local chickens obtained from Fayoumi (n = 35), Dandarawy (n = 30), Baladi (n = 29), Sinai (n = 30), El-Salam (n = 36), and Golden Montazah (n = 36) strains. The results were compared to two pure commercial chicken populations reared in Japan-White Leghorn (n = 42) and Rhode Island Red (n = 43). A total of 162 alleles were observed, with an average of 7.7 alleles per locus. The average expected heterozygosity for the Egyptian chickens was 0.595. The closest pairwise Nei’s genetic distance was recorded between Sinai and Golden Montazah (0.038) and the smallest pairwise FST value (0.006) was observed between Baladi and Sinai. The most probable structure clustering of the eight studied populations was at K = 6. Baladi, Sinai and Golden Montazah strains were clustered together forming admixed mosaic cluster. Dandarawy ranked firstly and contributed the most to aggregate genetic diversity based on two prioritization methods. The information resulting from this study may be used as an initial guide to design further investigations for development of sustainable genetic improvement and conservation programs for the Egyptian chicken genetic resources.

关键词： Conservation Egyptian Chickens Genetic Diversity Microsatellite Population Structure

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应用激光闪光法测定半导体融体的热扩散率

工程热物理学报 2005年第1期26卷 140-142页

作者：唐大伟荒木信幸早川泰宏中国科学院工程热物理研究所北京100080 日本静冈大学工学部日本静冈大学电子工学研究所

本文开发了半导体融体的热扩散率的测量装置。通过引入石英玻璃试样容器将激光闪光法扩展到适合于融体试样。首先在实验及理论上研究了石英玻璃层对试样的热辐射及温度相应的影响,结果发现在室温以上温度范围,石英玻璃层的存在只减小红... 详细信息

本文开发了半导体融体的热扩散率的测量装置。通过引入石英玻璃试样容器将激光闪光法扩展到适合于融体试样。首先在实验及理论上研究了石英玻璃层对试样的热辐射及温度相应的影响,结果发现在室温以上温度范围,石英玻璃层的存在只减小红外辐射探测器的信号幅度但不改变时阃变化历程。由闪光法的原理,包含石英玻璃层的复合试样则可以作为单层试样处理。根据这一原理,我们首次对三元红外半导体融体In1-xGaxSb在不同组分时,温度范围800K至1200 K的热扩散率进行了测量。

关键词：半导体融体激光闪光法石英玻璃容器热扩散率 In1-xGazSb

Crystal Growth of InGaAs by Thermal Gradient,Vertical and Rotational Bridgman Methods

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人工晶体学报 1991年第Z1期 242-242页

作者： Teltsou OZAWA,Masahiko ANDO,Yasuhiro hayakawa,Masashi KUWAGAWA Research Institute of Electronics Shizuoka University Johoku 3-5-1 Hamamatsn Shizuoka 432 Japan Research Institute of Electronics Shizuoka University Johoku 3-5-1 Hamamatsn Shizuoka 432 Japan Research Institute of Electronics Shizuoka University Johoku 3-5-1 Hamamatsn Shizuoka 432 Japan Research Institute of Electronics Shizuoka University Johoku 3-5-1 Hamamatsn Shizuoka 432 Japan

ZnSe is a sereiconductor with a wide direct energy gap（2.7 eV）at room temperature and is suitable for blue light emitting diodes, lasers and other opto electronic devices. GaAs has been mainly used as a substrate of ZnSe. However. there exists a 0.27％lattice ismatch between them. This mismateh causes the generation of many dislocations and defects, and as a result the quality of ZnSe layers becomes poor. InxGa1-xAs can be adjusted its lattice con

关键词： In As Crystal Growth of InGaAs by Thermal Gradient,Vertical and Rotational Bridgman Methods

Variation in the Leaf Number of Arisaema iyoanumsubsp. nakaianumand A. ovalevar. ovale(Araceae)

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American Journal of Plant Sciences 2013年第1期4卷 38-41页

作者： Kanako Matsuyama Hiroshi hayakawa Yuko Muramatsu Katsura Ito Shin-Ichi Tebayashi Ryo Arakawa Jun Yokoyama Tatsuya Fukuda Faculty of Agriculture Kochi University Nankoku Japan Faculty of Science Yamagata University Yamagata Japan Graduate School of Integrated Arts and Sciences Kochi University Nankoku Japan

Arisaema iyoanum Makino subsp. nakaianum (Ohba) H. Ohashi et J. Murata and A. ovale Nakai var. ovale are known to have one-leaved phenotype in both males and females;however, we discovered two-leaved individuals of these species. To elucidate the relationship between growth stage and leaf number of A. iyoanum subsp. nakaianum and A. ovale var. ovale, we conducted a morphological analysis of these plants. Our analysis suggested that the two-leaved individuals of A. iyoanum subsp. nakaianum and A. ovale var. ovale appeared only at the female phase. This suggested that one-leaved A. iyoanum subsp. nakaianum and A. ovale var. ovale individuals could not store the resources and hence changed to two-leaved individuals. This transformation could be explained by the fact that these species occur at high altitudes in mountain areas or regions covered in snow of the Japan Sea side, and their flowering time is also late compared with that in other sympatric Arisaema species.

关键词： Arisaema iyoanum subsp.nakaianum A. ovale var. ovale Leaf Number

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大众心理学丛书 102 语言与人生

Morphological Study of Glochidion obovatumunder Heavy Browsing Pressure by Sika Deer

1985年

作者：早川博士（S.I.hayakawa）邓海珠译

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American Journal of Plant Sciences 2014年第9期5卷 1345-1351页

作者： Shogo Takei Kazuhiro Yoshioka Satoru Yamada Hiroshi hayakawa Jun Yokoyama Katsura Ito Shinichi Tebayashi Ryo Arakawa Tatsuya Fukuda Faculty of Agriculture Kochi University Kochi Japan Faculty of Science Yamagata University Yamagata Japan

To clarify the modification of physical defences of Glochidion obovatum Sieb. et Zucc., we compared the length of spine-like branches and frequency of lateral branches of G. obovatum between Kashima Island, which supports a high density of sika deer (Cervus nippon), and its neighbouring areas. The length of spine-like branches of G. obovatum in Kashima Island was not significantly longer than that of the neighbouring areas, but the frequency of lateral branches on the island was higher than that of the neighbouring areas. Since the branches of G. obovatum are heavily foraged by the sika deer, the shrub form of the species has evolved into the bonsai form, suggesting increased frequency of occurrence of lateral branches, which could develop as shoot-like branches. Thus, the observed features are facultative defence mechanisms of G. obovatum against heavy browsing in a small-sized island with very high density of sika deer.

关键词： Deer Defence Frequency Herbivore Island Sika