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Against all odds:The road to success in the development of human immune reconstitution mice

Animal Models and Experimental Medicine 2024年第4期7卷 460-470页

作者： Yixiao Bin Jing Ren Haowei Zhang Tianjiao Zhang Peijuan Liu Zhiqian Xin haijiao yang Zhuan Feng Zhinan Chen Hai Zhang School of Basic Medical Sciences Shaanxi University of Chinese MedicineXianyangChina Department of Cell Biology National Translational Science Center for Molecular MedicineFourth Military Medical UniversityXi'anChina State Key Laboratory of New Targets Discovery and Drug Development for Major Diseases Fourth Military Medical UniversityXi'anChina Department of Occupational&Environmental Health and the Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment School of Public HealthFourth Military Medical UniversityXi'anChina

The mouse genome has a high degree of homology with the human genome,and its physiological,biochemical,and developmental regulation mechanisms are similar to those of humans;therefore,mice are widely used as experimental ***,it is undeniable that interspecies differences between humans and mice can lead to experimental *** differences in the immune system have become an impor-tant factor limiting current immunological *** application of immunodefi-cient mice provides a possible solution to these *** transplanting human immune cells or tissues,such as peripheral blood mononuclear cells or hematopoietic stem cells,into immunodeficient mice,a human immune system can be reconstituted in the mouse body,and the engrafted immune cells can elicit human-specific immune *** have been actively exploring the development and differen-tiation conditions of host recipient animals and grafts in order to achieve better im-mune *** genetic engineering methods,immunodeficient mice can be further modified to provide a favorable developmental and differentiation microenvironment for the *** initially only being able to reconstruct single T lymphocyte lineages,it is now possible to reconstruct lymphoid and myeloid cells,providing important research tools for immunology-related *** this review,we compare the differences in immune systems of humans and mice,describe the devel-opment history of human immune reconstitution from the perspectives of immuno-deficient mice and grafts,and discuss the latest advances in enhancing the efficiency of human immune cell reconstitution,aiming to provide important references for im-munological related researches.

关键词： hematopoietic stem cell human immune reconstitution immune response immunodeficient mice peripheral blood mononuclear cell transplantation

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下调HMGB2表达对肝癌LM3细胞上皮-间质转化的抑制作用及其AKT/mTOR信号通路机制

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吉林大学学报（医学版） 2024年第1期50卷 143-149页

作者：魏雁虹杨晨雪杨广民宋帅李明杨海娇魏海峰吉林省人民医院医学检验中心吉林长春130021 吉林省人民医院生物治疗与基因诊断重点实验室吉林长春130021

目的:探讨下调肝癌细胞中高迁移率族框蛋白2 (HMGB2)表达对肝癌细胞生物学行为及上皮-间质转化(EMT)进程的影响,并阐明其作用机制。方法:对数生长期的人肝癌LM3细胞分为阴性对照组和HMGB2 RNA干扰组(HMGB2 siRNA组),分别以Lipofectamin ... 详细信息

目的:探讨下调肝癌细胞中高迁移率族框蛋白2 (HMGB2)表达对肝癌细胞生物学行为及上皮-间质转化(EMT)进程的影响,并阐明其作用机制。方法:对数生长期的人肝癌LM3细胞分为阴性对照组和HMGB2 RNA干扰组(HMGB2 siRNA组),分别以Lipofectamin 2000为载体转染无关序列的RNA寡核苷酸(RNA oligo)和敲除HMGB2序列的RNA oligo。采用实时荧光定量PCR(RT-qPCR)法和Western blotting法检测2组细胞中HMGB2 mRNA和蛋白表达水平,分别采用细胞划痕实验和Transwell小室实验检测2组细胞的迁移和侵袭能力,采用Western blotting法检测2组细胞中E-钙黏蛋白(E-cadherin)、 N-钙黏蛋白(N-cadherin)、波形蛋白(Vimentin)和蛋白激酶B(AKT)/哺乳动物雷帕霉素靶蛋白(mTOR)通路相关蛋白表达水平。结果:与阴性对照组比较,HMGB2 siRNA组细胞中HMGB2 mRNA和蛋白表达水平均明显降低(P<0.05),HMGB2 siRNA组细胞划痕愈合率明显降低(P<0.01),侵袭细胞数明显减少(P<0.01),细胞中E-cadherin蛋白表达水平明显升高(P<0.01),N-cadherin、Vimentin、mTOR、AKT和磷酸化AKT (p-AKT)蛋白表达水平明显降低(P<0.05或P<0.01)。结论:下调HMGB2的表达可降低肝癌LM3细胞迁移和侵袭能力并抑制EMT,其作用机制可能与参与调节AKT/mTOR通路相关蛋白表达有关。

关键词：肝肿瘤高迁移率族框蛋白2 上皮-间质转化细胞迁移细胞侵袭蛋白激酶B/哺乳动物雷帕霉素靶蛋白

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八正片联合坦洛新片治疗输尿管壁内段结石的效果

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西北药学杂志 2024年第1期39卷 171-175页

作者：宋群杨海蛟蒋颖李卓湖南省人民医院泌尿外科长沙410000 石门县中医医院泌尿外科常德415000

目的探讨八正片联合坦洛新片治疗输尿管壁内段结石的临床疗效。方法选取确诊输尿管壁内段结石的患者93例,其中90例患者符合纳入标准,用随机分组法分为对照组(n=45)和观察组(n=45)。2组患者采用体外冲击波碎石后,对照组患者口服坦洛新片... 详细信息

目的探讨八正片联合坦洛新片治疗输尿管壁内段结石的临床疗效。方法选取确诊输尿管壁内段结石的患者93例,其中90例患者符合纳入标准,用随机分组法分为对照组(n=45)和观察组(n=45)。2组患者采用体外冲击波碎石后,对照组患者口服坦洛新片,观察组口服八正片联合坦洛新片,均持续治疗15 d。治疗后,评估2组患者的临床疗效。比较2组中医证候积分及结石排净率;统计2组肾绞痛发作率、抗生素使用率、止痛药使用率、结石排净时间、抗生素使用时间及视觉模拟疼痛(visual analogue scale,VAS)评分;比较2组患者治疗前后的肾功能指标(肌酐、尿素氮)及尿生化指标(尿酸、尿草酸和尿钙)水平。记录2组治疗期间并发症发生情况。结果观察组的治疗有效率(95.56%)高于对照组(82.22%),P0.05)。结论八正片联合坦洛新片治疗输尿管壁内段结石疗效显著,可缓解症状,提高结石排净率。

关键词：八正片坦洛新片输尿管壁内段结石体外冲击波碎石

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Response of understory plant species richness and tree regeneration to thinning in Pinus tabuliformis plantations in northern China

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Forest Ecosystems 2023年第2期10卷 215-226页

作者： haijiao yang Chao Pan Yun Wu Shiqi Qing Zhibin Wang Dihai Wang College of Forestry Northwest A&F UniversityYanglingShaanxi712100China

Background:Chinese pine(Pinus tabuliformis Carr.)is one of the major afforestation species in northern China and plays a key role in restoring forest ecosystems and preserving soil and ***,most Chinese pine plantations are experiencing ecological problems such as the low diversity of understory plants and difficulty in natural *** has been widely used to maintain and improve a variety of forest ecosystem services from *** date,however,few studies have been conducted to systematically determine the effects of thinning on understory plant diversity and the regeneration of Chinese pine in ***:We conducted a literature search,and selected 22 publications covering a total of 83 treatments related to thinning effects on the species richness of understory plants and 15 publications covering a total of 43 treatments related to thinning effects on the regeneration of Chinese pine,in tree plantations of northern *** data from the literature were synthesized and evaluated with meta-analysis approach to determine the treatment ***:Compared with the control stands,thinning increased the species richness of shrubs and herbs by an average of 25.3%and 26.5%,*** the varying thinning intensities all had significantly positive effects on the species richness of understory plants,only moderate thinning(30%–50%)had a positive effect on the density of regenerating seedlings and saplings of Chinese pine(60.2%).The species richness of understory plants was greatest after 14 years of thinning with an increase of 36.3%,whereas the density of regenerating Chinese pine seedlings and saplings reached a maximum after≥11 years of thinning with an increase of 76.5%,compared to that of the unthinned *** in the half-mature plantations had the greatest effects on the understory shrub richness(44.1%)and the density of regenerating Chinese pine seedlings and saplings(86.5%).Both single and multiple thinn

关键词： Chinese pine plantation Forest thinning Meta-analysis Natural regeneration Understory plants

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Species composition and influencing factors of understory woody species in Robinia pseudoacacia plantations on the Loess Plateau

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林业研究:英文版 2023年第6期34卷 1693-1706页

作者： haijiao yang Jinfang Gao Chao Pan Shiqi Qing Yun Wu Lina Jiang Zhibin Wang Dihai Wang College of Forestry Northwest A&F UniversityYangling 712100ShaanxiPeople’s Republic of China

Black locust(Robinia pseudoacacia L.)plantations have contributed significantly to soil and water conservation and ecological reconstruction on China’s Loess *** the impact of stand and environment on species composition of understory woody plants will improve the stability of existing black locust *** stands were selected in second-generation black locust plantations in tableland and gully areas of the Loess *** number of understory tree species in the tablelands was significantly lower than in the gully *** black locust(19.76%)and Rubus corchorifolius L.f.(64.85%)were the most abundant understory tree and shrub species,respectively,in the tableland stands;Broussonetia papyrifera(L.)L’Hé*** Vent.(6.77%)and Acanthopanax senticosus(***.)Harms.(37.22%)were most abundant in the gully *** richness(S),Shannon diversity(H),and evenness index(J)of the understory plants were significantly lower in the tableland stands than in the gully *** diverse understory species and community structures occurred in the gully *** in species diversity among landform positions may be attributed to differences in soil *** addition,77.57%of the variation in understory species composition was explained,among which shrub and herb coverage,stand age,leaf area index,slope and total soil phosphorus in the 10-20 cm layer were the main *** organic carbon and total potassium significantly impacted S,H and *** the environmental conditions and the biological characteristics of the plants investigated,*** should be given priority in the development of tableland stands,while *** and Celtis sinensis *** form mixed forests with black locust in gully *** management could promote biodiversity and stability of the existing black locust plantations but also optimize regional landscape patterns.

关键词： Black locust plantation Understory woody plants Species composition Species diversity pattern Loess plateau

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人工湿地发展现状

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林业科技情报 2024年第2期56卷 125-127页

作者：马晓龙赵雪涛杨海娇石坤冉善水博通(宁夏)环境科技有限公司银川750001

作为水环境保护体系的重要角色,人工湿地在我国发展迅速,被广泛应用于各种污水体系建设。该文系统总结了人工湿地研究的发展现状,梳理了近年来国内外人工湿地类型及优缺点现状。并且针对人工湿地的植物、基质等主要组成部分的成果做研... 详细信息

作为水环境保护体系的重要角色,人工湿地在我国发展迅速,被广泛应用于各种污水体系建设。该文系统总结了人工湿地研究的发展现状,梳理了近年来国内外人工湿地类型及优缺点现状。并且针对人工湿地的植物、基质等主要组成部分的成果做研究分析,旨为今后的人工湿地设计、建设和运维提供技术参考。

关键词：人工湿地现状类型植物选型基质

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蓝靛果花青素降血糖作用及机制

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食品研究与开发 2024年第18期45卷 60-67页

作者：曹柏营王家利侯宇杨海蛟于佳鑫钟雪松吉林工程技术师范学院生物与食品工程学院吉林长春130052 吉林省产品质量监督检验院吉林长春130103 敦煌市市场监督管理局甘肃敦煌736200

以蓝靛果花青素为原料,评价蓝靛果花青素的体内外降血糖作用,从IRS1⁃PI3K⁃AKt信号通路关键酶和细胞因子调控角度探讨蓝靛果花青素的降血糖作用机制。实验结果表明,蓝靛果花青素对α⁃葡萄糖苷酶和α⁃淀粉酶的活性产生抑制作用,抑制率分别... 详细信息

以蓝靛果花青素为原料,评价蓝靛果花青素的体内外降血糖作用,从IRS1⁃PI3K⁃AKt信号通路关键酶和细胞因子调控角度探讨蓝靛果花青素的降血糖作用机制。实验结果表明,蓝靛果花青素对α⁃葡萄糖苷酶和α⁃淀粉酶的活性产生抑制作用,抑制率分别为76.92%和74.85%。蓝靛果花青素可降低糖尿病小鼠血糖水平(与模型组相比,高剂量组降低26.01%),降低糖耐量值(与模型组相比,高剂量组降低25.98%),提高肝脏糖原的储存能力,提高己糖激酶(hexokinase,HK)活性,抑制葡萄糖⁃6⁃磷酸酶(glucose⁃6⁃phosphatase,G⁃6⁃Pase)和磷酸烯醇丙酮酸羧激酶(phos⁃phoenolpyruvate carboxykinase,PEPCK)活性,下调FoxO1、G6PC、PEPCK和GSK‐3βmRNA表达量,上调IRS1、PI3K和AKt mRNA表达量,通过激活IRS1⁃PI3K⁃AKt信号通路,抑制糖异生过程,调节糖代谢紊乱,发挥降血糖作用。

关键词：蓝靛果花青素降血糖胰岛素抵抗 IRS1⁃PI3K⁃AKt信号通路

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Light/pH dual-responsive magnetic metal-organic frameworks composites for phosphorylated peptide enrichment

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Chinese Chemical Letters 2024年第7期35卷 222-226页

作者： Rui Wang He Qi haijiao Zheng Qiong Jia College of Chemistry Jilin UniversityChangchun 130012China Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education School of Life SciencesJilin UniversityChangchun 130012China

Metal-organic frameworks(MOFs)combined with specific ligands are highly adaptable smart materials that can respond to external and physiological *** this study,we introduced a pyridinyl zwitterionic ligand with light/pH dual response into magnetic MOF composite(Fe_(3)O_(4)@ZW-MOF)for enrichment of phosphorylated peptides for the first *** introduction of the developed ligand gives MOF material dual response *** stimulation affects the generation/disappearance of free radicals of the pyridine derivative,resulting in a change in the charge gradient of the zwitterion,and zwitterion can also regulate the p H of the solution by adding acid or ***,the reversible capture and release of phosphorylated peptides can be easily achieved by adjusting light and *** established phosphorylated peptide enrichment platform exhibits high sensitivity(detection limit of 1 fmol),high selectivity(β-casein:BSA,1:1000),and good reusability(7 cycles).In addition,the method was applied to the enrichment of phosphorylated peptides in complex systems(non-fat milk and human serum),demonstrating the feasibility of this method for phosphoproteom *** conclusion,the synthesized Fe_(3)O_(4)@ZW-MOF is a promising MOF material,which provides the possibility to advance the application of responsive MOFs materials in proteomics.

关键词： Light pH Dual-response Metal-organic frameworks Phosphorylated peptide

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蓝靛果花青素抗氧化作用及机制

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食品研究与开发 2024年第19期45卷 53-59页

作者：曹柏营杨海蛟侯宇王家利乔新宇陈英伟刘馨阳吉林工程技术师范学院生物与食品工程学院吉林长春130052 敦煌市市场监督管理局甘肃敦煌736200 吉林省产品质量监督检验院吉林长春130103

以蓝靛果花青素为原材料,评价蓝靛果花青素的体外抗氧化作用,从Nrf2-Keap1-ARE通路关键因子的调控角度探讨蓝靛果花青素的抗氧化机制。结果表明,蓝靛果花青素对DPPH自由基、ABTS+自由基和羟自由基的清除率分别为92.48%、99.82%、95.67%... 详细信息

以蓝靛果花青素为原材料,评价蓝靛果花青素的体外抗氧化作用,从Nrf2-Keap1-ARE通路关键因子的调控角度探讨蓝靛果花青素的抗氧化机制。结果表明,蓝靛果花青素对DPPH自由基、ABTS+自由基和羟自由基的清除率分别为92.48%、99.82%、95.67%,总抗氧化能力为186.62 U/mg,蓝靛果花青素可提高H2O2诱导Caco-2细胞损伤的超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)和谷胱甘肽过氧化物酶(glu-tathione per-oxidase,GSH-Px)活性,降低丙二醛(malonyldialdehyde,MDA)水平,上调Nrf2-Keap1-ARE通路中的核因子E2相关因子2(nuclear factor erythroid 2-related factor 2,Nrf2)mRNA表达量,上调血红素加氧酶l(heme oxygenase,HO-1)和醌羟基氧化还原酶l(quinone oxidoreductase 1,NQO1)mRNA表达量,下调Kelch样ECH关联蛋白1(recombinant kelch Like ECH associated protein 1,Keap1)mRNA表达量,激活细胞抗氧化酶的活性,发挥氧化损伤的保护作用,抗氧化作用与维生素C(vitamin,VC)和特丁基对苯二酚(tert-butylhydroquinone,t-BHQ)相当。

关键词：蓝靛果花青素抗氧化活性氧化应激 Nrf2-Keap1-ARE信号通路

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Interfacial growth of N,S-codoped mesoporous carbon onto biomass-derived carbon for superior potassium-ion storage

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Nano Research 2024年第4期17卷 2619-2627页

作者： Na Huang Cheng Tang Hao Jiang Jie Sun Aijun Du haijiao Zhang Institute of Nanochemistry and Nanobiology School of Environmental and Chemical EngineeringShanghai UniversityShanghai 200444China School of Chemistry and Physics and Centre for Materials Science Queensland University of TechnologyBrisbaneQLD 4001Australia Shanghai Engineering Research Center of Hierarchical Nanomaterials Frontiers Science Center for Materiobiology and Dynamic ChemistrySchool of Materials Science and EngineeringEast China University of Science and TechnologyShanghai 200237China

Carbonaceous materials have been recognized as one of the most promising anode materials for potassium-ion batteries(PIBs)due to their abundant raw materials,controllable structure,superior conductivity,and good chemical ***,the large radius of K ions and the low potassium content of intercalation compounds result in the sluggish storage kinetics and low reversible capacity of carbon *** this work,we present a unique heteroatom-doped carbon composite(denoted as NS-MC/SC)through a facile interfacial assembly route and simple heat-treatment process,where NS-MC is well grafted onto the biomass-derived spore carbon(SC).This unique structural design endows it with abundant mesoporous channels,expanded layer spacing,and highly doped N and *** these merits,the NS-MC/SC anode in PIBs exhibits a high reversible capacity of 350.4 mAh·g^(-1) at 100 mA·g^(-1) after 300 cycles,and an outstanding cycling ***,in-situ Raman spectra further verify the high reversibility of K ions insertion/***,theoretical simulations also reveal that the N,S dual-doping is an efficient approach for improving the potassium-ion storage performance of NS-MC/SC.

关键词： mesoporous carbon(MC) biomass-derived carbon(BC) N S co-doping interfacial assembly potassium-ion batteries(PIBs)

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