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Biomineralization of soil with crude soybean urease using different calcium salts

Journal of Rock Mechanics and Geotechnical Engineering 2024年第5期16卷 1788-1798页

作者： Yajie Weng Junjie Zheng hanjiang lai Mingjuan Cui Xingzhi Ding School of Civil and Hydraulic Engineering Huazhong University of Science and TechnologyWuhan430074China Zijin School of Geology and Mining Fuzhou UniversityFuzhou350108China College of Civil Engineering Fuzhou UniversityFuzhou350116China

Calcium salt is an important contributing factor for calcium-based *** study the effect of calcium salt on soil biomineralization using crude soybean urease,the calcium salts,including the calcium chloride (CaCl_(2)),calcium acetate ((CH_(3)COO)_(2)Ca) and calcium nitrate (Ca(NO_(3))_(2)),were used to prepare the biotreatment solution to carry out the biomineralization tests in this *** series of biomineralization tests in solution and sand column,respectively,were *** electron microscopy (SEM) and X-ray diffraction (XRD) were performed to determine the microscopic characteristics of the precipitated calcium carbonate (CaCO_(3)) *** experimental results indicate that the biomineralization effect is the best for the CaCl2 case,followed by (CH_(3)COO)_(2)Ca,and worst for Ca(NO_(3))_(2) under the test conditions of this study (i.e.1 mol/L of calcium salt-urea).The mechanism for the effect of the calcium salt on the biomineralization of crude soybean urease mainly involves: (1) inhibition of urease activity,and (2) influence on the crystal size and morphology of CaCO_(3).Besides Ca^(2+) ,the anions in solution can inhibit the activity of crude soybean urease,and NO_(3)− has a stronger inhibitory effect on the urease activity compared with both CH_(3)COO^(−) and Cl^(−) .The co-inhibition of Ca^(2+) and NO_(3)− on the activity of urease is the key reason for the worst biomineralization of the Ca(NO_(3))_(2) case in this *** difference in biomineralization between the CaCl_(2) and (CH_(3)COO)_(2) Ca cases is strongly correlated with the crystal morphology of the precipitated CaCO_(3).

关键词： Biomineralization Crude soybean urease Calcium salt Influence mechanism

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Recent development in biogeotechnology and its engineering applications

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Frontiers of Structural and Civil Engineering 2021年第5期15卷 1073-1096页

作者： hanjiang lai Shifan WU Mingjuan CUI Jian CHU Zijin School of Geology and Mining Fuzhou UniversityFuzhou 350108China School of Civil and Environmental Engineering Nanyang Technological UniversitySingapore 639798Singapore College of Civil Engineering Fuzhou UniversityFuzhou 350108China

Microbial geotechnology or biogeotechnology is a new branch of geotechnical *** involves the use of microbiology for traditional geotechnical *** new innovative soil improvement methods have been developed in recent years based on this approach.A proper understanding of the various approaches and the performances of different methods can help researchers and engineers to develop the most appropriate geotechnical *** present,most of the methods can be categorized into three major types,biocementation,bioclogging,and biogas *** and differences of different approaches and their potential applications are *** affecting the different processes are also *** of up-scaled model tests and pilot trials are presented to show the emerging *** challenges and problems of biogeotechnology are also discussed.

关键词： biogeotechnology biocementation bioclogging biogas strength enhancement liquefaction mitigation seepage control

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Zero-Shot Medical Image Retrieval for Emerging Infectious Diseases Based on Meta-Transfer Learning--Worldwide,2020

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China CDC weekly 2020年第52期2卷 1004-1008页

作者： Yuying Zhao hanjiang lai Jian Yin Yewu Zhang Shigui Yang Zhongwei Jia Jiaqi Ma School of Computer Science and Engineering Sun Yat-Sen UniversityGuangzhouGuangdongChina Center for Public Health Surveillance and Information Service Chinese Center for Disease Control and PreventionBeijingChina State Key Laboratory for Diagnosis and Treatment of Infectious Diseases Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesThe First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhouZhejiangChina School of Public Health Peking UniversityBeijingChina

Introduction:Due to the increasing number of medical images,image retrieval has become an important technique for medical image *** many content-based image retrieval methods have been proposed,the retrieval of images in datasets related to emerging/new infectious diseases still remain a challenge-mostly due to the lack of historical *** a result,the current retrieval models have limited functionality in helping doctors make accurate diagnoses of new ***:In this paper,we propose a zero-shot retrieval model based on meta-learning and ensemble learning,which can obtain a model with stronger generalizability without using any relevant training data,and thus performs well on new types of test ***:The experimental results showed that the proposed method is 3% to 5% higher than the traditional method,which means that our model can retrieve relevant medical images more accurately for newly emerging data types and provide doctors with more effective ***:When a new infectious disease occurs,doctors can use the proposed zero-shot retrieval model to retrieve all relevant cases,quickly find the common problems of patients,find the locations of the new infections,and determine its infectivity as soon as *** proposed method is a new computeraided decision support technology for emerging infectious diseases.

关键词： Image image retrieval

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MICP反应引入NBPT对碳酸钙沉积的影响

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华中科技大学学报（自然科学版） 2022年第2期50卷 1-6页

作者：徐望清郑俊杰崔明娟赖汉江华中科技大学土木与水利工程学院湖北武汉430074 福州大学土木工程学院福建福州350108

在进行前期探究试验并取得一定成果的基础上,进一步将正丁基硫代磷酰三胺(NBPT)应用于微生物诱导碳酸钙沉积(MICP)固化砂柱试验,结合无侧限抗压强度试验、生成物含量检测、扫描电镜试验、能谱分析及X射线衍射试验等监测手段,对反应生成... 详细信息

在进行前期探究试验并取得一定成果的基础上,进一步将正丁基硫代磷酰三胺(NBPT)应用于微生物诱导碳酸钙沉积(MICP)固化砂柱试验,结合无侧限抗压强度试验、生成物含量检测、扫描电镜试验、能谱分析及X射线衍射试验等监测手段,对反应生成物进行深入研究,分析NBPT对MICP反应生成碳酸钙的影响作用效果.结果表明:NBPT参与MICP反应对诱导产生的碳酸钙晶体形貌及大小具有调控作用.NBPT摩尔分数不高于0.1%时碳酸钙晶体主要为方解石,摩尔分数在0.1%~1%之间时碳酸钙晶体为方解石与球霰石,而摩尔分数高于1%时碳酸钙晶体几乎全部为球霰石.此外,NBPT还可与碳酸钙晶体形成共聚体从而进一步提高MICP反应的胶结效果.

关键词：微生物固化脲酶抑制剂碳酸钙晶体作用机理微观形貌

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Predicting the Incident Cases of Emerging Infectious Disease Using a Bayesian Probability Model--China,February 2020

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China CDC weekly 2020年第52期2卷 999-1003页

作者： Yewu Zhang Xiaofeng Wang Yanfei Li Siyu Wu Ming Wan Xuemei Su Shigui Yang hanjiang lai Zhongwei Jia Jiaqi Ma Center for Public Health Surveillance and Information Service Chinese Center for Disease Control and PreventionBeijingChina School of Public Health Peking UniversityBeijingChina State Key Laboratory for Diagnosis and Treatment of Infectious Diseases Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesThe First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhouZhejiangChina Sun Yat-sen University GuangzhouGuangdongChina

What is already known about this topic?The exact number of incident cases of emerging infectious diseases on a daily basis is of great importance to the disease control and prevention,but it is not directly available from the current surveillance system in *** is added by this report?In this study,a Bayesian statistical method was proposed to estimate the posterior parameters of the gamma probability distribution of the lag time between the onset date and the reporting time based on the surveillance *** then the posterior parameters and corresponding cumulative gamma probability distribution were used to predict the actual number of new incident cases and the number of unreported cases per *** proposed method was used for predicting COVID-19 incident cases from February 5 to February 26,*** final results show that Bayesian probability model predictions based on data reported by February 28,2020 are very close to those actually reported a month *** are the implications for public health practice?This research provides a Bayesian statistical approach for early estimation of the actual number of cases of incidence based on surveillance data,which is of great value in the prevention and control practice of epidemics.

关键词： prevention Probability incident

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Simultaneous detection and characterization of toxigenic Clostridium difficile directly from clinical stool specimens

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Frontiers of Medicine 2018年第2期12卷 196-205页

作者： hanjiang lai Chen Huang Jian Cai Julian Ye Jun She Yi Zheng Liqian Wang Yelin Wei Weijia Fang Xianjun Wang Yi-Wei Tang Yun Luo Dazhi Jin The First People's Hospital of Xiaoshan District Hangzhou 311021 China Department of Microbiology Zhejiang Provincial Center for Disease Control and PreventionHangzhou 310051 China Department of Disease Control and Prevention Zhejiang Provincial Center for Disease Control and PreventionHangzhou 310051 China Biotherapy Center for Medical Oncology the First Affiliated Hospital Zhejiang University Hangzhou 310003 China Department of Laboratory Medicine HangzhouFirst People's Hospital Hangzhou 310006 China Department of Laboratory Medicine Memorial Sloan Kettering Cancer Center Department of Pathology and Laboratory Medicine Weill Medical College of Cornell University New York NY 10065 USA

We employed a multiplex polymerase chain reaction （PCR） coupled with capillary electrophoresis （mPCR-CE） targeting six Clostridium difficile genes, including tPi, tcdA, tcdB, cdtA, cdtB, and a deletion in tcdC for simultaneous detection and characterization of toxigenic C. difficile directly from fecal specimens. The mPCR-CE had a limit of detection of 10 colony-forming units per reaction with no cross-reactions with other related bacterial genes. Clinical validation was performed on 354 consecutively collected stool specimens from patients with suspected C. difficile infection and 45 isolates. The results were compared with a reference standard combined with BD MAX Cdiff, real-time cell analysis assay （RTCA）, and mPCR-CE. The toxigenic C. difficile species were detected in 36 isolates and 45 stool specimens by the mPCR-CE, which provided a positive rate of 20.3% （81/399）. The mPCR-CE had a specificity of 97,2% and a sensitivity of 96.0%, which was higher than RTCA （χ^2 = 5.67, P = 0.017） but lower than BD MAX Cdiff （P = 0.245）. Among the 45 strains, 44 （97.8%） were determined as non- ribotype 027 by the mPCR-CE, which was fully agreed with PCR ribotyping. Even though ribotypes 017 （n = 8, 17.8%）, 001 （n = 6, 13.3%）, and 012 （n = 7, 15.6%） were predominant in this region, ribotype 027 was an important genotype monitored routinely. The mPCR-CE provided an alternative diagnosis tool for the simultaneous detection of toxigenic C. difficile in stool and potentially differentiated between RT027 and non-RT027.

关键词： Clostridium difficile multiplex PCR capillary electrophoresis detection characterization

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基于脲酶诱导碳酸钙沉积固化土体的研究进展

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高校地质学报 2021年第6期27卷 769-774页

作者：赖汉江崔明娟福州大学紫金地质与矿业学院福州350108 福州大学土木工程学院福州350116

诱导碳酸钙沉积的土体固化是近年来岩土工程领域新兴起的新型环保地基处理技术,该技术利用产脲酶菌的微生物诱导碳酸钙沉积(Microbially Induced Calcite Precipitation,即MICP技术)或基于脲酶的酶诱导碳酸钙沉积(Enzyme Induced Carbon... 详细信息

诱导碳酸钙沉积的土体固化是近年来岩土工程领域新兴起的新型环保地基处理技术,该技术利用产脲酶菌的微生物诱导碳酸钙沉积(Microbially Induced Calcite Precipitation,即MICP技术)或基于脲酶的酶诱导碳酸钙沉积(Enzyme Induced Carbonate Precipitation,即EICP技术),将松散的土体颗粒胶结成为整体,达到提高土体抗剪强度的目的。与MICP技术相比,EICP技术不存在生物安全风险,无需考虑是否有氧,且可适用于更小粒径土体的处理,因此具备广阔的实际工程应用前景。文章从脲酶类型与来源、EICP固化土体处理方法及EICP固化土体强度增长等方面,对近20年基于脲酶诱导碳酸钙沉积固化土体的研究进行了回顾与总结。

关键词：地基处理诱导碳酸钙沉积产脲酶菌脲酶尿素水解

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新加坡南洋理工大学实验室安全管理及启示

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实验技术与管理 2022年第5期39卷 250-254页

作者：赖汉江郑俊杰陈志波吴仕帆崔明娟福州大学紫金地质与矿业学院福建福州350116 华中科技大学土木与水利工程学院湖北武汉430074 南洋理工大学土木与环境工程学院新加坡639798 福州大学土木工程学院福建福州350116

高校实验室安全直接关系到师生的生命财产安全及教学科研工作的顺利开展,因此安全管理是高校实验室建设的重点工作之一。为提升高校实验室安全管理水平,该文从实验室的准入制度、安全手册、硬件设施保障以及监管制度等方面,对新加坡南... 详细信息

高校实验室安全直接关系到师生的生命财产安全及教学科研工作的顺利开展,因此安全管理是高校实验室建设的重点工作之一。为提升高校实验室安全管理水平,该文从实验室的准入制度、安全手册、硬件设施保障以及监管制度等方面,对新加坡南洋理工大学实验室安全管理体系进行了梳理与评述。在此基础上,从细化规章制度、完善硬件设施、加强管理队伍建设等三个方面,对我国高校实验室的安全管理提出了一些参考建议。

关键词：实验室安全安全管理准入制度监管制度

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格栅加筋砂土拉拔试验界面特性的离散元模拟

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华中科技大学学报（自然科学版） 2014年第8期42卷 104-108页

作者：郑俊杰周燕君赖汉江孙玲华中科技大学土木工程与力学学院湖北武汉430074

采用二维颗粒流程序PFC2D建立格栅加筋砂土的格栅拉拔试验数值分析模型,分析了格栅拉拔过程中位移场、接触力、孔隙率、配位数等参数的变化规律.根据剪切带厚度将试样划分成四个区域,通过开发细观组构统计程序记录格栅拉拔过程中各区域... 详细信息

采用二维颗粒流程序PFC2D建立格栅加筋砂土的格栅拉拔试验数值分析模型,分析了格栅拉拔过程中位移场、接触力、孔隙率、配位数等参数的变化规律.根据剪切带厚度将试样划分成四个区域,通过开发细观组构统计程序记录格栅拉拔过程中各区域砂土细观组构演化,探讨砂土颗粒的接触法向、接触力分量的各向异性演化规律及其与试样宏观抗剪强度之间的关系.研究结果表明:拉拔试验过程中,剪切带内平均法向接触力增大,切向接触力减小.剪切带内砂土抗剪强度受控于法向接触力及其各向异性的变化,拉拔过程中,砂土颗粒间法向接触力各向异性主方向的变化与大主应力的方向相一致.

关键词：格栅加筋砂土拉拔试验数值模拟离散元组构各向异性

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萧山区基层医院围产期孕妇B族链球菌筛查及流行病学研究

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中国性科学 2022年第2期31卷 86-89页

作者：袁志平来汉江沈丽萍浙江省杭州市萧山区第二人民医院检验科杭州311241 浙江省杭州市萧山区第一人民医院微生物科杭州311200

目的探究萧山区基层医院围产期孕妇B族链球菌(GBS)筛查的意义及流行病学特点。方法回顾性选取2020年5月至2021年6月于杭州市萧山区基层医院产科门诊就诊的1225例围产期孕妇作为研究对象,检测其GBS感染情况,对GBS阳性菌株进行耐药性检测... 详细信息

目的探究萧山区基层医院围产期孕妇B族链球菌(GBS)筛查的意义及流行病学特点。方法回顾性选取2020年5月至2021年6月于杭州市萧山区基层医院产科门诊就诊的1225例围产期孕妇作为研究对象,检测其GBS感染情况,对GBS阳性菌株进行耐药性检测,并分析GBS感染对妊娠结局的影响。结果1225例孕妇中,GBS阳性孕妇98例(8.00%);GBS阳性孕妇早产､宫内感染､胎膜早破､产褥感染发生率明显高于GBS阴性孕妇(P<0.05);GBS阳性孕妇分娩的新生儿肺炎､窒息､宫内窘迫的发生率明显高于GBS阴性孕妇分娩的新生儿(P<0.05)。常用抗菌药物中,GBS菌株对利奈唑胺､万古霉素的敏感率高达100.00%,对氨苄西林､青霉素､头孢曲松､头孢唑林的敏感率均在90.00%以上,而对四环素的耐药率仅为60.21%。结论孕妇GBS感染可造成早产､宫内感染､胎膜早破､产褥感染,以及新生儿肺炎､窒息､宫内窘迫等不良妊娠结局,GBS菌株对利奈唑胺､万古霉素､氨苄西林､青霉素､头孢曲松等抗菌药物均有较高敏感性。

关键词：萧山区基层医院围产期孕妇 B族链球菌

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