检索结果-南通市图书馆

Comparison of Fecal Methanogenic Archaeal Community Between Erhualian and Landrace Pigs Using Denaturing Gradient Gel Electrophoresis and Real-Time PCR Analysis

引用

Journal of Integrative Agriculture 2014年第6期13卷 1340-1348页

作者： SU Yong hauke smidt ZHU Wei-Yun Laboratory of Gastrointestinal Microbiology College of Animal Science and TechnologyNanjing Agricultural University Laboratory of Microbiology Agrotechnology and Food Sciences GroupWageningen University

Erhualian and Landrace breeds are typical genetically obese and lean pigs, respectively. To compare the fecal methanogenic Archaeal community between these two pig breeds, fecal samples from different growth phase pigs were collected and used for PCR-denaturing gradient gel electrophoresis （DGGE） with two primer pairs （344fGC/519r and 519f/915rGC） and real-time PCR analysis. Results showed that a better separation and higher quality of bands pattern were obtained in DGGE proifles using primers 344fGC/519r as compared with primers 519f/915rGC. Sequencing of DGGE bands showed that the predominant methanogens in the feces of Erhualian and Landrace pigs belonged to Methanobrevibacter spp. and Methanosphaera spp. Real-time PCR analysis revealed that there was no signiifcant difference in the numbers of fecal total methanogens between Erhualian and Landrace pigs;however, pig growth phase affected the numbers of 16S rRNA genes of total methanogens and Methanobrevibacter smithii. Dissociation curves of methyl coenzyme-M reductase subunit A （mcrA） gene fragments ampliifed with real-time PCR showed all samples possessed a single peak at 82＆#176;C, which might be associated with M. smithii. Samples from the same growth phase of each breed showed good replicative dissociation curves. The results suggest that the growth phase （including diet factor） other than genotype of pig may affect the fecal methanogenic Archaeal community of pigs.

关键词： methanogenic Archaea Erhualian pig Landrace pig Methanobrevibacter smithii

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The dark side of technological advances in analysis of microbial ecosystems

引用

Journal of Animal Science and Biotechnology 2019年第3期10卷 579-589页

作者： Mick Bailey Amy Thomas Ore Francis Christopher Stokes hauke smidt Bristol Veterinary School University of BristolLangford HouseLangfordBristol BS40 5DUUK Laboratory of Microbiology Wageningen University&ResearchNL-6708 WageningenWENetherlands

Recent technological advances mean that samples from animal experiments may be analysed more cheaply,more easily and with a much greater return of data than *** groups are frequently faced with a choice of continuing to use established technology in which they may have made a significant investment of time and resources,and have significant amounts of reference data,or switching to new technology where reference data may be *** from cost,the choice needs to be based on a comparison between the increase in data available from future experiments by switching and the value of comparison with reference data from historical experiments analysed with earlier *** approach to this problem is to ensure that sufficient quantity and variety of samples are taken from each experiment and appropriately stored to allow re-establishment of a sufficiently large reference set and to avoid the need to repeat animal *** establishment of ‘biobanks' of experimental material will require funding for infrastructure,consistent storage of metadata and,importantly,horizon-scanning to ensure that samples are taken appropriately for techniques which will become accessible in *** biobanks are a recognised resource in human medicine,where the value of samples increases as more analysis is carried out and added to the metadata.

关键词： Biobanking Experimental design Horizon-scanning Microbiome Replacement Reduction Refinement Technological advances

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cultivation-Independent Analysis of the Development of the Lactobacillus *** in the Intestinal Tract of Newborn Piglets

引用

Agricultural Sciences in China 2011年第3期10卷 438-447页

作者： YAO Wen ZHU Wei-yun hauke smidt Martin W A Verstegen College of Animal Science and Technology Nanjing Agricultural UniversityNanjing210095PR．China Laboratory of Microbiology Wageningen University Wageningen 6703 CT The Netherlands Department of Animal Sciences Wageningen University Wageningen 67004H The Netherlands

Molecular diversity and development of the Lactobacillus community in the intestinal tract, as influenced by age and intestinal compartment, were studied in one litter of 12 conventionally raised piglets. Piglets were euthanized at each week （3 animals per time）. Digesta and tissue samples from stomach, duodenum, jejunum, ileum, caecum, colon, and rectum were collected and analysed by using 16S ribosomal RNA-based methods. DGGE （denaturing gradient gel electrophoresis） profiles revealed that the Lactobacillus communities throughout the GI tract from duodenum to rectum showed good stability at same age. This indicates that fecal Lactobacillus communities can effectively represent the intestinal community. Two dominant bands were found in tissue samples of the small intestine, suggesting that the lactobacilli can adhere to the small intestinal wall. The Lactobacillus communities in different GI tract compartments developed over time. A successional change of Lactobacillus communities was observed from birth, through creep feeding to one week after weaning, showing a trend from simple to complex and back to simple. Furthermore, a clone library of Lactobacillus spp. 16S rRNA gene sequences were generated from jejunal and colonic chymes. Six dominant DGGE bands generated from jejunal chymes were matched with sequences that show 94-98% similarity to the bands derived from L. reuteri, L. delbrueckii, and L. crispatus. Seven dominant DGGE bands generated from colon chymes were matched with sequences that show 88-99% similarity to those derived from L. reuteri, L. delbrueckii, L. amylovorus/L. sobrius, and L. acidophilus. Amplicons related to L. reuteri were found in all DGGE fingerprints from jejunal digesta of age of weeks 1, 3, and 4. Amplicons related to L. amylovorus/L. sobrius were present in all DGGE fingerprints from colonic digesta of age of week 1, 3, and 4. Amplicons related to L. delbrueckii were found before weaning, L. crispatus after creep feeding before weani

关键词： molecular diversity 16S rRNA gene denaturing gradient gel electrophoresis (DGGE) Lactobacillus community piglets

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cereal type and combined xylanase/glucanase supplementation influence the cecal microbiota composition in broilers

引用

Journal of Animal Science and Biotechnology 2022年第5期13卷 1531-1542页

作者： Dimitrios Kouzounis Jannigje G.Kers Natalia Soares hauke smidt Mirjam A.Kabel Henk A.Schols Laboratory of Food Chemistry Wageningen University&Research6708 WG WageningenThe Netherlands Laboratory of Microbiology Wageningen University&Research6708 WE WageningenThe Netherlands Huvepharma NV 2600 BerchemBelgium

Dietary fiber-degrading enzyme supplementation in broilers aims at off-setting the anti-nutritive effect of non-starch polysaccharides and at promoting broiler ***,we demonstrated that xylanase/glucanase addition in wheat-based diet improved nutrient digestibility,arabinoxylan fermentability and broiler ***,maize arabinoxylan was found to be recalcitrant to xylanase *** findings suggested that enzyme-mediated improvement of nutrient digestion and carbohydrate fermentation depended on the cereal type present in the diet,and may have contributed to broiler ***,we aimed at further investigating the link between dietary enzymes and carbohydrate fermentation in broilers,by studying the impact of enzyme supplementation in cereal-based diets,to the microbial communities in the ileum and ceca of *** that purpose,96 one-day-old male broilers were randomly reared in two pens and received either wheat-based or maize-based starter and grower *** d 20,the broilers were randomly assigned to one out of four dietary *** broilers received for 8 d the wheat-based or maize-based finisher diet as such(Control treatments;WC,MC)or supplemented with a xylanase/glucanase combination(Enzyme treatments;WE,ME).At d 28,samples from the digestive tract were collected,and the ileal and cecal microbiota composition was determined by 16S ribosomal RNA gene amplicon sequencing.A similar phylogenetic(alpha)diversity was observed among the four treatments,both in the ileal and the cecal ***,a similar microbial composition in the ileum(beta diversity)was observed,with lactobacilli being the predominant community for all *** contrast,both cereal type and enzyme supplementation were found to influence cecal *** type of cereal(i.e.,wheat or maize)explained 47% of the total variation in microbial composition in the *** stratifying the analysis per cereal type revealed differences in microbio

关键词： Arabinoxylo-oligosaccharides Broiler gut microbiota Cereal non-starch polysaccharides Feed enzymes Fiber fermentation Prebiotics 16S rRNA

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Gut microbiota research nexus:One Health relationshipbetween human,animal,and environmental resistomes

引用

mLife 2023年第4期2卷 350-364页

作者： Yuhao Fu Qingyuan Dou Kornelia Smalla Yu Wang Timothy A.Johnson Kristian K.Brandt Zhi Mei Maoyuan Liao Syed A.Hashsham Andreas Schäffer hauke smidt Tong Zhang Hui Li Robert Stedtfeld Hongjie Sheng Benli Chai Marko Virta Xin Jiang Fang Wang Yong-Guan Zhu James M.Tiedje State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjingChina University of Chinese Academy of Sciences BeijingChina Julius Kühn Institute(JKI)Federal Research Centre for Cultivated Plants BraunschweigGermany Department of Animal Sciences Purdue UniversityWestLafayetteIndianaUSA Section for Microbial Ecology and Biotechnology Department of Plant and Environmental SciencesUniversity of CopenhagenFrederiksberg CDenmark Sino-Danish Center(SDC) BeijingChina Department of Microbiology University of HelsinkiHelsinkiFinland Department of Plant Soil and MicrobialSciencesCenter for Microbial EcologyMichigan State UniversityMichiganUSA Department of Civil and Environmental Engineering Michigan State UniversityMichiganUSA Institute for Environmental Research RWTH Aachen UniversityAachenGermany Laboratory of Microbiology Wageningen University&ResearchWageningenThe Netherlands Environmental Microbiome Engineering and Biotechnology Laboratory Center for Environmental Engineering ResearchDepartment of Civil EngineeringTheUniversity of Hong KongPokfulamHong KongChina Institute of Agricultural Resources and Environment Jiangsu Academy of Agricultural SciencesNanjingChina KeyLaboratory of Urban Environment and Health Institute of Urban EnvironmentChinese Academy of SciencesXiamenChina State Key Laboratory of Urban and Regional Ecology Chinese Academy of SciencesBeijingChina

The emergence and rapid spread of antimicrobial resistance is of global public health *** gut microbiota harboring diverse commensal and opportunistic bacteria that can acquire resistance via horizontal and vertical gene transfers is considered an important reservoir and sink of antibiotic resistance genes(ARGs).In this review,we describe the reservoirs of gut ARGs and their dynamics in both animals and humans,use the One Health perspective to track the transmission of ARG-containing bacteria between humans,animals,and the environment,and assess the impact of antimicrobial resistance on human health and socioeconomic *** gut resistome can evolve in an environment subject to various selective pressures,including antibiotic administration and environmental and lifestyle factors(e.g.,diet,age,gender,and living conditions),and interventions through *** to reduce the abundance of clinically relevant antibiotic-resistant bacteria and their resistance determinants in various environmental niches are needed to ensure the mitigation of acquired antibiotic *** the help of effective measures taken at the national,local,personal,and intestinal management,it will also result in preventing or minimizing the spread of infectious *** review aims to improve our understanding of the correlations between intestinal microbiota and antimicrobial resistance and provide a basis for the development of management strategies to mitigate the antimicrobial resistance crisis.

关键词： antibiotic antimicrobial resistance gut microbiota modulatory strategies One Health

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

16S rRNA基因技术比较二花脸猪和长白猪粪样菌群区系

16S rRNA基因技术比较二花脸猪和长白猪粪样菌群区系

引用

低碳经济与高效养殖——第六次全国饲料营养学术研讨会暨动物营养学分会成立三十周年大会

作者：苏勇罗玉衡张伶俐 Odette N.PEREZ-GUTIERREZ hauke smidt 朱伟云南京农业大学消化道微生物研究室瓦赫宁根大学微生物实验室

二花脸猪和长白猪分别是典型的脂肪型和瘦肉型猪,有研究表明,脂肪型猪(如梅山猪)粪样中的拟杆菌门(Bacteroidetes)在总细菌中的比例显著低于瘦肉型猪(长白猪),并且体脂含量与拟杆菌门在总细菌含量呈现相关性。然而,对于脂肪型和瘦肉型... 详细信息

二花脸猪和长白猪分别是典型的脂肪型和瘦肉型猪,有研究表明,脂肪型猪(如梅山猪)粪样中的拟杆菌门(Bacteroidetes)在总细菌中的比例显著低于瘦肉型猪(长白猪),并且体脂含量与拟杆菌门在总细菌含量呈现相关性。然而,对于脂肪型和瘦肉型猪肠道中其他菌群的系统比较研究还未见报道。本研究二花脸猪和长白猪分别是典型的脂肪型和瘦肉型猪,有研究表明,脂肪型猪(如梅山猪)粪样中的拟杆菌门(Bacteroidetes)在总细菌中的比例显著低于瘦肉型猪(长白猪),并且体脂含量与拟杆菌门在总细菌含量呈现相关性。然而,对于脂肪型和瘦肉型猪肠道中其他菌群的系统比较研究还未见报道。本研究

关键词： 16SrRNA 二花脸猪长白猪菌群区系

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

小鼠结肠细菌群落对不同类型日粮纤维的特异性响应

小鼠结肠细菌群落对不同类型日粮纤维的特异性响应

引用

中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会

作者：张玲李华 hauke smidt Andre-Denis G.Wright 陈代文张克英余冰何军毛湘冰郑萍虞洁罗钧秋黄志清罗玉衡四川农业大学动物营养研究所教育部动物抗病营养学重点实验室荷兰瓦赫宁根大学微生物学实验室亚利桑那大学动物科学与比较医学院

本研究旨在探讨小鼠结肠细菌群落结构分别对可溶性日粮纤维(soluble dietary fibers,SDF)和不可溶性日粮纤维(insoluble dietary fibers,IDF)的特异性响应,并初步探索小鼠结肠是否存在利用两种日粮纤维的核心菌群。将28只8周龄健康雄性B... 详细信息

本研究旨在探讨小鼠结肠细菌群落结构分别对可溶性日粮纤维(soluble dietary fibers,SDF)和不可溶性日粮纤维(insoluble dietary fibers,IDF)的特异性响应,并初步探索小鼠结肠是否存在利用两种日粮纤维的核心菌群。将28只8周龄健康雄性BALB/c小鼠按体重相近原则分为4个处理:对照组(不额外添加日粮纤维)、G组(SDF组,添加28%燕麦β葡聚糖)、M组(IDF组,添加20%微晶纤维素)和GM组(14%燕麦β葡聚糖+10%微晶纤维素)。21天试验期结束后处死,采集结肠食糜,采用Illumina Miseq测序平台检测小鼠结肠内容物细菌群落结构,实时荧光定量(qPCR)技术对主要差异菌群进行定量。结果:1)多样性分析表明,G组小鼠细菌的Chao 1指数和Shannon指数低于其他组(PM组和GM组>对照组(PM组和GM组>G组(P0.05)。结论:小鼠结肠存在特异性利用SDF和IDF的核心菌群,且结肠菌群结构可根据不同类型日粮纤维做出适应性改变;而SDF摄入量过高可能促使潜在致病菌增长。进一步分析表明,食用两种类型纤维更利于宿主肠道健康。

关键词：日粮纤维细菌群落结肠 BALB/c小鼠

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：