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Molecular Cloning of sodB Gene from Vibrio alginolyticus HY9901 and Its Bioinformatics Analysis

Asian Agricultural Research 2024年第7期16卷 42-47页

作者： Shuai YANG Yingying JIANG haiyun feng Weijie ZHANG Na WANG Xiaonan LU Huanying PANG Fisheries College Guangdong Ocean UniversityZhanjiang 524025China Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture&Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes Zhanjiang 524025China Chinese Academy of Inspection and Quarantine Beijing100176China Department of Food Science and Agricultural Chemistry Faculty of Agricultural and Environmental SciencesMcGill UniversitySainte-Anne-de-Bellevue H9X 3V9Canada

Vibrio alginolyticus is a zoonotic bacterium.A pair of specific primers was designed using the sodB gene sequence of Vibrio alginolyticus HY9901 in order to amplify the full length of the gene by *** results indicated that the total length of the sodB gene was 585 bp and that it could encode 194 amino *** predicted amino acid sequence derivation indicated that the molecular weight of the protein was approximately 21.56 kDa,with an isoelectric point of *** prediction of the N-terminal signal peptide structure of the protein,no significant signal peptide cleavage site was observed,indicating that the protein lacked both a signal peptide and a transmembrane *** amino acid sequence contained an N-glycosylation site,a casein kinase II phosphorylation site,a microsomal C-terminal target signal site,and a manganese and iron superoxide dismutase signal *** probability of intracytoplasmic localization of the SodB protein was 56.5%,which was analyzed according to the subcellular localization of the *** amino acid sequence of the sodB gene of *** exhibited 98%-100%homology to other Vibrio species,clustering into the same subfamily with ***,indicating a relatively close relationship between *** the prediction of protein structure,the proportions ofα-helix,random coil,β-sheet,and extended strand were 48.45%,30.41%,5.67%,and 15.46%,*** similarity to template 1dt0.1.A reached 71.58%.A PTM site analysis revealed the presence of phosphorylation,glycosylation,ubiquitination,sumoylation,acetylation,and methylation modification sites,as well as the absence of lactylation modification sites.

关键词： Vibrio alginolyticus Gene cloning sodB gene Bioinformatics analysis

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Gene Cloning and Bioinformatics Analysis of phoR Gene from Vibrio alginolyticus HY9901

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Asian Agricultural Research 2024年第6期16卷 36-40页

作者： Xiangyu LIU Peng ZHOU haiyun feng Weijie ZHANG Huanying PANG Na WANG Xiaonan LU Fisheries College Guangdong Ocean UniversityZhanjiang 524025China Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture&Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes Zhanjiang 524025China Chinese Academy of Inspection and Quarantine Beijing100176China Department of Food Science and Agricultural Chemistry Faculty of Agricultural and Environmental SciencesMcGill UniversitySainte-Anne-de-Bellevue H9X 3V9Canada

PhoR is a histidine kinase in a two-component regulatory system that regulates phosphorus metabolic pathways and undertakes the key mission of information transmission in pathogenic *** full-length phoR gene was successfully cloned from the Vibrio alginolyticus HY9901 strain.A comprehensive analysis of the cloned gene was conducted using *** analysis revealed that the total length of the phoR gene(GenBank accession No.:KJ958404.1)is 1299 bp,with the coding region containing a total of 432 amino acid *** phylogenetic tree of PhoR revealed that it belongs to the same subclade as *** SMART program was employed for the purpose of functional domain prediction,which revealed that PhoR possesses three major functional domains:PAS(amino acids 98-166),HisKA(amino acids 205-272),and HATPase_c(amino acids 317-429).

关键词： Vibrio alginolyticus phoR gene Gene cloning Bioinformatics analysis

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Cloning and Bioinformatics Analysis of hcp Gene in Aeromonas hydrophila

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Asian Agricultural Research 2024年第3期16卷 36-40页

作者： Fan LI Xingjun FU haiyun feng Shi WANG Weijie ZHANG Huanying PANG Na WANG Fisheries College Guangdong Ocean UniversityZhanjiang 524025China Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture&Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes Zhanjiang 524025China Chinese Academy of Inspection and Quarantine Beijing 100176China

[Objectives]To explore the function of hcp gene in Aeromonas hydrophila.[Methods]A pair of specific primers was designed referring to the hcp gene sequence of *** hcp gene was amplified by PCR,and performed bioinformatics analysis.[Results]The hcp gene had a total length of 1650 bp and encoded 549 amino *** theoretical molecular weight of the protein predicted was about 59476.44 *** predicting the N-terminal signal peptide structure of the amino acid sequence,neither obvious signal peptide cleavage site nor signal peptide was found,and the protein had no transmembrane *** amino acid sequence had a N-glycosylation site,4 protein kinase C phosphorylation sites,7 casein kinase II phosphorylation sites,9 N-myristoylation sites,4 isoprene binding sites,10 microbody C-terminal target signal sites,and an ATP/GTP binding site motif A(P-ring).The amino acid sequence of hcp gene of *** was performed homology analysis with other Aeromonas strains,and it showed higher homology with *** the secondary structure,theα-helix,β-sheet,random coil and extended strand accounted for 45.36%,6.01%,37.52%and 11.11%,*** tertiary structure model consisted of 18α-helix and 22β-*** of protein-protein network interaction demonstrated that the proteins interacting with Hcp protein were AHA_3407,nrfA,nirB-1,nirB-2 and AHA_1112.[Conclusions]Through the bioinformatics prediction results,the basic information of hcp gene of *** is preliminarily understood,and the possible function of this protein is predicted,in order to provide guidance for subsequent vaccine research.

关键词： Aeromonas hydrophila Gene amplification hcp gene Bioinformatics analysis

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Molecular Cloning and Bioinformatics Analysis of crp Gene in Vibrio alginolyticus

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Agricultural Biotechnology 2022年第2期11卷 1-5,8页

作者： Zhiqing WEI Weijie ZHANG Linlin YIN haiyun feng Junlin WANG Fuyuan ZENG Xing XIAO Huanying PANG College of Fisheries Guangdong Ocean UniversityGuangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals/Key Laboratory of Diseases Controlling for Aquatic Economic Animals of Guangdong Higher Education InstitutionsZhanjiang 524088China

[Objectives]The crp gene in Vibrio alginolyticus was studied.[Methods]The crp gene of *** HY9901 was cloned and analyzed by bioinformatics.[Results]The ORF of the crp gene is 633 bp long and the predicted amino acid sequence encompasses 210 amino acid *** physicochemical property analysis indicated that the chemical formula of CRP is C_(1051)H_(1704)N_(290)O_(308)S_(10) with a molecular weight of 23.6514 kDa,and its theoretical pI is ***,the protein is stable and *** protein had three protein kinase C phosphorylation site,three casein kinase II phosphorylation site,one N-terminal myristoylation *** BLAST analysis on the sequence revealed high homology with CRPs in other Vibrio species,and particularly the sequence shares about a homology of 99.52%with the CRP in *** SWISS-MODEL software simulated the subunit tertiary structural model of the CRP,and the similarity with template 3hif.1.A was 95.71%.[Conclusions]This study provides a reference for the search for efficient protective antigens against vibrosis.

关键词： Vibrio alginolyticus crp Bioinformatics analysis

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Luminescent metal-halide perovskites:fundamentals,synthesis,and light-emitting devices

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Science China Chemistry 2024年第6期67卷 1776-1838页

作者： Kang Wang Jeong Hui Kim Jie Yang Xiaoke Liu Yixuan Dou Yuxuan Li Weijian Tao haiyun Dong Haiming Zhu Kaifeng Wu Li Na Quan feng Gao Jianpu Wang Letian Dou Yong Sheng Zhao Beijing National Laboratory for Molecular Sciences(BNLMS) Key Laboratory of PhotochemistryInstitute of ChemistryChinese Academy of SciencesBeijing100190China Davidson School of Chemical Engineering Purdue UniversityWest LafayetteIN47907USA School of Chemical Sciences University of Chinese Academy of SciencesBeijing100049China Key Laboratory of Flexible Electronics(KLOFE) Institute of Advanced Materials(IAM)&School of Flexible Electronics(Future Technologies)Nanjing Tech University(NanjingTech)Nanjing211816China Department of Physics Linköping UniversityLinköping58183Sweden Department of Chemistry Virginia TechBlacksburgVA24060USA State Key Laboratory of Molecular Reaction Dynamics Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023China Department of Chemistry Zhejiang UniversityHangzhou310058China Changzhou University Changzhou213164China Department of Chemistry Purdue UniversityWest LafayetteIN47907USA

Metal-halide perovskites have garnered considerable research attention as highly efficient light emitters in recent years due to their outstanding optoelectronic properties with remarkable tunability and excellent solution *** advancements have been achieved in the development of novel halide perovskites,and the exploitations of these materials in lightemitting *** review comprehensively outlines recent breakthroughs in metal-halide perovskites,encompassing the rational design of perovskite materials with tunable light emission properties,the controllable growth of single crystal for a deeper understanding of their structure-property relationships,as well as the fundamental insights into the photophysics and carrier dynamics in perovskite ***,it provides an overview of recent applications of perovskite materials in high-performance light-emitting diodes(LEDs)and lasers.

关键词： metal-halide perovskites perovskite luminescent materials perovskite synthesis excited-state processes perovskite light-emitting diodes perovskite lasers

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双装药多层箱体内部爆炸的破坏效应试验研究

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北京理工大学学报 2022年第4期42卷 331-339页

作者：胡宏伟肖川冯海云宋浦西安近代化学研究所陕西西安710065 中国兵器科学院北京100089

为了研究2个装药不同箱体结构内部同时爆炸的破坏效应,利用模拟箱体结构,进行了相同质量TNT装药在单箱体和4层箱体内部爆炸试验,分析了装药质量和箱体容积的匹配关系以及双装药布放位置对4层箱体破坏效果的影响.结果表明,整体装药单层... 详细信息

为了研究2个装药不同箱体结构内部同时爆炸的破坏效应,利用模拟箱体结构,进行了相同质量TNT装药在单箱体和4层箱体内部爆炸试验,分析了装药质量和箱体容积的匹配关系以及双装药布放位置对4层箱体破坏效果的影响.结果表明,整体装药单层箱体内部爆炸时,箱体撕裂、抛掷严重,结构整体毁伤效果差;以箱体的破坏数量为基准,2个装药(每个装药的质量为整体装药的1/2)在双箱内部爆炸的毁伤效果都优于相同质量整体装药单箱内部爆炸.装药位置导致的能量叠加和扩散对多箱体的毁伤效果影响很大,整体装药的较佳爆炸位置为中间箱体,双装药的较佳爆炸位置为中间的相邻箱体或隔箱,相隔一个箱体破坏效果最好.

关键词：爆炸力学多层箱体双装药内爆炸破坏效应

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Cloning and Bioinformatics Analysis of vscB Gene of T3SS Chaperone of Vibrio alginolyticus

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Asian Agricultural Research 2023年第4期15卷 37-39,46页

作者： Hongwei ZHENG Liangchuan CHEN haiyun feng Yunsheng CHANG Yu DING Weijie ZHANG Huanying PANG Na WANG Shenzhen Institute of Guangdong Ocean University Shenzhen 510000China Fisheries College Guangdong Ocean UniversityZhanjiang 524025China Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture&Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes Zhanjiang 524025China Chinese Academy of Inspection and Quarantine Beijing 100176China

[Objectives]To clone and analyze the vscB gene of Vibrio alginolyticus HY9901 by bioinformatics.[Methods]A pair of specific primers were designed according to the vscB gene sequence of Vibrio alginolyticus *** full length of the primers was cloned by PCR and analyzed by bioinformatics.[Results]The vscB gene was 429 bp long,encoding 142 amino acids,with a theoretical molecular weight of 16.4 kDa and a pI value of *** acid sequence analysis of VscB showed that VscB was not a secretory protein,without signal peptide and transmembrane region,and there were protein kinase C phosphorylation site and casein kinase II phosphorylation site in the *** comparison of amino acid sequences showed that VscB of *** had the highest protein similarity with Vibrio Parahaemolyticus,reaching 91%.Phylogenetic tree analysis showed that the corresponding proteins of *** VscB,Vibrio Parahaemolyticus and Vibrio diabolicus were clustered in the same *** domain analysis showed that it had CesT family *** structure prediction showed that there were 3α-helices and 5β-turns in VscB protein.[Conclusions]This study provided a theoretical basis for further study on the function of chaperone of ***.

关键词： Vibrio alginolyticus Gene cloning vscB Bioinformatics analysis

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非理想炸药在混凝土介质中的爆炸做功特性

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爆炸与冲击 2018年第1期38卷 197-203页

作者：胡宏伟冯海云陈朗顾晓辉宋浦西安近代化学研究所燃烧与爆炸技术重点实验室陕西西安710065 北京理工大学机电学院北京100081 南京理工大学机械工程学院江苏南京210094

为了研究非理想炸药的做功能力,对TNT、PBXN-109、AFX-757和CL-20基炸药进行了混凝土介质内爆炸实验,测量了实验炸药爆炸形成的混凝土腔体容积,通过量纲分析和实验数据建立了混凝土腔体容积的计算模型,并采用自行设计的混凝土腔体容积... 详细信息

为了研究非理想炸药的做功能力,对TNT、PBXN-109、AFX-757和CL-20基炸药进行了混凝土介质内爆炸实验,测量了实验炸药爆炸形成的混凝土腔体容积,通过量纲分析和实验数据建立了混凝土腔体容积的计算模型,并采用自行设计的混凝土腔体容积方法评价了炸药的爆炸做功特性。结果表明,混凝土腔体容积法能够用于评估非理想炸药的做功能力,单位质量同类型炸药的混凝土腔体容积基本一致。混凝土腔体容积与炸药能量(或炸药质量与爆热的乘积)存在线性关系,炸药混凝土中内爆炸的相对做功能力可以通过爆热当量确定。

关键词：非理想炸药混凝土做功能力爆热量纲分析

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水下爆炸气泡脉动周期的简便计算方法

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高压物理学报 2022年第1期36卷 185-191页

作者：段超伟宋浦胡宏伟冯海云西安近代化学研究所陕西西安710065

将爆轰产物状态方程与气泡运动方程相结合,提出了一种水下爆炸气泡脉动周期的数值计算方法,能够快速计算不同炸药水下爆炸的气泡脉动周期。结果表明:采用JWL状态方程与采用γ律状态方程的气泡周期计算方法相比,前者计算TNT炸药水下爆炸... 详细信息

将爆轰产物状态方程与气泡运动方程相结合,提出了一种水下爆炸气泡脉动周期的数值计算方法,能够快速计算不同炸药水下爆炸的气泡脉动周期。结果表明:采用JWL状态方程与采用γ律状态方程的气泡周期计算方法相比,前者计算TNT炸药水下爆炸气泡脉动周期的误差更小,计算误差小于3%;通过对比RS211炸药水下爆炸实验结果,进一步验证了该数值计算方法对含铝炸药水下爆炸气泡脉动周期的计算同样具有良好的适用性。

关键词：水下爆炸气泡脉动周期状态方程数值计算方法

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桥梁工程施工过程中桥面裂缝的防治

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山西建筑 2016年第21期42卷 183-184页

作者：冯海云山西路桥集团第二工程有限公司山西临汾041000

介绍了桥梁工程中,桥面裂缝的种类及形成原因,从施工材料、混凝土养护、施工工艺等方面,阐述了桥面裂缝的防治措施,旨在提高桥梁的施工质量,延长桥梁的使用年限。

关键词：桥梁桥面裂缝混凝土施工工艺

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