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HSV-1 H129-Derived Anterograde Neural Circuit Tracers:Improvements, Production, and Applications

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Neuroscience Bulletin 2021年第5期37卷 701-719页

作者： Hong Yang Feng Xiong Yi-Ge Song hai-fei jiang hai-Bin Qin Jing Zhou Sha Lu Steven FGrieco Xiangmin Xu Wen-Bo Zeng fei Zhao Min-Hua Luo State Key Laboratory of Virology CAS Center for Excellence in Brain Science and Intelligence TechnologyCenter for Biosafety Mega-ScienceWuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of Chinese Academy of Sciences Beijing 100049China Shanghai Genechem Co.Ltd.Shanghai 201203 China Department of Anatomy and Neurobiology School of MedicineUniversity of CaliforniaIrvineCA 92697USA School of Basic Medical Sciences Capital Medical UniversityBeijing 100069China Chinese Institute for Brain Research Beijing 102206China Shanghai Public Health Clinical Center Fudan UniversityShanghai 200032China

Anterograde viral tracers are powerful and essential tools for dissecting the output targets of a brain region of interest. They have been developed from herpes simplex virus 1(HSV-1) strain H129(H129), and have been successfully applied to map diverse neural ***, the anterograde polysynaptic tracer H129-G4 was used by many groups. We then developed the first monosynaptic tracer, H129-dTK-tdT, which was highly successful, yet improvements are needed. Now, by inserting another tdTomato expression cassette into the H129-dTK-tdT genome, we have created H129-dTK-T2, an updated version of H129-dTK-tdT that has improved labeling intensity. To help scientists produce and apply our H129-derived viral tracers, here we provide the protocol describing our detailed and standardized procedures. Commonly-encountered technical problems and their solutions are also discussed in detail. Broadly, the dissemination of this protocol will greatly support scientists to apply these viral tracers on a large scale.

关键词： HSV-1 strain H129(H129) Anterograde transneuronal tracer Polysynaptic tracer Monosynaptic tracer Production Application Neural circuit Neural circuit tracing

Anterograde Monosynaptic Transneuronal Tracers Derived from Herpes Simplex Virus 1 strain H129

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Anterograde Monosynaptic Transneuronal Tracers Derived from ...

Crystallization Modulation and Holistic Passivation Enables Efficient Two‑Terminal Perovskite/CuIn(Ga)Se_(2)Tandem Solar Cells

中国神经科学学会第十二届全国学术会议

作者： Wen-Bo Zeng hai-fei jiang Yousheng Shu Shao-Qun Zeng Fuqiang Xu fei Zhao Min-Hua Luo State Key Laboratory of Virology CAS Center for Excellence in Brain Science and Intelligence Technology (CEBSIT) Wuhan Institute of Virology Chinese Academy of Sciences State Key Laboratory of Membrane Biology School of Life Sciences Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics Brain Research Center Wuhan Institute of Physics and Mathematics Chinese Academy of Sciences

Objective Herpes simplex virus type 1 strain 129（H129） has represented a promising anterograde neuronal circuit tracing tool, which complements the existing retrograde tracers. However, the current H129 derived tracers are multisynaptic, neither bright enough to label the details of neurons nor capable of determining direct projection targets as monosynaptic tracer. The present study is to introduce an improved multisynaptic anterograde tracer H129-G4 with strong labeling intensity, and a novel mono-synaptic anterograde tracer H129-ΔTK-td T. Methods Based on the bacterial artificial chromosome of H129, we have generated a serial of recombinant viruses for neuronal circuit tracing. Among them, H129-G4 was obtained by inserting binary tandemly connected GFP cassettes into the H129 genome, and H129-ΔTK-td T was obtained by deleting the thymidine kinase（TK） gene and adding td Tomato coding gene to the H129 genome. Then the obtained viral tracers were tested in vitro and in vivo for the tracing capacity. Results H129-G4 is capable of transmitting through multiple synapses, labeling the neurons by green florescent protein, and visualizing the morphological details of the labeled neurons. H129-ΔTK-td T neither replicates nor spreads in neurons alone, but transmits to and labels the postsynaptic neurons with td Tomato in the presence of complementary expressed TK from a helper virus. H129-ΔTK-td T is also capable to map the direct projectome of the specific neuron type in the given brain regions in Cre transgenic mice. In the tested brain regions where circuits are well known, the H129-ΔTK-td T tracing patterns are consistent with the previous results. Conclusion With the assistance of the helper virus complimentarily expressing TK, H129-ΔTK-td T replicates in the initially infected neuron, transmits anterogradely through one synapse, and labeled the postsynaptic neurons with td Tomato. The H129-ΔTK-td T anterograde monosynaptic tracing system offers a useful tool for mappi

关键词： Herpes simplex virus type 1(HSV-1) H129 strain anterograde neuronal tracer H129-ΔTK-tdT monosynaptic H129-G4 multisynaptic

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Nano-Micro Letters 2025年第1期17卷 205-215页

作者： Cong Geng Kuanxiang Zhang Changhua Wang Chung Hsien Wu Jiwen jiang fei Long Liyuan Han Qifeng Han Yi‑Bing Cheng Yong Peng State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan 430070People’s Republic of China Triumph Photovoltaic Materials Co. Ltd.No.1001 Yannan AvenueHigh‑Tech Development DistrictBengbuAnhui 233000People’s Republic of China College of Materials Science and Engineering Guilin University of TechnologyGuilin 541004People’s Republic of China State Key Laboratory of Metal Matrix Composites Shanghai Jiao Tong UniversityShanghai 200240People’s Republic of China

Two-terminal(2-T)perovskite(PVK)/CuIn(Ga)Se_(2)(CIGS)tandem solar cells(TSCs)have been considered as an ideal tandem cell because of their best bandgap matching regarding to Shockley–Queisser(S–Q)***,the nature of the irregular rough morphology of commercial CIGS prevents people from improving tandem device *** this paper,D-homoserine lactone hydrochloride is proven to improve coverage of PVK materials on irregular rough CIGS surfaces and also passivate bulk defects by modulating the growth of PVK *** addition,the minority carriers near the PVK/C60 interface and the incompletely passivated trap states caused interface recombination.A surface reconstruction with 2-thiopheneethylammonium iodide and N,N-dimethylformamide assisted passivates the defect sites located at the surface and grain ***,LiF is used to create this field effect,repelling hole carriers away from the PVK and C60 interface and thus reducing *** a result,a 2-T PVK/CIGS tandem yielded a power conversion efficiency of 24.6%(0.16 cm^(2)),one of the highest results for 2-T PVK/CIGS TSCs to our *** validation underscores the potential of our methodology in achieving superior performance in PVK/CIGS tandem solar cells.

关键词： PVK/CIGS TSCs Irregular rough surfaces Modulating the growth PVK/C60 interface recombination

In Situ Observation of Gypsum-Anhydrite Transition at High Pressure and High Temperature

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Chinese Physics Letters 2012年第4期29卷 252-255页

作者： LIU Chuan-jiang ZHENG hai-fei Key Laboratory of Orogenic Belts and Crustal Evolution(Ministry of Education) Peking UniversityBeijing 100871 Institute of Geochemistry Peking UniversityBeijing 100871

An in-situ Raman spectroscopic study of gypsum-anhydrite transition under a saturated water condition at high pressure and high temperature is performed using a hydrothermal diamond anvil cell (HDAC).The experimental results show that gypsum dissolvs in water at ambient temperature and above 496 *** increasing temperature,the anhydrite (CaSO4) phase precipitates at 250 320℃ in the pressure range of 1.0 1.5 GPa,indicating that under a saturated water condition,both stable conditions of pressure and temperature and high levels of Ca and SO4 ion concentrations in aqueous solution are essential for the formation of anhydrite.A linear relationship between the pressure and temperature for the precipitation of anhydrite is established as P(GPa) =0.0068T - 0.7126 (250℃≤T≤320℃).Anhydrite remained stable during rapid cooling of the sample chamber,showing that the gypsum-anhydrite transition involving both dissolution and precipitation processes is irreversible at high pressure and high temperature.

关键词： saturated Transition Observation

Outcome prediction value of National Early Warning Score in septic patients with community-acquired pneumonia in emergency department: A single-center retrospective cohort study

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World Journal of Emergency Medicine 2020年第4期11卷 206-215页

作者： hai-jiang Zhou Tian-fei Lan Shu-bin Guo Emergency Medicine Clinical Research Center Beijing Chao-yang HospitalCapital Medical University&Beijing Key Laboratory of Cardiopulmonary Cerebral ResuscitationBeijingChina Department of Allergy Beijing Shijitan HospitalCapital Medical UniversityBeijingChina

BACKGROUND:To evaluate the accuracy of National Early Warning Score(NEWS)in predicting clinical outcomes(28-day mortality,intensive care unit[ICU]admission,and mechanical ventilation use)for septic patients with community-acquired pneumonia(CAP)compared with other commonly used severity scores(CURB65,Pneumonia Severity Index[PSI],Sequential Organ Failure Assessment[SOFA],quick SOFA[qSOFA],and Mortality in Emergency Department Sepsis[MEDS])and admission lactate ***:Adult patients diagnosed with CAP admitted between January 2017 and May 2019 with admission SOFA≥2 from baseline were *** characteristics were *** primary outcome was the 28-day mortality after admission,and the secondary outcome included ICU admission and mechanical ventilation *** prediction value of parameters above was compared using receiver operating characteristics(ROC)*** regression analyses were carried out to determine the risk factors for the 28-day ***-Meier survival curves were plotted and compared using optimal cut-off values of qSOFA and ***:Among the 340 enrolled patients,90 patients were dead after a 28-day follow-up,62 patients were admitted to ICU,and 84 patients underwent mechanical *** single predictors,NEWS achieved the largest area under the receiver operating characteristic(AUROC)curve in predicting the 28-day mortality(0.861),ICU admission(0.895),and use of mechanical ventilation(0.873).NEWS+lactate,similar to MEDS+lactate,outperformed other combinations of severity score and admission lactate in predicting the 28-day mortality(AUROC 0.866)and ICU admission(AUROC 0.905),while NEWS+lactate did not outperform other combinations in predicting mechanical ventilation(AUROC 0.886).Admission lactate only improved the predicting performance of CURB65 and qSOFA in predicting the 28-day mortality and ICU ***:NEWS could be a valuable predictor in septic patients with CAP in emergency depa

关键词： Community-acquired pneumonia Sepsis National Early Warning Score(NEWS) Intensive care unit Emergency departments

Strength and ductility optimization of HPDC AlSi8MgCuZn2 alloys by modifying pre-aging treatment

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China Foundry 2024年第4期21卷 343-351页

作者： Yuan-hang jiang Hui-ting Zheng fei Liu hai-dong Zhao National Engineering Research Center of Near-net Shape Forming for Metallic Materials South China University of TechnologyGuangzhou 510641China

Considering the components produced by high pressure die casting(HPDC)process usually with ultra-large sizes and complex morphologies,high temperature solid solution treatment is not a suitable method to further improve their mechanical *** this study,two-stage aging treatment with different pre-aging times was designed and employed to further improve the mechanical properties of HPDC Al8SiMgCuZn *** characteristics of precipitates were evaluated by a transmission electron microscope(TEM),and the precipitation strengthening mechanism was *** results reveal that the strengthening is mainly contributed by the precipitation ofβ″phase after two-stage aging,and the number density and size of the precipitates are significantly depended on the pre-aging *** number density of precipitates is increased with the pre-aging time prolonged from 0 h to 4 h,and then decreases with the further increase of pre-aging time from 4 h to 6 *** precipitates with the highest density and smallest size are observed after pre-aging for 4 *** pre-aged at 100℃for 4 h and then artificial aged at 200℃for 30 min,the yield strength of 207 MPa,ultimate tensile strength of 325 MPa and elongation of 7.6%are achieved.

关键词： high pressure die cast aluminum alloy microstructural heterogeneity mechanical properties precipitation strengthening two-stage aging

Design and Analysis of a Novel 2T2R Parallel Mechanism with the Closed-loop Limbs

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International Journal of Automation and computing 2021年第4期18卷 654-666页

作者： hai-Rong Fang Peng-fei Liu Hui Yang Bing-Shan jiang School of Mechanical Electronic and Control EngineeringBeijing Jiaotong UniversityBeijing 100044China

This paper presents a novel four degrees of freedom(DOF) parallel mechanism with the closed-loop limbs, which includes two translational(2 T) DOF and two rotational(2 R) DOF. By connecting the proposed parallel mechanism with the guide rail in series,the 5-DOF hybrid robot system is obtained, which can be applied for the composite material tape laying in aerospace industry. The analysis in this paper mainly focuses on the parallel module of the hybrid robot system. First, the freedom of the proposed parallel mechanism is calculated based on the screw theory. Then, according to the closed-loop vector equation, the inverse kinematics and Jacobian matrix of the parallel mechanism are carried out. Next, the workspace stiffness and dexterity analysis of the parallel mechanism are investigated based on the constraint equations, static stiffness matrix and Jacobian condition number. Finally, the correctness of the inverse kinematics and the high stiffness of the parallel mechanism are verified by the kinematics and stiffness simulation analysis, which lays a foundation for the automatic composite material tape laying.

关键词： 2T2R parallel mechanism close loop structure high stiffness kinematics analysis workspace

早产低出生体质量儿乳牙萌出和相关影响因素的调查研究

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牙体牙髓牙周病学杂志 2012年第12期22卷 718-721页

作者：张锋卢东旭邓文娇李海飞江文刘寿桃深圳市妇幼保健院口腔病防治中心广东深圳518048 深圳市妇幼保健院儿童保健科广东深圳518048

目的:研究早产低出生体质量儿乳牙初萌时间,并追踪观察早产低出生体质量儿多个时间点乳牙萌出的数目,探索影响乳牙萌出的相关因素。方法:选择早产低出生体质量儿35例和足月出生婴儿71例为对象,定期进行口腔检查并记录牙齿初萌时间、萌... 详细信息

目的:研究早产低出生体质量儿乳牙初萌时间,并追踪观察早产低出生体质量儿多个时间点乳牙萌出的数目,探索影响乳牙萌出的相关因素。方法:选择早产低出生体质量儿35例和足月出生婴儿71例为对象,定期进行口腔检查并记录牙齿初萌时间、萌出牙位、萌出数目,统计分析孕周、出生体质量、性别、分娩方式、胎数、喂养方式等因素对乳牙萌出有无影响。结果:①两组最早萌出的牙均为下颌乳前牙,足月儿乳牙初萌明显早于早产低出生体质量儿(P0.05);③早产低出生体质量儿在出生后9个月、12个月时牙齿萌出数目明显低于足月儿(P<0.05);④在影响牙齿萌出的相关因素中,孕周、出生体质量与牙齿初萌年龄呈明显负相关,与牙齿萌出数目明显正相关;而喂养方式、性别、分娩方式以及胎数对乳牙萌出时间没有影响。结论:早产低出生体质量儿乳牙初萌明显较足月儿推迟,但以生理年龄计算,两组婴儿牙齿初萌时间无统计学差异。出生体质量与孕周是影响牙齿初萌时间和牙齿萌出数目的相关因素。

关键词：牙齿萌出乳牙早产低出生体质量婴儿

Deciphering the molecular mechanisms of Simiaowan in the treatment of hyperuricemia: in vivo and in silico approaches

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Traditional Medicine Research 2025年第2期10卷 37-54页

作者： Yong-Chang Zeng Jun-Hong Wu Dan-Dan Xu Kang He Chang-Qing Liu Li-fei Song Zheng-Zhi Wu Qian-Qian jiang Shao-Yu Liang Department of Neurology The First Affiliated Hospital of Shenzhen UniversityShenzhen 518020China Pharmaceutical Department The Affiliated TCM Hospital of Guangzhou Medical UniversityGuangzhou 510000China School of Pharmacy Guizhou University of Traditional Chinese MedicineGuiyang 550025China Guangzhou Zeli Medical Technology Co. Ltd.Guangzhou 510700China

Background:Simiaowan(SMW),a well-known traditional Chinese medicine,has been employed to treat hyperuricemia(HUA)and gout for ***,the bioactive components and underlying mechanisms have not been *** objective of this study was to identify the active components and potential mechanisms of SMW by integrating pharmacological experimentation,serum pharmacochemistry,network pharmacology and molecular ***:HUA rats modelling by high-fat/high-sugar diet and potassium oxonate/adenine oral administration were used to evaluate the pharmacodynamic effects of ***-Q-Exactive-MS/MS was employed to detect the bioactive components present in SMW-containing *** pharmacology and molecular docking were utilized to elucidate the potential targets and underlying ***:SMW effectively ameliorated HUA rats via the inhibition of uric acid(UA)production,promotion of UA excretion,improvement of lipid and glucose metabolic abnormalities,antioxidant,anti-inflammatory and anti-insulin resistance effects.A total of 73 compounds detected in SMW-containing serum were identified as potential active components,with alkaloids,flavonoids,organic acids,and terpenoids emerging as the primary active *** 203 corresponding targets were obtained as SMW anti-HUA/gout targets,which mainly participated in apoptosis,insulin resistance,TNF,PI3K-Akt,HIF-1,NF-κB,MAPK,IL-17 and TLR signaling *** docking indicated that active compounds(***,phellodendrine,quercetin,formononetin,ferulic acid)had superior binding abilities to the key targets(*** carrier family 22 member 12(URAT1),solute carrier family 22 member 6(OAT1),ATP-binding cassette sub-family G member 2(ABCG2),solute carrier family 2,facilitated glucose transporter member 9(GLUT9),xanthine dehydrogenase/oxidase(XDH),transcription factor p65(RELA),toll-like receptor 4(TLR4),prostaglandin G/H synthase 2(PTGS2),caspase-3(CASP3),insulin(INS)).Conclusion:S

关键词： hyperuricemia network pharmacology serum pharmacochemistry Simiaowan UPLC-Q-Exactive-MS/MS