检索结果-南通市图书馆

Interleukin-6 secretion is limited by self-signaling in endosomes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2019年第2期11卷 144-157页

作者： Danielle R.J.Verboogen Natalia h.Revelo martin ter Beest Geert van den Bogaart Departme nt of Tumor Immuno logy Radboud Institute for Molecular Life Sciences Radboud University Medical Center 6525 GA Nijmegen The Netherlands Department of Molecular Immunology Groningen Biomolecular Sciences and Biotechnology Institute University of Groningen Nijenborgh 7 9747 AG Groningen The Netherlands

Cells producing cytokines often express the receptor for the same cytokine, which makes them prone to autocrine signaling. how cytokine release and signaling are regulated in the same cell is not understood? In this study, we demonstrate that signaling by exogenous and self-synthesized inflammatory cytokine interleukin-6 (IL-6) within endosomal compartments acts as a cellular brake that limits the synthesis of IL-6. Our data show that IL-6 is internalized by dendritic cells and signals from endosomal compartments containing the IL-6 receptor. Newly synthesized IL-6 also traffics via these endosomal compartments and signals in transit to the plasma membrane. This allows activation of STAT3 which in turn limits toll-like receptor 4 stimulant lipopolysaccharide (LPS) triggered transcription of IL-6. Long-term exposure to LPS removes this brake via inhibition of STAT3 by increased expression of suppressor of cytokine signaling 3 and results in fully fledged IL-6 production. This transient regulation could prevent excessive IL-6 production during early infections.

关键词： membrane trafficking cytokine release exocytosis interleukin-6 endosomal signaling

Trading in your spindles for blebs： the amoeboid tumor cell phenotype in prostate cancer

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2014年第4期16卷 530-535页

作者： Samantha Morley martin h hager Sara G Poilan Beatrice Knudsen Dolores Di Vizio Michael R Freeman Division of Cancer Biology and Therapeutics Departments of Surgery Medicine and Biomedical Sciences and The Samuel Oschin Comprehensive Cancer Institute Cedars-Sinai Medical Center Los Angeles CA Urological Diseases Research Center Boston Children's Hospital Boston MA Department of Surgery Harvard Medical School Boston MA Department of Medicine David Geffen School of Medicine University of California Los Angeles CA USA.

Prostate cancer （PCa） remains a principal cause ofmortalityin developed *** no clinical interventions overcome resistance to androgen ablation therapy, management of castration resistance and metastatic disease remains largely untreatable. Metastasis is a multistep process in which tumor cells lose cell-cell contacts, egress from the primary tumor, intravasate, survive shear stress within the vasculature and extravasate into tissues to colonize ectopic sites. Tumor ceils reestablish migratory behaviors employed during nonneoplastic processes such as embryonic development, leukocyte trafficking and wound healing. While mesenchymal motility is an established paradigm of dissemination, an alternate, ＇amoeboid＇ phenotype is increasingly appreciated as relevant to human cancer.

关键词： SIGNALING COOPERATE RhO GTPASES INVASION MOTILITY MIGRATION TRANSITION PLASTICITY ROCK METASTASIS ACTIVATION

The ph of the Apoplast： Dynamic Factor with =unctional Impact Under Stress

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2017年第11期10卷 1371-1386页

作者： Christoph.martin Geilfus Division of Controlled Environment horticulture Faculty of Life Sciences Albrecht Daniel Thaer-Institute of Agricultural and Horticultural Sciences Humboldt-University of Berlin Albrecht-Thaer-Weg 1 14195 Berlin Germany

The apoplast is an interconnected compartment with a thin water-film that alkalinizes under stress. This systemic ph increase may be a secondary effect without functional implications, arising from ion movements or proton-pump regulations. On the other hand, there are increasing indications that it is part of a mechanism to withstand stress. Regardless of this controversy, alkalinization of the apoplast has received little attention. The apoplastic ph （Phapo） increases not only during plant-pathogen interactions but also in response to salinity or drought. Not much is known about the mechanisms that cause the leaf apoplast to alkalinize, nor whether, and if so, how functional impact is conveyed. Controversial explanations have been given, and the unusual complexity of phapo regulation is considered as the primary reason behind this lack of knowledge. A gathering of scattered information revealed that changes in Phapo convey functionality by regulating stomatal aperture via the effects exerted on abscisic acid. Moreover, apoplastic alkalinization may regulate growth under stress, whereas this needs to be verified. In this review, a comprehensive survey about several physiological mechanisms that alkalize the apoplast under stress is given, and the suitability of apoplastic alkalinization as transducing element for the transmission of sensory information is discussed.

关键词： apoplastic fluid alkalinization abscisic acid PM-h^+-ATPase S-Type anion channel malate

Sainfoin(Onobrychis viciifolia)silage in dairy cow rations reduces ruminal biohydrogenation and increases transfer efficiencies of unsaturated fatty acids from feed to milk

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Nutrition 2020年第3期6卷 333-341页

作者： Nguyen Thi huyen martin W.A.Verstegen Wouter h.hendriks Wilbert F.Pellikaan Animal Nutrition Group Wageningen UniversityPO Box 338Wageningen6700 AHthe Netherlands Department of Farm Animal health Utrecht UniversityPO Box 80.163Utrecht3508 TDthe Netherlands

The effects of replacing grass silage by sainfoin silage in a total mixed ration(TMR)based diet on fatty acid(FA)reticular inflow and milk FA profile of dairy cows was *** experiment followed a crossover design with 2 dietary *** control diet consisted of grass silage,corn silage,concentrate and *** the sainfoin diet,half of the grass silage was replaced by a sainfoin *** rumen cannulated lactating multiparous dairy cows with a metabolic body weight of 132.5±3.6 kg BW^0.75,214±72 d in milk and an average milk production of 23.1±2.8 kg/d were used in the *** were paired based on parity and milk *** pairs,cows were randomly assigned to either the control diet or the sainfoin diet for 2 experimental periods(29 d per period).In each period,the first 21 d,cows were housed individually in tie-stalls for adaptation,then next 4 d cows were housed individually in climate-controlled respiration chambers to measure *** the last 4 d,cows were housed individually in tie stalls to measure milk FA profile and determine FA reticular inflow using the reticular sampling technique with Cr-ethylenediaminetetraacetic acid disodium salt dihydrate(EDTA)and Yb-acetate used as digesta flow *** the dietary C18:3n-3 intake was lower(P=0.025)in the sainfoin diet group,the mono-unsaturated FA reticular inflow was greater(P=0.042)in cows fed the sainfoin *** reticular inflow of trans-9,trans-12-C18:2 and cis-12,trans-10 C18:2 was greater(P≤0.024)in the sainfoin diet *** cows fed sainfoin diet had a lower(P≤0.038)apparent ruminal biohydrogenation of cis-9-C18:1 and C18:3n-3,compared to the cows fed the control *** sainfoin diet group had greater(P≤0.018)C18:3n-3 and cis-9,cis-12-C18:2 proportions in the milk FA profile compared to the control diet *** efficiencies from feed to milk of C18:2,C18:3n-3 and unsaturated FA were greater(P≤0.0179)for the sainfoin *** on the results,it co

关键词： Sainfoin silage Reticular inflow Milk fatty acid profile Dairy cow

Therapeutic cell engineering:designing programmable synthetic genetic circuits in mammalian cells

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Protein & Cell 2022年第7期13卷 476-489页

作者： Maysam Mansouri martin Fussenegger Department of Biosystems Science and Engineering ETH ZurichMattenstrasse 264058 BaselSwitzerlandDepartment of Biosystems Science and EngineeringETH ZurichMattenstrasse 264058 BaselSwitzerland Faculty of Science University of BaselMattenstrasse 264058 BaselSwitzerland

Cell therapy approaches that employ engineered mam-malian cells for on-demand production of therapeutic agents in the patient's body are moving beyond proof-of-concept in translational *** therapeutic cells can be customized to sense user-defined signals,pro-cess them,and respond in a programmable and pre-dictable *** this paper,we introduce the available tools and strategies employed to design therapeutic ***,various approaches to control cell behav-iors,including open-loop and closed-loop systems,are *** also highlight therapeutic applications of engineered cells for early diagnosis and treatment of various diseases in the clinic and in experimental dis-ease ***,we consider emerging technolo-gies such as digital devices and their potential for incorporation into future cell-based therapies.

关键词： synthetic biology cell-based therapy cell engineering therapeutic gene expression controllable genetic circuits

Acute pancreatitis in aging animals:Loss of pancreatitis-associated protein protection?

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2012年第26期18卷 3379-3388页

作者： Sophia Fu Albert Stanek Cathy M Mueller Nefertti A Brown Chongmin huan martin h Bluth Michael E Zenilman Department of Surgery SUNY Downstate Medical SchoolBrooklynNY 11203United States Department of Pathology Wayne State UniversityDetroitMI 48202United States Department of Surgery Johns Hopkins School of Medicine and SUNY Downstate School of Public HealthBethesdaMD 20814United States

AIM:To investigate the effect of age on severity of acute pancreatitis(AP) using biochemical markers,histology and expression of the protective pancreatitisassociated proteins(PAPs).METhODS:AP was induced via intraductal injection of 4% sodium taurocholate in young and old *** and pancreata were assayed at 24 h for the parameters listed above;we also employed a novel molecular technique to assess bacterial infiltration using polymerase chain reaction to measure bacterial genomic ribosomal ***:At 24 h after induction of AP,the pancreata of older animals had less edema(mean ± SE histologic score of young vs old:3.11 ± 0.16 vs 2.50 ±-0.11,P histologic score of stromal infiltrate:3.11 ± 0.27 vs 2.00 ± 0.17,P ham vs 377% ± 4%,P histochemical *** were no differences in serum amylase and lipase activity,or tissue myeloperoxidase or monocyte chemotactic protein-1 ***,in the most-aged group,serum C-reactive protein levels were higher(young vs old:0.249 ± 0.04 mg/dL vs 2.45 ± 0.68 mg/dL,P here is depressed PAP expression related to a blunted inflammatory response in AP which is associated with worsened bacterial infiltration and higher C-reactive protein level;this may explain the more aggressive clinical course.

关键词： Acute pancreatitis Aging Rats Pancreati-tis-associated protein Molecular biology

Video Traces for Network Performance Evaluation 2007

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Laser capture microdissection and genetic analysis of carbon-labeled Kupffer cells

2007年

作者： Patrick Seeling Frank h. P. Fitzek martin Reisslein

来源：畅想之星图书光盘版（南... 评论

在线全文

畅想之星图书光盘版（南通市图书馆镜像）

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2009年第14期15卷 1708-1718页

作者： Stephan Gehring Edmond Sabo Maryann E San martin Elizabeth M Dickson Chao-Wen Cheng Stephen h Gregory Department of Medicine Rhode Island Hospital and The Warren Alpert Medical School of Brown University Providence RI 02903 United States Department of Pathology Rhode Island Hospital and The Warren Alpert Medical School of Brown University Providence RI 02903 United States zentrum fur Kinder- und Jugendmedizin der Universitaitsmedizin der Johannes Gutenberg-Universitait 55101 Mainz Germany

AIM： To develop a method of labeling and microdissecting mouse Kupffer cells within an extraordinarily short period of time using laser capture microdissection （LCM）. METhODS： Tissues are complex structures comprised of a heterogeneous population of interconnected cells. LCM offers a method of isolating a single cell type from specific regions of a tissue section. LCM is an essential approach used in conjunction with molecular analysis to study the functional interaction of cells in their native tissue environment. The process of labeling and acquiring cells by LCM prior to mRNA isolation can be elaborate, thereby subjecting the RNA to considerable degradation. Kupffer cell labeling is achieved by injecting India ink intravenously, thus circumventing the need for in vitro staining. The significance of this novel approach was validated using a cholestatic liver injury model. RESULTS： mRNA extracted from the microdissected cell population displayed marked increases in colonystimulating factor-1 receptor and Kupffer cell receptor message expression, which demonstrated Kupffer cell enrichment. Gene expression by Kupffer ceils derived from bile-duct-ligated, versus sham-operated, mice was compared. Microarray analysis revealed a significant （2.5-fold, q value 〈 10） change in 493 genes. Based on this fold-change and a standardized PubMed search, 10 genes were identified that were relevant to the ability of Kupffer cells to suppress liver injury. CONCLUSION; The methodology outlined herein provides an approach to isolating high quality RNA from Kupffer cells, without altering the tissue integrity.

关键词： Kupffer cells India ink Laser capturemicrodissection Bile duct ligation DNA microarray

Dissipation of chlorpyrifos, pendimethalin, and thiophanate-methyl pesticides in compost based-versus peat-moss based-biomixture of biobeds

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Waste Disposal and Sustainable Energy 2023年第4期5卷 461-470页

作者： Talaat N.M.El-Sebai Wafaa M.h.Zidan Alaa Marzouk Marion Divers Fabrice martin-Laurent Agricultural Microbiology Dept.Agricultural and Biology Research Institute National Research CentreCairoEgypt Laboratory of Pesticide Analysis Agriculture Research CentreCairoEgypt Laboratory of Soil Microbiology National Institute of Agricultural ResearchDijonFrance

Biobed is a smart bioremediation system used to treat point-source pesticide *** is the main component of biobeds,and pesticide dissipation is affected by its *** study aimed to compare the effectiveness of compost-based(C)versus peat-moss-based(P)biomixtures of biobeds on tested pesticide *** concentrations(25,50,and 75 mg/kg)of chlorpyrifos,pendimethalin,and thiophanate methyl were added separately and as a mix to both biomixtures C and *** data showed the effect of biomixture type on the dissipation of the tested *** chlorpyrifos,its dissipation rate in biomixture P was more rapid than that in biomixture *** result was confirmed by the mineralization kinetic experiment,since 25%of the initial ^(14)C-chlorpyrifos concentration accumulated in the form ^(14)CO_(2) in biomixture P compared to only 14%in biomixture *** addition,the chlorpyrifos dissipation rate was influenced by the initial concentration when applied individually in biomixture *** contrast,biomixture C was more effective at pendimethalin dissipation than biomixture P,since>76%of pendimethalin was dissipated in biomixture C versus 67%in biomixture P at the same incubation *** thiophanate-methyl,the abilities of both biomixtures C and P were similar and less efficient than those of the other tested *** addition of the three tested pesticides to biomixture C only had a positive effect on both chlorpyrifos and thiophanate-methyl dissipation,while pendimethalin dissipation was similar when applied separately or as a *** activity was stimulated by the addition of separately or mixed pesticides to biomixtures C and P as measured by dehydrogenase activity.

关键词： Biopurification system Pesticides Contamination Point-source Bioremediation Biomixture