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cDNA Cloning of Heat Shock Protein Genes and Their Expression in an Indigenous Cryptic Species of the Whitefly Bemisia tabaci Complex from China

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Journal of Integrative Agriculture 2012年第2期11卷 293-302页

作者： YU hao wan fang-hao GUO Jian-ying State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100081 P.R. China Department of Entomology Henan Institute of Science and Technology Xinxiang 453003 P.R.China

Thermal adaptation plays a fundamental role in shaping the distribution and abundance of insects,and heat shock proteins（Hsps）play important roles in the temperature adaptation of various *** better understand the temperature tolerance of the indigenous ZHJ2-biotype of whitefly Bemisia tabaci species complex,we obtained complete cDNA sequences for hsp90,hsp70,and hsp20 and analyzed their expression profiles under different high temperature treatments by real-time quantitative polymerase chain *** high temperature tolerance of *** ZHJ2-biotype was determined by survival rate after exposure to different high temperatures for 1 *** results showed that after 41°C heat-shock treatment for 1 h,the survival rates of ZHJ2 adults declined significantly and the estimated temperature required to cause 50% mortality（LT50）is 42.85°C for 1 *** for onset（Ton）or maximal（Tmax）induction of hsps expression in *** ZHJ2-biotype were 35 and 39°C（or 41°C）.Compared with previous studies,indigenous ZHJ2-biotype exhibits lower heat temperature stress tolerance and Ton（or Tmax）than the invasive B-biotype.

关键词： Bemisia tabaci biological invasion thermotolerance indigenous and invasive biotypes heat shock protein (hsp)

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双壁波纹钢管混凝土构件短柱轴压力学性能

哈尔滨工业大学学报 2025年第1期57卷 56-64页

作者：路博崔燕方勇王玉银李豪哈尔滨工业大学土木工程学院哈尔滨150090 结构工程灾变与控制教育部重点实验室(哈尔滨工业大学) 哈尔滨150090 深圳市市政设计研究院有限公司广东深圳518029

为改善空心钢筋混凝土柱(HRC)的使用与受力性能,提出一种双壁波纹钢管混凝土(CFDCST)构件。本文共进行了4个大尺寸双壁波纹钢管混凝土短柱与2个空心钢筋混凝土短柱的轴压试验,关键试验参数为试件类型与波纹钢管类型。分析构件的破坏模式... 详细信息

为改善空心钢筋混凝土柱(HRC)的使用与受力性能,提出一种双壁波纹钢管混凝土(CFDCST)构件。本文共进行了4个大尺寸双壁波纹钢管混凝土短柱与2个空心钢筋混凝土短柱的轴压试验,关键试验参数为试件类型与波纹钢管类型。分析构件的破坏模式,对比不同参数下构件的承载力、峰值应变、延性系数等关键力学指标,获得荷载-波纹钢管应变关系曲线以揭示试件的约束机理并验证外波纹钢管类型的影响。试验结果表明:外波纹钢管可为夹层混凝土提供约束作用,且螺旋角越小,外波纹钢管的约束作用越强;内波纹钢管则主要为夹层混凝土内壁提供有效支撑,以保持截面的完整性。名义约束效应系数与空心率相近时,由于咬口滑移影响,大螺旋角双壁波纹钢管混凝土比小螺旋角双壁波纹钢管混凝土的峰值应力、峰值应变与延性系数分别降低15.5%、21.8%与16.7%。在总用钢量相近的情况下,小螺旋角双壁波纹钢管混凝土柱的承载力、峰值应变与延性系数分别比空心钢筋混凝土柱提高10.6%、36.2%与50.0%。基于试验结果,检验了3种典型承载力公式的适用性,并提出了相应设计建议。

关键词：组合结构空心钢筋混凝土波纹钢管双壁构件约束机理

Lifetime gains and patterns of accumulation and mobilization of nutrients in the synovigenic parasitoid female,Diglyphus isaea Walker(Hymenoptera:Eulophidae),feeding different diets

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Lifetime gains and patterns of accumulation and mobilization...

腹腔镜QM-C1型广泛性子宫切除术与腹腔镜广泛性子宫切除术的比较研究

第三届全国生物入侵大会

作者： Yi-bo Zhang~1,wan-xue Liu~(1*),Wei wang~1,fang-hao wan~1,Qiang Li~2 1.State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing,P.R.China 100193 2.Entomology Department,College of Plant Protection,Yunnan Agricultural University,Kunming,P.R. China 650201

In nature,parasitoids feed as adult to obtain and use nutrients for supplementing and/or replenishing some of its existing array of nutrient ***,when adults feed on food(host or non-host food),they enhance its fitness,such as increasing egg production or *** both or one of these fitness components are enhanced depends on the parasitoid *** the present study ovigeny index(OI) and impact of females’ fitness as well as physiological state on the reproductive strategies was investigated in the synovigenic parasitoid Diglyphus isaea fed on host and non-host *** of the two foods on fecundity and longevity of the parasitoid was *** results showed that *** is a strong synovigenic parasitoid with lower OI *** types of foods enhanced fecundity and prolonged longevity of the *** females fed on non-host food showed higher levels of gut sugar,body sugar and glycogen than those fed on host food,but level of lipid was higher in the host-fed *** assumed *** females to be lipogenetic with low rate of lipid metabolism to replenish the requirement of egg ***,parasitoid female might directly absorb lipids from the paralyzed host haemolymph by injecting a kind of venom.

关键词： Diglyphus isaea Walker Fecundity Longevity Lipogenesis Mortality Synovigenic parasitoid Ovigeny index

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中国微创外科杂志 2025年第1期25卷 14-20页

作者：黄浩焦燕韩芳华南理工大学附属第六医院佛山市南海区人民医院妇科佛山528000 佛山复星禅诚医院妇科佛山528000

目的比较腹腔镜QM-C1型保留盆腔自主神经的广泛性子宫切除术(laparoscopic nerve-sparing radical hysterectomy,LNSRH)与腹腔镜广泛性子宫切除术(laparoscopic radical hysterectomy,LRH)治疗早期宫颈癌术后早期膀胱、直肠功能恢复情况,并总结识别和保留盆腔自主神经的经验。方法从华南理工大学附属第六医院佛山市南海区人民医院和佛山复星禅诚医院回顾性收集2009年9月~2023年9月早期宫颈癌200例LNSRH和160例LRH资料。对比2组术后膀胱与直肠功能恢复情况。结果与LRH组相比,LNSRH组住院时间短[(9.3±2.2)d vs.(11.0±2.4)d,t=-7.688,P<0.001],术后解除尿管早[(7.9±1.2)d vs.(15.7±2.6)d,t=-39.023,P<0.001],尿潴留发生率低(5例vs.23例,χ^(2)=17.475,P<0.001),其他膀胱功能障碍症状(包括夜尿、排尿困难、尿失禁和尿频/尿急)少(6例vs.30例,χ^(2)=24.500,P<0.001),排气早[(24.7±7.1)h vs.(32.4±4.4)h,t=-13.165,P<0.001]。结论与传统LRH相比,LNSRH有效地降低早期宫颈癌患者术后膀胱与直肠功能障碍的风险,促进更快的功能恢复。

关键词：宫颈癌 Querleu-Morrow C1型手术腹腔镜保留盆腔自主神经的广泛性子宫切除术腹腔镜广泛性子宫切除术

Cross-species microsatellite markers for revealing population genetic structure of the woolly apple aphid Eriosoma lanigerum

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Cross-species microsatellite markers for revealing populatio...

Effects of Carbon-based Fertilizer on Soil Physical and Chemical Properties，Enzyme Activities and Microbial Communities in Maize Fields in Arid Regions

第三届全国生物入侵大会

作者： Qiang Wu~(1,2),fang-hao wan~(1*),Zhao-hui LI~2 1.State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection, Chinese Academy of Agricultural Sciences Beijing,100193,China 2.College of Plant Protection,Shandong Agricultural University,Taian,Shandong,271018,China

The woolly apple aphid,Eriosoma lanigerum(Hausmann)(Homoptera:Pemphigidae),is an important pest of apples worldwide,but the molecular resource of this species is very *** closely related to model organisms present the opportunity to efficiently apply molecular tools developed by a large research community to taxa with different ecological and evolutionary histories. We tested cross-species amplification of 21 loci from three closely related species of woolly apple aphid,Pemphigus obesinymphae,Pemphigus bursarius and Pemphigus spyrothecae.14 of all these primer pairs amplified PCR product in woolly apple aphid in at least one test,but five of them amplified more than one *** results show high cross-species amplification capacity in closely related species of these sets of microsatellite loci,which might be useful for large-scale population genetic studies at a minimum cost,and the proportion of the tested loci that would display polymorphism in the woolly apple aphid should be tested in the *** implies that cross-species application of known microsatellite loci is a highly promising source of codominant markers for population genetic and evolutionary studies in aphids,or even other species.

关键词： Microsatellite markers Cross-species amplification Pemphigidae Eriosoma lanigerum Population genetics

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Huanjing Kexue/Environmental Science 2025年第3期46卷 1905-1914页

作者： Liu, Wei-Fan wang, Xiao-Gang Liu, Ji-Li Wu, Na wan, Meng-Hu Ma, Feng-Lan Liu, hao College of Agriculture Ningxia University Yinchuan750021 China College of Ecology and Environment Ningxia University Yinchuan750021 China

This study investigates the impact of biochar-based fertilizer on soil environmental factors and microbial communities to address how biochar-based fertilizer can improve soil conditions in arid regions and promote sustainable agricultural development. To achieve these goals,maize"Xianyu 335"was selected as the test variety and a random block design was adopted to set up five treatments:CK(conventional fertilizer at 450 kg·hm-2),T1(biochar-based fertilizer at 1 125 kg·hm-2),T2(biochar-based fertilizer at 1 013 kg·hm-2),T3(biochar-based fertilizer at 900 kg·hm-2),and T4(biochar-based fertilizer at 788 kg·hm-2). Soil samples were collected from mature corn fields and analyzed for changes in soil physicochemical properties,soil enzyme activities,and microbial diversity under the different biochar-based fertilizer treatments. The results showed that: ① Compared with that using chemical fertilizer alone,the application of biochar-based fertilizer significantly reduced the soil bulk density,increased the soil porosity and field water capacity,and increased the soil nutrients and enzyme activities. The contents of organic carbon,total nitrogen,and total phosphorus in the T1 and T2 treatments were significantly increased by 13.68%-32.63%,25.00%-26.79%,and 35.00%-47.50%,respectively. The activities of urease,sucrase,and alkaline phosphatase were significantly increased by 49.04%-91.35%,48.88%-58.71%,and 20.69%-31.03%,respectively. ② The T3 and T4 treatments reduced the Chao1,Shannon,Simpson,Faith_pd,and Pielou_e indices of microorganisms. The dominant bacteria of each treatment were Proteobacteria,Actinobacteria,and Acidobacteria,with relative abundances ranging from 71.50%-86.66%. The T1 and T2 treatments significantly increased the relative abundance of Proteobacteria and Actinomycetes and decreased the relative abundance of Acidobacteria. No significant difference was observed in the relative abundance of amino acid metabolism,carbohydrate metabolism,energy metabolism,gen

关键词： Alkalinity

Combination therapy strategy based on selective internal radiation therapy as conversion therapy for inoperable giant hepatocellular carcinoma:A case report

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World Journal of Gastrointestinal Oncology 2025年第3期17卷 410-417页

作者： Ming-Zhi hao Hai-Lan Lin Yu-Bin Hu Qi-Zhong Chen Zhang-Xian Chen Lin-Bin Qiu Duan-Yu Lin Hui Zhang De-Chun Zheng Zhu-Ting fang Jing-Feng Liu Department of Tumor Interventional Radiology Clinical Ocology School of Fujian Medical UniversityFujian Cancer HospitalFuzhou 350014Fujian ProvinceChina Department of Oncology and Vascular Interventional Radiology Clinical Oncology School of Fujian Medical UniversityFuzhou 350014Fujian ProvinceChina Department of Nuclear Medicine Clinical Oncology School of Fujian Medical UniversityFuzhou 350014Fujian ProvinceChina Department of Hepatopancreatobiliary Surgical Oncology Clinical Oncology School of Fujian Medical UniversityFujian Cancer HospitalFuzhou 350014Fujian ProvinceChina Department of Radiology Clinical Oncology School of Fujian Medical UniversityFujian Cancer HospitalFuzhou 350014Fujian ProvinceChina Department of Oncology and Vascular Interventional Radiology Clinical Oncology School of Fujian Medical UniversityFujian Cancer HospitalFuzhou 350014Fujian ProvinceChina Department of Hepatobiliary Surgery Clinical Oncology School of Fujian Medical UniversityFujian Cancer HospitalFuzhou 350014Fujian ProvinceChina

BACKGROUND Hepatocellular carcinoma(HCC)has become a growing health concern *** invasion and high tumor burden are key factors limiting the curative effect of selective internal radiation therapy(SIRT).CASE SUMMARY This case study reports a 49-year-old woman who was diagnosed with China Liver Cancer Staging(CNLC)IIIa HCC and>15 cm tumor ***,due to insufficient future liver remnant and vascular invasion,the tumor was unresectable;however,radical hepatectomy was performed after successful conversion therapy with SIRT using yttrium-90(90Y)resin microspheres followed by hepatic arterial infusion chemotherapy(HAIC)with tyrosine kinase inhibitor(TKI)and anti-programmed death-1(PD-1)*** using 90Y resin microspheres was given by the right hepatic artery and chemoembolization was simultaneously performed in the tumor’s feeding vessels from the right diaphragmatic *** was followed every three weeks with lenvatinib and *** 4 months post-SIRT,the tumor was downstaged to CNLC Ib and the patient successfully underwent *** histopathological examination of the resected specimen showed extensive *** This case study provides evidence for an integrated treatment strategy combining SIRT and HAIC with TKI and anti-PD-1 antibodies for patients with large HCC and microvascular *** confirmatory trials are required in the future.

关键词： Selective internal radiation therapy Hepatic arterial infusion chemotherapy Yttrium-90 resin microspheres Hepatocellular carcinoma Conversion therapy Case report

Effects of temperature on the development and fecundity of Bemisia tabaci B-biotype

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Effects of temperature on the development and fecundity of B...

China launches the “IAS1000 Project”

第三届全国生物入侵大会

作者： Gang Wu ~(1,2),Jian-ying Guo~2,fang-hao wan~(2*) 1.College of Plant Sciences and Technology,Huazhong Agricultural University,Wuhan 430070, China 2.State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing 100081,PR China.

Bemisia tabaci(Gennadius)(Homoptera:Aleyrodidae) B-biotype is one of the most destructive invasive pests in agricultural *** B-biotype has spread into more than 20 provinces in China and causes serious losses in vegetables,fruits and *** is a critical environmental factor that may influence the individual development and fecundity of insect herbivore. In this experiment,the growth and fecundity of *** B-biotype fed on different temperature were *** results showed that temperature significantly affected the egg life-span,larval life-span and fecundity of *** *** egg life-span of *** B-biotype was occurred to delay along with the temperature ***,significantly longer egg life-span of *** B-biotype was observed after feeding on 36℃and 39℃compared with those on 29℃and 33℃.Significantly longer first larval life-span of *** B-biotype was found after feeding on 39℃compared with those on 29℃and 33℃.The first larval life-span of *** B-biotype was not occurred after feeding on 29℃and 33℃in the successive three *** significantly lower first larval life-span of *** B-biotype was observed in the third generation compared with the first generation fed on 36℃.Significantly longer second larval life-span of *** B-biotype was found after feeding on 36℃compared with those on 29℃and 33℃.No difference was observed in the successive three generation after feeding on 29℃,33℃and 36℃.Significantly longer third larval life-span of *** B-biotype was found after feeding on 36℃and 39℃compared with those on 29℃and 33℃.Significantly longer third larval life-span of *** B-biotype was found in the first generation compared with the latter two generation fed on 36℃.The fourth larval life-span of B. tabaci B-biotype was observed in the second and third generations after feeding on 36℃compared with those on 29℃and 33℃.Significantly lower fecundity of *** B-biotype was obs

关键词： Bemisia tabaci Temperature Development Fecundity Insect herbivore

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Journal of Integrative Agriculture 2018年第12期17卷 2840-2841页

作者： QIAN wan-qiang wan fang-hao Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences Institute of Plant Protection Chinese Academy of Agricultural Sciences

1. IAS1000 Project The rapid development of omics provides new technologies *** Genomics Institute at Shenzhen (CAAS-AGIS)andInstituteofPlantProtection,ChineseAcademyof AgriculturalSciences(CAAS-IPP)initiatedthe"IAS1000Project"-A genome project of 1 000 invasive alien species,and established the "IAS1000 Alliance" in Shenzhen, China,on November 14, *** deep-mining of omics data,theprojectaimsforbetterunderstandingtheecological processesandmolecularmechanismsofbiological invasion, and developing new technologies and products for prevention and management of invasive alien ***

关键词： IAS1000 Project Project China launches the