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Characterization of Agro-diversity by Seed Storage Protein Electrophoresis:Focus on Rice Germplasm from Uttarakhand Himalaya,India

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Rice science 2010年第2期17卷 122-128页

作者： Arun JUGRAN Indra D.bhatt Ranbeer S.RAWAL G.B.Pant Institute of Himalayan Environment and Development Kosi-Katarmal

The characteristics of 48 rice varieties from Uttarakhand Himalaya, India were detected by morphological and biochemical markers. The grains of the selected rice varieties varied in their morphological （grain length, grain width and grain weight） and biochemical characters （sodium dodecyl sulfate polyacrylamide gel electrophoresis, SDS-PAGE）. Based on the presence of 70, 65, 60, 57, 37-39, 22-23, 13 and 10 kDa protein bands in the 48 rice varieties, seven types of profiles were identified. An unweighted pair group average method with arithmetic mean （UPGMA） dendrogram based on cluster analysis of genetic similarity of the protein bands showed two distinct groups with 1%-78% similarity coefficients. The presence of characteristic bands in selected varieties is a useful parameter for identification of rice germplasm.

关键词： rice seed storage protein sodium dodecyl sulfate polyacrylamide gel electrophoresis genetic diversity polymorphism varietal identification

Impact of hospital volume on outcomes of percutaneous ASD/PFO closure in pediatric patients

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World Journal of Pediatrics 2018年第4期14卷 364-372页

作者： Parth bhatt Achint Patel Varun Kumar Anusha Lekshminarayanan Viranchi Patel Srilatha Alapati Zeenia Cyrus Billimoria Texas Tech University Health Sciences Center AmarilloTXUSA University of Arkansas for Medical Sciences Little RockARUSA Mt.Sinai St.Luke's Roosevelt Hospital Center New YorkNYUSA Mercy Health CincinnatiOHUSA Tulane University School of Public Health and Tropical Medicine New OrleansLAUSA University of Washington Seattle Children's Hospital4800 Sand Point Way NESeattleWA 98105USA

Background We investigated the effect of hospital volume on percutaneous closure of atrial septal defect/patent foramen ovale (ASD) among pediatric patients. Methods We identified patients undergoing percutaneous closure of ASD with device using International Classification of Diseases, 9th Revision, Clinical Modification (ICD-9-CM) procedure code 35.52 from the National Inpatient Sample, years 2002–2011. Patients with age ≤ 18 years and primary diagnosis code 745.5 for ASD were included. Hospital volume was calculated using unique identification numbers and divided into tertiles for analysis. Multivariate regression analysis was performed to determine independent predictors of procedure-related complications which were coded using specific codes released by Healthcare Cost and Utilization Project. Results 6162 percutaneous ASD closure procedures were analyzed. There was no mortality associated with percutaneous ASD closure. Cardiac complications (9.5%) were most common. On multivariate analysis, age increment of 3 years decreased the odds of developing complications (OR 0.83, 95% CI 0.79–0.87,P < 0.001). Odds of developing complications in the 2nd (OR 0.74, 95% CI 0.62–0.89,P = 0.007) and 3rd tertiles (OR 0.34, 95% CI 0.27–0.42,P < 0.001) were lower as compared to the 1st tertile of hospital volume. Conclusion Increasing annual hospital volume is an independent predictor of lower complication rates in percutaneous ASD closure cases with no associated mortality in pediatric patients.

关键词： Atrial septal defect Cardiac catheterization Children

Genotype × environmental interactions for analyzing adaptability and stability in different clones of Dalbergia sissoo Roxb.

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Journal of Forestry Research 2012年第1期23卷 65-74页

作者： A.Kumar A.bhatt S.Ravichandran V.Kumar S.Dobhal Division of Genetics and Tree Propagation Forest Research InstituteDehradun 248195India Directorate of Rice Research HyderabadAndhra Pradesh 528133India State Forest Department HoshiarpurPunjab 146001India

In total 36 superior clones of Dalbergia sissoo Roxb., screened from 300 selections conducted in natural and growing range of India and Nepal, were multiplied using single nodal cuttings and estab- lished to evaluate genotypexenvironmental interactions for adaptability and stability at the age of 30 months in three geographical locations in the state of Punjab, India. Clone 124 had maximum adaptability and stability （bi = 1.04） to perform exceedingly well over the locations. Clones 36 and 1 were stable with mean regression coefficient of 0.84 and 1.22, respectively. Nonetheless, clone 4 1 performed exceedingly well for all the characters to attain maximum population mean, and the perform- ance varied substantially across the locations. Therefore, clone 41 was considered as productive but non-adaptive clone. Though some of the clones were sensitive to sites, 14 clones for height, 16 for collar diameter, 12 for DBH and 7 for volume were relatively un-sensitive with higher regression coefficient. Nonetheless, clone 124 was the most Stable with average bi value of 1.04 and productive, which could play an important role in future breeding and commercial deployment of stable and produc- tive planting stock of Dalbergia sissoo.

关键词： Dalbergia sissoo Roxb. GxE interactions stability parame-ters adaptability superior genotypes

Sustainability issues on rice–wheat cropping system

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International Soil and Water Conservation Research 2016年第1期4卷 64-74页

作者： Rajan bhatt Surinder S Kukal Mutiu A Busari Sanjay Arora Mathura Yadav Farm Advisory Service Scheme Tarn TaranPunjab Agricultural UniversityLudhiana-141004PunjabIndia b Department of Soil Science Punjab Agricultural University141004 LudhianaPunjabIndia Central Soil Salinity Research Institute(ICAR) RRSLucknowUttar PradeshIndia Department of Soil Science and Land Management Federal University of AgricultureP.M.B.2240 AbeokutaNigeria National Wheat Research Program BhairahawaRupandehiNepal

Rice–wheat cropping system(RWCS)of the South Asia is labour-,water-,capital-and energy-intensive,and become less profitable as the availability of these resources *** could be further aggravated with deterioration of soil structure,declining underground water and lesser land and water productivity which ultimately are threat in front of sustainable and profitable RWCS in the *** improving the profits,production and sustainability of this sequence-a paradigm shift is *** recommended different resource-conserving technologies(RCTs)*** tillage,laser levelling,irrigation based on soil matric potential,bed planting,direct seeding,mechanical transplanting of rice and crop diversification for this *** technologies are site specific and before selecting any particular RCT for a particular region,soil texture and agro-climatic conditions must be considered.A solitary approach/RCT might not be effective to solve the upcoming issue of producing more food grains with inadequate available water and ***,an integrated approach is *** before implementing any approach,different issues relating to RWCS must be discovered,considered and addressed in a holistic *** this review,an attempt was made to highlight different issues resulted from the practise of intensive rice–wheat cropping sequence of the region,which must be considered while framing and implementing any integrated approach/project such as conservation agriculture for improving the productions,profits and sustainability of RWCS in the region.

关键词： Rice Wheat Sustainability issues Climate smart agriculture

Controls on Gosaikunda Lake Chemistry within Langtang National Park in High Himalaya, Nepal

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International Journal of Geosciences 2014年第10期5卷 1100-1115页

作者： Maya P. bhatt Seema bhatt Birgit Gaye Institute for the Biogeochemistry and Marine Chemistry Klima Campus—University of Hamburg Hamburg Germany School of Environmental Science and Management Pokhara University Kathmandu Nepal

Surface water samples and lake bed sediments were collected and analyzed from Gosaikunda Lake within Langtang National Park (28°05'N, 85°25'E;4380 m a.s.l.) in the central Himalayan region of Nepal during fall 2011. The major cations and anions in equivalents were present in the following order: ?and , respectively. Sulfide oxidation coupled with carbonate dissolution and aluminosilicate dissolution appeared to be the dominant geochemical processes determining lake water dissolved ions. Sulfate concentration was much higher than the alkalinity which is in contrast to glacier meltwater within the same landscape. Alkalinity primarily as bicarbonate contributes 88.6% to the total dissolved inorganic carbon (DIC) followed by carbon dioxide (CO2) and carbonate (CO3) in surface water samples. Organic carbon contributes 0.3% to 5.4% to the sediments and the organic matter is predominantly of aquatic origin. The lake is under saturated with carbon dioxide and the average partial pressure of carbon dioxide (pCO2) appeared quite low (43.4 μatm). Overall, natural biogeochemical processes regulate the chemical species within the lake ecosystem. The lake is oligotrophic, however, nutrients and dissolved organic carbon (DOC) concentrations are enhanced at the near shore sites close to the tracking trail.

关键词： Gosaikunda High Himalaya Langtang Geochemical Processes Nutrients

Optimization of enzymatic hydrolysis of pigeon pea for cooking quality of dhal

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International Journal of Agricultural and Biological Engineering 2014年第5期7卷 123-132页

作者： V.P.Sangani N.C.Patel V.M.bhatt P.R.Davara D.K.Antala Department of Processing and Food Engineering College of Agricultural Engineering and TechnologyJunagadh Agricultural UniversityJunagadhGujaratIndia Anand Agricultural University AnandGujaratIndia

Cooking quality of dehusked splits is influenced by the dehulling method,in particular,by the pre-milling *** effects of four enzymatic hydrolysis parameters,i.e.,enzyme concentration(20-60 mg/100 g dry matter),incubation time(3-15 h),incubation temperature(40-60℃)and tempering water pH(4.0-6.0)on cooking time of pigeon pea dhal were optimized using response surface *** kinds of enzymes,i.e.,xylanase,pectinase,and cellulas were used in combination for enzymatic pre-treatment.A quadratic model satisfactorily described the dehulling efficiency with high value for the coefficient of determination R^(2)(0.9062).It predicted a minimum cooking time of 21.91 min at enzyme concentration of 37.8 mg/100 g dry matter,incubation time 8.69 min,incubation temperature 48.5℃ and pH 5.49 of tempering *** time at optimum condition was observed to be 21.50 min and the predicted values of cooking time showed 2.19% deviation from the experimental *** of the study revealed that cooking time of enzyme treated dhal could be decreased by 19.77% compared to the oil treated dhal.

关键词： pigeon pea enzymatic hydrolysis cooking quality enzymatic pre-treatment dehulling response surface methodology

Magnetic anchor guidance for endoscopic submucosal dissection and other endoscopic procedures

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World Journal of Gastroenterology 2017年第16期23卷 2883-2890页

作者： Mohamed Mortagy Neal Mehta Mansour A Parsi Seiichiro Abe Tyler Stevens John J Vargo Yutaka Saito Amit bhatt Department of Internal Medicine Homer Stryker MD School of MedicineWestern Michigan UniversityKalamazooMI 49008United States Department of Internal Medicine Medicine InstituteCleveland ClinicClevelandOH 44195United States Department of Gastroenterology and Hepatology Digestive Disease InstituteCleveland ClinicClevelandOH 44195United States Endoscopy Division National Cancer Center HospitalTokyo 104-0045Japan

Endoscopic submucosal dissection(ESD) is a wellestablished, minimally invasive treatment for superficial neoplasms of the gastrointestinal tract. The universal adoption of ESD has been limited by its slow learning curve, long procedure times, and high risk of complications. One technical challenge is the lack of a second hand that can provide traction, as in conventional surgery. Reliable tissue retraction that exposes the submucosal plane of dissection would allow for safer and more efficient dissection. Magnetic anchor guided endoscopic submucosal dissection(MAGESD) has potential benefits compared to other current traction methods. MAG-ESD offers dynamic tissue retraction independent of the endoscope mimicking a surgeon's "second hand". Two types of magnets can be used: electromagnets and permanent magnets. In this article we review the MAG-ESD technology, published work and studies of magnets in ESD. We also review the use of magnetic anchor guidance systems in natural orifice transluminal endoscopic surgery and the idea of magnetic non-contact retraction using surface ferromagentization. We discuss the current limitations, the future potential of MAG-ESD and the developments needed for adoption of this technology.

关键词： Endoscopic submucosal dissection Gastric cancer Magnetic anchor guidance Esophageal cancer Magnets Traction Natural orifice transluminal endoscopic surgery

变时滞不确定Lurie系统的时滞分布依赖鲁棒稳定性分析(英文)

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自动化学报 2012年第7期38卷 1100-1106页

作者： MUKHIJA Pankaj KAR Indra Narayan bhatt Rajendra Kumar Purushottam Department of Electrical Engineering Indian Institute of Technology Delhi

In this paper, the problem of robust absolute stability of Lurie system with probabilistic time-varying delay and normbounded parametric uncertainty is considered. The time delay variation range is divided into two sub-intervals. By considering the probability distribution of the time-varying delay between the two sub-intervals and the knowledge of the delay variation range, a novel linear matrix inequalities (LMIs) based stability condition is derived by defining a Lyapunov Krasovskii functional. It is illustrated with the help of numerical examples that the derived stability criteria can lead to less conservative results as compared to the results obtained by the traditional method of using the delay variation range information only.

关键词： Lurie系统时间延迟鲁棒稳定性分析时变时滞 Lyapunov泛函鲁棒绝对稳定性线性矩阵不等式参数不确定性

Clinical Significance of Liver Function Abnormality in Patients with COVID-19:A Single-center Experience from Western India

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Journal of Clinical and Translational Hepatology 2021年第6期9卷 878-888页

作者： Chetan R.Kalal harshad Joshi Vivek Kumar Divya Gopal Darshana Rathod Ashish Shukla Tarang Gianchandani Chetan bhatt Department of Gastroenterology Hepatology and Liver TransplantSir HN Reliance Foundation Hospital and Research Cen-treMumbaiMaharashtraIndia Department of Critical Care Sir HN Reliance Foundation Hospital and Research CentreMumbaiMaharashtraIndia Department of Administration Sir HN Reliance Foundation Hospital and Research CentreMumbaiMaharashtraIndia

Background and Aims:The impact of coronavirus disease-2019(COVID-19)on liver function remains to be fully *** study was designed to investigate such and determine the clinical significance in determining mortality ***:A retrospective study was conducted in patients with COVID-19 from March 2020 to July *** details were retrieved from electronic medical records to obtain clinical characteristics,medical history,laboratory tests,therapeutic intervention,and outcome ***:A total of 184 patients with COVID-19 were included(median age:45.5 years),comprised of 62.5%*** total,22(12.0%)patients had severe infection and 162(88.0%)had mild to moderate ***,95(51.6%)showed abnormal liver function test(LFT)and 17(9.2%)showed normal LFT at *** median age,hospital stay,and LFT were significantly higher in severe ***-severe infection(p<0.001).Out of 12 deaths,the majority were due to severe infection(n=11).Deaths were also due to acute respiratory distress syndrome(n=5),cardiac reasons(n=3),and sepsis with multiorgan failure(n=3).The median age,hospital stay and number of intensive care unit admissions were higher in patients having abnormal LFT compared to normal *** of elevated aspartate aminotransferase(42.8%and 40.4%),alanine transaminase(43.7%and 41.6%),and hypoalbuminemia(71.4%and72.7%)at admission and discharge were more common in severe *** mean survival was significantly lower in severe infection compared to those with non-severe disease(17.2 vs.52.3 days;p<0.001).Conclusions:Incidence of abnormal liver function was higher in patients with severe COVID-19 and was associated with prolonged hospital stay;mortality was associated with severity of *** ruling out the risk of liver injury,it is crucial to vigilantly monitor the liver function parameters in patients with COVID-19 admitted to hospital.

关键词： ALT/AST Hospital stay Hypoalbuminemia Liver injury Severe disease