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Rice science 2024年第2期31卷 190-202,I0022-I0028页

作者： WU Lijuan HAN Cong WANG Huimei he Yuchang LIN hai WANG Lei CheN Chen E Zhiguo State Key Laboratory of Rice Biology and Breeding China National Rice Research InstituteHangzhou 310006China Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Agricultural College of Yangzhou UniversityYangzhou 225009China

The basic region/leucine zipper(bZIP)transcription factors play important roles in plant development and responses to abiotic and biotic ***53 regulates resistance to Magnaporthe oryzae in rice by analyzing APIP5-RNAi transgenic *** further investigate the biological functions of OsbZIP53,we generated osbzip53 mutants using CRISPR/Cas9 editing and also constructed OsbZIP53 over-expression transgenic *** analysis of phenotypical,physiological,and transcriptional data showed that knocking-out OsbZIP53 not only improved disease resistance by inducing a hypersensitivity response in plants,but also regulated the immune response through the salicylic acid ***,disrupting OsbZIP53 increased H2O2 accumulation by promoting reactive oxygen species generation through up-regulation of several respiratory burst oxidase homologs(Osrboh genes)and weakened H2O2 degradation by directly targeting *** addition,the growth of osbzip53 mutants was seriously impaired,while OsbZIP53 over-expression lines displayed a similar phenotype to the wild type,suggesting that OsbZIP53 has a balancing effect on rice immune response and growth.

关键词： OsbZIP53 hypersensitive response reactive oxygen species metabolism rice immunity salicylic acid transcription factor

Theoretical Study on the Intermolecular Interactions of Tetrazole Dimers

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Chinese Journal of Structural Chemistry 2005年第10期24卷 1203-1210页

作者： ZHOU Su-Qin JU Xue-hai XIAO he-Ming Department of Chemistry Nanjing University of Science and Technology Nanjing 210094 China

Tetrazole monomers （Ⅰ, Ⅱ） and all of their possible stable dimers （1, 2, 3, 4, 5, 6, 7 and 8） were fully optimized by DFT method at the B3LYP/6-311＋＋G^＊＊ level. Among the eight dimers, there were two 1H-tetrazole dimers, three 2H-tetrazole dimers and three hetero dimers of 1H-tetrazole and 2H-tetrazole. Vibrational frequencies were calculated to ascertain that each structure was stable （no imaginary frequencies）. The basis set superposition errors （BSSE） are 2.78, 2.28, 2.97, 2.75, 2.74, 2.18, 1.23 and 3.10 kJ/mol, and the zero point energy （ZPE） corrections for the interaction energies are 4.88, 4.18, 3.87, 3.65, 3.54, 3.22, 2.87 and 4.34 kJ/mol for 1, 2, 3, 4, 5, 6, 7 and 8, respectively. After BSSE and ZPE corrections, the greatest corrected intermolecular interaction energy of the dimers is -43.71 kJ/mol. The charge redistribution mainly occurs on the adjacent N-H…N atoms between submolecules. The charge transfer between two subsystems is very small. Natural bond orbital （NBO） analysis was performed to reveal the origin of the interaction. Based on the statistical thermodynamic method, the standard thermodynamic functions, heat capacities （C^0P）, entropies （S^0T） and thermal corrections to enthalpy （H^0T）, and the changes of thermodynamic properties from monomer to dimer in the temperature range of 200.00 K to 700 K have been obtained. 1H-tetrazole monomer can spontaneously turn into two stable dimers at 298.15 K.

关键词： tetrazole dimer intermolecular interaction DFT natural bond orbital,thermodynamic properties

Air-Ground Collaborative Mobile Edge Computing:Architecture,Challenges,and Opportunities

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China Communications 2024年第5期21卷 1-16页

作者： Qin Zhen he Shoushuai Wang hai Qu Yuben Dai haipeng Xiong Fei Wei Zhenhua Li hailong College of Communications Engineering Army Engineering University of PLANanjing 210000China Department of Information and Communication Noncommissioned Officer Academy of PAPHangzhou 310000China Key Laboratory of Dynamic Cognitive System of Electromagnetic Spectrum Space Ministry of Industry and Information TechnologyNanjing 211106China College of Electronic and Information Engineering Nanjing University of Aeronautics and AstronauticsNanjing 211106China State Key Laboratory for Novel Software Technology Nanjing UniversityNanjing 210023China CMC Political and Law Commission Beijing100120China Xi’an Research Institute of High Technology Xi’an 710025China

By pushing computation,cache,and network control to the edge,mobile edge computing(MEC)is expected to play a leading role in fifth generation(5G)and future sixth generation(6G).Nevertheless,facing ubiquitous fast-growing computational demands,it is impossible for a single MEC paradigm to effectively support high-quality intelligent services at end user equipments(UEs).To address this issue,we propose an air-ground collaborative MEC(AGCMEC)architecture in this *** proposed AGCMEC integrates all potentially available MEC servers within air and ground in the envisioned 6G,by a variety of collaborative ways to provide computation services at their best for ***,we introduce the AGC-MEC architecture and elaborate three typical use ***,we discuss four main challenges in the AGC-MEC as well as their potential ***,we conduct a case study of collaborative service placement for AGC-MEC to validate the effectiveness of the proposed collaborative service placement ***,we highlight several potential research directions of the AGC-MEC.

关键词： air-ground architecture collaborative mobile edge computing

Experimental study on piping in sandy gravel foundations considering effect of overlying clay

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Water Science and Engineering 2016年第2期9卷 165-171页

作者： Shuang Wang Jian-sheng Chen hai-qing he Wen-zheng he College of Civil and Transportation Engineering Hohai University College of Earth Sciences and Engineering Hohai University

The influence of the overlying clay on the progression of piping in the sandy gravel foundation of water-retaining structures is often neglected. In order to study this influence, an experimental investigation was conducted on a laboratory-scale model. It was discovered that the critical hydraulic gradient and the area of the piping tunnel increase when the overlying clay thickens. With a thicker clay layer, erosion of the sandy gravel below the clay layer occurs later, but, once the erosion starts, the erosion rate is very high and the average velocity of water seeping through the cross-section of the sandy gravel increases rapidly due to the low deformability of the thick clay layer. Furthermore, it was found that the progression of piping is a complicated and iterative process involving erosion of fine particles, clogging of pores, and flushing of the clogged pores. Two types of erosion have been identified in the progression of piping: one causes the tunnel to advance upstream, and the other increases the depth of the tunnel. The results show that the overlying clay is an important factor when evaluating piping in sandy gravel foundations of water-retaining structures.

关键词： Piping Experimental model Sandy gravel foundation Overlying clay Clog Erosion rate

Manipulating the Phase Transition Behavior of Dual Temperature-Responsive Block Copolymers by Adjusting Composition and Sequence

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Chinese Journal of Polymer Science 2024年第2期42卷 176-187,I0006页

作者： Zhi Zou Xiang Xu hai-Tao Zhao Jian-Nan Cheng Wei-Wei he Li-Fen Zhang Zhen-Ping Cheng Suzhou key Laboratory of Macromolecular Design and Precision Synthesis Jiangsu Key Laboratory of Advanced Functional Polymer Design and ApplicationState and Local Joint Engineering Laboratory for Novel Functional Polymeric MaterialsCollege of ChemistryChemical Engineering and Materials ScienceSoochow UniversitySuzhou215123China State Key Laboratory of Radiation Medicine and Protection School for Radiological and Interdisciplinary Sciences(RAD-X)and Collaborative Innovation Center of Radiation Medicine of JiangsuSoochow UniversitySuzhou215123China

Temperature-responsive polymers have garnered significant attention due to their ability to respond to external *** this work,dual temperature-responsive block copolymers are synthesized via reversible addition-fragmentation chain transfer polymerization(RAFT)polymerization utilizing zwitterionic monomer methacryloyl ethyl sulfobetaine(SBMA) and N-isopropyl acrylamide(NIPAAm) as *** thermal responsive behaviors can be easily modulated by incorporating additional hydrophobic monomer benzyl acrylate(BN) or hydrophilic monomer acrylic acid(AA),adjusting concentration or pH,or varying the degree of polymerization of the block chain *** cloud points of the copolymers are determined by UV-Vis spectrophotometry,and these copolymers exhibit both controlled upper and lower critical solu bility temperatures(LCST and UCST) in aqueous *** study analyzes and summarizes the influencing factors of dual temperature responsive block copolymers by exploring the effects of various conditions on the phase transition temperature of temperature-sensitive polymers to explore the relationship between their properties and environment and structure to make them more selective in terms of temperature application range and regulation *** is very interesting that the introduction of poly-acrylic acid(PAA) segments in the middle of di-block copolymer PSBMA_(55)-b-PNIPAAm_(80) to form PSBMA_(55)-b-PAA_(x)-b-PNIPAAm_(80) results in a reversal of temperature-responsive behaviors from 'U'(LCST UCST) type,while the copolymer PSBMA_(55)-b-P(NIPAAm_(80)-co-AA_(x)) *** work provides a clue for tuning the phase transition behavior of polymers for manufacture of extreme smart materials.

关键词： Temperature responsive polymers High critical solubility temperature(UCST) Low critical solubility temperature(LCST) Block copolymers

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控制玉米株高基因PHR1的基因克隆

作物学报 2024年第1期50卷 55-66页

作者：杨晨曦周文期周香艳刘忠祥周玉乾刘芥杉杨彦忠何海军王晓娟连晓荣李永生甘肃农业大学生命科学技术学院甘肃兰州730070 甘肃省农业科学院作物研究所甘肃兰州730070

株高属于玉米理想株型育种的一个重要指标,不但影响玉米机械化收获,更与玉米的倒伏性和生物产量密切相关。本研究以低剂量快中子(4.19 Gy)辐照诱变玉米自交系KWS39获得的矮秆低穗位突变体为研究对象,该突变体命名为plant height reducin... 详细信息

株高属于玉米理想株型育种的一个重要指标,不但影响玉米机械化收获,更与玉米的倒伏性和生物产量密切相关。本研究以低剂量快中子(4.19 Gy)辐照诱变玉米自交系KWS39获得的矮秆低穗位突变体为研究对象,该突变体命名为plant height reducing mutant-1(phr-1),开展了表型性状的田间调查分析,并利用phr-1×B73获得的F2分离群体,借助极端性状混池测序分析法(BSA-seq)及目标区段重组交换鉴定的方法,基于B73参考基因组对目标区段内的基因进行挖掘和功能注释,定位候选基因。研究结果表明,在1号染色体Bin1.06区间可能存在变异位点,进而利用大的分离群体结合目标区段多态性标记开发,将目标区段精细定位分子标记到Umc1122和Umc1583a两个标记之间约600 kb区间,该区段内存在一个控制株高的已知基因Brachytic2(BR2),BR2编码一个调控玉米茎秆中生长素极性运输的糖蛋白。候选基因测序结果表明,phr-1是BR2基因在第4个外显子处插入了165 bp的序列,导致第547位氨基酸变为终止子,蛋白翻译提前终止。phr-1的基因突变位点和变异方式与已报道的br2-1单个碱基发生变异位点完全不同,通过等位杂交实验证明了phr-1突变体就是br2-1的一个新等位突变体,候选基因就是BR2基因。本研究为玉米BR2基因在玉米株高遗传改良中提供了新的种质资源。

关键词：玉米株高穗位高 BSA混池测序基因定位功能分析

Carbides Evolution in 12Cr Martensitic heat-resistant Steel with Life Depletion for Long-term Service

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Journal of Iron and Steel Research(International) 2015年第3期22卷 250-255页

作者： Zheng-fei HU Da-hai he Fan MO School of Materials Science and Engineering Tongji University

Evaluating the residual life of exposed components in power industry is a very important procedure inroutine examination. The microstructures of a series of X20CrMoV12.1 martensitic superheater tube samples in a boiler in different service periods were investigated extensively to extract a quantitative relationship. During long-term service from start to rupture, hardness decreased monotonically with life depletion, and the decrease of hardness in prior austenite grain boundary was steeper than that in the matrix. Microstructure observation showed obvious damage characteristics, including carbide coarsening and martensite decomposing, and the martensite structure decomposed completely in rupture state. The morphology, distribution and composition of the main precipitates M23 C6 varied distinctly. The aspect ratio of coarsened carbides along grain boundary increased several fold with respect to their original size. The composition of coarsened M23 C6 carbide shows the most regular trend of Cr enrichment and the statistical result of Cr enrichment in M23 C6 shows a linear correlation between the ratio of Cr to Fe and service time to the power of 3/2, which may be considered as an index of material degradation due to long-term service exposure.

关键词： 12Cr martensitic heat-resistant steel carbide microstructure creep damage element enrichment longterm service

Working condition recognition of sucker rod pumping system based on 4-segment time-frequency signature matrix and deep learning

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Petroleum Science 2024年第1期21卷 641-653页

作者： Yun-Peng he hai-Bo Cheng Peng Zeng Chuan-Zhi Zang Qing-Wei Dong Guang-Xi Wan Xiao-Ting Dong State Key Laboratory of Robotics Shenyang Institute of AutomationChinese Academy of SciencesShenyang 110016LiaoningChina Key Laboratory of Networked Control Systems Chinese Academy of SciencesShenyang 110016LiaoningChina Institutes for Robotics and Intelligent Manufacturing Chinese Academy of SciencesShenyang 110169LiaoningChina University of Chinese Academy of Sciences Beijing100049China School of Artifcial Intelligence Shenyang University of TechnologyShenyang 110870LiaoningChina

High-precision and real-time diagnosis of sucker rod pumping system(SRPS)is important for quickly mastering oil well *** learning-based method for classifying the dynamometer card(DC)of oil wells is an efficient diagnosis ***,the input of the DC as a two-dimensional image into the deep learning framework suffers from low feature utilization and high computational ***,different SRPSs in an oil field have various system parameters,and the same SRPS generates different DCs at different ***,there is heterogeneity in field data,which can dramatically impair the diagnostic *** solve the above problems,a working condition recognition method based on 4-segment time-frequency signature matrix(4S-TFSM)and deep learning is presented in this ***,the 4-segment time-frequency signature(4S-TFS)method that can reduce the computing power requirements is proposed for feature extraction of DC ***,the 4S-TFSM is constructed by relative normalization and matrix calculation to synthesize the features of multiple data and solve the problem of data ***,a convolutional neural network(CNN),one of the deep learning frameworks,is used to determine the functioning conditions based on the *** on field data verify that the proposed diagnostic method based on 4S-TFSM and CNN(4S-TFSM-CNN)can significantly improve the accuracy of working condition recognition with lower computational *** the best of our knowledge,this is the first work to discuss the effect of data heterogeneity on the working condition recognition performance of SRPS.

关键词： Sucker-rod pumping system Dynamometer card Working condition recognition Deep learning Time-frequency signature Time-frequency signature matrix

Digital generation of super-Gaussian perfect vortex beams via wavefront shaping with globally adaptive feedback

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High Power Laser Science and Engineering 2024年第1期12卷 42-51页

作者： Rui Ma Ke hai Luo Jing Song he Wei Li Zhang Dian Yuan Fan Anderson S.L.Gomes Jun Liu International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education Institute of Microscale OptoelectronicsShenzhen UniversityShenzhenChina Institute for Advanced Study Shenzhen UniversityShenzhenChina Fiber Optics Research Centre School of Information and Communication EngineeringUniversity of Electronic Science and Technology of ChinaChengduChina Departamento de Física Universidade Federal de PernambucoRecife-PEBrazil

High-intensity vortex beams with tunable topological charges and low coherence are highly demanded in applications such as inertial confinement fusion(ICF) and optical communication. However, traditional optical vortices featuring nonuniform intensity distributions are dramatically restricted in application scenarios that require a high-intensity vortex beam owing to their ineffective amplification resulting from the intensity-dependent nonlinear effect. here, a low-coherence perfect vortex beam(PVB) with a topological charge as high as 140 is realized based on the super-pixel wavefront-shaping technique. More importantly, a globally adaptive feedback algorithm(GAFA) is proposed to efficiently suppress the original intensity fluctuation and achieve a flat-top PVB with dramatically reduced beam speckle contrast. The GAFA-based flat-top PVB generation method can pave the way for high-intensity vortex beam generation,which is crucial for potential applications in ICF, laser processing, optical communication and optical trapping.

关键词： digital micromirror device flat-top beam orbital angular momentum perfect vortex beam random fiber laser

玉米碳水化合物分配缺陷突变体ygl3的光合特性及遗传分析

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玉米科学 2024年第3期32卷 32-41页

作者：刘忠祥李永生王晓娟连晓荣梁根生寇向龙何海军杨彦忠周文期周玉乾甘肃省农业科学院作物研究所兰州730070 甘肃省农业科学院农业质量标准与检测技术研究所兰州730070

以快中子辐射诱变玉米自交系PH6WC筛选得到的碳水化合物分配缺陷突变体ygl3为材料,通过叶片内叶绿素含量和碳水化合物含量的测定、叶绿体结构显微观察、光合特性和遗传特性等进行分析。结果表明,与野生型相比,突变体叶绿体数目变少,形... 详细信息

以快中子辐射诱变玉米自交系PH6WC筛选得到的碳水化合物分配缺陷突变体ygl3为材料,通过叶片内叶绿素含量和碳水化合物含量的测定、叶绿体结构显微观察、光合特性和遗传特性等进行分析。结果表明,与野生型相比,突变体叶绿体数目变少,形态松散,维管束鞘叶绿体积累了大量的淀粉,淀粉颗粒排列不规则且体积增大,导致了叶绿体的膨胀和膜结构的破坏;叶片中淀粉、葡萄糖、果糖的含量都显著高于野生型,表现出玉米碳水化合物分配缺陷的典型表型特征;突变体的叶绿素a、叶绿素b及总叶绿素含量都显著低于野生型;净光合速率(Photo)、气孔导度(Cond)、蒸腾速率(Trmmol)均极显著低于野生型,胞间二氧化碳浓度(Ci)高于野生型。遗传分析表明,(ygl3×Mo17)F_(2)分离群体突变型(黄化)与野生型(绿色)植株性状分离符合1∶3分离比例,表明该突变体受单个核隐性基因控制。

关键词：玉米碳水化合物突变体ygl3 光合特性遗传分析