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Application of Biological Nanopore Sequencing Technology in the Detection of Microorganisms

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Chinese Journal of Chemistry 2023年第23期41卷 3473-3483页

作者： Ming-Qian Zhang Xiao-Bin Huang hai-chen wu Beijing National Laboratory for Molecular Sciences Key Laboratory of Analytical Chemistry for Living BiosystemsInstitute of ChemistryChinese Academy of SciencesBeijing100190 China University of Chinese Academy of Sciences Beijing100049 China

Environmental pollution and the spread of pathogenic microorganisms pose a significant threat to the health of humans and the ***,understanding and detecting microorganisms is crucial for maintaining a healthy living *** sequencing is a single-molecule detection method developed in the 1990s that has revolutionized various research *** offers several advantages over traditional sequencing methods,including low cost,label-free,time-saving detection speed,long sequencing reading,real-time monitoring,convenient carrying,and other significant *** this review,we summarize the technical principles and characteristics of nanopore sequencing and discuss its applications in amplicon sequencing,metagenome sequencing,and whole-genome sequencing of environmental microorganisms,as well as its in situ application under some special *** also analyze the advantages and challenges of nanopore sequencing in microbiology ***,nanopore sequencing has the potential to greatly enhance the detection and understanding of microorganisms in environmental research,but further developments are needed to overcome the current challenges.

关键词： Nanopore sequencing technology Environmental microorganisms Amplicon sequencing Metagenome sequencing Whole genome sequencing Gene technology Ion channels Nucleic acids

Selective detection of mercury(II) and methylmercury(II) via coordinationinduced emission of a small-molecule probe

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Science China Chemistry 2016年第12期59卷 1651-1657页

作者： Yan Yang Zhiyi Yao Wei Li Kui chen Lei Liu hai-chen wu Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety Institute of High Energy Physics Chinese Academy of Sciences College of Chemical Engineering Beijing Institute of Petro-chemical Technology

Mercury is one of the major toxic pollutants and has many adverse effects on human health. The main mercury species in the environment or in living organisms are inorganic mercuric ion （Hg2＋） and organic methylmercury （CH3Hg＋）. Detection of the two mercury ions is a particularly active topic in the molecular sensing field during the past decade. However, efficient sensors that can sensitively detect and discriminate the two species are rare. In this work, we adopt the concept of restriction of intramolecular rotations which is the basis of aggregation induced emission, and design a molecular probe with pyridyl group as the chelating unit and 1,8-naphthalimide as the fluorescent unit for the detection of both Hg2＋ and CH3Hg＋. When the probe is free in solution, it exhibits weak fluorescence because free intramolecular rotations of the 1,8-naphthalimide moieties non-radiatively annihilate its excited state. However, upon coordination with Hg2＋ or CH3Hg＋, the rotation of 1,8-naphthalimide moieties would be restricted due to the chelation between 1,8-naphthalimide and Hg2＋ or CH3Hg＋, leading to significantly enhanced fluorescent emission. The response induced by Hg2＋ is much stronger than CH3Hg＋; but for specific detection of CH3Hg＋, we introduced a T-rich DNA fragment which could completely mask Hg2＋ in solution. Furthermore, we have employed the sensor for confocal imaging of rig2＋ and CH3Hg＋in immobilized cells. We expect the probe design tactics can be generally useful for sensing many other analytes.

关键词： mercury methylmercury coordination-induced emission fluorescence enhancement selectivity

The effect of secondary structures on the generation of characteristic events during the translocation of DNA hybrid through ?-hemolysin

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Science China Chemistry 2016年第1期59卷 135-141页

作者： Xingyong Wang Yuru Li Ting Li Lei Liu hai-chen wu School of Chemical Engineering University of Mining & TechnologyXuzhou Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety Institute of High Energy Physics Chinese Academy of Sciences

Nanopore has been developed to be a powerful,single-molecule analytical tool for sensing ions,small organic molecules and biomacromolecules such as proteins and ***,the identity of the analyte can be revealed by current amplitude changes and mean dwell time of the analyte binding *** some cases,generation of highly characteristic current events affords an alternative way of analyte determination with high confidence ***,we found that secondary structures in DNA/RNA hybrids might severely hinder the generation of signature events during their translocation through?-hemolysin *** this report,we propose a strategy to add a certain concentration of urea in the buffer solution for single channel recordings and validate that low concentration of urea can effectively denature the secondary structures in DNA hybrids and recover the generation of signature *** finding might be useful in other secondary structure-related nanopore sensing activities.

关键词： nanopore sensing DNA hybrid translocation signature event urea

Significant effect of ordered micro-domain on cell boundary phase distribution and demagnetization curve squareness of Sm_(2)Co_(17)-type magnet

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Rare Metals 2024年第4期43卷 1724-1735页

作者： Zhuang Liu hai-chen wu Chao-Yue Zhang Chao-Qun Zhu Guo-Xin chen Huan-Ming Lu Ren-Jie chen Aru Yan CISRI&NIMTE Joint Innovation Center for Rare Earth Permanent Magnets Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingbo315201China University of Chinese Academy of Sciences Beijing100049China Analytical Center Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingbo315201China

Cu-rich cell boundary phase is difficult to precipitate evenly,resulting in a generally poor demagnetization curve squareness for Fe-rich Sm_(2)Co_(17)-type magnet,which is a key factor limiting the further improvement of magnetic energy *** this study,we report that nanoscale strip-like ordered micro-domains distributed in1:7H disordered matrix phase of the solid solution precursor is a new factor significantly affecting the precipitation and distribution of the cell boundary *** strip-like and continuous micro-twin structure with twin boundaries neatly perpendicular to the C-axis is observed after sintering *** solution treatment,sequential and long strip-like micro-twins gradually transform into disordered state along the basal plane,forming narrow disordered 1:7H(TbCu_(7)-type structure)phase between the separated strip-like ordered *** disordering transformation takes place via broken down of the long strip-like ordered micro-domains,which is accomplished by narrowing along the width direction followed by reduction of the ***,a new model revealing the effect of the ordered micro-domains on the formation of the cell boundary phase is *** boundaries enriched in Cu have already existed in the precursor with long strip-like ordered ***,the Cu-rich cell boundary phase acting as strong pinning centers cannot be precipitated homogeneously and distributed continuously after aging,resulting in a poor demagnetization curve squareness of Sm_(2)Co_(17)-type *** results indicate that significant broken down of the nanoscale ordered micro-domains in solution precursor is the key factor improving the distribution of cell boundary phase in Sm_(2)Co_(17)-type magnets.

关键词： Sm_(2)Co_(17) magnets Solution precursor Ordered micro-domains Disordering transformation Cellular structure

Magnetic properties and resistivity of a 2:17-type SmCo magnet doped with ZrO_(2)

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Chinese Physics B 2021年第7期30卷 551-555页

作者： Qi-Qi Yang Zhuang Liu Chao-Yue Zhang hai-chen wu Xiao-Lei Gao Yi-Long Ma Ren-Jie chen A-Ru Yan Laboratory of Rare-Earth Magnetic Functional Materials Ningbo Institute of Material Technology and EngineeringChinese Academy of SciencesNingbo 315201China College of Metallurgy and Materials Engineering Chongqing University of Science and TechnologyChongqing 401331China

In order to counteract the demagnetization caused by eddy current loss,widespread attention has been devoted to increasing the resistivity of permanent *** prepared 2:17-type Sm Co magnets doped with different ZrO_(2)contents and investigated the influence of the ZrO_(2)content on the magnetic properties and resistive *** results showed that not only was the resistivity of the magnet improved,but,in addition,the coercivity of the magnet was significantly *** microstructure was studied with TEM,which showed that ZrO_(2)doping was able to cause a decrease in the lamellar phase density and the growth of cellular *** increased grain boundaries and Sm_(2)O_(3)phases were favorable to the improvement of *** decrease of the lamellar phases caused a narrowing of the resistive *** additional Cu in the center of the cellular boundaries was the main reason for the enhancement of ***,an excessive amount caused an increase of the Zr_6(Fe Co)_(23)phase and a deterioration of the cellular structure,thereby leading to a decrease in coercivity.

关键词： 2:17-type SmCo magnets resistivity coercivity cellular structure

Atomic scale structural characterization of {10 12} twin boundaries in zinc

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Transactions of Nonferrous Metals Society of China 2018年第8期28卷 1538-1542页

作者： hai-chen wu You-xing YU Xiao-fang BI School of Materials Science and Engineering Beihang UniversityBeijing 100191China

The structure and the migration mechanisms of {10 12} twin boundaries（TBs） of pure zinc deformed by rolling were studied using high-resolution transmission electron microscopy（HRTEM） at atomic scale. We found the presence of basal/prismatic（BP/PB） planes serrations on {10 12} TBs and the coexistence of two kinds of TBs with different structures in the same {10 12} twin： TBs composed of {10 12} coherent twin boundaries（CTBs） plus short BP/PB serrations, and TBs composed of successive BP/PB segments without {10 12} CTBs. The formation of BP/PB serrations has no relation to the c/a ratio of hexagonal-closepacked（HCP） metals because the BP/PB serrations are energetically preferred and geometrically favored. Based on dislocation theory, we proposed the migration mechanisms of the TBs to be the glide of twinning dislocations（TDs） on the CTBs and the climb of interface dislocations（IDs） on the BP/PB segments.

关键词： zinc twin boundaries basal/prismatic BP/PB serrations inigration mechanism high-resolution translnission electron inicroscopy (HRTEM)

Phase structure evolution and its effect on magnetic and mechanical properties of B-doped Sm_(2)Co_(17)-type magnets with high Fe content

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Chinese Physics B 2024年第9期33卷 582-588页

作者： Yao-Wen Li Zhuang Liu hai-chen wu Fang Wang Chao-Qun Zhu Dong-Liang Tan Yu Liu Yang Yang Ming-Xiao Zhang Ren-Jie chen A-Ru Yan Key Laboratory of Magnetic Molecules and Magnetic Information Materials of Ministry ofEducation&School of Chemistry and Materials Science Shanxi Normal UniversityTaiyuan 030032China CISRI&NIMTE Joint Innovation Center for Rare Earth Permanent Magnets Ningbo Institute ofMaterial Technology and EngineeringChinese Academy of SciencesNingbo 315201China University of Chinese Academy of Sciences Beijing 100049China

The unique cellular microstructure of Fe-rich Sm_(2)Co_(17)-type permanent magnets is closely associated with the structure of the solid solution *** investigate the phase structure,magnetic properties,and mechanical behavior of B-doped Sm_(2)Co_(17)-type magnets with high Fe *** doped B atoms can diffuse into the interstitial vacancy,resulting in lattice expansion and promote the homogenization of the phase organizational structure during the solid solution treatment in ***,the resulting second phase plays a dominant role to result in more microtwin structures and highly ordered 2:17R phases in the solid solution stage,which inhibits the ordering transformation of 1:7H phase during aging and affects the generation of the cellular structure,and to result in a decrease in magnetic properties,yet the interface formed between it and the matrix phase hinders the movement of dislocations and enhances the mechanical ***,the precipitation of high flexural strain grain boundary phase induced by B element doping is also a new and effective way to improve the flexural strain of Sm_(2)Co_(17)-type *** study provides a new understanding of the phase structure evolution and its effect on the magnetic and mechanical properties of Sm_(2)Co_(17)-type magnets with high Fe content.

关键词： Sm_(2)Co_(17)-type magnets magnetic and mechanical properties

Postoperative Changes in Hemoglobin and Hematocrit in Patients Undergoing Primary Total Hip and Knee Arthroplasty

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Chinese Medical Journal 2015年第14期128卷 1977-1979页

作者： Zhong-Yi chen hai-Zhao wu Ping Zhu Xing-Bing Feng Department of Joint Surgery Taizhou Hospital Linhai Zhejiang 317000 China

INTRODUCTION Total hip arthroplasty （THA） and total knee arthroplasty （TKA） are currently two effective treatments for many diseases of the hip and knee. Despite the ability to minimize intraoperative blood loss, postoperative hemoglobin （Hgb） loss is expected, and a number of studies have reported on hidden blood loss after THA and TKA.

关键词： Hematocrit Hemoglobin Joint Replacement Postoperative Change

Reducing intimal hyperplasia in vein grafts harvested by a no-touch harvesting technique

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Journal of Pharmaceutical Analysis 2009年第2期21卷 138-140页

作者： hai-chen Wang,wu-jun Xue,Miao-miao Liu Department of Cardiovascular Surgery,the First Affiliated Hospital,Medical School of Xi’an Jiaotong University,Xi’an 710006,China. Department of Cardiovascular Surgery First Affiliated Hospital Medical School of Xi'an Jiao-tong University Xi'an 710006 China

Objective To investigate the effect of no-touch harvesting technique in reducing vein graft intimal hyperplasia. Methods This longitudinal trial compared graft angiostenosis of two groups undergoing jugular vein to carotid artery interposition grafting in rabbit model. Conventional group:12 rabbits had their veins stripped,distended,and stored in heparinized saline solution. No-touch group:12 rabbits had veins removed with surrounding tissues,but were not distended,and stored in heparinized blood. The grafts were removed 4 weeks following grafting,and morphometry and immunohistochemistry assessment were performed. Results The intimal thickness,degree of angiostenosis and proliferation index of vascular smooth muscle cells of no-touch group were significantly reduced (P<0.01) compared with those of the conventional group. The proliferating cell nuclear antigen positive-staining cells were significantly increased (P<0.01) in the conventional group compared with whose in the no-touch group. Conclusion Harvesting the vein graft with no-touch harvesting technique could significantly reduce intimal hyperplasia of the vein graft.

关键词： vein graft no-touch harvesting technique intimal hyperplasia