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应用人工合成的hIV nef蛋白肽制备抗nef单抗

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免疫学杂志 1990年第3期6卷 153-157页

作者：史良如 J.Enzman M.Schneider h.Brüster *** 卫生部武汉生物制品研究所免疫学研究室武汉430031 Institute for transfusion Medicine Uni ClinicDüsseldorfFRG

本文用人工合成的hIV nef蛋白肽免疫Belb/c小鼠,应用活体融合法制备了11个抗nef单克隆抗体。ELISA测定表明,11个单抗均能与nef肽和nef蛋白反应,其中3、7、9、10和11号抗体与nef肽和nef蛋白的反应性相近似;4、5、6和7号抗体与nef蛋白的... 详细信息

本文用人工合成的hIV nef蛋白肽免疫Belb/c小鼠,应用活体融合法制备了11个抗nef单克隆抗体。ELISA测定表明,11个单抗均能与nef肽和nef蛋白反应,其中3、7、9、10和11号抗体与nef肽和nef蛋白的反应性相近似;4、5、6和7号抗体与nef蛋白的反应性明显比肽强。所有单抗均不与hIV的其它蛋白反应。应用免疫荧光检测抗nef单抗与hIV感染细胞的反应性表明,大部分抗体均与细胞浆着色,而无一般膜荧光的典型征象。

关键词： hIV AIDS nef肽单克隆抗体

中层顶波非线性相互作用的甚高频雷达观测研究

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电波科学学报 2000年第1期15卷 84-89页

作者：熊建刚易帆 J.Klostermeyer R.Rüster 中国科学院武汉物理与数学研究所武汉大学无线电科学技术系武汉430072 Max-Planck- Institutefür Aeronomic Max-Planck-Institutefür Aeronomic

利用 SOUSY VhF雷达 1 987年 6月 2 2日至 6月 2 9日在挪威 Andoya的观测数据研究了中层顶附近大气风场中行星波、潮汐和惯性重力波的非线性相互作用。首先利用 Lomb- Scargle周期图方法计算了大气纬向风场的功率谱 ,除了行星波和潮汐... 详细信息

利用 SOUSY VhF雷达 1 987年 6月 2 2日至 6月 2 9日在挪威 Andoya的观测数据研究了中层顶附近大气风场中行星波、潮汐和惯性重力波的非线性相互作用。首先利用 Lomb- Scargle周期图方法计算了大气纬向风场的功率谱 ,除了行星波和潮汐外 ,在行星波、潮汐的频率和与频率差处有明显的谱峰 ,双谱分析表明这些谱峰是行星波和潮汐相互作用形成的。在 86.4km处 35h行星波、半日潮与 8.9h惯性重力波有较强的非线性耦合 ,半日潮与行星波间有明显的能量传输。半日潮振幅变化的周期为 78h,远大于行星波的周期。 85.2 km处经向风场半日潮振幅的变化周期同样大于与之相互作用的 33h行星波的周期。

关键词：行星波潮汐中层顶波甚高频雷达惯性重力波

基于人群筛查的数字钼靶摄影与乳腺体层摄影中乳腺密度对诊断性能的影响

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国际医学放射学杂志 2019年第6期 734-735页

作者： B.h.?ster?s A.C.T.Martinsen R.Gullien P.Skaane 徐文达 Department of Diagnostic Physics and Division of Radiology and Nuclear Medicine Oslo University Hospital

摘要目的根据容积密度、年龄和乳腺摄影的结果,比较数字化乳腺摄影(DM)与乳腺体层摄影(DBT)的真阳性和假阳性结果。材料与方法从2010年11月—2012年12月

关键词：乳腺密度年龄组钼靶摄影体层摄影人群筛查

慢性关节炎患者的心理社会适应能力:患有幼年型特发性关节炎和反应性关节炎的儿童和青少年的行为特征

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世界核心医学期刊文摘（儿科学分册） 2006年第11期2卷 61-62页

作者： Müller-Godeffroy E. Lehmann h. Küster R.M. ThyenU 郭战宏 Klinik für Kinder-und Jugendmedizin Universittsklinikum Schleswig-Holstein Campus LübeckRatzeburger Allee 160 23538 Lübeck Germany

We sought to measure psychosocial adaptation in children and adolescents with different forms of chronic arthritis and to determine associated factors. Mothers of 68 children aged 8-17 years with juvenile idiopathic arthritis (JIA) or reactive arthritis reported behaviour problems (CBCL), children reported their actual stress level (SSK), functional disability in conducting activities of daily living (ChAQ) and health-related quality of life (hRQOL) including self-esteem (KINDL-R). Children and adolescents with JIA or reactive arthritis reported lower self-esteem compared to normative data. Almost 20%of the samples were reported to have serious behaviour problems,mostly social isolation and depression/anxiety. Children with lower hRQOL, more functional limitations and a higher stress level showed more behaviour problems. Self-esteem and behaviour problems may be relevant outcome measures in children and adolescents with chronic arthritis and useful to monitor psychosocial support in this population.

关键词：特发性关节炎慢性关节炎反应性关节炎社会适应能力幼年型 CBCL 自我评价生活质量功能障碍

Validation of CFD-DEM simulation of a liquid-solid fluidized bed by dynamic analysis of time series

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Particuology 2022年第9期20卷 75-87页

作者： Julia Picabea Mauricio Maestri Miryan Cassanello Gabriel Salierno Cataldo De Blasio María Angélica Cardona Daniel hojman héctor Somacal Departamento de Industrias Facultad de Ciencias Exactas y NaturalesUniversidad de Buenos AiresCiudad UniversitariaIntendente Güiraldes 2160C1428EGABuenos AiresArgentina CONICET-Universidad de Buenos Aires Instituto de Tecnología de Alimentos y Procesos Químicos-ITAPROQCiudad UniversitariaIntendente Güiraldes 2160 C1428EGABuenos AiresArgentina Faculty of Science and Engineering Abo Akademi UniversityRantakatu 265100 VaasaFinland Laboratorio de Diagnósticos por Radiaciones Dep.Física ExperimentalComisión Nacional de Energía AtómicaBKNA1650 San MartínBuenos AiresArgentina CONICET C1033AAJ Buenos AiresArgentina Escuela de Ciencia y Tecnología Universidad de San MartínBKNA 1650 San MartínBuenos AiresArgentina

Liquid-solid fluidized beds(LSFB)modeling validation is crucial for establishing design rules and monitor-ing ***,it generally relies on comparing global variables,which overlook dynamic features that influence reaction *** work aims to implement time series analysis tools to compare Radioactive Particle Tracking data with a simulation consisting of Computational Fluid Dynamics cou-pled with Discrete-Element *** have been performed in a pilot-scale LSFB of calcium alginate spheres fluidized with a calcium chloride *** Diks'test indicates that the simulation can capture the LSFB *** also allows diagnosing flow regime transitions from the *** of solid dispersion coefficients and mixing times predicted by the simulation are in good agreement with the experiments.

关键词： Liquid-solid fluidization CFD-DEM Dynamic comparison Radioactive particle tracking

比较传统钼靶与钼靶+断层融合技术在乳腺癌筛查中的应用:Oslo断层成像试验

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国际医学放射学杂志 2019年第3期 360-360页

作者：景瑞 P.Skaane A.I.BANDos L.T.Niklason S.SebuΦdeg?rd B.h.Φster?s R.Gullien Division of Radiology and Nuclear Medicine Oslo University HospitalUniversity of OsloBreast Imaging Center

摘要在临床工作中数字乳腺断层摄影(DBT)正逐渐取代数字乳腺钼靶(DM)检查。针对上述2项检查的准确性以及合成融合钼靶摄影(SM)和计算机辅助检测(CAD)的作用,

关键词：乳腺癌筛查特异度敏感度 DBT DM

电子血糖分析仪用于新生儿低血糖床旁测定可能不十分可靠

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世界核心医学期刊文摘（儿科学分册） 2006年第6期2卷 19-19页

作者： Rosenthal M. Ugele B. Lipowsky G. Küster h. 刘莉 Department for Neonatology and Pediatric Intensive Care University Children's Hospital Soldtmannstr. 15 17487 Greifswald Germany

The aim of this prospective observational study was to compare a bedside test with the reference laboratory method in routine postnatal glucose monitoring. Term newborns with increased risk or clinical signs of hypoglycemia were screened with a bedside test. In case of a glucose value below 2.25 mmolL, a second blood sample was taken and a duplicate glucose measurement done in the laboratory using a bedside test (Accutrend sensor) and the reference laboratorymethod (hexokinase method) at the same time and from the same sample. From 110 term newborns, 122 blood samples were obtained for duplicate measurements (median 1.69 mmolL, SD 0.45 mmolL). Of these 122, Accutrend correctly identified 97%as being he laboratory method. A Bland-Altman plot revealed a mean underestimation of the Accutrend of only-0.09 mmolL. however, due to high scattering, the maximal over-and underestimation was 0.89 and 1.39 mmolL, respectively. Only 75%of the results from the Accutrend were within ±20%of the result of the laboratory method. If the cutoff for low glucose concentrations was set 0.6 mmolL higher for the bedside test as compared to the laboratory method, all patients except one would have been correctly identified as hypoglycemic. Conclusion: When using the Accutrend sensor, single infants with even marked hypoglycemia might be missed. Some delay in receiving accurate measurements might be more helpful for clinical decisions and long-term outcome than immediate but potentially misleading results.

关键词：新生儿低血糖血糖测定值床旁检测分析仪 0.6mmol/L 电子实验室方法实验室检查方法足月新生儿前瞻性观察

Testing of the Adhesion of herpes Simplex Virus on Textile Substrates and Its Inactivation by household Laundry Processes

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Journal of Biosciences and Medicines 2016年第12期4卷 111-125页

作者： Anja Gerhardts Dirk Bockmühl Andrea Kyas Anja hofmann Mirko Weide Ingrid Rapp Dirk höfer William-Kü ster-Institut for hygiene Environment & Medicine Hohenstein Institute for Textilinnovation gGmbH Boen-nigheim Germany Rhine-Waal University of Applied Sciences Hygiene and Microbiology Kleve Germany henkel AG & Co. KGaA Corporate Scientific Services Duesseldorf Germany Labor Dr. Merk & Kollegen Gmbh Ochsenhausen Germany henkel AG & Co. KGaA International R&D Laundry & Home Care Duesseldorf Germany

Viral infections like herpes simplex increasingly pose a serious threat to European health care systems and welfare of the population. Indirect transmission routes of infections via inanimate surfaces are often underestimated. In this study, we investigated the adhesion and persistence of herpes simplex virus on cotton fabrics as well as its inactivation by domestic laundry. Virus adhesion to textile fibers was distinct, because viral DNA was detectable on fabrics for at least 48 hours after contamination as well as after home laundry. Particles remained infectious for several hours at room temperature and partially for 48 hours at 2℃ - 8℃. Nevertheless, domestic laundry was able to inactivate virus particles given that detergents were adequately used. This confirmed that standard household laundry processes, as established in Europe, are a suitable tool to reduce infectious herpes virus particles from textiles, thereby supporting the prevention of infections circulating in the household and community.

关键词： home Laundry herpes Simplex Disinfectant Laundry Processes Inactivation of Viruses

电碳酸氢钠协同转运蛋白1(NBCe1)在小鼠皮质星形胶质细胞中的功能性表达依赖于S255-257并受mTOR调节

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神经损伤与功能重建 2019年第7期14卷 F0003-F0003页

作者： Khakipoor S Giannaki M Theparambil SM Zecha J Küster B heermann S Deitmer JW Roussa E 杜一星(编译) Department of Molecular Embryology Institute for Anatomy and Cell Biology Faculty of Medicine University of Freiburg Albertstrasse 17 D-79104 Freiburg Germany Department of Neuroanatomy Institute for Anatomy and Cell Biology Faculty of Medicine University of Freiburg Albertstrasse 17 D-79104 Freiburg Germany Department of General Zoology FB Biology University of Kaiserslautern P.B. 3049 D-67653 Kaiserslautern Germany 不详

电碳酸氢钠协同转运蛋白1,NBCe1(SLC4A4)是星形胶质细胞中表达的主要碳酸氢根转运蛋白。它对碳酸氢根高度敏感,并且是细胞内、细胞外和突触ph值的主要调节剂,从而调节神经元兴奋性。尽管已知NBCe1的这些基本功能,然而NBCe1介导的星形胶... 详细信息

电碳酸氢钠协同转运蛋白1,NBCe1(SLC4A4)是星形胶质细胞中表达的主要碳酸氢根转运蛋白。它对碳酸氢根高度敏感,并且是细胞内、细胞外和突触ph值的主要调节剂,从而调节神经元兴奋性。尽管已知NBCe1的这些基本功能,然而NBCe1介导的星形胶质细胞对细胞外ph值变化反应的分子机制尚不明确。使用原代小鼠皮质星形胶质细胞培养,本研究探索长期细胞外代谢碱中毒对NBCe1调节的影响;并通过免疫印迹,表面蛋白的生物素化,使用h+敏感染料2,7-双(羧乙基)-5-(6)-羧基荧光素记录细胞内h+,和磷酸化蛋白质组学分析阐明该调节作用潜在的分子机制。结果显示代谢性碱中毒后NBCe1活性显著下调,而NBCe1的蛋白质丰度或表面表达并不受影响。在碱中毒期间,NBCe1活性下调时细胞内h+变化的速率在野生型星形胶质细胞中降低,但在NBCe1缺陷小鼠的皮质星形胶质细胞中不变。激活mTOR信号传导能够拯救碱中毒诱导的NBCe1活性降低。此外,质谱分析揭示S255-257的组成型磷酸化,而突变分析揭示这些残基对NBCe1转运活性至关重要。本研究证明了一种通过调节NBCe1的功能表达而实现的新的mTOR调节机制。该种机制不仅适用于星形胶质细胞,也适用于上皮细胞中的NBCe1。

关键词：星形胶质细胞癫痫生长因子 ph值