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Overexpression of Arabidopsis YUCCA6 in Potato Results in High-Auxin Developmental Phenotypes and Enhanced Resistance to Water Deficit

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Molecular Plant 2013年第2期6卷 337-349页

作者： j.ong Im Kim Dongwon Baek Hyeong Cheol Park Hyun j.n Chun Dong-Ha Oh Min Kyung Lee j.on-Yung Cha Woe-Yeon Kim Min Chul Kim Woo Sik Chung hans j. bohner Sang Yeol Lee Ray A. Bressan Shin-Woo Lee Dae-j.n Yun Department of Biochemistry Purdue University West Lafayette IN 47907 USA Division of Applied Life Science (BK21 and WCU Program) Plant Molecular Biology and Biotechnology Research Center Gyeongsang National UniversityJinju 660-701 Republic of Korea Department of Plant Biology University of Illinois at Urbana-Champaign Urbana IL USA Department of Agronomy and Medicinal Plant Resources Gyeongnam National University of Science and Technology Jinju 660-758 Korea Faculty of Science King Abdulaziz University Jeddah 21589 KSA Department of Horticulture Purdue University West Lafayette IN 47907 USA

Indole-3-acetic acid （IAA）, a maj.r plant auxin, is produced in both tryptophan-dependent and tryptophan-independent pathways. A maj.r pathway in Arabidopsis thaliana generates IAA in two reactions from tryptophan. Step one converts tryptophan to indole-3-pyruvic acid （IPA） by tryptophan aminotransferases followed by a rate-limiting step converting IPA to IAA catalyzed by YUCCA proteins. We identified eight putative StYUC （Solanum tuberosum YUCCA） genes whose deduced amino acid sequences share 50%-70% identity with those of Arabidopsis YUCCA proteins. All include canonical, conserved YUCCA sequences： FATGY motif, FMO signature sequence, and FAD-binding and NADP-binding sequences. In addition, five genes were found with -50% amino acid sequence identity to Arabidopsis trypto-phan aminotransferases. Transgenic potato （Solanum tuberosum cv. j.won） constitutively overexpressing Arabidopsis AtYUC6 displayed high-auxin phenotypes such as narrow downward-curled leaves, increased height, erect stature, and longevity. Transgenic potato plants overexpressing AtYUC6 showed enhanced drought tolerance based on reduced water loss. The phenotype was correlated with reduced levels of reactive oxygen species in leaves. The results suggest a func-tional YUCCA pathway of auxin biosynthesis in potato that may be exploited to alter plant responses to the environment.

关键词： Solanum tuberosum potato Arabidopsis thaliana yuc6-1D YUCCA, StYUCCA, StTAA, auxin, drought.

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High-resolution Induan-Olenekian boundary sequence in Chaohu, Anhui Province

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Science China Earth Sciences 2005年第3期48卷 291-297页

作者： hans j. hansen Geological Institute Copenhagen University Oster Voldgade 10 DK-1350 Copenhage Denmark

The West Pingdingshan Section in Chaohu, Southeast China’s Anhui Province is one of the potential GSSP candidates for the Induan-Olenekian boundary. Extensive study has been done on the Induan-Olenekian boundary strata at the section to meet the requirements of defining the GSSP precisely. This paper focuses on the boundary strata at the section to present a review of the main results achieved in recent years. The Induan-Olenekian boundary strata here cover an interval of about four meters at the West Pingdingshan Section, in which the key indices of defining the boundary and the chief transitional processes of some parameters at the boundary are included. The relationship between the boundaries defined by ammonoids and by conodonts is well clarified at the section. The magnetic polarity analysis shows that the boundary interval belongs to a normal polarity zone. Both the carbonate and organic carbon isotopes com- positions experienced a significant conversion in the boundary interval.

关键词： Lower Triassic, Induan-Olenekian boundary, GSSP, candidate, Chaohu of China.

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Micromechanical investigation of granular ratcheting using a discrete model of polygonal particles

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Particuology 2008年第6期6卷 390-403页

作者： Fernando Alonso-Marroquín hans B. Mhlhaus hans j. Herrmann Earth Systems Science Computational Centre & School of Physical Sciences The University of Queensland Earth Systems Science Computational Centre The University of Queensland Institut fr Baustoffe ETH-HenggerbergZrich

We use a two-dimensional model of polygonal particles to investigate granular ratcheting. Ratcheting is a long-term response of granular materials under cyclic loading, where the same amount of permanent deformation is accumulated after each cycle. We report on ratcheting for low frequencies and extremely small loading amplitudes. The evolution of the sub-network of sliding contacts allows us to understand the micromechanics of ratcheting. We show that the contact network evolves almost periodically under cyclic loading as the sub-network of the sliding contacts reaches different stages of anisotropy in each cycle. Sliding contacts lead to a monotonic accumulation of permanent deformation per cycle in each particle. The distribution of these deformations appears to be correlated in form of vortices inside the granular assembly.

关键词： Granular systems Dynamics and kinematics of rigid bodies Molecular dynamics methods

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Modeling slow deformation of polygonal particles using DEM

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Particuology 2008年第6期6卷 506-514页

作者： Andrés A. Pea Pedro G. Lind hans j. Herrmann Institute for Computational Physics Universitt Stuttgart Computational Physics IfB HIF E12 ETH Hnggerberg CH-8093 Zrich Switzerland Departamento de Física Universidade Federal do Ceará

We introduce two improvements in the numerical scheme to simulate collision and slow shearing of irregular particles. First, we propose an alternative approach based on simple relations to compute the frictional contact forces. The approach improves efficiency and accuracy of the Discrete Element Method （DEM） when modeling the dynamics of the granular packing. We determine the proper upper limit for the integration step in the standard numerical scheme using a wide range of material parameters. To this end, we study the kinetic energy decay in a stress controlled test between two particles. Second, we show that the usual way of defining the contact plane between two polygonal particles is, in general, not unique which leads to discontinuities in the direction of the contact plane while particles move. To solve this drawback, we introduce an accurate definition for the contact plane based on the shape of the overlap area between touching particles, which evolves continuously in time.

关键词： Granular media Discrete element method Slow deformation Contact forces

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Enhancing Immune Responses for Cancer Therapy

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Cellular & Molecular Immunology 2007年第3期4卷 173-184页

作者： hans j.Stauss Department of Immunology and Molecular Pathology University College London Hampstead Campus Royal Free Hospital Rowland Hill Street London NW3 2PF United Kingdom

Although the immune system possesses the means to respond to cancer, it often fails to control the spread of malignancy. Nonetheless, equipping endogenous immunity to release a strong antitumor response has significant advantages over conventional therapies. This review explores some of the options available to accomplish this, focusing first on vaccinations with tumor antigens to stimulate the immune system and empower stronger antitumor responses. We then compare and contrast the so-far limited clinical success of vaccination with the well-documented curative potential of adoptive therapy using T lymphocytes transfer. Finally, we highlight novel approaches using T call receptor （TCR） gene transfer strategy to exploit allogeneic T cell repertoires in conj.nction with receptors selected in vitro for defined MHC/peptide combinations, as a basis for antigen-specific gene therapy of cancers. Cellular ＆ Molecular Immunology.

关键词： tumor antigen vaccination adoptive immunotherapy T cell receptor gene transfer TCR gene therapy

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Evolution and stability of holographic soliton pairs

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Chinese Science Bulletin 2006年第10期51卷 1174-1179页

作者： LIU j.nsong WANG Cheng ZHANG Huilan hans j. Eichler State Key Laboratory of Laser Technology Huazhong University of Science and Technology Wuhan 430074 China Optisches Institut Technische Universitaet Berlin Strasse des 17. Juni 135 D-10623 Berlin Germany

The dynamical evolution and the stabiity property of both bright and dark holographic solton pairs have been investigated numerically. Resultshow that mutual trapping between two beams in pair is balanceable and both ... 详细信息

关键词：空间光孤子非线性光学稳定性全息光学

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Anisotropic plastic deformation behavior of as-extruded ZK60 magnesium alloy at room temperature

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Science China(Technological Sciences) 2009年第1期52卷 161-165页

作者： hans j.R Department of Materials Science and Engineering Norwegian University of Science and Technology

The anisotropic plastic deformation behavior of as-extruded ZK60 magnesium alloy at room tempera-ture (RT) was investigated by compressive and tensile testing in different directions, i.e. the loading axis oriented at 0°, 45° and 90° to the extrusion direction. The relationship between texture and plastic deformation behavior were examined. The results show that the extruded ZK60 alloy exhibits a strong ring fiber texture. The mechanical properties are strongly orientation dependent. In tension testing, the 0° specimen exhibited higher yield strength and lower elongation. In compression testing, however, ZK60 alloy exhibited almost the same yield strength in three directions. The anisotropic plastic defor-mation behavior is due to strong fiber texture and the lower symmetry of the hexagonal close packed (hcp) structure of ZK60 alloy. The correlation between texture and mechanical behaviour offers the possibility to improve the mechanical properties of magnesium alloy by optimization of the material production process.

关键词： anisotropic plastic deformation behavior ZK60 Mg alloy texture mechanical property

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Recycling of automotive aluminum

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Transactions of Nonferrous Metals Society of China 2010年第11期20卷 2057-2063页

作者： j.rang CUI hans j.ROVEN Department of Materials Science and Engineering Norwegian University of Science and Technology(NTNU)

With the global warming of concern,the secondary aluminum stream is becoming an even more important component of aluminum production and is attractive because of its economic and environmental *** this work,recycling of automotive aluminum is reviewed to highlight environmental benefits of aluminum recycling,use of aluminum alloys in automotive applications,automotive recycling process,and new technologies in aluminum scrap *** survey shows that newly developed techniques such as laser induced breakdown spectroscopy(LIBS) and solid state recycling provide promising alternatives in aluminum scrap *** with conventional remelting and subsequent refinement,solid state recycling utilizing compression and extrusion at room or moderate temperature can result in significant energy savings and higher metal yield.

关键词： recycling automotive aluminum sorting mechanical separation solid state recycling

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Estimation of Pool Boiling Heat Transfer Coefficients of Ethanol-Water Mixtures

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Chinese j.urnal of Chemical Engineering 1999年第2期7卷 95-103页

作者：谷俊杰 hans-j.rgBart Lehrstuhl fur Thermische Verfahrenstechnik Universitat Kaiserslautern67653 KaiserslauternGermany

Based on the analysis of the principles of heat and mass transfer from the liquid bulk to bubble surface during nucleation, a new estimation method to predict the heat transfer of the nucleate pool boiling in binary mixtures is proposed in this paper by using the heat and mass transfer analogy (Colburn analogy) at the bubble surface transfer layers. With the use of Thome's concept of boiling range, an approximate method is derived to realize the calculation. The predicted results by this method are in good agreement with the experimental data from different sources.

关键词： heat transfer boiling water-ethanol mixture estimation

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Characteristics of Nd：GdVO4 Laser with Different Nd-Doping Concentrations

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Chinese Physics Letters 2006年第8期23卷 2088-2091页

作者：张玲张春雨魏志义张治国 Stephan Strohmaier hans j. Eichle Beij.ng National Laboratory for Condensed Matter Physics Institute of Physics Chinese Academy of SciencesBeijing 100080 Optical Institute Technical University Berlin Strasse de 17 Juni 135 D-10623 Berlin Germany

We report the properties of a compact diode-pumped continuous-wave Nd：GdV04 laser with a linear cavity and different Nd-doped laser crystals. In a 0.2at.% Nd-doped Nd：GdVO4 laser, 1.54 W output laser power is achieved at 912nm wavelength with a slope efficiency of 24.8% at an absorbed pump power of 9.4W. With 0.3at.% Nd-doping concentration, we can obtain the either single-wavelength emission at 1064nm or 912nm or the dual-wavelength emission at 1064nm and 912nm by controlling the incident pump power. From an incident pump power of 11.6 W, the 1064nm emission between ^4Fa/2 and ^4I11/2 is suppressed completely by the 912nm emission between ^4Fa/2 and ^4I9/2. We obtain 670 mW output of the 912nm single-wavelength laser emission with a slope efficiency of 5.5% by taking an incident pump power of 18.4 W. Using a Nd：GdV04 laser with 0.4at.% Nd-doping concentration, we obtain either the single-wavelength emission at 1064nm or the dual-wavelength emission at both 1064nm and 912nm by increasing the incident pump power. We observe a strong competition process in the dualavelength laser.

关键词： Nd:GdVO4 Laser Different Nd-Doping Concentrations

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