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Cloning and characterization of a novel UDP-glycosyltransferase gene induced by DON from wheat

Journal of Integrative Agriculture 2015年第5期14卷 830-838页

作者： MA Xin DU Xu-ye LIU Guo-juan YANG Zai-dong hou wen-qian WANG Hong-wei FENG De-shun LI An-fei KONG Ling-rang State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology College of AgronomyShandong Agricultural University

Fusarium head blight（FHB）,caused primarily by Fusarium graminearum,is a destructive disease of wheat throughout the ***,the mechanisms of host resistance to FHB are still largely ***（DON）produced by F graminearum which enhances the pathogen to spread could be converted into inactive form D3 G by UDP-glycosyltransferases（UGTs）.A DON responsive UGT gene,designated as TaUGT4,was first cloned from wheat in this *** putative open reading frame（ORF） of TaUGT4 was 1386 bp,encoding 461 amino acids ***4 was placed on chromosome 2D using a set of nulli-tetrasomic lines of wheat cultivar Chinese Spring（CS）.When fused with eGFP at C terminal,TaUGT4 was shown to localize in cytoplasm of the transformed tobacco *** transcriptional analysis revealed that TaUGT4 was strongly induced by F graminearum or DON in both of FHB-resistant cultivar Sumai 3 and susceptible cultivar Kenong 199,especially in Sumai 3 under DON *** increase of TaUGT4 expression was observed in Sumai 3 and Kenong 199 in response to salicylic acid（SA） *** interestingly,the transcripts level of TaUGT4 in Sumai 3 showed significantly higher than that in Kenong 199 after treated with methyl jasmonate（MeJA）.According to the expression patterns,TaUGT4 might lead to different effects between FHB-resistant genotype and susceptible genotype in the process against F graminearum *** had also been discussed in this paper that JA signaling pathway might play a significant role in the resistance against *** compared to SA signaling pathway.

关键词： wheat cultivar chromosome designated genotype blight tobacco responsive susceptible pathogen

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Functional analysis of a wheat pleiotropic drug resistance gene involved in Fusarium head blight resistance

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Journal of Integrative Agriculture 2016年第10期15卷 2215-2227页

作者： WANG Gui-ping hou wen-qian ZHANG Lei WU Hong-yan ZHAO Lan-fei DU Xu-ye MA Xin LI An-fei WANG Hong-wei KONG Ling-rang State Key Laboratory of Crop Biology/College of Agronomy Shandong Agricultural University College of Life Science Guizhou Normal University College of Horticulture and Forestry Sciences Huazhong Agricultural University

The pleiotropic drug resistance（PDR） sub-family of adenosine triphosphate（ATP）-binding cassette（ABC） transporter had been reported to participate in diverse biological processes of plant. In this study, we cloned three novel PDR genes in Fusarium head blight（FHB） resistant wheat cultivar Ning 7840, which were located on wheat chromosomes 6A, 6B and 6D. In phylogeny, these genes were members of cluster I together with Ae PDR7 and Bd PDR7. Subcellular localization analysis showed that Ta PDR7 was expressed on the plasmalemma. The quantitative real time PCR（RT-PCR） analysis showed that this gene and its probable orthologues in chromosomes 6B and 6D were both up-regulated sharply at 48 h after infected by Fusarium graminearum and trichothecene deoxynivalenol（DON） in spike. When knocking down the transcripts of all Ta PDR7 members by barely stripe mosaic virus-induced gene silencing（BSMV-VIGS） system, it could promote the F. graminearum hyphae growth and made larger pathogen inoculation points in Ning 7840, which suggested that Ta PDR7 might play an important role in response to F. graminearum. Although salicylic acid（SA）, methyl jasmonate（Me JA） and abscisic acid（ABA） had been reported to possibly regulate wheat FHB resistance, here, we found that the three members of Ta PDR7 were negatively regulated by these three hormones but positively regulated by indoleacetic acid（IAA）.

关键词： PDR FHB VIGS

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Effects of antibacterial compounds produced by Saccharomyces cerevisiae in Koumiss on pathogenic Escherichia coli O8 and its cell surface characteristics

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Journal of Integrative Agriculture 2017年第3期16卷 742-748页

作者： CHEN Yu-jie WANG Chun-jie hou wen-qian WANG Xiao-shuo GALI Bing-ga HUASAI Si-mu-jide YANG Si-qin WU A-qi-ma ZHAO Yu-fei WU Ying-ga CHEN Ao-ri-ge-le Vocational and Technical College Inner Mongolia Agricultural University Baotou 014109 P.R. China College of Animal Science Inner Mongolia Agricultural university Hohhot 010018 P.R. China College of Veterinary Medicine Inner Mongolia Agricultural University Hohhot 010018 P.R. China

The effects of antibacterial compounds produced by Saccharomyces cerevisiae in Koumiss on pathogenic Escherichia coli O8 and its cell surface characteristics were *** isolated from Koumiss produced antibacterial compounds which were active against pathogenic *** O8 as determined by the Oxford cup *** aqueous phases from *** at p H=2.0（S2）and pH=8.0（S8）were extracted and tested,*** organic acids of S2 and S8 were determined by high performance liquid chromatography（HPLC）,and the concentrations of killer toxins were determined by enhanced bicinchoninic acid（BCA）Protein Assay *** minimum inhibition concentration（MIC）and the minimum bactericidal concentration（MBC）of S2 and S8 on *** O8 were determined by the broth microdilution *** effects of S2 and S8 on the growth curve of *** O8 were determined by turbidimetry,and the hydrophobicities of *** O8 cell surface were determined using the microbial adhesion to solvents method,the permeation of *** O8 cell membrane were determined by the o-nitrophenyl-β-D-galactoside（ONPG）*** phases at pH 2.0 and 8.0had larger inhibition zones and then S2 and S8 were obtained by *** main component in S2 was citric acid and it was propanoic acid in *** organic acids and killer toxins were also *** the MICs of S2 and S8 on *** O8 were 0.025 g m L^-1,the MBCs were 0.100 and 0.200 g m L^-1,*** normal growth curve of *** O8was S-shaped,however,it changed after addition of S2 and *** O8 was the basic character,and had a relatively hydrophilic *** hydrophobicity of *** O8 cell surface and the permeation of *** O8 cell membrane were increased after adding S2 and *** present study showed that S2 and S8 inhibit the growth of pathogenic *** O8and influence its cell surface characteristics.

关键词： antibacterial compound Saccharomyces cerevisiae Escherichia coil hydrophobicity permeation

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Effects of aggregate size distribution and carbon nanotubes on the mechanical properties of cemented gangue backfill samples under true triaxial compression

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 311-324页

作者： qian Yin Fan wen Zhigang Tao Hai Pu Tianci Deng Yaoyao Meng Qingbin Meng Hongwen Jing Bo Meng Jiangyu Wu State Key Laboratory of Intelligent Construction and Healthy Operation and Maintenance of Deep Underground Engineering China University of Mining and TechnologyXuzhou 221116China State Key Laboratory of Mining Response and Disaster Prevention and Control in Deep Coal Mines Anhui University of Science and TechnologyHuainan 232001China State Key Laboratory for Geomechanics&Deep Underground Engineering China University of Mining and Technology(Beijing)Beijing 100083China Key Laboratory of Xinjiang Coal Resources Green Mining Ministry of EducationXinjiang Institute of EngineeringUrumqi 830023China

The mechanical behavior of cemented gangue backfill materials(CGBMs)is closely related to particle size distribution(PSD)of aggregates and properties of cementitious ***,the true triaxial compression tests,CT scanning,SEM,and EDS tests were conducted on cemented gangue backfill samples(CGBSs)with various carbon nanotube concentrations(P_(CNT))that satisfied fractal theory for the PSD of *** mechanical properties,energy dissipations,and failure mechanisms of the CGBSs under true triaxial compression were systematically *** results indicate that appropriate carbon nanotubes(CNTs)effectively enhance the mechanical properties and energy dissipations of CGBSs through micropore filling and microcrack bridging,and the optimal effect appears at P_(CNT)of 0.08wt%.Taking PSD fractal dimension(D)of 2.500 as an example,compared to that of CGBS without CNT,the peak strength(σ_(p)),axial peak strain(ε_(1,p)),elastic strain energy(Ue),and dissipated energy(U_(d))increased by 12.76%,29.60%,19.05%,and90.39%,***,excessive CNTs can reduce the mechanical properties of CGBSs due to CNT agglomeration,manifesting a decrease inρ_(p),ε_(1,p),and the volumetric strain increment(Δε_(v))when P_(CNT)increases from 0.08wt%to 0.12wt%.Moreover,the addition of CNTs improved the integrity of CGBS after macroscopic failure,and crack extension in CGBSs appeared in two modes:detour and pass through the ***σ_(p)and U_(d)firstly increase and then decrease with increasing D,and porosity shows the opposite ***ε_(1,p)andΔε_(v)are negatively correlated with D,and CGBS with D=2.150 has the maximum deformation parameters(ε_(1,p)=0.05079,Δε_(v)=0.01990)due to the frictional slip effect caused by coarse *** increasing D,the failure modes of CGBSs are sequentially manifested as oblique shear failure,"Y-shaped"shear failure,and conjugate shear failure.

关键词： cemented gangue backfill materials particle size distribution true triaxial compression test carbon nanotubes mechanical properties failure modes

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钠铝氟化物包覆BaF_2材料上转换发光性能

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光谱学与光谱分析 2012年第1期32卷 61-64页

作者：刘恩周樊君胡晓云叶岩溪侯文倩西北大学化工学院陕西西安710069 西北大学物理学系陕西西安710069

采用水热法合成了一种非稀土上转换发光材料,由钠铝氟化物和BaF2组成。采用X射线衍射(XRD)、傅里叶红外光谱(FTIR)和扫描电镜(SEM)对其进行了表征,分析表明,钠铝氟化物主要以NaF和Na3AlF6两种晶态存在于BaF2晶体表面,形成化学复合,呈现... 详细信息

采用水热法合成了一种非稀土上转换发光材料,由钠铝氟化物和BaF2组成。采用X射线衍射(XRD)、傅里叶红外光谱(FTIR)和扫描电镜(SEM)对其进行了表征,分析表明,钠铝氟化物主要以NaF和Na3AlF6两种晶态存在于BaF2晶体表面,形成化学复合,呈现包覆结构。荧光光谱测试表明,样品在583nm可见光和863nm近红外光室温激发下,均能产生304和324nm的宽波段紫外光发射。时间分辨光谱表明,发光均有一个上升和衰减过程,衰减大约自15ms开始,磷光寿命大于18.4ms。借助上转换发光能量转换过程对发光机理进行了研究,分析表明,发光源于量子限制效应的带间光吸收和界面缺陷的光吸收,发光机制属于量子限制效应-界面发光中心复合发光。

关键词：氟化钡包覆结构上转换发光材料磷光寿命发光特性

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双流路顶空热脱附-气相色谱质谱联用测定水中土臭素和二甲基异莰醇

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化学研究与应用 2024年第9期36卷 2203-2209页

作者：王炼侯雯倩陈相何洪志成都市疾病预防控制中心四川成都610041 成都科林分析技术有限公司四川成都610041

建立双流路顶空热脱附-气相色谱质谱联用测定水中土臭素和2-甲基异莰醇的方法。采用双流路顶空提取针将顶空气体进行连续吹扫至冷阱吸附,快速升温解吸,载气反向将待测组分送入气相色谱质谱联用仪分析。对恒温时间、温度等实验条件进行... 详细信息

建立双流路顶空热脱附-气相色谱质谱联用测定水中土臭素和2-甲基异莰醇的方法。采用双流路顶空提取针将顶空气体进行连续吹扫至冷阱吸附,快速升温解吸,载气反向将待测组分送入气相色谱质谱联用仪分析。对恒温时间、温度等实验条件进行了优化,内标法定量,土臭素和2-甲基异莰醇在2.0~100ng·L^(-1)线性回归相关系数≥0.999,方法最低检出浓度为0.74ng·L^(-1)和1.50ng·L^(-1),5ng·L^(-1)、20ng·L^(-1)和100ng·L^(-1)三个浓度加标回收率在90.3~105%,精密度在1.76~6.91%。方法简便、灵敏、重现性好,可用于环境水和生活饮用水中土臭素和2-甲基异莰醇检测。

关键词：顶空热脱附气相色谱质谱联用土臭素 2-甲基异莰醇

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负积温推求冰盖厚度的方法研究

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东北水利水电 2024年第4期42卷 12-13,37,71页

作者：侯倩文吴博松辽水利委员会流域规划与政策研究中心吉林长春130021

本文通过低温模拟渠道结冰试验数据,对两种计算冰盖厚度的斯蒂芬模型和朱波夫模型分别进行了拟合,通过分析发现,拟合出的朱波夫模型计算冰盖厚度与实测冰厚数值更为接近,因此,对于南水北调工程冬季渠道的冰盖厚度,可使用朱波夫模型经验... 详细信息

本文通过低温模拟渠道结冰试验数据,对两种计算冰盖厚度的斯蒂芬模型和朱波夫模型分别进行了拟合,通过分析发现,拟合出的朱波夫模型计算冰盖厚度与实测冰厚数值更为接近,因此,对于南水北调工程冬季渠道的冰盖厚度,可使用朱波夫模型经验公式进行预测,以进一步计算冰盖膨胀力,防止膨胀力过大对渠道边坡稳定性造成威胁。

关键词：负积温冰盖厚度模型

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松花江流域中小河流治理现状及存在问题探讨

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东北水利水电 2024年第7期42卷 30-31页

作者：侯倩文陈娟松辽水利委员会流域规划与政策研究中心吉林长春130021

松花江流域中小河流量多面广、防洪任务重。本文通过相关数据分析了流域内200~3 000 km^(2)中小河流治理现状,探讨目前中小河流治理存在的问题,为下阶段中小河流治理提供参考。

关键词：中小河流治理现状松辽流域

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Identification of a dual functional betulinic acid analog for the treatment of osteoarthritis by phenotypic screening

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Science China Life Sciences 2025年第1期68卷 300-302页

作者： Ze-Min Lin Mai Xiang wen-Hui Wei Shu-Hui Fan Li Chen Jie Wang Xiao-qian Yang Chun-Hao Yang Shi-Jun He Jian-Ping Zuo Laboratory of Immunopharmacology State Key Laboratory of Drug ResearchShanghai Institute of Materia MedicaChinese Academy of Sciences School of Pharmacy University of Chinese Academy of Sciences School of Pharmacy Nanjing University of Chinese Medicine State Key Laboratory of Drug Research Department of Medicinal ChemistryShanghai Institute of Materia MedicaChinese Academy of Sciences Innovation Research Institute of Traditional Chinese Medicine Shanghai University of Traditional Chinese Medicine Experiment Center for Science and Technology Shanghai University of Traditional Chinese Medicine

Dear Editor, Phenotypic drug discovery, which observes compound effects on physiology,has given way to targeting specific molecules, but has revived by using modern methods to find drugs based on therapeutic outcomes in disease models(Vincent et al., 2022). Osteoarthritis(OA) is a prevalent chronic joint disorder, primarily affecting knee joints, with osteoclast activation due to low-grade inflammation resulting in increased bone resorption and structural changes in the joint(Katz et al.,2021).

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不同贮藏条件下小麦和玉米种子的水分变化规律及其建模验证

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作物学报 2011年第1期37卷 158-164页

作者：王婧李晓丽姜朋田凤龙侯文倩孙庆泉山东农业大学农学院作物生物学国家重点实验室/山东省作物生物学重点实验室山东泰安271018

种子平衡水分(EMC)是评价种子吸湿或解吸动态变化的重要指标。本研究以不同初始水分(IMC)小麦和玉米种子为试材,研究了不同贮藏条件下的吸湿解吸规律,并建模验证。结果表明,玉米种子在8.0%IMC、15℃、相对湿度(RH)50.0%、25℃和RH>50.0%... 详细信息

种子平衡水分(EMC)是评价种子吸湿或解吸动态变化的重要指标。本研究以不同初始水分(IMC)小麦和玉米种子为试材,研究了不同贮藏条件下的吸湿解吸规律,并建模验证。结果表明,玉米种子在8.0%IMC、15℃、相对湿度(RH)50.0%、25℃和RH>50.0%、40℃和RH>48.0%时超过其对应条件下的SWC;玉米种子EMC在15℃和RH>50.0%、25℃和RH>47.0%以及40℃和RH>47.0%时超过其对应条件下的SWC。玉米郑单958的平衡时间(d)与IMC(x)、RH(y)和温度(z)的预测模型为d=35.34+4.32x-0.28y-0.35z-0.031xy-0.012xz-0.0011yz+0.003y2,农大108为d=39.76+2.35x-0.39y-0.27z-0.022xy-0.014xz-0.0057yz+0.008y2,小麦山农15为d=25.69+7.65x-0.27y-0.89z-0.12xy-0.07xz-0.007yz+0.006y2+0.04z2,泰农18为d=46.67+0.39x-0.28y-1.72z-0.006yz+0.006y2+0.017z2。模型经验证,预测性良好。

关键词：小麦种子玉米种子吸湿解吸建模模型验证

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