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Effects of fertilization on radial growth.of Pinus pinea explored h.urly using dendrometers

Ecological Processes 2024年第1期 25-39页

作者： verónica Loewe-Mu?oz Rodrigo del Río Millar Claudia Delard Rodriguez Mónica Balzarini Ch.lean Forest Institute (INFOR) Metropolitan Office Centro Nacional de Excelencia Para la Industria de la Madera(CENAMAD) Pontificia Universidad Católica de Chile Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Universidad Nacional de Córdoba Ciudad Universitaria

Background Stone pine(Pinus pinea), a drough.-resistant species, h.s significant socio-economic benefits and increasing interest for th. establish.ent of productive plantations in several countries, especially in a climate ch.nge context. Monitoring h.urly stem diameter variations contributes to th. understanding of th. tree-growth.response to ch.nges in environmental conditions and management. By monitoring th. diameter expansion of tree stems, h.gh.resolution band dendrometers were used to study th. development of adult trees growing in a semi-arid coastal environment of central Ch.le under fertilized and non-fertilized soil conditions th.ough.th. span of a *** Sh.rt cycles(h. were few in fertilized and non-fertilized trees(6 and 4, respectively), wh.reas long cycles(> 28 h. occurred at a h.gh.r frequency in fertilized trees(16 vs 6). Most of th. circadian cycles were regular(24 ± 3 h.. Th. longest cycle duration(59 h. was observed in fertilized trees during spring. In all seasons, each.ph.se of th. circadian cycle, especially during th. stem diameter increment ph.se(P3, irreversible growth., started earlier in fertilized th.n in control trees. P3 duration was significantly longer in fertilized th.n in control trees in springtime. Th. maximum sh.inkage(P1) was observed in summer for both.treatments. Stem diameter increased faster in fer-tilized th.n in control trees th.ough.ut th. year, with.th. h.gh.st accumulation occurring in spring and th. lowest in autumn. Th. daily variability pattern sh.wed lower growth.under h.gh.temperature across *** Th.s study h.gh.igh.s th. importance of fertilization in enh.ncing stone pine diameter growth. Th.s cultural practice sh.uld be furth.r explored to contribute to th. mitigation of climate ch.nge effects in semi-arid environments.

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LIVING/CONTROLLED GRAFTING FROM POLYMER MICROSPh.RES

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Ch.nese Journal of Polymer Science 2003年第6期21卷 639-651页

作者： h.rald D. h. Stver Dept. of Ch.mistry McMaster University Hamilton ON L8S 4MI Canada

A review is given of grafting-from meth.ds using living polymerizations, with.emph.sis on grafting from polymermicrosph.res using ATRP.

关键词： Living controlled polymerization Grafting ATRP Microsph.res

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Detection of integron integrase genes on King George Island, Antarctica

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Advances in Polar Science 2015年第1期26卷 30-37页

作者： verónica Antelo h.ctor Romero Silvia Batista Unidad de Microbiología Molecular Biogem Instituto de Investigaciones Biológicas Clemente Estable (MEC) Departamento de Ecología y Evolución Facultad de Ciencias UDeLaR Montevideo C.P.11400 Uruguay

Th. presence and diversity of class 1 integrase gene （intl） sequences were evaluated by PCR using previously designed primers. Two clone libraries were constructed from DNA in sediment and microbial mat samples collected on Fildes Peninsula, King George Island, *** libraries constructed from samples collected at h.lfth.ee Point （h.） and Norma Cove （NC） contained 62 and 36 partial intl sequences, respectively. Th.se sequences clustered into 10 different groups with.〈 95% amino acid identity. Alignment of th. deduced amino acid sequences with.th.se from recognized integron-encoded integrases demonstrated th. presence of h.gh.y conserved motifs ch.racteristic of intl integrases. Th. h. library contained 42 nucleotide sequences identical to th. class 1 intl gene found in a collection of trimeth.prim-resistant （Tmpr） Antarctic Enterobacter sp. isolates, previously collected in th. same area. Th.se integrons, located on plasmids, h.d a genetic organization similar to th.t of pKOX105 from Klebsiella oxytoca. Th. 20 remaining h. and NC library sequences were similar to integrase sequences previously determined in a metagenomic analysis of environmental samples. We h.ve demonstrated th. presence of integron integrase genes in Antarctic sediment samples. About h.lf th.se genes were very similar to th. class 1 integrons found in h.man- associated microbiota, suggesting th.t th.y originated from h.man-dominated ecosystems. Th. remaining integrase genes were probably associated with.endemic bacteria.

关键词： integrase amplicon library maritime Antarctica

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Imidazolium-based ionic liquids as additives to preserve th. Enh.nced Green Fluorescent Protein fluorescent activity

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Green Ch.mical Engineering 2021年第4期2卷 412-422页

作者： Nath.lia V.veríssimo Carolina F.Saponi Timoth. M.Ryan Tamar L.Greaves Jorge F.B.Pereira Department of Engineering of Bioprocesses and Biotech.ology School of Pharmaceutical SciencesSao Paulo State University(UNESP)Rodovia Araraquara-Jaú/Km 0114800-903AraraquaraSPBrazil Sch.ol of Science College of ScienceEngineering and HealthRMIT University124 La Trobe StreetMelbourneVIC 3000Australia Sch.ol of Ph.rmaceutical Sciences Sao Paulo University(USP)Av.Prof.Lineu Prestesno.580B1605508-000Cidade de UniversitariaSao PauloSPBrazil Australian Synch.otron 800 Blackburn RoadClaytonVIC 3168Australia Univ Coimbra CIEPQPFDepartment of Chemical EngineeringRua Sílvio LimaPolo II–Pinhal de Marrocos3030-790 CoimbraPortugal

Fluorescent Proteins(FP)can be applied as biomarkers and biosensors in th. industrial and medical fields,but th.ir large-scale use,especially for new industrial applications,is limited due to th.ir low ***,th. discovery of additives capable of preserving th. activity of FP at room temperature and under stress conditions can h.lp to expand and facilitate th.ir commercial *** th.s goal,we evaluated th. application of 1-alkyl-3-meth.limidazolium ch.oride-based([C_(n)mim]Cl)ionic liquids(ILs)as additives to preserve th. activity of Enh.nced Green Fluorescent Protein(EGFP)at different storage times and under unfavorable ***[C_(n)mim]Cl solutions(at 0.100 mol L^(-1))were able to preserve EGFP fluorescence for longer th.n th. ph.sph.tesaline buffer(PBS)and NaCl solutions,increasing its fluorescence manifestation from 1 to 3 month..[C_(n)mim]Cl with.sh.rter to medium cationic alkyl ch.ins were th. most effective in preserving EGFP fluorescence.[C_(n)mim]Cl also protected EGFP activity in th. presence of th. surfactant SDS,th. acid guanidine h.droch.oride,and h.(2)O_(2).Th.refore,[C_(n)mim]Cl can be added to aqueous solutions to preserve EGFP fluorescence activity at room temperature for longer storage times and to reduce th. negative impact of denaturing agents on ***,th.re is a massive potential for th. application of ILs as additives to preserve FP in th. long-term with.ut refrigeration and under unfavorable conditions,and th.s is fundamental to enable expansion of FP in industrial and commercial applications.

关键词： Green Fluorescent Protein Protein stability Fluorescence Ionic liquids Preservatives

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Error induced by th. estimation of th. corneal power and th. effective lens position with.a rotationally asymmetric refractive multifocal intraocular lens

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International Journal of Oph.h.lmology(English.edition) 2015年第3期8卷 501-507页

作者： David P.Piero Vicente J.Camps María L.Ramón verónica Mateo Rafael J.Pérez-Cambrodí Grupo de óptica y Percepción Visual (GOPV).Department of Optics Pharmacology and Anatomy University of Alicante San Vicente del Raspeig Alicante 03690 Spain Department of Oph.h.lmology (Oftalmar) Vithas Medimar International Hospital Foundation for th. Visual Quality (FUNCAVIS: Fundación para la Calidad Visual) Alicante 03016 Spain

AIM : To evaluate th. prediction error in intraocular lens(IOL) power calculation for a rotationally asymmetric refractive multifocal IOL and th. impact on th.s error of th. optimization of th. keratometric estimation of th. corneal power and th. prediction of th. effective lens position(ELP).METh.DS: Retrospective study including a total of 25 eyes of 13 patients(age, 50 to 83y) with.previous cataract surgery with.implantation of th. Lentis Mplus LS-312 IOL(Oculentis Gmb h. Germany). In all cases, an adjusted IOL power(P IOLadj) was calculated based on Gaussian optics using a variable keratometric index value(n kadj) for th. estimation of th. corneal power(P kadj) and on a new value for ELP(ELP adj) obtained by multiple regression *** P IOLadj was compared with.th. IOL power implanted(P IOLReal) and th. value proposed by th.ee conventional formulas(h.igis, h.ffer Q and h.lladay Ⅰ).RESULTS: P IOLReal was not significantly different th.n P IOLadj and h.lladay IOL power(P >0.05). In th. Bland and Altman analysis, P IOLadj sh.wed lower mean difference(-0.07 D) and limits of agreement(of 1.47 and-1.61 D)wh.n compared to P IOLReal th.n th. IOL power value obtained with.th. h.lladay formula. Furth.rmore, ELP adj was significantly lower th.n ELP calculated with.oth.r conventional formulas(P h. anterior ch.mber depth.and P kadj. CONCLUSION: Refractive outcomes after cataract surgery with.implantation of th. multifocal IOL Lentis Mplus LS-312 can be optimized by minimizing th.keratometric error and by estimating ELP using a math.matical expression dependent on anatomical factors.

关键词： Mplus multifocalintraocularlens keratometry effective lens position intraocular lens power

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Switch.rass Bioch.r Effects on Plant Biomass and Microbial Dynamics in Two Soils from Different Regions

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Pedosph.re 2015年第3期25卷 329-342页

作者： Ch.rlene N.KELLY Francisco C.CALDERóN verónica ACOSTA-MARTíNEZ Maysoon M.MIKh. Joseph.BENJAMIN David W.RUTh.RFORD Colleen E.ROSTAD Western Carolina University Department of Geosciences and Natural Resources331 Stillwell HallCullowheeNC 28723 (USA) U.S.Geological Survey Denver CO 80225 (USA) USDA-ARS Akron CO 80720 (USA) USDA-ARS Lubbock TX 79403 (USA)

Bioch.r amendments to soils may alter soil function and fertility in various ways, including th.ough.induced ch.nges in th. microbial community. We assessed microbial activity and community composition of two distinct clayey soil types, an Aridisol from Colorado （CO） in th. U.S. Central Great Plains, and an Alfisol from Virginia （VA） in th. south.astern USA following th. application of switch.rass （Panicum virgatum） bioch.r. Th. switch.rass bioch.r was applied at four levels, 0%,0, 2.5%, 5%, and 10%, approximately equivalent to bioch.r additions of 0, 25, 50, and 100 t h.^-1, respectively, to th. soils grown with.wh.at （Triticum aestivum） in an eigh.-week growth.ch.mber experiment. We measured wh.at sh.ot biomass and nitrogen （N） content and soil nutrient availability and N mineralization rates, and ch.racterized th. microbial fatty acid meth.l ester （FAME） profiles of th. soils. Net N mineralization rates decreased in both.soils in proportion to an increase in bioch.r levels, but th. effect was more marked in th. VA soil, wh.re net N mineralization decreased from -2.1 to -38.4 mg kg^-1. Th. 10% bioch.r addition increased soil ph. electrical conductivity, Meh.ich. and bicarbonate-extractable ph.sph.rus （P）, and extractable potassium （K） in both.soil types. Th. wh.at sh.ot biomass decreased from 17.7 to 9.1 g with.incremental additions of bioch.r in th. CO soil, but no difference was noted in plants grown in th. VA soil. Th. FAME recovery assay indicated th.t th. switch.rass bioch.r addition could introduce artifacts in analysis, so th. results needed to be interpreted with.caution. Non-corrected total FAME concentrations indicated a decline by 457o and 34% with.10% bioch.r addition in th. CO and VA soils, respectively, th.ugh.th.se differences became nonsignificant wh.n th. extraction efficiency correction factor was applied. A significant decline in th. fungi：bacteria ratio was still evident upon correction in th. CO soil with.bioch.r. Switch.rass bioch.r h.d th. p

关键词： correction factor extraction efficiency fatty acid meth.l ester profile nitrogen mineralization soil microbial community soil nutrient availability wh.at

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Polarization conversion using customized subwavelength.laser-induced periodic surface structures on stainless steel

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Ph.tonics Research 2022年第9期10卷 2024-2031页

作者： MAh.OUD h.ELSh.RBAGY LUIS MIGUEL SÁNCh.Z-BREA JERÓNIMO BUENCUERPO JESÚS DEL h.YO ÁNGELA SORIA-GARCÍA verÓNICA PASTOR-VILLARRUBIA ALEJANDRO SAN-BLAS AINARA RODRÍGUEZ SANTIAGO MIGUEL OLAIZOLA JAVIER ALDA Grupo Complutense deÓptica Aplicada Departamento deÓpticaFacultad de Ciencias FıÂsicasUniversidad Complutense de MadridPlaza de las Ciencias S.N.28040 MadridSpain Ph.sics Department Faculty of ScienceMinia University61519 El-MinyaEgypt L’Institut Ph.tovoltaïque d’Île-de-France(IPVF) 18 Bd Thomas Gobert91120 PalaiseauFrance Ceit-Basque Research.and Tech.ology Alliance(BRTA) Manuel Lardizabal 1520018 Donostia/San SebastiánSpain Universidad de Navarra TecnunManuel Lardizabal 1320018 Donostia/San SebastiánSpain

Stainless steel is a basic raw material used in many *** can be customized by generating laser-induced periodic surface structure(LIPSS)as subwavelength.***,we present th. capabilities of an LIPSS on stainless steel to modify th. polarization state of th. reflected radiation at th. IR *** structures h.ve been modeled using th. finite element meth.d and fabricated by femtosecond laser *** Stokes parameters h.ve been obtained experimentally and a model for th. sh.pe h.s been used to fit th. simulated Stokes values to th. experimental *** birefringence of th. LIPSS is analyzed to explain h.w th.y modify th. polarization state of th. incoming *** find th. geometry of th. subwavelength.grating th.t makes it work as an optical retarder th.t transforms a linearly polarized ligh. into a circularly polarized *** addition,th. geometrical parameters of th. LIPSS are tuned to selectively absorb one of th. components of th. incoming ligh.,becoming a linear axial *** selecting th. geometrical parameters and orientation of th. fabricated LIPSS makes it possible to obtain an arbitrary pure polarization state wh.n illuminated by a pure linearly polarized state oriented at an azimuth.of 45°.Th. overall reflectance of th.se transformations reach.s values close to 60%with.respect to th. incident intensity,wh.ch.is th. same reflectivity obtained for non-nanostructured stainless steel flat surfaces.

关键词： steel laser polarization

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Effect of IBD sera on expression of inducible and endoth.lial nitric oxide synth.se in h.man umbilical vein endoth.lial cells

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World Journal of Gastroenterology 2006年第11期12卷 1730-1738页

作者： Károly Palatka Zoltán Serf(o|″)z(o|″) Zoltán veréb Róbert Bátori Beáta Lontay Zoltán h.rgitay Zoltán Nemes Miklós Udvardy Ferenc Erd(o|″)di István Altorjay Department of Gastroenterology University of Debrecen Nagyerdei k(o|¨)rút 98.Debrecen H-4001Hungary Department of Experimental Zoology BLRI-h.S TihanyHungary Institute of Immunology University of Debrecen Nagyerdei k(o|¨)rút 98.Debrecen H-4001 Hungary Department of Medical Ch.mistry University of Debrecen Nagyerdei k(o|¨)rút 98.Debrecen H-4001Hungary Department of Path.logy Gyula Kenezy h.spital DebrecenBartók Béla út 10.Debrecen H-4026Hungary Institute of Path.logy University of Debrecen Nagyerdei k(o|¨)rút 98.Debrecen H-4001Hungary Institute of Internal Medicine University of Debrecen Nagyerdei k(o|¨)rút 98.Debrecen H-4001Hungary

AIM： To study th. expression of endoth.lial and inducible nitric oxide synth.ses （eNOS and iNOS） and th.ir role in inflammatory bowel disease （IBD）. METh.DS： We examined th. effect of sera obtained from patients with.active Croh.＇s disease （CD） and ulcerative colitis （UC） on th. function and viability of h.man umbilical vein endoth.lial cells （h.VEC）. h.VECs were cultured for 0-48 h.in th. presence of a medium containing pooled serum of h.alth. controls, or serum from patients with.active CD or UC. Expression of eNOS and iNOS was visualized by immunofluorescence, and quantified by th. densitometry of Western blots. Proliferation activity was assessed by computerized image analyses of Ki-67 immunoreactive cells, and also tested in th. presence of th. NOS inh.bitor, 10^-4 mol/L L-NAME. Apoptosis and necrosis was examined by th. annexin-V-biotin meth.d and by propidium iodide staining, respectively. RESULTS： In h.VEC immediately after exposure to UC, serum eNOS was markedly induced, reach.ng a peak at 12 h. In contrast, a decrease in eNOS was observed after incubation with.CD sera and th. eNOS level was minimal at 20 h.compared to control （18%±16% vs 23%± 15% P〈0.01）. UC or CD serum caused a significant increase in iNOS compared to control （UC： 300%±21%; CD： 275% ± 27% vs 108% ± 14%, P〈0.01）. Apoptosis/necrosis ch.racteristics did not differ significantly in eith.r experiment. Increased proliferation activity was detected in th. presence of CD serum or after treatment with.L-NAME. Cultures sh.wed tube-like formations after 24 h.treatment with.CD serum. CONCLUSION： IBD sera evoked ch.nges in th. ratio of eNOS/iNOS, wh.reas did not influence th. viability of h.VEC. Th.se involved down-regulation of eNOS and up-regulation of iNOS simultaneously, leading to increased proliferation activity and possibly a reduced antiinflammatory protection of endoth.lial cells.

关键词： Croh.'s disease h.man umbilical vein endoth.lial cells Inflammatory bowel disease Nitric oxide synth.se Ulcerative colitis

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Ch.mical Ch.racterization of N-h.xane Extract Obtained from Lignocellulose Residual Contained in Agroindustrial Wastes

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Journal of Agricultural Ch.mistry and Environment 2018年第2期7卷 94-104页

作者： Jorge Ernesto Solá-Pérez h.go Saldarriaga-Norena Mario Murillo-Tovar Gustavo Ronderos-Lara verónica Gisela López-Martínez Centro de Investigaciones Químicas ICCBAUniversidad Autónoma del Estado de MorelosCuernavacaMéxico

Th. ch.mical composition of n-h.xane extractives from lignocellulose residual contained in different agroindustrial wastes was studied. Th. n-h.xane extract, wh.ch.accounted for 1.6% of total lignocellulose residual weigh., was analysed by 1h.NMR and gas ch.omatograph./mass spectrometry. Th. most predominant compounds identified were aliph.tic h.drocarbons (lineal alkanes, branch.d alkanes, alkenes). Additionally, terpenes, ketones, fatty alcoh.ls, fatty acids and steroids were also found togeth.r in minor amounts. Th. lipoph.lic compounds in n-h.xane extracts in th.ee lignocellulosic wastes, wh.ch.is h.gh.y valuable information for a more complete industrial utilization of th.se lignocellulosic materials.

关键词： Wh.at Straw Maguey Bagasse Sugarcane Bagasse N-h.xane Extract

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非球面人工晶状体度数计算的最优化

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国际眼科杂志 2016年第6期16卷 1001-1008页

作者： David P.Piero Vicente J.Camps María L.Ramón verónica Mateo Roberto Soto-Negro 圣维森特-德埃拉斯佩奇阿利坎特大学视光学、药理学和解剖学系光学和视觉知觉组西班牙阿利坎特03690 Vith.s Medimar国际医院眼科西班牙阿利坎特03016

目的：通过评价非球面人工晶状体（intraocular lens,IOL）屈光度的可预测性,初步开发一种计算屈光度（PIOL）的优化算法。方法：本研究纳入植入非球面IOL（LENTIS L-313,Oculentis Gmb h.65眼,并分为2组：A组8例12眼,PIOL≥23.0D;B组3... 详细信息

目的：通过评价非球面人工晶状体（intraocular lens,IOL）屈光度的可预测性,初步开发一种计算屈光度（PIOL）的优化算法。方法：本研究纳入植入非球面IOL（LENTIS L-313,Oculentis Gmb h.65眼,并分为2组：A组8例12眼,PIOL≥23.0D;B组35例53眼,PIOL〈23.0D。术后3mo进行屈光度可预测性评价。参考角膜屈光力估计所致的可变性屈光指数计算出校正的IOL度数（PIOLadj）及屈光结果,根据年龄和解剖学因素得出校正的有效晶状体位置（adjusted effective lens position,ELPadj）。结果：术后A、B两组等效球镜度数分别为-0.75~＋0.75D、-1.38~＋0.75D。A、B两组的PIOLadj和实际晶状体屈光度（PIOLReal）之间无统计学差异（P=0.64、0.82）。BlandAltman分析显示A、B两组PIOLadj和PIOLReal之间的一致性区间分别为＋1.11^-0.96D和＋1.14^-1.18D。h.ffer Q公式和h.lladay I公式计算PIOLadj和PIOL之间存在临床和统计学上的显著差异（P〈0.01）。结论：植入非球面IOL白内障手术的屈光可预测性可通过平行轴光学联合线性法则使角膜屈光力及晶状体位置相关误差最小化。

关键词：非球面人工晶状体人工晶状体屈光度计算有效晶状体位置

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