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Phylogenetic diversity of planktonic bacteria in the Chukchi Borderland region in summer

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Acta Oceanologica Sinica 2013年第6期32卷 66-74页

作者： ZENG Yinxin YU Yong LI huirong hE Jianfeng LEE Sang h SUN Kun Key Laboratory for Polar Science of State Oceanic Administration Polar Research Institute of China College of Biological Engineering Jimei University Department of Oceanography Pusan National University

Planktonic bacteria are abundant in the Chukchi Borderland region. however, little is known about their di- versity and the roles of various bacteria in the ocean. Seawater samples were collected from two stations K2S and K4S where sea ice was melting obviously. The analysis of water samples with fluorescence in situ hybridization （FIsh） showed that DMSP-degrading bacteria accounted for 13% of the total bacteria at the station K2S. No aerobic anoxygenic phototrophic （AAP） bacteria were detected in both samples. The bacterial communities were characterized by two 16S rRNA gene clone libraries. Sequences fell into four major lineages of the domain Bacteria, including Proteobacteria （Alpha, Beta and Gamma subclasses）, Bac- teroidetes, Actinobacteria and Firmicutes. No significant difference was found between the two clone li- braries. SAR11 and Rhodobacteraceae clades of Alphaproteobacteria and Pseudoalteromonas of Gammapro- teobacteria constituted three dominant fractions in the clone libraries. A total of 191 heterotrophic bacterial strains were isolated and 76% showed extracellular proteolytic activity. Phylogenetic analysis reveals that the isolates fell into Gammaproteobacteria, Bacteroidetes, Actinobacteria and Firmicutes. The most common genus in both the bacterial isolates and protease-producing bacteria was Pseudoalteromonas. UniFrac data showed suggestive differences in bacterial communities between the Chukchi Borderland and the northern Bering Sea.

关键词： diversity planktonic bacteria 16S rRNA gene clone library Chukchi Borderland

Epitope DNA vaccines against tuberculosis： spacers and ubiquitin modulates cellular immune responses elicited by epitope DNA vaccine

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第二军医大学学报 2005年第2期26卷 191-191页

作者： Wang QM Sun sh hu ZL Zhou FJ Yin M Xiao CJ Zhang JC Department of Medical Genetics Second Military Medical University Shanghai 200433 China

Cell-mediated immune responses are crucial in the protection against tuberculosis. In this study, we constructed epitope DNA vaccines (p3-M-38) encoding cytotoxic T lymphocyte (CTL) epitopes of MPT64 and 38 kDa proteins of Mycobacterium tuberculosis. In order to observe the influence of spacer sequence (Ala-Ala-Tyr) or ubiquitin (UbGR) on the efficacy of the two CTL epitopes, we also constructed DNA vaccines, p3-M-S(spacer)-38, p3-Ub (UbGR)-M-S-38 and p3-Ub-M-38. The immune responses elicited by the four DNA vaccines were tested in C57BL/6 (h-2b) mice. The cytotoxicity of T cells was detected by LDh-release method and by enzyme-linked immunospot assay for epitope-specific cells secreting interferon-gamma. The results showed that DNA immunization with p3-M-38 vaccine could induce epitope-specific CD8+ CTL response and that the spacer sequence (AAY) only enhanced M epitope presentation. The protein-targeting sequence (UbGR) enhanced the immunogenicity of the two epitopes. The finding that defined spacer sequences at C-terminus and protein-targeting degradation modulated the immune response of epitope string DNA vaccines will be of importance for the further development of multi-epitope DNA vaccines against tuberculosis.

关键词： DNA vaccines immune responses Mycobacterium tuberculosis cytotoxic T lymphocyte protection against DNA immunization T cells

Reply to comment by S.h.Büttner on “Evidence for Mesoproterozoic collision,deep burial and rapid exhumation of garbenschiefer in the Namaqua Front,South Africa”

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Geoscience Frontiers 2020年第5期11卷 1895-1899页

作者： Valby van Schijndel David h.Cornell Robert Anczkiewicz Anders Schersten Institute of Geosciences University of Potsdam14476PotsdamGermany Department of Earth Sciences University of GothenburgSE-40530GothenburgSweden Department of Earth Sciences University of StellenboschMatieland7602South Africa Institute of Geological Sciences Polish Academy of SciencesKrakow Research Centre31-002KrakowPoland Lund University Department of GeologySE-22362LundSweden

*** We thank *** for his comments on our paper(van Schijndel et al.,2020),which allow us to clarify our work and the tectonic model we proposed for the eastern margin of the Namaqua Sector of the Namaqua Natal Metamorphic *** Kaaien Terrane forms a tectonic transition between the Kaapvaal Craton,Rehoboth Province,Kheis belt and the Archeachap Terrane of the Namaqua Sector and forms part of a NW-vergent foreland thrust sequence in the Namaqua Front(*** et al.,2006).The exact tectonic extent of the Kaaien Terrane is still unclear and it has recently been proposed to be part of the newly defined Kheis Terrane(Van Niekerk and Beukes,2019).

关键词： Province. collision comment

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三氧化二砷治疗肝癌的分子机制

世界华人消化杂志 2005年第14期13卷 1737-1743页

作者：吴顺华成军郑玉建北京地坛医院传染病研究所北京市100011 新疆医科大学公共卫生学院劳动卫生与环境卫生学教研室

三氧化二砷(As2O3)在白血病和各类实体瘤的治疗中广泛应用.肝细胞肝癌(hepatocellular carcinoma,hCC)是消化系统常见的肿瘤.其恶性程度高、预后差.As2O3在与肝癌细胞的研究表明,他可以发挥细胞凋亡的正向调控作用,抑制肿瘤细胞增殖,是... 详细信息

三氧化二砷(As2O3)在白血病和各类实体瘤的治疗中广泛应用.肝细胞肝癌(hepatocellular carcinoma,hCC)是消化系统常见的肿瘤.其恶性程度高、预后差.As2O3在与肝癌细胞的研究表明,他可以发挥细胞凋亡的正向调控作用,抑制肿瘤细胞增殖,是较好的抗肿瘤药物之一.但As2O3同时作为一种致癌物,与含巯基的大分子蛋白结合,影响细胞的正常代谢,导致细胞内信号转导通路异常调节,抑癌基因失活等,从而引起皮肤异常改变和其他内脏肿瘤如肝癌、肺癌、膀胱癌的发生.因此研究As2O3对肝细胞作用的机制对有效防治hCC有很重要的意义.本文从As2O3的生物学抗癌效应入手综述其治疗肝癌的分子机制.

关键词：三氧化二砷治疗肝癌分子机制

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碘缺乏地区甲低病人的抑制型免疫球蛋白

天津医药 1990年第11期18卷 656-658页

作者：汤特王荫谷马泰 Ingbar sh 天津医学院病理生理学教研室新疆阿克苏地方病办公室美国哈佛大学医学院附属医院内分泌研究室

我们对地方性克汀病(地克病)病人血清中 IgG 对甲低和甲状腺萎缩的病因发病学进行了研究。在新疆阿克苏地区检测了20例无甲状腺肿大的地克病病人,粘肿型8例,神经型5例,混合型7例,其中7例粘肿型,1例混合型病人血清中 Tsh 水平增高,T_4降... 详细信息

我们对地方性克汀病(地克病)病人血清中 IgG 对甲低和甲状腺萎缩的病因发病学进行了研究。在新疆阿克苏地区检测了20例无甲状腺肿大的地克病病人,粘肿型8例,神经型5例,混合型7例,其中7例粘肿型,1例混合型病人血清中 Tsh 水平增高,T_4降低(?)1例混合型病人高 Tsh,而 T_4值在正常范围(亚临床甲低),其余病人为甲状腺功能正常或怀疑甲亢者。通过对病人血清 IgG 纯化后,按4mg/ml 在大鼠甲状腺细胞(FRTL-5)中抑制~3h-胸腺嘧啶核苷掺入DNA 的作用,测定甲状腺生长抑制免疫球蛋白(TGII),结果在上述9例病人血清中有6例(66.7%)TGII 阳性,其它各种类型的病人均为阴性。此外也测定了其它抑制生长的 IgG,如对IGF-I 抑制~3h-胸腺嘧啶核苷掺入 DNA 的作用。结果在上述9例病人中有5例(55.6%)有抑制胰岛素样生长因子 I 的(IGF-I blocking)IgG。上述结果指出,尽管血清中 Tsh 水平升高,TGII 确是引起甲状腺萎缩的原因之一,同时抑制 IGF-I 诱发甲状腺生长的 IgG。

关键词：克汀病甲低免疫球蛋白

Unraveling the morphological patterns of a subantarctic eelpout:a geometric morphometric approach

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Integrative Zoology 2023年第2期18卷 372-384页

作者： Fernanda S.ORREGO Mathias hÜNE hugo A.BENÍTEZ Mauricio F.LANDAETA Laboratorio de Ictiología e Interacciones Biofísicas(LABITI) Instituto de BiologíaFacultad de CienciasUniversidad de ValparaísoValparaísoChile Centro de Investigación para la Conservación de los Ecosistemas Australes(ICEA) Punta ArenasChile Laboratorio de Ecología y Morfometría Evolutiva Centro de Investigación de Estudios Avanzados del MauleUniversidad Católica del MauleTalcaChile Centro de Observación Marino para Estudios de Riesgos del Ambiente Costero(COSTA-R) Universidad de ValparaísoViña del MarChile Millennium Nucleus for Ecology and Conservation of Temperate Mesophotic Reef Ecosystems(NUTME) Millennium Institute Biodiversity of Antarctic and Subantarctic Ecosystems(BASE) SantiagoChile

Phenotypic variation in organisms depends on the genotype and the environmental constraints of the habitat that they ***,for marine species inhabiting contrasting aquatic conditions,it is expected toﬁnd covari-ation between the shape and its spatial *** studied the morphology of the head and cephalic sensory canals of the eelpout Austrolycus depressiceps(4.5–22.5 cm TL)across its latitudinal distribution in South Paciﬁc(45°S–55°S).Geometric morphometric analyses show that the shape varied from individuals with larger snout and an extended suborbital canal to individuals with shorter snouts and frontally compressed suborbital *** was size variation across the sampled populations,but that size does not have a clear latitudinal *** 8%of the shape variation relates to this size variation(allometry),represented by a decrease in the relative size of the eye,and a depression of the posterior margin of the *** were spatial differences in the shape of the head,but these differences were probably caused by allometric ***,2 of the canals of the cephalic lateralis pores and the head shape showed modularity in its *** study shows that the morphology of marineﬁsh with a shallow distribution varies across distances of hundreds of kilometers(i.e.,phenotypic modulation).

关键词： Austrolycus depressiceps geometric morphometrics morphology phenotype spatial distribution

使用OprF作为锚定蛋白在大肠杆菌表面展示脂肪酶及在有机溶剂中筛选对映异构体的应用

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中国医药工业杂志 2005年第11期36卷 659-659页

作者： Lee sh 陈丹（译）胡又佳（校）

开发了一种新的细胞表面展示系统，使用铜绿假单孢菌的一种主要外膜蛋白OprF作为锚定位点。荧光假单孢菌SIK W1脂肪酶基因通过C端删除融合技术与截短的oprF基因融合。在大肠杆菌表面成功展示了截短的OprF-脂肪酶融合蛋白。通过蛋白杂交... 详细信息

开发了一种新的细胞表面展示系统，使用铜绿假单孢菌的一种主要外膜蛋白OprF作为锚定位点。荧光假单孢菌SIK W1脂肪酶基因通过C端删除融合技术与截短的oprF基因融合。在大肠杆菌表面成功展示了截短的OprF-脂肪酶融合蛋白。通过蛋白杂交分析、免疫荧光显微术和整个细胞的脂肪酶活性确定了截短的OprF-脂肪酶融合蛋白在膜上的定位。为了检测细胞表面展示脂肪酶的酶学特征，在各种条件下考查了细胞整体酶活力和稳定性。细胞表面展示脂肪酶在37℃、ph 8．0时酶活力最高。在水溶液和有机溶液中温育一周后，酶活力还可保留80％以上。将展示脂肪酶的大肠杆菌用于外消旋1-苯基乙醇在正己烷中的对映异构体选择，经36h反应成功获得了对映体超过96％的（R）-苯乙酸乙酯。结果表明OprF作锚定蛋白展示脂肪酶的大肠杆菌可用于各种水相、非水相的生物技术应用。

关键词：表面展示系统脂肪酶活性生物技术应用对映异构体大肠杆菌锚定蛋白有机溶剂筛选铜绿假单孢菌

钯催化芳香重氮盐和芳香亚磺酸盐生成不对称联芳烃的偶联反应

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中国医药工业杂志 2017年第11期48卷 1596-1596页

作者： gund sh 郑楠

重氮苯四氟硼酸盐在Pd（dppf）Cl2（2．5％mol）催化下，以P（OPh），（5％mol）为配体、碳酸钾（1．25倍摩尔量）为碱，四丁基碘化铵（1倍摩尔量）为碘源，与苯亚磺酸钠在DMF中于氮气保护下150℃反应24h，生成相应的不对称联芳烃。14... 详细信息

重氮苯四氟硼酸盐在Pd（dppf）Cl2（2．5％mol）催化下，以P（OPh），（5％mol）为配体、碳酸钾（1．25倍摩尔量）为碱，四丁基碘化铵（1倍摩尔量）为碘源，与苯亚磺酸钠在DMF中于氮气保护下150℃反应24h，生成相应的不对称联芳烃。14例收率73％～84％。重氮盐芳环上连有给电子基团的比吸电子基团收率高，且对位取代比邻位或间位取代收率高。

关键词：偶联反应重氮盐不对称钯催化芳烃磺酸盐芳香四丁基碘化铵

Systematic review of anorectal leiomyosarcoma: Current challenges and recent advances

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World Journal of Gastrointestinal Surgery 2019年第8期11卷 334-341页

作者： Mohammed O Nassif Razan A habib Luai Z Almarzouki Nora h Trabulsi Department of Surgery King Abdulaziz University

BACKGROUND The anorectal leiomyosarcoma (LMS) is an aggressive malignant neoplasm. Owing to the rarity of LMSs, an optimal treatment modality has yet to be determined. AIM To collect all published data on anorectal LMS characteristics, explore current treatment options, and review recent cases of postradiation LMS. METhODS A literature search of the PubMed electronic database was conducted using the Mesh terms "rectal neoplasms","anus neoplasms" and "gastrointestinal neoplasms" combined with "leiomyosarcoma". The search was limited to English language and human studies. All available case reports and case series of anal or rectal LMSs that were published from the beginning of January 1996 to May 2017 were included if the diagnosis of LMS had been confirmed by histopathologic examination. Data were analyzed using simple statistics (mean, median, and standard deviation). Independent sample t-test was used to compare means for continuous variables. RESULTS A total of 27 articles reporting on 51 cases of anorectal LMS were identified. Among these cases, 11.7% had undergone previous pelvic radiotherapy (developing LMS at 13-35 years afterwards). Anorectal LMS affected the rectum in 92.2% of the cases, and no sex-based predominance was observed. Surgical resection with negative margins remains the mainstay of treatment, which can be accomplished with wide local excision or radical resection. The local recurrence rate was higher among cases who received wide local excision (30%), as compared to radical resection (20%);however, the overall rate of metastasis was 51.61% regardless of the treatment approach. The use of neoadjuvant radiation lowers the risk of local recurrence compared to adjuvant radiotherapy, and facilitates R0 resection of the tumor. Cases treated with adjuvant chemotherapy showed better rates of distant recurrence and overall survival. Nonetheless, multidisciplinary team discussion is necessary to determine the optimal management plan whilst considering pa

关键词： Leiomyosarcoma Rectal neoplasms Anal neoplasms Gastrointestinal neoplasms Soft tissue neoplasms