检索结果-南通市图书馆

Net energy,energy utilization,and nitrogen and energy balance affected by dietary pea supplementation in broilers

Animal Nutrition 2021年第2期7卷 506-511页

作者： Nishchal K.Sharma Zhibin Ban hank l.classen Huaming Yang Xiaogang Yan Mingan Choct Shu-Biao Wu School of Environmental and Rural Science University of New EnglandArmidaleNSW 2351Australia laboratory of Animal Nutrition Metabolism Jilin Academy of Agricultural SciencesGongzhulingJilin 136100China Department of Animal and Poultry Science University of Saskatchewan51 Campus DriveSaskatoonSK S7N 5A8Canada Office of the Pro Vice-Chancellor External Relations University of New EnglandArmidaleNSW 2351Australia

Pea starch consists predominantly of C-type of amylopectin chain which is more resistant to digestive enzymes than A-type of starch thus slowly digested in *** was hypothesized that the presence of slowly digested pea starch in broiler diets will increase net energy and the efficiency of energy utilization in *** experiments were performed to investigate starch digestibility of pea at different in-cubation times(in vitro study)and the effect of dietary pea on heat increment and net energy in broilers using an open-circuit respiratory calorimetry system(in vivo study).One-day-old Ross 308 male broilers were fed a common starter crumble from d 1 to 10 and standard grower diets *** d 21,birds were transferred to the chambers each housing 2 *** treatment was replicated 6 times with 2 identical runs of 3 replicates per treatment.A wheat-soybean meal-based diet was used as a control and the treatment diet contained 500 g of pea/kg *** vitro study showed that pellet processing increased(Plity,particularly at shorter times for wheat and a much larger response for *** offered the pea-based diet had lower(P=0.002)feed intake,lower(P=0.020)body weight gain,but a similar(P>0.05)FCR compared to those offered the wheat-based *** energy(NE)and apparent metabolizable energy(AME)values were higher in the pea-based diet than in the wheat-based diet(P-0.037 for NE and P-0.018 for AME).Heat production,respiratory quotient,heat increment of feed,efficiency of utilization of gross energy for AME,and efficiency of utilization of AME for NE did not differ(P>0.05)between the 2 *** was no effect(P>0.05)of pea on the total tract di-gestibilities of dry matter,crude protein and ash,but the total tract digestibility of starch was higher(P-0.022)in the pea-based diet compared to the wheat-based *** study provides insight into the energy metabolism of broilers offered a pea-based diet and indicates that dieta

关键词： Slowly digested starch Pea Heat increment Net energy Broiler

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

G-Quadruplex(G4) Motifs in the Maize(Zea mays l.) Genome Are Enriched at Specific locations in Thousands of Genes Coupled to Energy Status,Hypoxia,low Sugar,and Nutrient Deprivation

引用

Journal of Genetics and Genomics 2014年第12期41卷 627-647页

作者： Carson M.Andorf Mykhailo Kopylov Drena Dobbs Karen E.Koch M.Elizabeth Stroupe Carolyn J.lawrence hank W.Bass USDA-ARS Corn Insects and Crop Genetics Research Unit Iowa State University Department of Computer Science Iowa State University Institute of Molecular Biophysics Florida State University Department of Genetics Development and Cell BiologyIowa State University Plant Molecular and Cellular Biology Program Horticultural Sciences DepartmentGenetics InstituteUniversity of Florida Department of Biological Science Florida State University Department of Agronomy Iowa State University

The G-quadruplex （G4） elements comprise a class of nucleic acid structures formed by stacking of guanine base quartets in a quadruple helix. This （34 DNA can form within or across single-stranded DNA molecules and is mutually exclusive with duplex B-form DNA. The reversibility and structural diversity of G4s make them highly versatile genetic structures, as demonstrated by their roles in various functions including telomere metabolism, genome maintenance, immunoglobulin gene diversification, transcription, and translation. Sequence motifs capable of forming G4 DNA are typically located in telomere repeat DNA and other non-telomeric genomic loci. To investigate their potential roles in a large-genome model plant species, we computationaily identified 149,988 non-telomeric G4 motifs in maize （Zea mays l., B73 AGPv2）, 29% of which were in non-repetitive genomic regions. G4 motif hotspots exhibited non-random enrichment in genes at two locations on the antisense strand, one in the 5~ UTR and the other at the 5~ end of the first intron. Several genic G4 motifs were shown to adopt sequence-specific and potassium-dependent G4 DNA structures in vitro. The G4 motifs were prevalent in key regulatory genes associated with hypoxia （group VII ERFs）, oxidative stress （D J-1/GATasel）, and energy status （AMPK/ SnRK） pathways. They also showed statistical enrichment for genes in metabolic pathways that function in glycolysis, sugar degradation, inositol metabolism, and base excision repair. Collectively, the maize G4 motifs may represent conditional regulatory elements that can aid in energy status gene responses. Such a network of elements could provide a mechanistic basis for linking energy status signals to gene regulation in maize, a model genetic system and major world crop species for feed, food, and fuel.

关键词： Maize G-quadruplex G4 Hypoxia Sucrose synthase

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Clarifying the influence of albumin on the initial stages of magnesium corrosion in hank's balanced salt solution

引用

Journal of Magnesium and Alloys 2021年第3期9卷 805-817页

作者： Di Mei Cheng Wang Sviatlana V.lamaka Mikhail l.Zheludkevich Magnesium Innovation Centre-MagIC Institute of Materials ResearchHelmholtz-Zentrum GeesthachtGeesthacht 21502Germany Institute for Materials Science Faculty of EngineeringKiel UniversityKiel 24103Germany

The corrosion behavior of Mg,a promising biodegradable metallic material,in protein-containing pseudophysiological environment is still not fully *** this work,the influence of albumin on the corrosion behavior of commercially pure magnesium(CP Mg)is investigated during short-term tests in hank’s Balanced Salt Solution(HBSS).This work focuses on the reactions at the Mg/medium interface from the perspective of the interactions among albumin,media components and *** evolution tests demonstrate that the physiological amount of albumin(40 g l^(-1))accelerates Mg corrosion in HBSS during the first few hours but slows down the degradation *** presence of albumin decreases the concentration of free Ca^(2+)in HBSS and delays formation of protective co-precipitation products on Mg *** evolution of local pH(differs from typically monitored bulk pH)near Mg/medium interface in albumincontaining HBSS is reported for the first *** of local and bulk pH values elucidates the pH buffering effect of albumin during the immersion *** on these results,adsorption,chelating and pH buffering effects are summarized as three important aspects of albumin influence on Mg ***,constant replenishment of medium components is shown to be an influential factor during the Mg corrosion tests.

关键词： Magnesium Corrosion Albumin Biodegradable local pH

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Performance and calibration of quark/gluon-jet taggers using 140 fb^(-1) of pp collisions at √s=13 TeV with the ATlAS detector

引用

Chinese Physics C 2024年第2期48卷 1-25页

作者： G. Aad B. Abbott K. Abeling N.J. Abicht S.H. Abidi A. Aboulhorma H. Abramowicz H. Abreu Y. Abulaiti B.S. Acharya C. Adam Bourdarios l. Adamczyk S.V. Addepalli M.J. Addison J. Adelman A. Adiguzel T. Adye A.A. Affolder Y. Afik M.N. Agaras J. Agarwala A. Aggarwal C. Agheorghiesei A. Ahmad F. Ahmadov W.S. Ahmed S. Ahuja X. Ai G. Aielli A. Aikot M. Ait Tamlihat B. Aitbenchikh I. Aizenberg M. Akbiyik T.P.A. Åkesson A.V. Akimov D. Akiyama N.N. Akolkar K. Al Khoury G.l. Alberghi J. Albert P. Albicocco G.l. Albouy S. Alderweireldt M. Aleksa I.N. Aleksandrov C. Alexa T. Alexopoulos F. Alfonsi M. Algren M. Alhroob B. Ali H.M.J. Ali S. Ali S.W. Alibocus M. Aliev G. Alimonti W. Alkakhi C. Allaire B.M.M. Allbrooke J.F. Allen C.A. Allendes Flores P.P. Allport A. Aloisio F. Alonso C. Alpigiani M. Alvarez Estevez A. Alvarez Fernandez M. Alves Cardoso M.G. Alviggi M. Aly Y. Amaral Coutinho A. Ambler C. Amelung M. Amerl C.G. Ames D. Amidei S.P. Amor Dos Santos K.R. Amos V. Ananiev C. Anastopoulos T. Andeen J.K. Anders S.Y. Andrean A. Andreazza S. Angelidakis A. Angerami A.V. Anisenkov A. Annovi C. Antel M.T. Anthony E. Antipov M. Antonelli F. Anulli M. Aoki T. Aoki J.A. Aparisi Pozo M.A. Aparo l. Aperio Bella C. Appelt A. Apyan N. Aranzabal S.J. Arbiol Val C. Arcangeletti A.T.H. Arce E. Arena J-F. Arguin S. Argyropoulos J.-H. Arling O. Arnaez H. Arnold G. Artoni H. Asada K. Asai S. Asai N.A. Asbah J. Assahsah K. Assamagan R. Astalos S. Atashi R.J. Atkin M. Atkinson H. Atmani P.A. Atmasiddha K. Augsten S. Auricchio A.D. Auriol V.A. Austrup G. Avolio K. Axiotis G. Azuelos D. Babal H. Bachacou K. Bachas A. Bachiu F. Backman A. Badea P. Bagnaia M. Bahmani A.J. Bailey V.R. Bailey J.T. Baines l. Baines O.K. Baker E. Bakos D. Bakshi Gupta V. Balakrishnan R. Balasubramanian E.M. Baldin P. Balek E. Ballabene F. Balli l.M. Baltes W.K. Balunas J. Balz E. Banas M. Bandieramonte A. Bandyopadhyay S. Bansal l. Barak M. Barakat E.l. Barberio D. Barberis M. Barbero M.Z. Barel K.N. Barends T. Barillari M-S. Barisits T. Barklow P. Baron D.A. Baron Mo The ATlAS Collaboration

The identification of jets originating from quarks and gluons,often referred to as quark/gluon tagging,plays an important role in various analyses performed at the large Hadron Collider,as Standard Model measurements and searches for new particles decaying to quarks often rely on suppressing a large gluon-induced *** paper describes the measurement of the efficiencies of quark/gluon taggers developed within the ATlAS Collaboration,using √s=13 TeV proton–proton collision data with an integrated luminosity of 140 fb^(-1) collected by the ATlAS *** taggers with high performances in rejecting jets from gluon over jets from quarks are studied:one tagger is based on requirements on the number of inner-detector tracks associated with the jet,and the other combines several jet substructure observables using a boosted decision tree.A method is established to determine the quark/gluon fraction in data,by using quark/gluon-enriched subsamples defined by the jet *** in tagging efficiency between data and simulation are provided for jets with transverse momentum between 500 GeV and 2 TeV and for multiple tagger working points.

关键词： ATlAS JET QUARK GlUON TAGGING

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Diameter-dependent in vitro performance of biodegradable pure zinc wires for suture application

引用

Journal of Materials Science & Technology 2019年第8期35卷 1662-1670页

作者： H. Guo R.H. Cao Y.F. Zheng J. Bai F. Xue C.l. Chu State Key laboratory for Turbulence and Complex System and Department of Materials Science and Engineering College of Engineering Peking University Beijing 100871 China School of Materials Science and Engineering Southeast University Nanjing 211189 China Key laboratory for Advanced Metallic Materials Southeast University Nanjing 211189 China

In this study, biodegradable pure zinc wires with 3.0 mm and 0.3 mm in diameter were prepared via hot-extrusion and subsequent cold-drawing process respectively. The microstructure, mechanical performance, corrosion behavior, in vitro cytocompatibility and antibacterial effect were comparatively *** cold-drawing, the mechanical property, especially the elongation of the ф0.3 mm pure Zn wire was improved significantly compared with the ф3.0 mm pure Zn wire. The in vitro corrosion study including immersion and electrochemical test showed acceptable corrosion resistance of these two materials in hank's solution. The in vitro Human Umbilical Vein Endothelial Cells(HUVECs) viability assay showed obviously different results, in which the ф0.3 mm pure Zn wire demonstrated favorable cytocompatibility, while the ф3.0 mm wire exhibited severe cytotoxic effect with 100% extract concentration. Both of them exhibited partly antibacterial effect on S. aureus. These results demonstrated the feasibility of the prepared 0.3 mm pure Zn wire as the potential suture material with good absorbability.

关键词： Pure zinc Wire Biodegradable metal Suture

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

lC-MS/MS方法测定大鼠脑中9种非标记PET显像剂的浓度

LC-MS/MS方法测定大鼠脑中9种非标记PET显像剂的浓度

引用

2018年中国质谱学术大会（CMSC2018）

作者：孙明月肖好洪海燕张爱丽张岩刘亚静朱霖 hank F KUNG 乔晋萍北京师范大学化学学院首都医科大学脑重大疾病研究院

正电子发射计算机断层扫描(position emission tomography, PET)在临床疾病诊断和治疗监测中具有重要的作用,正电子放射性药物的研究与评价是PET技术发展的关键。采用lC-MS/MS技术对非放射性标记的PET药物进行高通量筛选是当前研究的热... 详细信息

正电子发射计算机断层扫描(position emission tomography, PET)在临床疾病诊断和治疗监测中具有重要的作用,正电子放射性药物的研究与评价是PET技术发展的关键。采用lC-MS/MS技术对非放射性标记的PET药物进行高通量筛选是当前研究的热点。本文建立了一种灵敏、高效的lC-MS/MS分析方法同时测定大鼠脑中9种非标记PET药物的浓度,该方法可用于筛选生物学性质优良的PET显像剂。1.实验部分1.1仪器与试剂质谱仪:Triple Quad 5500,美国AB SCIEX公司;高效液相色谱仪:1260 HPlC,美国Agilent公司。9种化合物由美国宾夕法尼亚大学***教授实验室提供(纯度>99.5%);丁苯那嗪(TBZ)为分析纯;乙腈、甲醇、醋酸铵为色谱纯;水为超纯水。1.2分析条件质谱条件电离模式ESI+;离子化电压5500 V;离子源温度550°C;喷雾气50 psi;辅助加热气45 psi;气帘气35 psi;碰撞气8 psi;扫描方式MRM。色谱条件Waters XTerra C18色谱柱(150 mm×2.1 mm, 5μm);进样量2μl;流速0.3 ml?min-1;流动相:A为水(含2 mM醋酸铵),B为甲醇(含2 mM醋酸铵);梯度洗脱条件为:0～2 min,B为10%;2～3.5 min,B从10%递增到98%;3.5～6 min,B为98%;6～6.01 min,B从98%到10%,6.01～8 min,B为10%。1.3方法学研究以大鼠脑组织匀浆为基质,配制标准样品,按照美国FDA颁发的"生物分析方法确证指南"进行方法学验证。考察了线性范围、最低定量限、重复性、回收率、日内精密度、日间精密度、准确度、基质效应等,9个化合物的分离度良好,所有方法学验证项目均满足要求,可以用于生物样品分析。2.结果与讨论本研究建立了定量分析大鼠脑中9种化合物的lC-MS/MS分析方法,该方法快速、灵敏、准确,专属性强,重复性好,能够用于9种非标记PET显像剂的筛选和评价研究。

关键词： PET 正电子放射性药物 lC-MS/MS

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Food Safety and Antimicrobial Resistance an Approach to the Genus <i>Salmonella</i>spp.

引用

Journal of Biosciences and Medicines 2017年第2期5卷 55-71页

作者： Alejandro de Jesú s Corté s-Sá nchez Raquel Garcia-Barrientos Hugo Minor-Pé rez Octavio Dublá n-Garcí a Alejandra l. San Martin-Azocar Secretaria de Salud Comisi&oacute n Federal para la Protecció n contra Riesgos Sanitarios Control Anal&iacute tico Departamento de Microbiolog&iacute a Calz. De Tlalpan 4492 Tlalpan Ciudad de M&eacute xico M&eacute xico lab de Procesos Biotecnoló gicos Universidad Polit&eacute cnica de Tlaxcala Av. Universidad Polit&eacute cnica No. 1 San Pedro Xalcaltzinco Tepeyanco Tlaxcala M&eacute xico Divisió n de Ingenierí a Quí mica y Bioquí mica Tecnol&oacute gico de Estudios Superiores de Ecatepec Av. Tecnol&oacute gico s/n Esq. Av. Carlos hank Gonzá lez (Av. Central) Col. Valle de An&aacute huac Ecatepec de Morelos Estado de M&eacute xico M&eacute xico Facultad de Quí mica Universidad Aut&oacute noma del Estado de Mé xico Avenida Paseo Tollocan S/N Toluca Estado de M&eacute xico M&eacute xico Instituto Tecnoló gico y de Estudios Superiores de Monterrey Escuela Superior de Ingenier&iacute a en Alimentos Biotecnolog&iacute a Agronom&iacute a. Av. Epigmenio Gonzá lez 500 Santiago de Quer&eacute taro M&eacute xico

Foodborne Illnesses (FI) are considered an important problem in public health for their high levels of morbidity and, in some cases, of mortality in Mexico and around the world. Different agents that cause Foodborne Illnesses, in which biological agents such as the genus Salmonella spp., are included, have been often associated with outbreaks. A continued effort has been observed in the food industry, in collaboration with sanitary authorities on a global scale, through the creation and continued improvement of different procedures to prevent the contamination by Salmonella, in which the elaboration of laboratory methodologies for the detection and isolation of this pathogen in foods and, in such manner, prevents the outbreak of illnesses. However, along with the aforementioned, it has been reported that a few years ago, the appearance of an ever-increasing number of strains of Salmonella spp., in foods with multi-drug resistance to antibiotics which are used in the treatment of its illness, results in a major emphasis on the health issue related to Foodborne Illnesses and, in particular, to those generated by factors of medical dependence such as the increase in recuperation time, costs, and reduction in the number of alternative pharmaceutical treatments. This work presents a general overview of FI, in which illnesses generated by bacteria of the genus Salmonella spp., their detection in a microbiological laboratory, as well as the phenomenon of resistance to antibiotics by these bacteria, and the current and difficult issue reported through different investigations all around the world, of the rise in this phenomenon and its importance in public health, are discussed.

关键词： Health Salmonellosis Salmonella Food Safety Antimicrobial Resistance

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

成体肝脏干细胞的体外分离、培养及鉴定(英文)

引用

中国组织工程研究与临床康复 2007年第50期11卷 10169-10172页

作者：吕国悦张平李红李爽王广义吉林大学第一医院普外科吉林省长春市130021 东丰县中医院普外科吉林省辽源市136300

背景:肝脏干细胞的多能性在肝脏移植方面具有重要价值。目前已经成功地鉴定并分离出动物肝脏干细胞,但人肝脏组织是否存在干细胞、如何对其进行分离鉴定的报道不多。目的:尝试从人类肝癌旁组织中分离和体外培养肝脏干细胞,并对其表面标... 详细信息

背景:肝脏干细胞的多能性在肝脏移植方面具有重要价值。目前已经成功地鉴定并分离出动物肝脏干细胞,但人肝脏组织是否存在干细胞、如何对其进行分离鉴定的报道不多。目的:尝试从人类肝癌旁组织中分离和体外培养肝脏干细胞,并对其表面标志物进行鉴定,寻找肝脏干细胞新的来源。设计:细胞观察实验。单位:吉林大学第一医院普外科,东丰县中医院普外科。材料:①组织来源:10例标本来自2005-10/2006-06吉林大学第一临床学院肝胆外科收治的肝细胞癌患者,年龄45～58岁,在行肝叶切除术时切取距癌巢边2cm处的肝组织,经病理证实无癌细胞,对本实验均签署知情同意书。②主要试剂:细胞培养用DMEM/F12干粉由美国Hyclone公司提供,新鲜胎牛血清由天津联星生物技术有限公司配制,各种细胞生长因子为美国Cytolay公司产品。方法:将肝脏癌旁组织剪碎,hank’s液冲洗,Ⅳ型胶原酶溶液消化后将细胞悬浮在含体积分数为0.15胎牛血清的DMEM/F12培养液中,加入肝细胞生长因子、表皮生长因子、α-成纤维细胞生长因子,浓度均为25μg/l。待培养细胞约铺满2/3瓶底后,胰蛋白酶消化传代,按2×10~7l^(-1)密度接种继续培养。细胞传至3～4代时,加入胰酶制成浓度为2.60×10~9l^(-1)细胞悬液,依次加入抗人C-kit抗体、免疫磁珠、Buffer液,免疫磁珠法初步分离C-kit^+细胞。HE染色和免疫荧光细胞化学染色检测癌旁组织中是否存在肝脏干细胞。制备C-kit^+细胞爬片,免疫荧光化学染色鉴定肝脏干细胞表面标志物CK19及AFP的表达。主要观察指标:①细胞形态观察。②癌旁组织肝脏干细胞的鉴定。③C-kit^+细胞免疫荧光化学鉴定。结果:①原代培养2周,贴壁细胞呈集落样生长,换液后成熟肝细胞逐渐破碎、消失,小圆形细胞增殖成片,多位于集落中央,呈簇状排列,多数细胞只有1个核,胞核较大,胞浆较少,细胞边界清楚。传至1～2代时仍可见细胞呈集落样生长,生长速度加快,经免疫磁珠分离获得的C-kit^+细胞呈类圆形,胞核较大,胞浆较少,体外培养1周后呈栅栏状、漩涡、片状或不规则样生长。②癌旁组织HE染色后汇管区内可见不典型增生的小胆管,其内部存在小圆形细胞;免疫荧光细胞化学染色后癌旁组织汇管区内增生的小胆管内的小圆形细胞共表达红色荧光AFP和绿色荧光CK19,二者叠加部分呈黄色。③C-kit^+细胞免疫荧光化学后,胞浆内表达AFP红色颗粒与CK19绿色颗粒,二者重叠部分即AFP^+/CK19^+双阳性细胞,呈黄色荧光。结论:人类肝癌旁组织中存在肝脏干细胞,可联合其表面标志物的表达,将C-kit^+/AFP^+/CK19^+作为鉴定分离肝脏干细胞的一种方法。体外分离培养得到的小圆形细胞即卵圆细胞,具备向肝细胞及胆管上皮细胞双向分化的潜能。

关键词：肝肿瘤肝脏干细胞卵圆细胞荧光抗体技术

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

阿托伐他汀钙调节人脐静脉内皮细胞E-选择素和细胞间黏附分子1表达的浓度依赖性

引用

中国临床康复 2005年第46期9卷 56-59页

作者：刘巍立姜一农旅朝霞张英武汉亚洲心脏病医院心内科湖北省武汉市430022 大连医科大学附属第一医院心内科辽宁省大连市116011

目的:观察具有调脂作用的阿托伐他汀钙在不同浓度下对肿瘤坏死因子诱导体外培养的人脐静脉内皮细胞表达E-选择素和细胞间黏附分子1的影响。方法:①实验于2002-05/2003-12在大连医科大学附属第二医院实验室完成。将健康婴儿脐带进行无菌... 详细信息

目的:观察具有调脂作用的阿托伐他汀钙在不同浓度下对肿瘤坏死因子诱导体外培养的人脐静脉内皮细胞表达E-选择素和细胞间黏附分子1的影响。方法:①实验于2002-05/2003-12在大连医科大学附属第二医院实验室完成。将健康婴儿脐带进行无菌处理,用D-hank’s液冲洗脐静脉,1g/l胶原酶消化、离心,置于37℃、体积分数0.05CO2培养箱中培养,选择第2,3代细胞作为实验用。②用肿瘤坏死因子40μg/l、不同浓度(0.005～10μmol/l)的阿托伐他汀钙与体外培养的人脐静脉内皮细胞共同孵育24h后,提取细胞的总RNA,采用半定量反转录聚合酶链反应在mRNA水平进行分析。③用曲线图表示E-选择素和细胞间黏附分子1表达在不同浓度阿托伐他汀钙影响下的变化趋势。结果:①在mRNA水平,阿托伐他汀钙对肿瘤坏死因子诱导的人脐静脉内皮细胞所表达的E-选择素与细胞黏附分子1均具有浓度依赖性。②阿托伐他汀钙对肿瘤坏死因子诱导人脐静脉内皮细胞表达E-选择素mRNA呈促进作用,即阿托伐他汀钙在0～10μmol/l的浓度区间内,随着其浓度由0,0.005,0.01μmol/l渐升至10μmol/l,E-选择素mRNA的表达呈逐渐升高的趋势。③阿托伐他汀钙对肿瘤坏死因子诱导人脐静脉内皮细胞表达细胞间黏附分子1的影响呈双向性,具有浓度差异性。即随着阿托伐他汀钙浓度由0,0.005,0.01μmol/l升至0.05μmol/l,细胞间黏附分子1mRNA表达呈逐渐下降趋势,而在高浓度(0.05～10μmol/l)区间,随着阿托伐他汀钙浓度0.05,0.1μmol/l渐升至10μmol/l,细胞间黏附分子1mRNA的量呈逐渐升高的趋势。结论:在基因水平,阿托伐他汀钙对肿瘤坏死因子诱导血管内皮细胞黏附分子的表达有着确切的调节作用,且存在着浓度依赖性,并在不同的浓度区间,对不同的黏附分子作用不同。

关键词：羟甲基戊二酰基CoA还原酶抑制剂/药理学细胞间粘附分子1 E选择素内皮细胞

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

诱导分化羊骨髓间充质干细胞作为组织工程瓣膜间质种子细胞的可行性

引用

中国组织工程研究与临床康复 2007年第20期11卷 3916-3919页

作者：顾春虎王云雅李亮刘维永卫向阳欧阳辉张金洲解放军第四军医大学西京医院心脏外科陕西省西安市710032 解放军第四军医大学西京医院核医学科陕西省西安市710032

目的:对羊骨髓间充质干细胞加以诱导分化,并与大隐静脉来源的血管间质细胞进行比较,探讨其作为组织工程瓣膜种子细胞的可行性。方法:实验于2006-02/08在西京医院心血管病研究所完成。①家猪10只,屠宰后取主动脉瓣膜,尽量剔去血管外膜,浸... 详细信息

目的:对羊骨髓间充质干细胞加以诱导分化,并与大隐静脉来源的血管间质细胞进行比较,探讨其作为组织工程瓣膜种子细胞的可行性。方法:实验于2006-02/08在西京医院心血管病研究所完成。①家猪10只,屠宰后取主动脉瓣膜,尽量剔去血管外膜,浸入hank’s液中洗涤,制备去细胞主动脉瓣,苏木精-伊红染色比较脱细胞前后的组织特点。②成年杂种绵羊10只,麻醉后分别于股骨大转子穿刺抽取肝素化骨髓10ml,稀释,离心,弃上清及脂肪层,余细胞用lG-DMEM无血清培养基重悬,铺于等体积的Percoll淋巴细胞分离液上,离心收集单个核细胞,消化传代培养。取第二、三代骨髓间充质干细胞,加入lG-DMEM条件培养基进行体外定向诱导分化。对诱导后细胞进行形态观察、免疫组化鉴定并绘制生长曲线。③上述10只绵羊抽取骨髓后,无菌条件下取大隐静脉,剥去静脉外膜,剪成1mm3,贴壁法培养,消化传代。④取第3代骨髓间充质干细胞与血管间质细胞,分别以5×104l-1密度接种于24孔培养板,常规孵育96h。每孔各吸取培养基0.5ml,测定两种细胞上清液中的羟脯氨酸含量。完毕后用胰蛋白酶消化处于对数生长期的细胞,加入冻存液,调整细胞密度为7×109l-1,置于液氮中,1周后复苏,计算两种细胞的存活率。⑤将去细胞猪主动脉瓣修剪成0.5cm×0.5cm×0.1cm大小,骨髓间充质干细胞、血管间质细胞均按105/cm2密度接种,置入37℃、体积分数为0.05的CO2孵箱中。反复种植3次,每次间隔24h,第4天各组均取出2份样本,扫描电镜观察。第7天向其余标本中加入四唑盐,采用酶联免疫检测仪490nm处骨髓间充质干细胞组、血管间质细胞组的吸光度值,比较两种细胞在去细胞主动脉瓣上的生长能力。结果:①新鲜的猪瓣叶中含有大量的成纤维细胞及内皮细胞。去细胞后,主动脉瓣中未见任何细胞及其碎片成分,纤维网架结构保持完整。②传代培养后的骨髓间充质干细胞和血管间质细胞形态相似,呈梭形或多角型,均于24h内贴壁,3～4d铺满瓶底;两种细胞对平滑肌肌动蛋白α、波形蛋白均呈部分阳性表达;且生长曲线相似,倍增时间分别为38h和36h(P>0.05)。③诱导分化后的骨髓间充质干细胞与血管间质细胞上清液羟脯氨酸含量基本相似[(1.52±0.21),(1.43±0.20)mg/l,P>0.05]。冻存复苏后,两种细胞的存活率亦基本相似[(85±3)%,(87±4)%,P>0.05]。④诱导分化后的骨髓间充质干细胞与血管间质细胞均能够种植在去细胞猪主动脉瓣上,两种细胞的吸光度值基本相似(0.50±0.04,0.48±0.03,P>0.05)。结论:诱导分化的骨髓间充质干细胞能够黏附在去细胞猪主动脉瓣膜支架上贴壁生长,与静脉来源的血管间质细胞在存活率、复苏后生长增殖情况、合成胶原功能等方面无明显差别,是合适的组织工程瓣膜间质种子细胞。

关键词：诱导分化种子细胞组织工程间充质干细胞

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：