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Identification and significance of differential proteins in A549 cells transfected with HLCDG1

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Journal of Central South University of Technology 2005年第Z1期12卷 337-342页

作者： ZOU Fei-yan XIE hai-long ZENG Ping-yao CHEN Zhu-chu LI Feng XIAO Zhi-qiang FENG Xue-ping ZHANG Peng-fei YANG hai-yan hu wei YU Yan-hui OUYANG Yong-mei Laboratory of Cell Biology Cancer Research Institute Xiangya School of Medicine Central South University Changsha 410078 China Institute for Reproductive Immunology Research Jinan University Guangzhou 510632 China Department of Pathology of Medical College Nanhua University Hengyang 421001 China Laboratory of Cell Biology Cancer Research Institute Xiangya School of Medicine Central South University Changsha 410078 China Laboratory of Cell Biology Cancer Research Institute Xiangya School of Medicine Central South University Changsha 410078 China Medical Research Center of Xiangya Hospital Key Laboratory of Cancer Proteomics of Chinese Ministry of Health Central South University Changsha 410008 China Laboratory of Cell Biology Cancer Research Institute Xiangya School of Medicine Central South University Changsha 410078 China Medical Research Center of Xiangya Hospital Key Laboratory of Cancer Proteomics of Chinese Ministry of Health Central South University Changsha 410008 China Medical Research Center of Xiangya Hospital Key Laboratory of Cancer Proteomics of Chinese Ministry of Health Central South University Changsha 410008 China Medical Research Center of Xiangya Hospital Key Laboratory of Cancer Proteomics of Chinese Ministry of Health Central South University Changsha 410008 China Laboratory of Cell Biology Cancer Research Institute Xiangya School of Medicine Central South University Changsha 410078 China Medical Research Center of Xiangya Hospital Key Laboratory of Cancer Proteomics of Chinese Ministry of Health Central South University Changsha 410008 China Laboratory of Cell Biology Cancer Research Institute Xiangya School of Medicine Central South University Changsha 410078 China Medical Research Center of Xiangya Hospital Key Laboratory of Cancer Proteomics of Chinese Ministry of Health Central South University Changsha 410008 China Laboratory of Cell Biology Cancer Research Institute Xiangya School of Medicine Central South University Chang

HLCDG1, which locates in chromosome 5q33, is a novel gene cloned recently. The HLCDG1 expression was significantly down regulated in the primary lung carcinoma. It was previously studied that HLCDG1 acted like a tumor suppressor gene. In this paper, proteomics studies were performed to analyze the proteomic expression patterns in the HLCDG1-transfected human lung carcinoma cell line (A549-HLCDG1) and in the control vector-transfecred human lung carcinoma cell line (A549-vector). Employing two dimensional gel eleetrophoresis (2DE), the global pattern of protein expressions in A549-HLCDG1 human lung adenocarcinoma cell line expressing stably HL-CDG1 gene were compared with those of control A549-vector cell line to generate a differential protein expression catalog. Forty-two differentially expressed proteins were screened. Thirteen differential proteins were identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS), which were 6 upregulated (MSH5, MOD, MDH precursor, ETFβ, Prxd Ⅵ and JM23) and 7 downregulated (PLC-δ1, hnRNPA2,hnRNPB1, TIM, TCTP, nm23H-1 and PrxdⅤ) proteins in A549-HLCDG1 cells compared to control A549-vector cells. The above identified proteins were involved in energy metabolism, transcription regulation, antioxidation,cell cycle, metastasis, DNA methylation and mismatch repair. Therefore, these differential expression proteins by HLCDG1 transfection may play some important roles for investigation of the biochemical basis of growth suppression of HLCDG1 gene in lung carcinoma cells A549. Further understanding of this data base may provide valuable resources for the developing novel diagnostic markers and therapeutic targets of lung cancer.

关键词： HLCDG1 gene lung carcinoma cell two-dimensional gel electrophoresis mass spectrometry proteomics

Fabrication and Mechano-sensing Characteristics of Bending Polypyrrole Actuator

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 240-245页

作者： CHEN Jinyou hu wei School of Automotive Engineering Hunan Financial&Industrial Vocational-technical CollegeHengyang 421002China College of Mechanical and Electrical Engineering Hunan Agricultural UniversityChangsha 410128China

To prepare a conductive polymer actuator with decent performance,a self-built experimental platform for the preparation of polypyrrole film is *** of the essential goals is to examine the mechanical characteristics of the actuator in the presence of various combinations of process parameters,combined with the orthogonal test method of"four factors and three levels".The bending and sensing characteristics of actuators of various sizes are methodically examined using a self-made bending polypyrrole *** functional relationship between the bending displacement and the output voltage signal is established by studying the characteristics of the actuator sensor subjected to various degrees of *** experimental results reveal that the bending displacement of the actuator tip almost exhibits a linear variation as a function of length and *** the voltage reaches 0.8 V,the bending speed of the actuator tends to be ***,the mechanical properties of the self-assembled polypyrrole actuator are verified by the design and fabrication of the microgripper.

关键词： conductive polymer polypyrrole mechanical characteristics actuators sensing characteristics

Splenectomy protects experimental rats from cerebral damage after stroke due to anti-inflammatory effects

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Chinese Medical Journal 2013年第12期126卷 2354-2360页

作者： ZHANG Bing-jun MEN Xue-jiao LU Zheng-qi LI hai-yan QIU wei hu Xue-qiang Department of Neurology Third Affiliated Hospital of Sun Yat-sen University Guangzhou Guangdong 510630 China

Background A recent study demonstrated that the inflammatory response accompanying necrotic brain injury played an important role in ***,inhibition of this response may help to stop the expansion of *** has been also shown that the spleen,a major peripheral immune organ,plays a role in stroke-induced immune *** study aimed to establish rat models of middle cerebral artery occlusion （MCAO） and to investigate the effect of splenectomy and possible mechanisms in that rat *** Infarct size in a stroke model was measured with the Nissl body staining method,numbers of inflammatory cells in ischemic regions were detected by immunofluorescence staining,and inflammatory factors were assayed by enzyme-linked immunosorbent assay and real-time polymerase chain reaction （PCR） in brain homogenates and *** significance of differences was determined by one-way analysis of variance （ANOVA） followed by the least significant difference post hoc *** Infarct size in the brain of rats that underwent splenectomies 2 weeks before permanent MCAO （（34.93±3.23）%） was over 50% smaller than that of rats subjected to the stroke surgery alone （（74.33±2.36）%,P 〈0.001; （77.30±2.62）%,P 〈0.001）.Lower numbers of T cells,neutrophils,and macrophages in brain tissue and lower levels of pro-inflammatory cytokines,such as interleukin （IL）-1β and tumor necrosis factor （TNF）-α,were observed in rats that underwent splenectomies,compared with the two other groups,but splenectomized rats showed higher levels of the anti-inflammatory factor IL-10 in the *** The mechanism（s） by which splenectomy protects brain from damage induced by stroke may correlate with the decreased numbers of inflammatory cells and changes in inflammatory cytokines.

关键词： cerebral ischemia splenectomy immune system inflammation cells cytokines

Eukaryotic expression and biological activity analysis of neuroprotective peptide [Gly14]-humanin

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Journal of Pharmaceutical Analysis 2010年第2期22卷 105-110页

作者： hui Jin,hai-Tao hu ,wei-Xi Wang,Gai-Feng Feng,Zhao-hui Liu,wei-Na YangDepartment of human Anatomy and Histoembryology,Medical School of Xi’an Jiaotong University,Xi’an 710061,China. Department of human Anatomy and Histoembryology Medical School of Xi'an Jiaotong University Xi'an 710061 China

Objective To investigate the expression of neuroprotective peptide [Gly14]-humanin (HNG) in eukaryotic cells by gene engineering technique and analyze its biological activity. Methods By means of asymmetrical primer/template,double stranded cDNA of HNG with FLAG in its C-terminal was obtained,which was cloned into the plasmid pcDNA3.1(-),and the resultant recombinant vector pcDNA3.1(-)/HNG-FLAG was transfected into PC12 cells. At the same time,the recombinant vector pcDNA3.1(-)/EGFP was transfected to control the efficiency of transfection. The expression of HNG in the cells was determined by immunocytochemistry. In order to analyze the biological activity of the expressed HNG,25μM Aβ25-35 peptide was added to the culture medium of the transfected cells for 24h,then cell morphology,MTT assay and Hoechst 33258 staining were observed. Results The eukaryotic expression vector of pcDNA3.1(-)/HNG-FLAG was identified by enzyme digestion and sequencing. HNG was highly expressed in PC12 cells. After exposure of PC12 cells to 25μM Aβ25-35 for 24h,cell viability decreased to (65.8±5.3)%,and the dystrophic changes of neuritis and nuclei condensation were obvious. When cells were pre-transfected with pcDNA3.1(-)/HNG-FLAG,Aβ25-35-induced cell death and morphological changes of cells and nuclei were suppressed. In contrast,pre-transfected with empty vector did not protect cells from Aβ25-35-induced toxicity. Conclusion The eukaryotic expression vector for FLAG-tagged HNG was successfully constructed and expressed in PC12 cells. Expressed HNG has biological activity.

关键词： Alzheimer's disease [Gly14]-humanin eukaryotic expression biological activity

Boosting Oxygen Evolution Reaction Performance on NiFe‑Based Catalysts Through d‑Orbital Hybridization

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Nano-Micro Letters 2025年第1期17卷 281-292页

作者： Xing Wang wei Pi Sheng hu haifeng Bao Na Yao wei Luo State Key Laboratory of New Textile Materials and Advanced Processing Technology Key Laboratory of New Textile Materials and Applications of Hubei ProvinceSchool of Materials Science and EngineeringWuhan Textile UniversityWuhan 430200People’s Republic of China College of Chemistry and Molecular Sciences Wuhan UniversityWuhan 430072People’s Republic of China

Anion-exchange membrane water electrolyzers(AEMWEs)for green hydrogen production have received intensive attention due to their feasibility of using earth-abundant NiFe-based *** introducing a third metal into NiFe-based catalysts to construct asymmetrical M-NiFe units,the d-orbital and electronic structures can be adjusted,which is an important strategy to achieve sufficient oxygen evolution reaction(OER)performance in ***,the ternary NiFeM(M:La,Mo)catalysts featured with distinct M-NiFe units and varying d-orbitals are reported in this *** and theoretical calculation results reveal that the doping of La leads to optimized hybridization between d orbital in NiFeM and 2p in oxygen,resulting in enhanced adsorption strength of oxygen intermediates,and reduced rate-determining step energy barrier,which is responsible for the enhanced OER *** critically,the obtained NiFeLa catalyst only requires 1.58 V to reach 1 A cm^(−2) in an anion exchange membrane electrolyzer and demonstrates excellent long-term stability of up to 600 h.

关键词： NiFe-based catalysts d-orbital coupling Oxygen evolution reaction Anion exchange membrane electrolyzer

Mast cell- derived mediators protects PC12 cells against ischemia in vitro

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中国药理通讯 2006年第4期23卷 37-38页

作者： Fan Yan-Yi Shen Yao Fu Qiu-Li Dai hai-Bin hu wei-wei Chen Zhong Department of Pharmacology School of Medicine Zhejiang University Hangzhou China 310058

Monitoring immune function after rapid corticosteroid reduction in kidney transplant recipients

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Chinese Medical Journal 2011年第5期124卷 679-682页

作者： LI Shi-hai WANG wei hu Xiao-peng YIN Hang REN Liang YANG Xiao-yong LIU Hang ZHANG Xiao-dong Department of Urology Beijing Chaoyang Hospital of Capital Medical University Beijing 100020 China

Background Long-term use of steroid with large dosage might cause many adverse effects in kidney transplant patients; reducing steroid dosage to a low level for maintenance is helpful in avoiding the side-effects, but meanwhile, acute rejection may rise to be a main concern. The present research monitored the immune function changes and the incidence of acute rejection and infection after rapid steroid reduction to investiclate the safety of this *** A prospective trial was conducted, using tacrolimus and mycophenolate mofetil as the basic immunosuppressive regimen, in addition to antibody induction with basiliximab. Corticosteroid dosage was rapidly reduced to 10 mg/d seven days post-transplantation in the experimental group, and the standard corticosteroid therapy was employed in the control group. Patient immunity was monitored by the Immune Cell Function Assay pre- and two weeks post-transplantation. The incidence of acute rejection and infection were compared between the experimental and control *** Comparison of intracellular adenosine triphosphate （iATP） values detected two weeks post-transplantation for the control group （（324±45） ng/ml） and the experimental group （（345±91） ng/ml） did not reveal a significant difference （P 〉0.05）. The incidence of acute rejection was analogous between groups （P 〉0.05）, while an increased incidence of infection was observed in the control group （53% （n=-16）） versus the experimental group （22% （n=-6）, P 〈0.05）. Overall, recipients in the control group had longer and more recurrent infections than those in the experimental group （P 〈0.05）. Patients in the control group had a lower immune response （（235±35） ng/ml） than those in the experimental group （（286±16） ng/ml） when infection occurred （P 〈0.05）.Conclusion Rapid reduction of steroid early after kidney transplantation does not lead to a significant rise in patient immunity. It is a safe and effective therapy for kidney transplant patients.

关键词： kidney transplantation corticosteroid reduction acute rejection immune cell function assay infection

A functional cathode sodium compensation agent for stable sodium-ion batteries

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Green Energy & Environment 2025年第1期10卷 173-182页

作者： wei Wu Zhenglin hu Zhengfei Zhao Aoxuan Wang Jiayan Luo Key Laboratory for Green Chemical Technology of Ministry of Education School of Chemical Engineering and TechnologyTianjin UniversityTianjin300072China State Key Laboratory of Metal Matrix Composites School of Materials Science and EngineeringShanghai Jiao Tong UniversityShanghai200240China Shanghai Jiao Tong University Shaoxing Research Institute of Renewable Energy and Molecular Engineering Shaoxing312000China

Hard carbon(HC)is widely used in sodium-ion batteries(SIBs),but its performance has always been limited by lowinitial Coulombic efficiency(ICE)and cycling *** compensation agent is a favorable strategy to make up for the loss of active sodium ions consumed *** it lacks agent that effectively decomposes to increase the active sodium ions as well as regulate carbon defects for decreasing the irreversible sodium ions ***,we propose 1,2-dihydroxybenzene Na salt(NaDB)as a cathode compensation agent with high specific capacity(347.9 mAh g^(-1)),lower desodiation potential(2.4–2.8 V)and high utilization(99%).Meanwhile,its byproduct could functionalize HC with more C]O groups and promote its reversible ***,the presodiation hard carbon(pHC)anode exhibits highly reversible capacity of 204.7 mAh g^(-1) with 98%retention at 5 C rate over 1000 ***,with 5 wt%NaDB initially coated on the Na3V2(PO4)3(NVP)cathode,the capacity retention of NVP t NaDBjHC cell could increase from 22%to 89%after 1000 cycles at 1 C *** work provides a new avenue to improve reversible capacity and cycling performance of SIBs through designing functional cathode compensation agent.

关键词： Hard carbon ICE Cathode compensation agent Reversible capacity Stability

Domain generalization with semi-supervised learning for people-centric activity recognition

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Science China(Information Sciences) 2025年第1期68卷 171-188页

作者： Jing LIU wei Zhu Di LI Xing hu Liang SONG Academy for Engineering & Technology Fudan University Shanghai East-bund Research Institute on Networking Systems of AI School of Optoelectronic Information and Computer Engineering University of Shanghai for Science & Technology

People-centric activity recognition is one of the most critical technologies in a wide range of real-world applications,including intelligent transportation systems, healthcare services, and brain-computer interfaces. Large-scale data collection and annotation make the application of machine learning algorithms prohibitively expensive when adapting to new tasks. One way of circumventing this limitation is to train the model in a semi-supervised learning manner that utilizes a percentage of unlabeled data to reduce the labeling burden in prediction tasks. Despite their appeal, these models often assume that labeled and unlabeled data come from similar distributions, which leads to the domain shift problem caused by the presence of distribution gaps. To address these limitations, we propose herein a novel method for people-centric activity recognition,called domain generalization with semi-supervised learning(DGSSL), that effectively enhances the representation learning and domain alignment capabilities of a model. We first design a new autoregressive discriminator for adversarial training between unlabeled and labeled source domains, extracting domain-specific features to reduce the distribution gaps. Second, we introduce two reconstruction tasks to capture the task-specific features to avoid losing information related to representation learning while maintaining task-specific consistency. Finally, benefiting from the collaborative optimization of these two tasks, the model can accurately predict both the domain and category labels of the source domains for the classification task. We conduct extensive experiments on three real-world sensing datasets. The experimental results show that DGSSL surpasses the three state-of-the-art methods with better performance and generalization.

关键词： activity recognition deep learning domain generalization semi-supervised learning adversarial training

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