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卵巢内瘦素受体缺失对卵巢生殖功能和全身糖脂代谢的影响

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科技导报 2008年第19期26卷 84-89页

作者：曲军卫张跃辉吴效科侯丽辉 r.ErKKOLA J.F.strauss 匡海学南京大学医学院南京210009 黑龙江中医药大学附属第一医院妇产科哈尔滨150040 芬兰图尔库大学中心医院妇产科美国弗吉尼亚邦联大学医学院黑龙江中医药大学中药学院哈尔滨150040

为探索卵巢内瘦素信号传导缺失对卵巢自身功能和全身糖脂代谢的影响,阐明瘦素对外周生殖系统的直接作用,进行了以下研究:①对瘦素长受体缺失(Lr-)的雌性***-m+/+Leprdb小鼠和其同窝出生的有完整Lr的雌性野生型C57BL/6J小鼠(Lr+),在性成... 详细信息

为探索卵巢内瘦素信号传导缺失对卵巢自身功能和全身糖脂代谢的影响,阐明瘦素对外周生殖系统的直接作用,进行了以下研究:①对瘦素长受体缺失(Lr-)的雌性***-m+/+Leprdb小鼠和其同窝出生的有完整Lr的雌性野生型C57BL/6J小鼠(Lr+),在性成熟期后的12周,进行卵巢相互移植,构建实验小鼠的Lr基因型组合分别为A(躯体+,卵巢-)、B(躯体+,卵巢-)、C(躯体+,卵巢+)、D(躯体-,卵巢+)、E(躯体-,卵巢-)共5组(n=5),移植后观测2个动情周期取材;②检测各自的全身糖脂代谢指标、卵巢周期和生殖激素;③卵泡刺激素(0.75IU/g)刺激试验后,用免疫组化和蛋白质印迹(WB)方法,检测卵巢内脂代谢信号蛋白包括Janus激酶(JAK2)、磷酸化的丝裂原激活蛋白激酶(p-ErK)和低密度脂蛋白受体(LDLr)的表达。结果显示:①D、E组与A、B、C3组比较,体重相差约1倍,性腺周围脂肪垫重量相差约10倍(Ph、Lh水平显著下降(Ph刺激后p-ErK和LDLr在A、B、C3组卵巢内均呈强阳性表达,而D和E组则表达下降。进而得出结论:①单纯卵巢内瘦素信号缺失并不影响卵巢自身的生殖功能、脂质代谢和全身的糖脂代谢。②瘦素受体全身缺失小鼠的卵巢功能障碍,与卵巢外的全身因素即高糖脂血症和低促性腺激素状态有关。③高糖脂血症降调节卵巢内的瘦素信号蛋白JAK2的表达,诱导小鼠卵巢脂质化和功能障碍;FSh刺激可诱导ErK磷酸化后LDLr的表达增强,缓解小鼠卵巢的脂质化和功能障碍。

关键词：卵巢多囊卵巢综合征瘦素信号传导

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肠系膜-脾-门血管的动脉数字减影血管造影

放射学实践 1986年第2期 42-45页

作者： Von h. P. Busch J. hoevels P. Prager L. strauss 王家(马龙) 同济医科大学附属同济医院

近年来,数字减影血管造影(DSA)使放射学检查范围有了很大扩展。有关静脉DSA的应用已有不少报道,近来又渐积累了动脉DSA的经验。本文探讨动脉DSA显示内脏血管的价值。材料和方法动脉DSA的指征是,术前了解门静脉高

关键词：腹腔动脉 DSA 常规肠系膜腹膜数字减影血管造影血管

Overexpression of ShOrT VEGETATIVE PhASE-LIKE (SVL) in Populus delays onset and reduces abundance of flowering in field-grown trees

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horticulture research 2021年第1期8卷 2404-2415页

作者： Greg S.Goralogia Glenn T.howe Amy M.Brunner Emily helliwell Michael F.Nagle Cathleen Ma haiwei Lu Amanda L.Goddard Anna C.Magnuson Amy L.Klocko Steven h.strauss Department of Forest Ecosystems and Society Oregon State UniversityCorvallisORUSA Department of Forest resources and Environmental Conservation Virginia TechBlacksburgVAUSA Department of Biology University of Colorado Colorado SpringsColorado SpringsCOUSA

The spread of transgenes and exotic germplasm from planted crops into wild or feral species is a difficult problem for public and regulatory acceptance of genetically engineered plants,particularly for wind-pollinated trees such as *** report that overexpression of a poplar homolog of the floral repressor ShOrT VEGETATIVE PhASE-LIKE(SVL),a homolog of the Arabidopsis MADS-box repressor ShOrT VEGETATIVE PhASE(SVP),delayed the onset of flowering several years in three genotypes of field-grown transgenic *** expression of SVL correlated with a delay in flowering onset and lower floral abundance,and did not cause morphologically obvious or statistically significant effects on leaf characteristics,tree form,or stem *** effects on reproductive and vegetative phenology in spring was modest and *** results suggest that use of SVL and related floral repressors can be useful tools to enable a high level of containment for vegetatively propagated short-rotation woody energy or pulp crops.

关键词： field planted crops

Methane Concentration Influence on Combustion in a rapid Compression Machine under DFICE relevant Conditions

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Journal of Mechanics Engineering and Automation 2023年第2期13卷 40-53页

作者： r.Clemente-Mallada A.Peter S.riess L.strauss h.Fajri M.Wensing Fluid Systems Engineering Friedrich-Alexander UniversityCauerstrasse 4Erlangen-Tennenlohe 91058Germany

In this experimental work,an optically accessible rapid compression machine is used to study the ignition and combustion process under engine relevant operation conditions for five different air-natural gas equivalence ratios(λ)ignited with either 12.2 mg or 6.7 mg of pilot diesel injected at 1,600 *** temperature of the ambient mixture,walls and injector was 333 ***,for the short(6.7 mg)diesel injection,the variation in the ID(ignition delay)for two higher ambient temperatures(343 K and 353 K)was *** and piston displacement are recorded while two high-speed cameras simultaneously capture signals in the visible range spectrum and at 305 nm wavelength for Oh^(*)chemiluminescence *** is measured both from Oh^(*)and pressure *** the recorded data,the heat release ratio is estimated and compared with the visual *** gives an insight of the temporal and spatial evolution of the flame,as well as a qualitative perception of the transition from spray ignition into a premixed flame in the ambient fuel-air *** was found that increasing the methane concertation delays the ignition,reduces the natural flame luminosity and enhances the Oh^(*)chemiluminescence signal.

关键词： Dual-fuel natural gas diesel rapid compression machine ID.

robust high-Throughput Phenotyping with Deep Segmentation Enabled by a Web-Based Annotator

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Plant Phenomics 2022年第1期4卷 281-297页

作者： Jialin Yuan Damanpreet Kaur Zheng Zhou Michael Nagle Nicholas George Kiddle Nihar ADoshi Ali Behnoudfar Ekaterina Peremyslova Cathleen MaSteven H.StraussFuxin Li 11 Oregon State UniversityCorvallisORUSA University of Southern California Los AngelesCAUSA

The abilities of plant biologists and breeders to characterize the genetic basis of physiological traits are limited by their abilities to obtain quantitative data representing precise details of trait variation,and particularly to collect this data at a high-throughput scale with low *** deep learning methods have demonstrated unprecedented potential to automate plant phenotyping,these methods commonly rely on large training sets that can be time-consuming to generate.

关键词： Deep Web details

酸中毒时心脏克隆钾离子通道Kv1．4的动力学特性

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国际心血管病杂志 2007年第1期34卷 63-65页

作者：田莉莉王世敏蒋学俊李晓艳黄从新杨波 harold C.strauss 武汉大学人民医院心血管内科 430060 New Yok State University at Buffalo

目的：探讨心脏克隆钾离子通道Kv1．4的C型失活(Kv1．4△N)在非洲蟾蜍卵母细胞上表达后的动力学特性以及酸中毒时的改变。方法：将Kv1．4△N crNA(最大体积为50 n1)注入非洲爪蟾的卵母细胞内,于18℃孵育16h以上。电极采用两步法拉制,微... 详细信息

目的：探讨心脏克隆钾离子通道Kv1．4的C型失活(Kv1．4△N)在非洲蟾蜍卵母细胞上表达后的动力学特性以及酸中毒时的改变。方法：将Kv1．4△N crNA(最大体积为50 n1)注入非洲爪蟾的卵母细胞内,于18℃孵育16h以上。电极采用两步法拉制,微电极由1．5 mm口径的电极拉制。电极内充3M KCl,电阻0．5～1．0MΩ。采用双微电极电压钳制法(two electrode voltage clamp,TEV)在室温下(20～24℃)记录电流。结果：与正常ph时相比,酸性环境下,Kv1．4△N的峰电流减小,在ph7．4时通道在去极化至-40mv激活,而ph 6．8时为-30mv激活；通道在ph 7．4时最大失活为0．384±0．072,而在ph6．8时为0．197±0．013；在ph6．8时通道复活减慢(P＜0．05)。结论：酸中毒导致通道电流减小,并且使通道失活加快和恢复减慢。

关键词：卵母细胞克隆钾离子通道 Kv1.4 酸中毒

分子吸附再循环系统对肝性脑病患者动脉氨基酸水平及大脑氨基酸代谢的影响

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世界核心医学期刊文摘（胃肠病学分册） 2005年第3期1卷 61-62页

作者： Schmidt L.E. Tofteng F. strauss G.I. Larsen F.S. 程欣 Dept. of hepatology A. 2.12.1 Rigshospitalet Blegdamsvej 9 DK-2100 CopenhagenФ Denmark

Background: Liver failure is associated with low concentrations of branched chain amino acids and high concentrations of most other amino acids. In this study the effect of treatment with the Molecular Adsorbents recirculating System (MArS) on arterial amino acid levels and cerebral amino acid metabolism was examined in patients with severe hepatic encephalopathy. Methods: The study included seven patients with hepatic encephalopathy from fulminant hepatic failure (FhF) and five patients with hepatic encephalopathy from acute on chronic liver failure (AoCLF). Cerebral blood flow and cerebral arteriovenous differences in amino acids were measured before and after 6 h of treatment with MArS. results: During MArS treatment, the total arterial amino acid concentration decreased by 20%from 8.92 ±7.79 mmol/L to 7.16 ±5.64 mmol/L (P he concentration decreased in all amino acids with the exception of the branched chain amino acids. Fischer’s ratio of branched chain to aromatic amino acids increased from 0.73 ±0.47 to 0.91 ±0.54 (P rebral efflux of amino acids in patients with FhF (8.94 ±8.34 μmol;/100 g/min) as well as AoCLF (7.35 ±24.97 μmol/100 g/min) was not affected by the MArS treatment. MArS had no effect on the cerebral metabolic rate of any single amino acid in either group. Conclusions: MArS treatment tends to normalize the arterial amino acid concentrations in patients with hepatic encephalopathy. Even though the overall reduction in plasma amino acids and improvement in amino acid dysbalance may well be beneficial, it was not accompanied by any immediate improvement in cerebral amino acid metabolism in patients with FhF or AoCLF.

关键词：肝性脑病氨基酸水平氨基酸代谢暴发性肝衰竭支链氨基酸血流量慢性肝衰竭动静脉急性发作 Fischer

Effect of replacement of soybean oil by hermetia illucens fat on performance,digestibility,cecal microbiome,liver transcriptome and liver and plasma lipidomes of broilers

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Journal of Animal Science and Biotechnology 2023年第4期14卷 1466-1484页

作者： Lea Schäfer Sarah M.Grundmann Garima Maheshwari Marcus höring Gerhard Liebisch Erika Most Klaus Eder robert ringseis Institute of Animal Nutrition and Nutrition Physiology Justus Liebig University GiessenHeinrich-Buff-Ring 26-3235392 GiessenGermany Institute of Nutritional and Food Sciences Molecular Food TechnologyUniversity of BonnFriedrich-Hirzebruch-Allee 753115 BonnGermany Institute of Clinical Chemistry and Laboratory Medicine University Hospital of RegensburgFranz-Josef-Strauss-Allee 1193053 RegensburgGermany.

Background In contrast to protein-rich insect meal,the feed potential of insect fat is generally less explored and knowledge about the suitability of insect fat as a fat source specifically in broiler diets is still *** view of this,the present study aimed to comprehensively investigate the effect of partial(50%) and complete replacement of soybean oil with insect fat from hermetia illucens(hI) larvae in broiler diets on performance,fat digestibility,cecal microbiome,liver transcriptome and liver and plasma ***,100 male,1-day-old Cobb 500 broilers were randomly assigned to three groups and fed three different diets with either 0(group hI-0,n cens(hI) larvae fat for 35 d.= 30),2.5%(group hI-2.5,n %(hI-5.0,n = 35) or 5.0= 35) hermetia illuresults Body weight gain,final body weight,feed intake,and feed:gain ratio during the whole period and apparent ileal digestibility coefficient for ether extract were not different between *** microbial diversity did not differ between groups and taxonomic analysis revealed differences in the abundance of only four low-abundance bacterial taxa among groups;the abundances of phylum Actinobacteriota,class Coriobacteriia,order Coriobacteriales and family Eggerthellaceae were lower in group hI-5.0 compared to group hI-2.5(P rations of total and individual short-chain fatty acids in the cecal digesta were not different between the three *** transcriptomics revealed a total of 55 and 25 transcripts to be differentially expressed between groups hI-5.0 ***-0 and groups hI-2.5 ***-0,respectively(P he concentrations of most lipid classes,with the exception of phosphatidylethanolamine,phosphatidylglycerol and lysophosphatidylcholine in the liver and cholesterylester and ceramide in plasma(P he sum of all lipid classes were not different between *** Partial and complete replacement of soybean oil with hI larvae fat in broiler diets had no effect on gro

关键词： Broilers Cecal microbiota hermetia illucens Insect fat Liver lipidome Medium-chain fatty acids

Comprehensive evaluation of the metabolic effects of insect meal from Tenebrio molitor *** growing pigs by transcriptomics, metabolomics and lipidomics

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Journal of Animal Science and Biotechnology 2020年第2期11卷 527-544页

作者： Sandra Meyer Denise K.Gessner Maria S.Braune Theresa Friedhoff Erika Most Marcus höring Gerhard Liebisch holger Zorn Klaus Eder robert ringseis Institute of Animal Nutrition and Nutrition Physiology Justus Liebig University GiessenHeinrich-Buff-Ring 26-3235392 GiessenGiessenGermany Institute of Clinical Chemistry and Laboratory Medicine University Hospital of RegensburgFranz-Josef-Strauss-Allee 1193053 RegensburgGermany Institute of Food Chemistry and Food Biotechnology Justus Liebig University GiessenHeinrich-Buff-Ring 1735392 GiessenGiessenGermany Fraunhofer Institute for Molecular Biology and Applied Ecology Winchester Str.235394 GiessenGiessenGermany

Background:The hypothesis was tested that insect meal(IM)as protein source influences intermediary metabolism of growing *** test this,30 male,5-week-old crossbred pigs were randomly assigned to 3 groups of 10 pigs each with similar body weights(BW)and fed isonitrogenous diets either without(CON)or with 5%IM(IM5)or 10%IM(IM10)from Tenebrio molitor *** 4 weeks and key metabolic tissues(liver,muscle,plasma)were analyzed using ***:Most performance parameters did not differ across the groups,whereas ileal digestibilities of most amino acids were 6.7 to 15.6%-units lower in IM10 than in CON(Pranscriptomics of liver and skeletal muscle revealed a total of 166 and 198,respectively,transcripts differentially expressed between IM10 and CON(Prevealed higher concentrations of alanine,citrulline,glutamate,proline,serine,tyrosine and valine and a lower concentration of asparagine in IM10 than in CON(Prteen quantifiable amino acid metabolites,namely methionine sulfoxide(MetS),in plasma was elevated by 45%and 71%in IM5 and IM10,respectively,compared to CON(Prations of both,major carnitine/acylcarnitine species and bile acids were not different across *** of liver and plasma demonstrated no differences in the concentrations of triacylglycerols,cholesterol and the main phospholipids,lysophospholipids and sphingolipids between *** percentages of all individual phosphatidylcholine(PC)and phosphatidylethanolamine(PE)species in the liver showed no differences between groups,except those with 6 double bonds(PC 38:6,PC 40:6,PE 38:6,PE 40:6),which were markedly lower in IM10 than in CON(Ph this,the percentage of C22:6n-3 in hepatic total lipids was lower in IM10 than in the other groups(Prehensive analyzes of the transcriptome,lipidome and metabolome of key metabolic tissues indicate that partial or complete replacement o

关键词： Animal nutrition Insect meal Lipidome Liver Metabolism Metabolome Pigs Plasma Skeletal muscle Transcriptome