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Protective effect of nitric oxide on hepatopulmonary syndrome from ischemia-reperfusion injury

World Journal of Gastroenterology 2012年第25期18卷 3310-3316页

作者： Tong-Jin Diao, Xin Chen Li-Hua Deng, han-xiang cheni Yan Liang Xiao-Dong Zhao Qing-Hua Wang, Wei-Sheng Yuan Bai-Chun Gao Yong Ye Department of Hepatobiliary Surgery and LiverTransplant Center Jinan Military Region The Chinese PLA401 st Hospital Qingdao 266071 Shandong Province China Department of Emergency The People's Hospital ofChengyang District Qingdao 266109 Shandong Province ChinaI_i-Hua Deng Central Injection Room Qingdao Municipal Hos-pital Qingdao 266011 Shandong Province China Central Injection Room Qingdao Municipal Hos-pital Qingdao 266011 Shandong Province China Institute of-Pathogenic Biology School ofMedicine Shandong University Jinan 250012 Shandong Prov-ince China College of Marine Life Sciences Ocean Universityof China Qingdao 266003 Shandong Province China Department of General Surgery Jinan MilitaryRegion the Chinese PLA 401st Hospital Qingdao 266071 Shan-dong Province China

AIM： To evaluate immunological protection of nitric ox- ide （NO） in hepatopulmonary syndrome and probable mechanisms of ischemia-reperfusion （IR） injury in rat liver transplantation, METHODS： Sixty-six healthy male Wistar rats were randomly divided into three groups （11 donor/recipi-ent pairs）. In group 11, organ preservation solution was lactated Ringer＇s solution with heparin 10 000/μL at 4℃. In groups I and 111, the pLeservation solution added, respectively, L-arginine or Ng-L-arginine methyl ester （L-NAME）（1 mmol/L） based on group 11, and recipients were injected with L-arginine or L-NAME （50 mg/kg） in the anhepatic phase. Grafted livers in each group were stored for 6 h and implanted into recipi- ents. Five rats were used for observation of postopera- tive survival in each group. The other six rats in each group were used to obtain tissue samples, and execut- ed at 3 h and 24 h after transplantation. The levels of alanine aminotransferase （ALT）, tumor necrosis factor （TNF）-a and NO metabolites （NOx） were detected, and expression of NO synthase, TNF-α and intercellular adhesion molecule 1 （ICAM-1） was examined by tri- phosphopyridine nucleotide diaphorase histochemical and immunohistochemical staining. RESULTS： By supplementing L-arginine to strengthen the NO pathway, a high survival rate was achieved and hepatic function was improved. One-week sur- viral rate of grafted liver recipients in group I was significantly increased （28.8 4±36.6 d ys 4 4±1.7 d, P 〈 0.01） as compared with groups 11 and Ill. Serum levels of ALT in group ] were 2-7 times less than those in groups 11 and 11I （P 〈 0.01）. The cyclic guanosine monophosphate （cGMP） levels in liver tissue and NOx in group I were 3-4 times higher than those of group 11 after 3 h and 24 h reperfusion, while in group ]]], they were significantly reduced as compared with those in group ]1 （P 〈 0.01）. The levels of TNF-（z in group I were significantly lower than in group Ⅱ after 3 h and 24 h reperfusion （P 〈 0.01）,

关键词： Nitric oxide Nitric oxide synthase Immu-noregulatory Hepatopulmonary syndrome Ischemia-reperfusion injury Orthotopic liver transplantation

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Factors Influencing the Accuracy of Sonographyestimated Fetal Weight:A Retrospective Cohort Study in the Chinese Population

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Reproductive and Developmental Medicine 2023年第4期7卷 203-209页

作者： Fang-Can Sun Hai-Hong Wang Jing-Jing Zhang Xin-Xian Gu Ting Hu Ming-Yue Wang Yin-Di Zhu You-Guo cheni Bing han Department of Obstetrics and Gynecology theFirst Affiliated Hospital of Soochow UniversitySuzhou 215006China Department of Obstetrics and Gynecology the Afiliated Hospital of Xuzhou Medical UniversityXuzhou 221006China Department of Ultrasound Dushu Lake Hospital Affiliated to Soochow UniversitySuzhou 215000China Department of Obstetrics and Gynecology Ultrasound the First Affliated Hospital of Soochow UniversitySuzhou 215006China.

Objective:To evaluate the accuracy of sonography-estimated fetal weight(EFW)and to determine the potential factors influencing EFW accuracy in the Chinese ***:Eleven widely used formulae were chosen to evaluate the accuracy of EFW in 7016 cases in a retrospective cohort *** potential factors(prenatal body mass index[BMI],gestational age[GA],birth weight[BW],fetal sex,fetal presentation,amniotic fluid volume[AFV],seniority of sonographers,the time interval between ultrasonic measurement and delivery,and diabetes mellitus)were analyzed by binary logistic regression to determine whether they influenced ***:All 11 models had high correlation coefficients between EFW and BW(r=0.819–0.843).Model 3 was the most accurate formula for the Chinese *** to fetuses with EFW of 2500–3999g,the accuracy of EFW decreased by 69.10%(Phan 2500g,and decreased by 54.10%(Phan *** accuracy of EFW in female fetuses decreased by 12.70%compared with male fetuses(P=0.021).Compared to the EFW calculated 1-3 days before delivery,the accuracy of the EFW performed 4–7 days before delivery showed a significant decrease(P=0.014).The accuracy of EFW was influenced by the individual measurement accuracy of the *** BMI,DM,GA,fetal presentation,and AFV did not influence the accuracy of EFW after ***:Model 3 was the most accurate model for the Chinese *** BW,fetal sex,time interval,and technique of the sonographers had different influences on the accuracy of EFW.

关键词： Accuracy Estimated fetal weight Ultrasonography Fetal sex Birth weight

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蓬子菜中香叶木苷抗血栓作用研究

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哈尔滨商业大学学报（自然科学版） 2016年第4期32卷 390-394页

作者：隋文霞董坤万晓晨寇韩旭马英丽黑龙江中医药大学药学院哈尔滨150040 黑龙江省中医药科学院哈尔滨150036

为研究蓬子菜活性成分香叶木苷改善血液流变学异常和抗静脉血栓作用,通过结扎大鼠下腔静脉造成下肢静脉血栓模型,通过检测血液流变学指标,以及观察下腔静脉在透射电镜下的组织学变化,考察香叶木苷对血栓大鼠血液流变学和内皮功能的影响... 详细信息

为研究蓬子菜活性成分香叶木苷改善血液流变学异常和抗静脉血栓作用,通过结扎大鼠下腔静脉造成下肢静脉血栓模型,通过检测血液流变学指标,以及观察下腔静脉在透射电镜下的组织学变化,考察香叶木苷对血栓大鼠血液流变学和内皮功能的影响.结果表明,与模型组相比,蓬子菜中香叶木苷能明显改善血栓大鼠血液流变学指标,并可有效改善下腔静脉内皮细胞功能.蓬子菜活性成分香叶木苷具有改善血液流变学异常和抗静脉血栓形成的作用.

关键词：蓬子菜香叶木苷静脉血栓血液流变学

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我国杂鳞库蚊亚组的初步研究

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昆虫学报 1980年第4期23卷 434-440页

作者：陈汉彬贵阳医学院生物学教研室

Sirivanakarn(1975)将海滨库蚊组(Culex sitiens group)杂鳞库蚊亚组(C,vishnui subgroup)分为三个复组,即*** complex、*** complex和*** complex等。本亚组在东洋界已知有8种,其中包括一些成虫形态相似,但幼虫区别明显的近缘种。其共同特征是:阳茎侧板内叶(腹支)密生小刺;中叶分4—5个指状突;外叶的背、腹齿约等大(图1)。载肛突基内侧有侧楔(lateral peg)。喙和跗有淡色坏。由于杂鳞库蚊亚组与医学关系密切而又难以鉴别,近年来国外对其分类已进行了一些研究。

关键词：库蚊环带广州模式标本初步研究库蚊属广东亚组地模标本

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