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REMARKS ON h-TRANSFORM AND DRIFT

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Chinese Annals of Mathematics,Series B 1998年第4期19卷 473-478页

作者： YING JIANGANG (Department of Mathematics, Zhejiang University, hangzhou 310027, China.) Department of Mathematics Zhejiang University Hangzhou 310027 China

The author studies the h-transforms of symmetric Markov processes and corresponding Dirichlet spaces, and also discusses the drift transformation of Fukushima and Takeda’s type[2] and improves their result by a diffe... 详细信息

关键词： Right processes Revuz measures h-transform

Isolation and Partial Characterization of a Novel Pollen-specific cDNA with Multiple Polyadenylation Sites from Wheat

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Acta Biochimica et Biophysica Sinica 2004年第7期36卷 467-476页

作者： Yong-Feng JIN* and Teng-Fei BIANInstitute of Biochemistry, Zhejiang University, hangzhou 310029, China Institute of Biochemistry Zhejiang UniversityHangzhou 310029 China

A novel pollen-specific full-length cDNA clone PSG076 was isolated using suppression subtractive hybridization and 5’/3’ RACE techniques. PSG076 was shown to exhibit multi-site polyadenylation by sequencing the 3’ ends of the cDNAs. At least six transcripts with different length were produced from the single gene based on different poly(A) tail attachment sites. However, polyadenylation consensus sequence AAUAAA was not seen at the 3’-untranslated sequence. PSG076 contained a 299 bp 5’ untranslated region and an open reading frame of 663 bp encoding a 221 amino acid peptide with pI of 4.31. A blast search revealed that this sequence did not show a significant similarity to any genes deposited in the public database. Southern blot indicated that PSG076 was a single copy gene. Northern blot and RT-PCR analysis indicated that PSG076 transcripts showed specific expression in mature pollen, and weak or undetectable signals in uninucleate microspore, immature seed, stem, young leave, root and ovary. Further analysis of the expression pattern in gametophyte showed that PSG076 transcripts were undetectable in uninucleate, binucleate microspore and pollen at early stage, and were first detectable and increased rapidly at middle and late stages of pollen development with the maximum level in mature pollen and also expressed in germinating pollen in vivo, suggesting that PSG076might play a role in pollen germination and pollen tube growthin addition to its function in maturation. The evidences gathered in this work indicated that the six different transcripts from the single gene were differentially expressed during pollen development.

关键词： wheat (Triticum aestivum L.) pollen-specific cDNA suppression subtractive hybridization multiple-site polyadenylation differential expression

Interpolating triangular meshes by Loop subdivision scheme

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Science China(Information Sciences) 2010年第9期53卷 1765-1773页

作者： DENG ChongYang1* & WANG GuoZhao2 1Institute of Applied Mathematics and Engineering Computations,hangzhou Dianzi University,hangzhou 310018,China 2Department of Mathematics,Zhejiang University,hangzhou 310027,China Institute of Applied Mathematics and Engineering Computations Hangzhou Dianzi University Hangzhou China Department of Mathematics Zhejiang University Hangzhou China

Using the limit point formula of the Loop subdivision scheme,we propose a very simple and efficient method for constructing interpolation surface of triangular meshes by Loop subdivision *** excellent properties of the method are:(1) Locality:the perturbation of a given vertex only influences the surface shape near this vertex.(2) Efficiency:the locations of new points can be computed with explicit formulae.(3) Easiness in implementation:only the geometric rule of the first step should be modified.(4) Freedom:for each edge,there is one degree of freedom to adjust the shape of the interpolation surface.(5) Easiness in generalization:it is easy to generalize our method to other approximation subdivision schemes with explicit formulae to compute limit point.

关键词： computer aided geometric design subdivision surface approximating subdivision scheme Loop surface surface interpolating

ON COMPLETE CONVERGENCE OF NONPARAMETRIC REGRESSION M-QUANTILES

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Systems Science and Mathematical Sciences 1992年第3期5卷 227-232页

作者： CAI Zongwu (Department of Mathematics,hangzhou University,hangzhou 310028,China) 杭州大学浙江杭州

Consider the nonparametric regression model Y_i=m(x_i)+ε_i,i=1,…,n,where m(?)is an unknown function,and the design points x_i are knownand *** robust nonparametric estimators were introduced by H(?)rdleand Gasser in *** estimators can be viewed as regression *** then establish complete convergence for such quantiles under only the finitemoment condition.

关键词： Nonparametric kernel type estimator M-smoother quantile complete convergence

ON SIMULTANEOUS APPROXIMATION TO A DIFFERENTIABLE FUNCTION AND ITS DERIVATIVE BY INVERSE PAL-TYPE INTERPOLATION POLYNOMIALS

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Analysis in Theory and Applications 1995年第4期11卷 15-23页

作者： Bao Yongguang (hangzhou University, China) Res. Ins. of Decision-making and Optimization Hangzhou University Hangzhou PRC

Let ξn-1 and n is even thenholds uniformly for all x ∈ [- 1,1], where h(x) = 1 +

关键词： Math In ON SIMULTANEOUS APPROXIMATION TO A DIFFERENTIABLE FUNCTION AND ITS DERIVATIVE BY INVERSE PAL-TYPE INTERPOLATION POLYNOMIALS PAL ITS

Highly Sensitive Fluorescent-labeled Probes and Glass Slide Hybridization for the Detection of Plant RNA Viruses and a Viroid

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Acta Biochimica et Biophysica Sinica 2007年第5期39卷 326-334页

作者： Zhiyou DU~(1,2), Bo JIN~3, Wenhong LIU~4, Liang CHEN~1, and Jishuang CHEN~(2,1*) 1 College of Life Sciences, Zhejiang University, hangzhou 310029, China 2 Institute of Bioengineering, Zhejiang Sci-Tech University, hangzhou 310018, China 3 College of Life Sciences, Zhejiang Chinese Medical University, hangzhou 310053, China 4 College of Bioengineering, Zhejiang Chinese Medical University, hangzhou 310053, China College of Life Sciences Zhejiang UniversityHangzhou 310029 China Institute of Bioengineering Zhejiang Sci-Tech UniversityHangzhou 310018 China College of Life Sciences Zhejiang Chinese Medical UniversityHangzhou 310053 China College of Bioengineering Zhejiang Chinese Medical UniversityHangzhou 310053 China

In this study, a modified method of the conventional RNA dot-blot hybridization was established, by replacing32p labels with CY5 labels and replacing nylon membranes with positive-charged glass slides, for detecting plant RNA viruses and a viroid. The modified RNA dot-blot hybridization method was named glass slide hybridization. The optimum efficiency of RNA binding onto the surfaces of activated glass slide was achieved using aminosilane-coated glass slide as a solid matrix and 5×saline sodium citrate （SSC） as a spotting solution. Using a CY5-labeled DNA probe prepared through PCR amplification, the optimized glass slide hybridization could detect as little as 1.71 pg of tobacco mosaic virus （TMV） RNA. The sensitivity of the modified method was four times that of dot-blot hybridization on nylon membrane with a32P-labeled probe. The absence of false positive within the genus Potyvirus [potato virus A, potato virus Y （PVY） and zucchini yellow mosaic virus] showed that this method was highly specific. Furthermore, potato spindle tuber viroid （PSTVd） was also detected specifically. A test of 40 field potato samples showed that this method was equivalent to the conventional dot-blot hybridization for detecting PVY and PSTVd. To our knowledge, this is the first report of using dot-blot hybridization on glass slides with fluorescent-labeled probes for detecting plant RNA viruses and a viroid.

关键词： glass slide hybridization fluorescent labeling dot-blot hybridization virus viroid

ABOUT THE FOURIER TRANSFORMS OF NON-SMOOTH MEASURES ON HYPERSPHERES

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Analysis in Theory and Applications 1995年第1期11卷 10-15页

作者： Sun Limin(hangzhou University, China) Department of Mathematics Hangzhou University Hangzhou Zhejiang P.R.C.

If dμ is the Fourier transform of a smooth measure,dμ on the hypersphere Sn-1(n≥2)the there exists a constant C dependent only on n such that |dμ(y) |≤C(1+ |y |)-(n-1) /2 for all y∈Rn. In this paper, we show that the above statement is false for non-smooth measures. And we present the corresponding estimations far the Fourier transforms of certain non-smooth measures on Sn-1.

关键词： ABOUT THE FOURIER TRANSFORMS OF NON-SMOOTH MEASURES ON HYPERSPHERES

In-situ FTIR studies of dimethyl ether adsorption on H-ZSM-5 zeolites

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Chinese Journal of Chemistry 1996年第6期14卷 497-505页

作者： JIANG, Xuan-ZhenDepartment of Chemistry, Zhejiang University, hangzhou, Zhejiang 310027, China Department of Chemistry Zhejiang University Hangzhou Zhejiang 310027 China

In situ infrared spectra of dimethyl ether adsorbed on two H-ZSM-5 zeolites are reported which are different in both lattice and extraframework aluminium contents. A number of dissociatively adsorbed dimethyl ether species associated respectively with the Bronsted acid sites, the external silanol groups and extraframework aluminium species at different temperatures are identified. In particular, dimethyl ether reacts with non acidic hydroxyl species associated with extraframework aluminium to form the third methoxy species. The distributions of hydrocarbons desorbed from a single pulse of dimethyl ether (1.0 mL) onto these zeolite samples are presented. Propene is the major product, and the yield of hydrocarbons correlates with the concentration of Bronsted acid bound methoxy groups.

关键词： In situ FTIR dimethyl ether adsorption extraframework aluminium bound methoxy species hydrocarbon products

Interaction between a Nanovirus-like Component and the Tobacco Curly Shoot Virus/Satellite Complex

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Acta Biochimica et Biophysica Sinica 2005年第1期37卷 25-31页

作者： Pei-Jun WU Xue-Ping ZHOU~* Institute of Biotechnology Zhejiang University,hangzhou 310029,China Institute of Biotechnology Zhejiang UniversityHangzhou 310029 China

The biological role of DNA1,a nanovirus-like component shown to be associated with thebegomovirus/satellite complex,has not yet been ***,we demonstrated that DNAl of Tobaccocurly shoot virus isolate Y35(TbCSV-Y35)attenuated leaf-curling symptoms induced by TbCSV-Y35 orTbCSV-Y35 plus Y35 DNAβ in the early stage of symptom development and induced leaf cluster at a laterstage of symptom development in Nicotiana benthamiana *** leaf disc assay demonstrated thatTbCSV-Y35 DNA 1 replicated *** blot analysis revealed that TbCSV-Y35 DNA 1 re-duced viral DNA *** DNA accumulation was not reduced when plants were co-inoculatedwith TbCSV-Y35 DNAβ,but the TbCSV-Y35 DNAβ level was dramatically reduced in the presence ofTbCSV-Y35 DNA *** determine whether the interaction between TbCSV/satellite complex and DNA 1 hadisolate specificity,DNAI of TbCSV isolate Y132 was cloned and *** was found to have 75%nucleotide sequence identity with TbCSV-Y35 DNA *** tests showed that TbCSV-Y 132 DNA 1 hadno effect on the symptoms induced by TbCSV-Y35 or TbCSV-Y35 and Y35 DNAβ in ***,although Y 132 DNA1 could replicate in these plants.

关键词： Tobacco curly shoot virus DNA1 DNAβ symptom replication interaction