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SV2B defines a subpopulation of synaptic vesicles

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Journal of Molecular Cell Biology 2023年第9期15卷 1-13页

作者： Isabelle Paulussen hannes Beckert Timothy FMusial Lena J.Gschossmann Julia Wolf Mathieu Schmitt Jérôme Clasadonte Georges Mairet-Coello Christian Wolff Susanne Schoch Dirk Dietrich Synaptic Neuroscience Team Department of NeurosurgeryUniversity Hospital BonnBonn 53127Germany Synaptic Neuroscience Team Department of NeuropathologyUniversity Hospital BonnBonn 53127Germany Microscopy Core Facility Medical FacultyUniversity of BonnBonn 53127Germany UCB Pharma Braine l’Alleud 1420Belgium

Synaptic vesicles can undergo several modes of exocytosis,endocytosis,and trafficking within individual synapses,and their fates may be linked to different vesicular protein ***,we mapped the intrasynaptic distribution of the synaptic vesicle proteins SV2B and SV2A in glutamatergic synapses of the hippocampus using three-dimensional electron ***2B was almost completely absent from docked vesicles and a distinct cluster of vesicles found near the active *** contrast,SV2A was found in all domains of the synapse and was slightly enriched near the active ***2B and SV2A were found on the membrane in the peri-active zone,suggesting the recycling from both clusters of ***2B knockout mice displayed an increased seizure induction threshold only in a model employing high-frequency *** data show that glutamatergic synapses generate molecularly distinct populations of synaptic vesicles and are able to maintain them at steep spatial *** almost complete absence of SV2B from vesicles at the active zone of wildtype mice may explain why SV2A has been found more important for vesicle release.

关键词： 3D electron microscopy synaptic vesicles synaptic vesicle proteins endocytosis trafficking spatial organization

脆弱生态区生态系统服务消费与生态补偿研究:进展与挑战

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资源科学 2010年第5期32卷 797-803页

作者：甄霖刘雪林李芬魏云洁 hannes Koenig 中国科学院地理科学与资源研究所北京100101 中国科学院研究生院北京100049 德国莱布尼兹农业景观研究中心柏林慕钦堡15374

人类社会对生态系统服务的需求和消费日益增加,对生态系统状态和生态系统服务的可持续供给产生了深刻影响。人类采取了一系列响应措施来维持生态系统的健康,其中通过生态补偿的途径来保护生态系统成为当前学术界关注的热点。本文的目的... 详细信息

人类社会对生态系统服务的需求和消费日益增加,对生态系统状态和生态系统服务的可持续供给产生了深刻影响。人类采取了一系列响应措施来维持生态系统的健康,其中通过生态补偿的途径来保护生态系统成为当前学术界关注的热点。本文的目的是结合脆弱生态区的研究结果,对近年学术界在生态系统服务消费和生态补偿研究领域实质性进展进行论述,提出相关研究面临的挑战。分析表明,生态系统服务消费在理论研究的基础上,正在向消费行为、消费方式、消费效用,以及对环境和社会经济综合影响的定量化研究发展;生态补偿研究在补偿标准厘定方面有了重要进展,利益相关者分析方法的应用使得补偿主客体界定更具科学性。但是,应当充分重视相关研究面临的挑战和亟待解决的问题,为生态系统保护和满足人类福祉需求提供保障。

关键词：生态系统服务消费生态补偿消费效用利益相关者分析生态足迹

Serendipity in anticancer drug discovery

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World Journal of Clinical Oncology 2012年第1期3卷 1-6页

作者： Emily Hargrave-Thomas Bo Yu Jóhannes Reynisson Auckland Bioengineering Institute The University of Auckland Auckland 1142 New Zealand School of Chemical Sciences The University of Auckland Auckland 1142 New Zealand

It was found that the discovery of 5.8%(84/1437) of all drugs on the market involved serendipity. Of these drugs, 31(2.2%) were discovered following an incident in the laboratory and 53(3.7%) were discovered in a clinical setting. In addition, 263(18.3%) of the pharmaceuticals in clinical use today are chemical derivatives of the drugs discovered with the aid of serendipity. Therefore, in total, 24.1%(347/1437) of marketed drugs can be directly traced to serendipitous events confirming the importance of this elusive phenomenon. In the case of anticancer drugs, 35.2%(31/88) can be attributed to a serendipitous event, which is somewhat larger than for all drugs. The therapeutic field that has benefited the most from serendipity are central nervous system active drugs reflecting the difficulty in designing compounds to pass the blood-brain-barrier and the lack of laboratory-based assays for many of the diseases of the mind.

关键词： Anticancer drugs Drug discovery and development Serendipity

Can a Subcapital Osteotomy Fixed with an Intramedullary Locking Plate Correct all Possible Deformities of a Mild to Moderate Hallux Valgus?

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Open Journal of Orthopedics 2017年第9期7卷 254-283页

作者： Michael Vitek hannes Kugler Felix Fink Ornella Vitek Center for Foot and Joint Surgery Vienna Vienna Austria

Background: There are at least 5 very popular methods described for the correction of mild to moderate hallux valgus deformities. Despite of reported and self experienced good results in favorable fitting preoperative anatomical conditions of some of these methods;it seems that no one is really satisfying under “difficult” or non favorable preoperative anatomic conditions. How could an optimum operation method for a satisfying correction of a mild to moderate hallux valgus look like? The well known criteria for an optimally satisfying method to achieve full normalization of important forefoot parameters under all conditions should be a lateralization, plantarization and derotation of the Ist metatarsal head and also a normalization of the distal metatarsal articular angle (DMAA) in combination with a soft tissue correction. Methods: In order to achieve this outcome without the disadvantages of shortening or the need for unloading, we developed a new operation technique and new fixation devices. We performed a simple transverse lazy-L subcapital osteotomy and after an additional soft tissue release the Ist metatarsal head was restored to its preplaned optimum position in terms of narrowing the IM angle, plantarization, correction of the hallux valgus angle, derotation of a pronation l malrotation and improvement of the DMAA. Fixation was achieved with an intramedullary angel-stable transfixed 30 mm titanium plate. The rigidity of the implant rendered interfragmental compression or solid bone contact unnecessary. The goal was only an optimal correction. All patients were allowed to ambulate with full weightbearing immediately after the operation. Results: We retrospectively reviewed 346 patients (433 feet) who underwent subcapital osteotomy between May 2007 and December 2011;308 were women and 38 men;their mean age was 65 years. The follow-up investigation was performed on average after 6.5 years (78 months);range 5.5 to 10 years (66 to 120 months). The AOFAS Score im

关键词： Hallux Valgus Mild Moderate Toe Deformity Lateralization Plantarization Derotation Improvement of DMAA Full Weightbearing Hallux Valgus Surgery New Access Lazy-L Osteotomy Soft Tissue Correction IM-Angle Angle-Stable Locking Screws Intramedullary Locking Plate

EEG/ERP evidence of possible hyperexcitability in older adults with elevated beta-amyloid

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Translational Neurodegeneration 2022年第1期11卷 835-844页

作者： hannes Devos Kathleen Gustafson Ke Liao Pedram Ahmadnezhad Bradley Estes Laura E.Martin Jonathan D.Mahnken William M.Brooks Jeffrey M.Burns Department of Physical Therapy Rehabilitation Scienceand Athletic TrainingUniversity of Kansas Medical CenterKansas CityKS 66160USA Department of Neurology University of Kansas Medical CenterKansas CityKS 66160USA Hoglund Biomedical Imaging Center University of Kansas Medical CenterKansas CityKS 66160USA Department of Population Health University of Kansas Medical CenterKansas CityKS 66160USA Department of Biostatistics and Data Science University of Kansas Medical CenterKansas CityKS 66160USA University of Kansas Alzheimer’s Disease Center University of Kansas Medical CenterKansas CityKS 66160USA.

Background: Although growing evidence links beta-amyloid (Aβ) and neuronal hyperexcitability in preclinical mouse models of Alzheimer’s disease (AD), a similar association in humans is yet to be established. The first aim of the study was to determine the association between elevated Aβ (Aβ+) and cognitive processes measured by the P3 event-related potential (ERP) in cognitively normal (CN) older adults. The second aim was to compare the event- related power between CNAβ+ and CNAβ−. Methods: Seventeen CNAβ+ participants (age: 73 ± 5, 11 females, Montreal Cognitive Assessment [MoCA] score 26 ± 2) and 17 CNAβ- participants group-matched for age, sex, and MOCA completed a working memory task (n-back with n = 0, 1, 2) test while wearing a 256-channel electro-encephalography net. P3 peak amplitude and latency of the target, nontarget and task difference effect (nontarget−target), and event-related power in the delta, theta, alpha, and beta bands, extracted from Fz, Cz, and Pz, were compared between groups using linear mixed models. P3 ampli-tude of the task difference effect at Fz and event-related power in the delta band were considered main outcomes. Correlations of mean Aβ standard uptake value ratios (SUVR) using positron emission tomography with P3 amplitude and latency of the task difference effect were analyzed using Pearson Correlation Coefficient r. Results: The P3 peak amplitude of the task difference effect at Fz was lower in the CNAβ+ group (P = 0.048). Simi-larly, power was lower in the delta band for nontargets at Fz in the CNAβ+ participants (P = 0.04). The CNAβ+ partici-pants also demonstrated higher theta and alpha power in channels at Cz and Pz, but no changes in P3 ERP. Strong correlations were found between the mean Aβ SUVR and the latency of the 1-back (r = − 0.69;P = 0.003) and 2-back (r = − 0.69;P = 0.004) of the task difference effect at channel Fz in the CNAβ+ group. Conclusions: Our data suggest that the elevated amyloid in cognitively normal ol

关键词： Event-related potentials Electro-encephalography Working memory Older adults Preclinical Beta-amyloid

马格里布—欧洲输气管道使用三层PE覆盖层系统

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油气储运 1996年第8期15卷 49-50页

作者： Jo hannes Haimbl 龚弋中国石油天然气管道勘察设计院

在马格里布—欧洲输气管道的摩洛哥段(长550km),采用了直径为1220mm的三层PE外覆盖层和一层液体环氧内衬的管子。外覆盖层涂敷步骤,首先是管面符合要求后用静电喷涂溶结环氧粉末(FBE)作底层,在FBE底涂料完全固化后涂敷粘合剂,再用侧挤... 详细信息

在马格里布—欧洲输气管道的摩洛哥段(长550km),采用了直径为1220mm的三层PE外覆盖层和一层液体环氧内衬的管子。外覆盖层涂敷步骤,首先是管面符合要求后用静电喷涂溶结环氧粉末(FBE)作底层,在FBE底涂料完全固化后涂敷粘合剂,再用侧挤工艺涂敷顶层聚乙烯。三层PE覆盖层系统具有突出的抗机械性和粘结性,还具有较高的电阻,这将大大降低阴极保护系统的装置和维护费用。

关键词：输气管道涂层环氧粉末防护

Lymph Fistulas after Inguinal Lymph Node Dissection—Assessment of Risk Factors and Future Treatment Strategies

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Surgical Science 2014年第4期5卷 193-199页

作者： Nils Habbe Alexander Reinisch Wolf Otto Bechstein Sabine hannes Department of General and Visceral Surgery University Hospital Frankfurt Frankfurt Germany

Background: Inguinal lymph node dissections (ILND) have been commonly associated with postoperative complications such as lymphedema, lymph fistula and wound infections. The reported complication rates after ILND range from 14% - 77%, and a rationale for these large differences is the use of a variety of different definitions of complications. Patients and Methods: Files of patients that underwent inguinal lymph node dissection for lymph node metastases at the Department of General and Visceral Surgery of the University Hospital Frankfurt between February 2005 and March 2012 were retrospectively reviewed. Results: 47 patients (65%) developed a lymphatic fistula in the course of the operation, of which 6 patients (8%) underwent reoperations. Surgical side infections (SSI) were seen in 15 patients (21%), all of them harbouring fistulas. Patients presenting with a BMI ≥ 30 kg/m2 did not develop more fistulas than those with a BMI 2 (p = 0.30). Patients who suffered from a lymph fistula had significantly more visits in the outpatient clinic (p =< 0.0001). There was no difference in the complication rate if the procedure was performed by a resident under supervision or a senior surgeon (p = 0.79). Conclusion: In this study, we demonstrated that lymph fistulas are to date a common complication after ILND and lead to reoperations and significantly more outpatient visits. The number of lymph nodes affected and resected, and prior sentinel lymph node dissection, was not associated with a higher complication rate.

关键词： Lymph Fistula Inguinal Lymph Node Lymphadenectomy Wound Infection

Poultry By-Product Meals as Partial Fish Meal Replacement Yielded Higher Somatic Growth in Alsatian Charr (<i>Salvelinus alpinus X fontinalis</i>) than Pork or Vegetable-Based Fish Meals

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Open Journal of Animal Sciences 2018年第3期8卷 191-205页

作者： Martin J. Kainz hannes H. Hager Eduard Schneeberger WasserCluster—Biologische Station Lunz Lunz am See Austria GARANT Tiernahrnung Pochlarn Austria

Freshwater salmonids gain increasing popularity as diet fish and are an important dietary source of omega-3 polyunsaturated fatty acids (PUFA) for humans. The aim of this study was to investigate effects of alternative feeds from domestic sources on fish growth and lipid composition of the sparctic Alsatian charr (Salvelinus alpinus X fontinalis), commonly used in freshwater aquaculture in Europe. In this fish feeding experiment, we used a control feed (32% fish meal;feed F1) and experimental diets containing generally only half the amount of fish meal, which was replaced in equal parts by poultry (F2), two different pork by-product meals (F3 and F4), plant-based feeds (F5), or by a feed containing 25% fish and 15% poultry meal (F6) that had a similar caloric value compared to the other feeds. Six hundred charrs of similar initial weight (ca. 90 g) were randomly distributed into 12 tanks (50/tank) of 1.4 m3, supplied with subalpine spring water, and fed one of the feeds. Fish biomass development was the highest in fish fed diet F6, followed by F1, F2, and F5. Pork by-product meals as partial replacement of fish meal resulted on average in a 25% lower biomass gain compared to charr feeding on poultry by-product meal (F6). The use of poultry or pork by-product meals as partial fish meal replacements did not significantly change the total lipids or fatty acids retained in these fish. This study shows that these alternative feeds, with similar lipid sources, had no significant effect on the fatty acid composition in Alsatian charr, however, poultry by-product meal as partial replacement of marine fish meal clearly enhanced charr biomass by 15% relative to conventional fish meals.

关键词： Alternative Protein Sources Feed Formulation Salmonids Muscle Lipids Fillet Composition

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中国土地利用多功能性动态的区域分析

生态学报 2010年第24期30卷 6749-6761页

作者：甄霖魏云洁谢高地 HELMING Katharina 曹淑艳杨莉潘影 KOENIG hannes 中国科学院地理科学与资源研究所北京100101 中国科学院研究生院北京100049 德国莱布尼兹农业景观研究中心柏林慕钦堡15374 北京石油化工学院北京102617

当前,国际学术界针对土地利用的研究正由土地利用格局变化向土地空间多功能变化及其可持续性的方向发展,其宗旨是确定和度量土地多元化利用所提供的产品、服务和功能,及其带来的环境和社会经济效应,实现对土地利用的科学管理。基于可持... 详细信息

当前,国际学术界针对土地利用的研究正由土地利用格局变化向土地空间多功能变化及其可持续性的方向发展,其宗旨是确定和度量土地多元化利用所提供的产品、服务和功能,及其带来的环境和社会经济效应,实现对土地利用的科学管理。基于可持续发展三维度的理念及其指标体系,界定并定量评价了我国区域土地利用的10项功能,深入研究了在经济快速发展、生态环境保护紧密相随的过去20a间,各项功能的时空变化特征。研究表明,我国土地多功能性的10项功能中,就业支持功能表现出区域增减不一的特点,生态过程维持功能具有高度的稳定性。健康保障、交通功能、居住家园、生物性土地生产、人工化土地生产、资源供给与维持、污染接收器、景观与文化支持功能在各个区域得到了不断改善与提高,但各个功能变化的强势区和弱势区在时空分布上表现出了很强的区域性特点。区域自然条件禀赋、社会经济条件以及土地利用政策在功能变化中起着关键作用。研究结果揭出中国区域土地多功能性尚存在巨大的提升空间;土地政策应具有系统性、功能针对性和时空针对性,并应关注土地利用功能变化的链发效应。

关键词：土地空间利用土地多功能性生态系统服务功能时空尺度中国