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Effect of β-carotene on selected indices of in vitro rumen fermentation in goats

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Effect of β-carotene on selected indices of in vitro rumen ...

Carbon and nitrogen mineralization in soil of leguminous trees in a degraded pasture in north.rn Rio de Janeiro, Brazil

Th. Ⅶ International Symposium on th. Nutrition of h.rbivores(ISNh.7)

作者： h.-X.Yan L.-S.Sun G.-Q.Zh.o~1 College of Animal Science and Tech.ology,Yangzh.u University Yangzh.u 225009,P.R.Ch.na

Th.ee goats fitted with.rumen fistula were used as donors ofrumen *** were incubated in vitro with.buffered tureen fluid for 24 *** levels of β-carotene(five groups:10,50,100,200, 500 mg/l)were added as five treatment groups to determine wh.th.r it affected rumen fermentation in vitro as compared with.blank samples used as ***-N concentrations with.50,100 and 200 mg β-carotene/I group were found significantly lower th.n th.t in blank samples(P1 ).Microbial protein concentrations in all β-carotene-added groups were h.gh.r th.n in blanks,wh.le th.se found in 200 and 500 mg/I groups were significantly h.gh.r(P1,respectively).Total VFAs seemed not to be affected by added β-carotene(P>0.05),alth.ugh.propionate and butyrate concentrations *** is concluded th.t β-carotene can enh.nce th. utilization of Nh.N by rumen microorganisms and th.s promote th.ir growth.in vitro.

关键词： β-carotene rumen fermentation goat in vitro

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Journal of Forestry Research 2016年第1期27卷 91-99页

作者： Danielle A. D. Nunes Emanuela F. Gama-Rodrigues Patricia A. B. barreto[ Antonio C. Gama-Rodrigue Paulo h. M. Monroe Ch.mistry Sch.ol Rio de Janeiro Federal UniversityRio de Janeiro RJ 21941-909 Brazil Soil Laboratory North Fluminense State UniversityCampos dos Goytacazes RJ 28013-602 Brazil Department of Soil and Agricultural Engineering State University of Southwest Bahia Vitoda da ConquistaBA 45031-300 Brazil

Use of legume trees can improve soil quality in degraded pastures. Th. aim of th.s study was to ch.rac- terize C and N mineralization kinetics and estimate th. potentially mineralizable C and N in soil under Mimosa caesalpiniifolia Benth. and Acacia auriculiformis A. Cunn. ex Benth. secondary forest and pasture in red-yellow latosols in south.ast Brazil. We conducted a laboratory aerobic incubation experiment using a completely ran- domized design of four replicates and four types of plant cover using a modified version of th. Stanford and Smith.tech.ique （1972） to study C and N mineralization potential. Potentially mineralizable N （No） ranged from 135 to 170 mg kg-1. Th. predominant form of mineral N for all types of plant cover was N-NO3-. M. caesalpiniifolia was th. only species th.t h.d a positive influence on N min- eralization. Neith.r of th. legumes influenced C mineral- ization in pasture or secondary forest. Th. model of N mineralization corresponded to a sigmoidal curve wh.le C mineralization corresponded to an exponential curve, revealing th.t th. N and C mineralization processes were distinct. N mineralized by M. caesalpiniifolia （216 kg ofN h.-1） was adequate to meet th. N requirement for a livestock-forest system.

关键词： Carbon dioxide Forest soil Mineralized N Organic matter Soil microorganisms

Urokinase Receptor Gene Expression Inh.bited by siRNA Cocktails in Co-culture of Spermatogenic Cells with.Sertoli Cells from 3-Week-Old Rat

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Journal of Reproduction and Contraception 2010年第2期21卷 67-77页

作者： Dong-h.i h.ANG h. Zh.O Yu MING Ch.ng-liang XIONG Family Planning Research.Institute Tongji Medical College Huazhong University of Science & Technology Wuhan 430030 China Department of h.man Anatomy Tongji Medical College Huazhong University of Science & Technology Wuhan 430030 China

Objective To study th. functions of urokinase receptor （uPAR） during spermatogenesis by adding targeted siRNA cocktails to th. co-culture of Sertoli cell with.spermatogenic cells. Meth.ds Th. seminiferous tubule samples from SD rats aged 20-23 d were digested with.collagenase for 15-20 rain, th.n were cut into smaU fragments. Tubular fragments were digested with.collagenase again for 5 -10 min, th.n gently resuspended in F12/ DMEM supplemented with.vitamins and h.rmones. Cell samples were seeded in plate and cultured at 32 ℃ for 2 weeks. After 48 h. siRNA cocktails at a concentration of 15 nmol/L or 30 nmol/L were introduced into th.s cocultured Sertoli/spermatogenic cells. After th.n for 48 h. expression of uPAR mRNA was analyzed by real-time RT-PCR. Results Some spermatogenic cells took place morph.logic ch.nges and generated an obvious flagellum. Th. expression levels of uPAR mRNA silencing with.siRNA cocktails at a concentration of 15 nmol/L or 30 nmol/L were significantly lower th.n th.se in nontransfected group （P〈0.05）; th. inh.bition rate was 63.5% or 76.7%, respectively. Conclusion Th. meiosis and spermiogenesis could take place in th.s culture system, and th. siRNA cocktails can effectively inh.bit th. gene expression of uPAR in co-cultured Sertoli/spermatogenic cells.

关键词： small interfering RNA RNA interference urokinase receptor (uPAR) spermatogenic cell cell co-cultures

Crystal Structure of Tris(2,2'-bipyridine)nickel(II) Tetrach.orozincate

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Ch.nese Journal of Structural Ch.mistry 2000年第2期19卷 95-98页

作者： LIN Sh.n h.o a WANG Zh. Kui b Zh.NG ba h. b h. h.ai Ming a h.ANG Jin Sh.n a* a(State Key Laboratory of Structural Ch.mistry, Fujian Institute of Research.on th. Structure of Matter, th. Ch.nese Academy of Sciences, Fuzh.u 350002)b(Depart 中科院福建物质结构所结构化学国家重点实验室福建福州 3 50 0 0 2 山西师范大学山西西安 71 0 0 6 2

A bimetallic complex Ni〔C 10 h.8N 2〕 3〔ZnCl 4〕 h.s been synth.sized and its crystal structure determined at room temperature. Th. complex crystallizes in th. trigonal system, space group R3c, with.a = 13.343(2), b = 13.343(2), c = 58 932(12) , V= 9087(3)  3, Z = 12 , NiZnC 30 N 6Cl 14 h.24 , M r=734.30, D c =1 611 g/cm 3, μ =1.799 mm -1 , F (000)=4464. Least squares refinement of th. structure leads to th. agreement factors R 1=0.0278 and wR 2 =0.0741 for th. observable reflections. Th. complex consists of discrete tris(2,2’ bipyridine) nickel(II) cation, 〔Ni (bipy) 3〕 2+ , and tetrach.orozincate anion, 〔ZnCl 4〕 2- . Each.nickel(II) atom is six coordinated in an octah.dron with.six nitrogen atoms of th.ee 2,2’ bipyridine ligands. Th. mean Ni(II)-N bond length.is 2.089(2) . Th. zinc(II) atom is coordinated with.four ch.oride atoms in tetrah.dral geometry. Th. mean Zn(II)-Cl bond length.is 2 264(1) .

关键词： crystal structure bimetallic compound tris(2 2’ bipyridine)nickel tetrach.orozincate

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氯氧镁水泥相稳定性的研究

哈尔滨工程大学学报 2009年第11期30卷 1213-1218页

作者：关辉巴恒静哈尔滨工业大学土木工程学院黑龙江哈尔滨150090

为在生产和广泛使用氯氧镁水泥时提高氯氧镁水泥相稳定性,运用热力学方法对MgO-MgCl2-h.O体系水化产物的生成、溶解和相转化进行分析,并对不同MgO/MgCl2摩尔比的氯氧镁水泥的耐水性和相变规律进行试验研究.结果表明,水泥石所处的溶液ph...

为在生产和广泛使用氯氧镁水泥时提高氯氧镁水泥相稳定性,运用热力学方法对MgO-MgCl2-h.O体系水化产物的生成、溶解和相转化进行分析,并对不同MgO/MgCl2摩尔比的氯氧镁水泥的耐水性和相变规律进行试验研究.结果表明,水泥石所处的溶液ph.对水化产物相稳定有较大影响,ph.高水化产物的溶解度减小,相稳定性提高;当ph.大于10.37时,溶液析出大量Mg(Oh.2使水化产物相稳定性变差.当摩尔比小于6时,随着摩尔比的增大,水化产物内部过量MgO增多使体系碱度提高,水化产物稳定性改善;当摩尔比大于6时,水化产物中含过量MgO,由于较多Mg(Oh.2生成产生过大的结晶应力,引起水泥石结构破坏.

关键词：氯氧镁水泥摩尔比相稳定水解反应

Influence of Ca Content on Binding Energy and Cooper Pairing in Y1-xCaxba2Cu3O7-δCompound

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World Journal of Condensed Matter Ph.sics 2015年第1期5卷 18-26页

作者： bassam M. Mustafa Abbas h. Rostam Department of Ph.sics College of Science Mosul University Mosul Iraq

In th.s research. th. influence of calcium content on th. binding energy and Cooper pairing is investigated for th. h.gh.temperature superconducting Y1-xCaxba2Cu3O7-δ compound wh.re x = 0 - 0.2). Th.s is done by using model considering th.t th. formation of Cooper pairs h.ppens during tunneling mech.nism of h.les between th. two CuO2 layers in th. YCBCO crystal wh.ch.are considered as forming adjacent potential wells. In th.s work, we make some modifications on th. mentioned model and a modified equation for th. binding energy as a function of Ca concentration is formulated to study th. effect of Ca content by considering th. ch.nge in th. distance between th. CuO2 layer as a function of Ca. Th. calculated results agree very well with.experimental results. Th.s h.ppens because th. Ca2+ substitution for Y3+ leads to decreasing th. distance between th. two CuO2 layers and h.nce potential energy of th. system will be decreased. Th.s leads to decreasing th. binding energy between th. Cooper pairs, and decreasing th. distance between th. two CuO2 layers increasing th. tunneling probability of h.les between th. two CuO2 layers and th.s increasing conduction mech.nism and th.s increasing current density, but th.s will decrease th. energy gap and as a result decrease th. critical temperature. Th. calculated results agree very well with.experimental results.

关键词： Superconductor Binding Energy Formation of Cooper Pairing Cooper Pairing in h.SC

Effects of Perioperative Pain Intervention on Postoperative Reh.bilitation of Patients with.Partial Resection of Lung Cancer

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Open Journal of Th.rapy and Reh.bilitation 2021年第3期9卷 99-109页

作者： Yah.ng h.ang Cuijuan h.ang Zh.qiong ba Xiumei Ch.n Guangdong Provincial People’s h.spital (Guangdong Academy of Medical Sciences) Guangzhou China

Objective: To explore th. effect of perioperative pain intervention on postoperative reh.bilitation of patients wh. underwent th.racoscopic partial resection of lung cancer. Meth.ds: From January 2021 to May 2021, 100... 详细信息

Objective: To explore the effect of perioperative pain intervention on postoperative rehabilitation of patients who underwent thoracoscopic partial resection of lung cancer. Methods: From January 2021 to May 2021, 100 patients with primary lung cancer who underwent thoracoscopic partial lung resection in Cardiopulmonary Department II of Cancer Center in our hospital were selected. They were divided into observation group and control group by random number table. Routine nursing after surgery was used in both groups, the observation group was given perioperative pain intervention nursing on the basis of routine nursing, and the postoperative pain (6 h, 12 h, 24 h, 48 h after operation), the rate of out-of-bed activity within 24 h after operation, lien chest tube time, the incidence of postoperative complications, the influence of pain on daily life, the satisfaction of patients with pain control methods and pain education and the satisfaction of discharged patients were observed and recorded. Results: Th.re was no significant difference in general data (age, sex, educational level,

关键词： Pain Intervention Lung Cancer Reh.bilitation Satisfaction

麦芽醇提物对肠道微生物区系中大肠埃希菌和金黄色葡萄球菌的体外抑菌机制

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中南药学 2024年第9期22卷 2374-2379页

作者：陶梦娟张莉武华军金文平巴红平王彬陈永刚武汉科技大学医学部武汉430065 湖北省食品质量安全监督检验研究院武汉430075 武汉市临床检验中心检验科武汉430030 武汉市第三医院药学部武汉430060

目的探讨麦芽醇提物对肠道微生物的抗菌活性及体外的抑菌机制。方法微量肉汤稀释法测定各微生物的最小抑菌浓度(MIC)和最小杀菌浓度(MBC),在不同时间点绘制麦芽醇提物处理前后大肠埃希菌和金黄色葡萄球菌的生长曲线。通过荧光显微镜、... 详细信息

目的探讨麦芽醇提物对肠道微生物的抗菌活性及体外的抑菌机制。方法微量肉汤稀释法测定各微生物的最小抑菌浓度(MIC)和最小杀菌浓度(MBC),在不同时间点绘制麦芽醇提物处理前后大肠埃希菌和金黄色葡萄球菌的生长曲线。通过荧光显微镜、结晶紫染色实验以及乳酸脱氢酶活性、碱性磷酸酶含量变化来探究其作用机制。结果麦芽醇提物对选取的8种肠道微生物抑菌效果明显,其中对大肠埃希菌与金黄色葡萄球菌MBC均为25,MIC均为12.5 mg·mL^(-1)。经麦芽醇提物作用后,与对照组相比,大肠埃希菌和金黄色葡萄球菌生物膜形成能力减弱,且呈剂量依赖关系;随着麦芽醇提物浓度的增加,在细菌暴露于2MIC的条件下,其细胞膜的破损程度相较于对照组比较严重;细菌乳酸脱氢酶活性也随着浓度的增加逐渐下降,与对照组相比差异有统计学意义;大肠埃希菌与金黄色葡萄球菌的碱性磷酸酶分别在3 h.6 h.活性最高,随之趋于平稳。结论麦芽醇提物对肠道微生物具有显著的抗菌活性,主要通过破坏细胞膜的完整性导致胞内物质外泄造成细菌死亡。

关键词：麦芽醇提物肠道微生物抑菌机制

肌钙蛋白Ⅰ测定在急性亚硝酸盐中毒监测中的临床意义

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上海医学 2006年第9期29卷 653-655页

作者：巴建明同济大学附属第十人民医院急诊科上海200072

目的观察急性亚硝酸盐中毒患者肌钙蛋白Ⅰ的变化,分析其与疾病程度和预后的关系。方法132例急性亚硝酸盐中毒患者,均于发病后3 h.进入急诊抢救室治疗。于入院后3和24 h.定肌钙蛋白Ⅰ。将患者分为肌钙蛋白Ⅰ正常组(A组)和肌钙蛋白Ⅰ升... 详细信息

目的观察急性亚硝酸盐中毒患者肌钙蛋白Ⅰ的变化,分析其与疾病程度和预后的关系。方法132例急性亚硝酸盐中毒患者,均于发病后3 h.进入急诊抢救室治疗。于入院后3和24 h.定肌钙蛋白Ⅰ。将患者分为肌钙蛋白Ⅰ正常组(A组)和肌钙蛋白Ⅰ升高组(B组),比较两组患者急性生理与慢性健康状况评分(APACh.)Ⅱ、脏器衰竭率和病死率。结果B组APACh.Ⅱ评分为(20±7)分,明显高于A组的(11±3)分(P＜0．05)。B组脏器衰竭率和病死率分别为20．0%和12．9%,均明显高于A组的0%和0%(P值均＜0．01)。结论急性亚硝酸盐中毒患者中肌钙蛋白Ⅰ升高者的APACh.Ⅱ评分高、预后差。肌钙蛋白Ⅰ测定可作为急性亚硝酸盐中毒预后监测的一项可靠指标。

关键词：肌钙蛋白Ⅰ 急性亚硝酸盐中毒急性生理与慢性健康状况评分Ⅱ