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水稻矮缩病毒最外层外壳蛋白基因(S_2)cDNA克隆、序列分析及其在大肠杆菌中的表达

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微生物学报 1999年第4期39卷 305-314页

作者：鲁瑞芳李毅杨崇林颜华陈章良北京大学生命科学院蛋白质工程及植物基因工程国家重点实验室

从水稻矮缩病毒（Ｒｉｃｅｄｗａｒｆｖｉｒｕｓ，ＲＤＶ）中国福建分离物中克隆分离了最外层外壳蛋白基因（Ｓ２）全长ｃＤＮＡ，并对其进行了序列分析，结果表明ＲＤＶＳ２ｃＤＮＡ全长３５１２ｂｐ，仅含一个３３４８ｂｐ的阅读框架... 详细信息

从水稻矮缩病毒（Ｒｉｃｅｄｗａｒｆｖｉｒｕｓ，ＲＤＶ）中国福建分离物中克隆分离了最外层外壳蛋白基因（Ｓ２）全长ｃＤＮＡ，并对其进行了序列分析，结果表明ＲＤＶＳ２ｃＤＮＡ全长３５１２ｂｐ，仅含一个３３４８ｂｐ的阅读框架，编码一个含有１１１６个氨基酸的蛋白（Ｐ２）。与基因库中已知基因序列比较，发现它与日本ＲＤＶＨ株系相应片段的核苷酸和氨基酸同源率分别为９４６％和９５４％，与轮状病毒ＶＰ２氨基酸序列有一定的同源性。Ｓ２核苷酸序列二级结构预测结果表明，５’端５０个核苷酸的二级结构为一个发夹结构和一个茎环结构。Ｐ２有４个富含亮氨酸的区域，位于Ｎ端亲水区域的１０个氨基酸（ＡＡ６９～７８）残基形成一个α螺旋，这些特点均与轮状病毒ＶＰ２的结构特征相似。ＳＤＳＰＡＧＥ和Ｗｅｓｔｅｒｎ印迹分析表明在大肠杆菌中分段高效表达了Ｓ２编码蛋白的Ｎ端和Ｃ端。

关键词：水稻矮缩病毒外壳蛋白基因序列分析基因表达

Testosterone and Its Bioactive Components Are Associated with Libido and the Metabolic Syndrome in Men

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Advances in Sexual Medicine 2017年第2期7卷 105-119页

作者： h. Alidu N. Amidu W. K. B. A. Owiredu C. K. Gyasi-Sarpong A. T. Bawah P. P. M. Dapare E. B. Agyemang Prempeh Department of Medical Laboratory Science University of Health and Allied Sciences Ho Ghana Department of Biomedical Laboratory Science University for Development Studies Tamale Ghana Department of Molecular Medicine Kwame Nkrumah University of Science & Technology Kumasi Ghana Department of Surgery (Urology) School of Medical Sciences/Komfo Anokye Teaching Hospital Kumasi Ghana Department of Academic Affairs University of Health and Allied Sciences Ho Ghana

Background: Some evidence has shown that the prevalence of hypoactive sexual desire and erectile dysfunction (ED) is associated with testosterone levels whilst higher levels of testosterone have been reported to increase the frequency of intercourse. Available evidence points towards an etiologic role for hypogonadism in the causation of diabetes, metabolic syndrome (Mets) and sexual dysfunction (SD) even though the exact pathophysiological linkage is yet to be fully elucidated. This study therefore sought to evaluate the impact of testosterone as well as its bioactive components on both the MetS and SD. Method: Diabetic men engaged in a stable heterosexual relationship for at least 2 years were recruited for this study. Participants were at least 18 years and provided an informed and signed consent to partake in this study. Fasting blood samples were taken from the participants for biochemical and hormonal assay. The participants were then evaluated using the Golombok Rust Inventory of Sexual Satisfaction for males (GRISS-M). Metabolic syndrome was assessed using the NCEP-ATP III, IDF and WhO criteria. All data analyses were performed using the SPSS software, version 11.0 systat, Inc. Germany and GraphPad Prism, version 5.0, San Diego California, USA. Results: The mean total, free and bioavailable testosterone as well as ShBG recorded among the participants was 7.10 ± 1.23 ng/ml, 0.10 ± 0.01 ng/ml, 7.01 ± 1.41 ng/ml and 4.33 ± 1.12 nmol/l respectively. Subjects with the MetS showed significantly lower ShBG levels in comparison with subjects without the MetS. Participants with raised triglyceride levels showed significantly lower levels of total, free and bioavailable testosterone when compared to participant without raised triglycerides. Participants with problems of avoidance and infrequency of sexual activity showed significantly lower levels of total, free and bioavailable testosterone when compared to participants without avoidance or infrequency problems. The

关键词： hypogonadism Sexual Dysfunction Metabolic Syndrome Type II Diabetes Testosterone

EMP control and characterization on high-power laser systems

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维普期刊数据库

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high Power Laser Science and Engineering 2018年第2期6卷 55-62页

作者： P.Bradford N.C.Woolsey G.G.Scott G.Liao h.Liu Y.zh.ng B.zhu C.Armstrong S.Astbury C.Brenner P.Brummitt F.Consoli I.East R.Gray D.haddock E huggard E J.R.Jones E.Montgomery I.Musgrave E Oliveira D.R.Rusby C.Spindloe B.Summers E.Zemaityte Z.zh.ng Y.Li P.McKenna D.Neely Department of Physics York Plasma InstituteUniversity of YorkHeslingtonYork YO10 5DDUK Central Laser Facility STFC Rutherford Appleton LaboratoryDidcot OX110QXUK Key Laboratory for Laser Plasmas(Ministry of Education)and School of Physics and Astronomy Shanghai Jiao Tong UniversityShanghai 200240China Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190China School of Physical Sciences University of Chinese Academy of SciencesBeijing 100049China Department of Physics SUPA University of StrathclydeGlasgow G40NGUK ENEA-C.R.Frascati-Dipartimento FSN Via E.Fermi 4500044 FrascatiItaly Space Science Department STFC Rutherford Appleton LaboratoryDidcot OX110QXUK

Giant electromagnetic pulses(EMP) generated during the interaction of high-power lasers with solid targets can seriously degrade electrical measurements and equipment. EMP emission is caused by the acceleration of hot electrons inside the target, which produce radiation across a wide band from DC to terahertz frequencies. Improved understanding and control of EMP is vital as we enter a new era of high repetition rate, high intensity lasers(e.g. the Extreme Light Infrastructure).We present recent data from the VULCAN laser facility that demonstrates how EMP can be readily and effectively reduced. Characterization of the EMP was achieved using B-dot and D-dot probes that took measurements for a range of different target and laser parameters. We demonstrate that target stalk geometry, material composition, geodesic path length and foil surface area can all play a significant role in the reduction of EMP. A combination of electromagnetic wave and 3 D particle-in-cell simulations is used to inform our conclusions about the effects of stalk geometry on EMP,providing an opportunity for comparison with existing charge separation models.

关键词： high energy density physics high-power laser related laser components laser plasmas interaction target design and fabrication

基质金属蛋白酶/金属蛋白酶组织抑制剂:基质组成中蛋白分解决定因子的变化与高血压性心脏病结构、功能和临床表现之间的关系

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世界核心医学期刊文摘（心脏病学分册） 2006年第7期2卷 40-41页

作者： Ahmed S.h. Clark L.L. Pennington W.R. M.R. Zile 刘少伟 Medical University of South Carolina Cardiology-/Medicine 135 Rutledge Ave Ste 1201 Charleston SC 29425 United States Dr.

Background -Chronic hypertension may cause left ventricular(LV) remodeling, alterations in cardiac function, and the development of chronic heart failure(ChF). Changes in the composition of the extracellular matrix(ECM) known to occur in hypertension are believed to be causally related to these structural, functional, and clinical outcomes. however, whether the determinants of ECM composition, such as the balance between ECM proteases(matrix metalloproteinases[MMPs]) and their tissue inhibitors(TIMPs), are altered in hypertensive heart disease is unknown. Methods and Results -Plasma MMP-2,-9, and -13 values, TIMP-1 and-2 values, and Doppler echocardiography images were obtained for 103 subjects divided into 4 groups:(1) reference subjects(CTL) with no evidence of cardiovascular disease,(2) hypertensive(hTN)subjects with controlled blood pressure and no LV hypertrophy,(3) hypertensive subjects with controlled blood pressure and with LV hypertrophy(hTN+LVh) but no ChF, and(4) hypertensive subjects with controlled blood pressure, LVh, and ChF(hTN+LVh+ChF). Compared with CTL, patients with hTN had no significant changes in any MMP or TIMP. Patients with hTN+LVh had decreased MMP-2 and MMP-13 values and increased MMP-9 values. Only patients with hTN+LVh+ChF had increased TIMP-1 values. A TIMP-1 level >1200 ng/mL was predictive of *** -Patients with hypertension but normal LV structure and function had normal MMP/TIMP profiles. Changes in MMP profiles that favor decreased ECM degradation were associated with LVh and diastolic dysfunction. An increased TIMP-1 level predicted the presence of ChF. Although these findings should be confirmed in a larger prospective study, these data do suggest that changes in the MMP/TIMP balance may play an important role in the structural, functional, and clinical manifestations of hypertensive heart disease.

关键词：基质金属蛋白酶组织抑制剂高血压性基质组成蛋白分解决定因子细胞外基质慢性心力衰竭心血管

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伴有和不伴有脑梗死的阿尔茨海默病

世界核心医学期刊文摘（神经病学分册） 2005年第8期1卷 44-45页

作者： Del Ser T. hachinski V. Merskey h. Munoz D.G. 张玉龙 Sección de Neurologa Hospital Severo Ochoa Avda.Orellana s/n Leganés 28911 Madrid Spain

Objective: To compare the clinical and pathological features of Alzheimer’s d isease (AD) patients with and without associated cerebral infarcts (CI). Methods : The consecutive records of 57 prospectively studied demented patients fulfilli ng the CERAD criteria for the pathological diagnosis of AD were *** w ith cortical Lewy bodies were excluded. CI were found in 22 cases (39%) (AD+CI group): large infarcts (5), lacunes(13) and/or hip pocampal sclerosis (4), and were absent in 35cases (AD group). Microscopic inf arcts, cribiform change,amyloid angiopathy, and white matter rarefaction were no t considered in this classification, but were quantified. Corticalatrophy, neuro fibrillary tangle and senile plaque (diffuse and neuritic) load were also measur ed. Pathological evaluation was independent of clinical information. Clinical an d pathological data were compared between both groups. Results: AD+CI cases wer e significantly older, more commonly female, less educated, and more often had b lue collar occupations, sleep disturbances, frontal release signs, and EEG spike s than AD cases. Other differences found (acute/subacute onset, behavioral distu rbances, and leukoaraiosis on CT scan) disappeared after controlling for age. Th e frequency of known vascular risk factors and focal motor and sensory signs did not differ between the groups, which showed remarkable clinical similarity over all. The only significant differences on pathological exam were hippocampal micr oinfarcts and white matter lesions,although there was a trend for lower neurodeg enerative lesion load in the AD+CI group. The ischemic lesions were located in temporal lobe in 50%of AD+CI patients; these cases had a significantly lower n eocortical neurodegenerative lesion load than those with CI in other sites. Conc lusions: The presence of CI in AD increases significantly with age, but has scar ce influence on the clinical features, and cannot be predicted from common vascu lar risk factors.

关键词：阿尔茨海默病神经纤维缠结淀粉样血管病血管危险因素皮质萎缩大面积梗死白质病变神经变性海马硬化脑白质疏松症

局部手张力障碍的抓握力量失常是全身感觉运动整合功能损伤的证据

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世界核心医学期刊文摘（神经病学分册） 2005年第10期1卷 26-27页

作者： Nowak D.A. Rosenkranz K. Topka h. Rothwell J. 李一明 Abteilung fr Neurologie und Klinische Neurophysiologie Stdtisches Krankenhaus Bogenhausen Lehrkrankenhaus der Technischen Universitt MnchenEnglschalkingerstrafle 77 D- 81925 Mnchen Germany Dr.

Background: Focal task specific dystonia occurs preferentially during perform ance of a specific task. There may be an inefficiently high grip force when doin g manipulative tasks other than the trigger task, possibly reflecting a generali sed impairment of sensory- motor integration. Objective: To examine how well su bjects with writer’ s cramp (n=4) or musician’ s cramp (n=5) adapted their gri p force when lifting a new object or catching a weight. Methods: Nine patients with focal hand dystonia and 10 controls were studied. Experiments addressed different motor behaviours: (A) lifting and holding an ob ject; (B) adjusting grip force in anticipation of or in reaction to a change in load force by catching a small weight dropped expectedly or unexpectedly into a hand held receptacle. Results: In (A), patients produced a grip force overshoot during the initial lifts; force overflow was most pronounced in those with write r’ s cramp. Patients and controls adjusted their grip force to object weight wi thin one or two lifts, though patients settled to a steady force level above nor mal. In (B), patients with focal hand dystonia and normal controls showed simila r predictive grip force adjustments to expected changes in object load, suggesti ng that this aspect of sensory- motor integration was normal. Patients had a sh orter latency of grip force response than controls after an unexpected load incr ease, reflecting either a greater level of preparatory motor activity or a disin hibited spinal reflex response. Conclusions: The overall increased grip force in patients with focal hand dystonia is likely to be a prelearned phenomenon rathe r than a primary disorder of sensory- motor integration.

关键词：张力障碍感觉运动抓握功能损伤书写痉挛运动行为任务性反射亢进

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苏木提取液抑制肿瘤作用的研究

肿瘤研究与临床 2006年第11期18卷 726-727页

作者：徐建国郭素堂乔丽娟张少云史天良刘维兰郑启英山西省肿瘤研究所药理生物室太原030013

目的探讨中药苏木(CAE)体内外抗肿瘤作用。方法体内采用小鼠h22腹水瘤模型评价CAE提取液的疗效。体外采用流式细胞技术,测定CAE提取液对K562细胞的杀伤作用。结果CAE提取液对小鼠肝癌h22腹水瘤在静脉和腹腔2种途径给药,均能显著延长小... 详细信息

目的探讨中药苏木(CAE)体内外抗肿瘤作用。方法体内采用小鼠h22腹水瘤模型评价CAE提取液的疗效。体外采用流式细胞技术,测定CAE提取液对K562细胞的杀伤作用。结果CAE提取液对小鼠肝癌h22腹水瘤在静脉和腹腔2种途径给药,均能显著延长小鼠的生存时间。腹腔组达到193.4%;静脉组达到150.9%。体外对K562细胞也呈明显的杀伤作用。结论CAE提取液具有抑制和杀死癌细胞的作用。

关键词：苏木提取液抑制肿瘤作用

免疫性血小板减少患者血小板糖蛋白Ⅱb/Ⅲa的表达及意义

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中国药物与临床 2010年第2期10卷 131-133页

作者：常丽贤杨林花陈剑芳张丽刘秀娥任方刚刘俊庆山西医科大学第二医院血液科太原030001

目的检测免疫性血小板减少性紫癜(ITP)患者血小板表面糖蛋白(GP)Ⅱb/Ⅲa的表达情况并探讨其临床意义。方法应用流式细胞术检测ITP、非免疫性血小板减少患者及健康对照血小板表面GPⅡb/Ⅲa的阳性表达率。结果ITP、非免疫性血小板减少患... 详细信息

目的检测免疫性血小板减少性紫癜(ITP)患者血小板表面糖蛋白(GP)Ⅱb/Ⅲa的表达情况并探讨其临床意义。方法应用流式细胞术检测ITP、非免疫性血小板减少患者及健康对照血小板表面GPⅡb/Ⅲa的阳性表达率。结果ITP、非免疫性血小板减少患者血小板数较健康对照明显减少(P0.05);ITP、非免疫性血小板减少患者血小板表面GPⅡb/Ⅲa的阳性表达率较健康对照明显减少(P<0.01),ITP较非免疫性血小板减少患者的血小板GPⅡb/Ⅲa低,且差异有统计学意义(P<0.01)。结论流式细胞术检测血小板GPⅡb/Ⅲa对ITP的诊断、鉴别诊断指导用药有一定意义。

关键词：紫癜,血小板减少血小板糖蛋白GPⅡb/Ⅲa复合物流式细胞术

电纺纳米纤维用于环境空气中多环芳烃的采样及测定

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公共卫生与预防医学 2018年第1期29卷 26-29页

作者：王华明黄文耀聂晓明孔芳陈颖郑孝杰李永刚唐琳闻胜湖北省疾病预防控制中心卫生检验检测研究所应用毒理湖北省重点实验室武汉430079

目的发展基于电纺纳米纤维(NFs)的环境空气中15种多环芳烃(PAhs)的采样及测定方法。方法利用静电纺丝制备聚苯乙烯-co-马来酸NFs,用于环境空气中PAhs的采集。NFs于丙酮中超声溶解,释放所采集的PAhs,实现快速的样品提取。样品经浓缩净化... 详细信息

目的发展基于电纺纳米纤维(NFs)的环境空气中15种多环芳烃(PAhs)的采样及测定方法。方法利用静电纺丝制备聚苯乙烯-co-马来酸NFs,用于环境空气中PAhs的采集。NFs于丙酮中超声溶解,释放所采集的PAhs,实现快速的样品提取。样品经浓缩净化,以高效液相色谱-荧光检测器(hPLC)进行检测。结果 NFs可同时实现大气颗粒物和气相中PAhs的采集,并将提取过程由索氏提取16 h缩短至超声30 min。方法对环境空气中15种PAhs检测的线性相关系数0.999 2~1.000 0,检出限0.01ng/m3~0.20ng/m3,相对标准偏差1.09%~6.57%,加标回收率84.94%~110.21%。环境空气中PAhs的采样及检测结果与环保部标准方法 hJ 647-2013一致。结论方法极大地简化了PAhs的采样及提取过程,检测灵敏度、精密度、准确度良好,能够良好满足环境空气中PAhs采样及检测的需求。研究拓展了电纺纳米纤维在环境污染物分析领域的应用。

关键词：电纺纳米纤维多环芳烃高效液相色谱采样环境空气