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Cell death and survival following manual and femtosecond laser-assisted capsulotomy in age-related cataract

International Journal of Ophthalmology(English edition) 2018年第9期11卷 1440-1446页

作者： Andrea Krisztina Siikosd Judit Rapp Diatna Feller gyorgy setalo jr Beata Gaspar Judit E.Pongracz Hajnalka Abraham Zsolt Biro Department of Ophthalmology the University of Pecs Medical School and Clinical CentrePecs 7623Hungary Department of Pharmaceutical Biotechnology Faculty of Pharmacythe University of PecsPecs 7624Hungary Janos Szentagothai Research Centre of the University of Pecs Pecs 7624Hungary Department of Medical Biology and Central Electron Microscopic Laboratory the University of Pecs Medical SchoolPecs 7624Hungary Optimum Laser Centre Budapest 1124Hungary

AIM： To study molecular and morphological changes in lens epithelial cells following femtosecond laser-assisted and manually performed continuous curvilinear capsulotomy（CCC） in order to get information about these methods regarding their potential role in the induction of development of secondary cataract. METHODS： Anterior lens capsules（ALC） were removed from 40 patients with age-related cataract by manual CCC and by femtosecond laser-assisted capsulotomy（FLAC）. Samples removed by manual CCC were assorted in group 1, FLAC samples were classified in group 2. Morphology of lens epithelial cells was examined with light and electron microscopes. Following capsulotomy, expressions of p53, Bcl-2 and cyclin D1 genes were analyzed with reverse transcriptase polymerase chain reaction. Immunohistochemistry was used to detect the pro-apoptotic p53 in the epithelial cells. RESULTS： Light and electron microscopic examination showed that ALC of group 1 contained more degenerating cells following manual CCC than after FLAC. The expression level of p53 was higher after manual than laser-assisted surgery. Immunocytochemistry indicated significantly higher number of cells containing p53 protein in the manual CCC group than following FLAC. Bcl-2 and cyclin D1 gene expression levels were slightly lower following manual CCC than after FLAC, but the difference was not significant. CONCLUSION： Manually removed ALC shows slightly, but not significantly larger damage due to the mechanical stretching and pulling of the capsule than those removed using FLAC.

关键词： capsulotomy capsulorhexis femtosecond laser immunhistology ultrastructure gene expression

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Robotic pendant drop:containerless liquid for μs-resolved,AI-executable XPCS

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Light(Science & Applications) 2023年第9期12卷 1857-1866页

作者： Doga Yamac Ozgulbas Don Jensen jr. Rory Butler Rafael Vescovi Ian T.Foster Michael Irvin Yasukazu Nakaye Miaoqi Chu Eric M.Dufresne Soenke Seifert gyorgy Babnigg Arvind Ramanathan Qingteng Zhang Data Science and Learning Division Argonne National LaboratoryLemontIL 60439USA X-ray Science Division Argonne National LaboratoryLemontIL 60439USA Departement of Computer Science University of Chicago5801 S Ellis AveChicagoIL 60637USA XRD Design and Engineering Department Rigaku Corporation 3-9-12 Matsubara-choAkishima-shiTokyo 196-8666Japan Bioscience Division Argonne National LaboratoryLemontIL 60439USA

The dynamics and structure of mixed phases in a complex fluid can significantly impact its material properties,such as ***-angle X-ray Photon Correlation Spectroscopy(SA-XPCS)can probe the spontaneous spatial fluctuations of the mixed phases under various in situ environments over wide spatiotemporal ranges(10−6–103 s/10−10–10−6 m).Tailored material design,however,requires searching through a massive number of sample compositions and experimental parameters,which is beyond the bandwidth of the current coherent X-ray *** 3.7-μs-resolved XPCS synchronized with the clock frequency at the Advanced Photon Source,we demonstrated the consistency between the Brownian dynamics of~100 nm diameter colloidal silica nanoparticles measured from an enclosed pendant drop and a sealed *** electronic pipette can also be mounted on a robotic arm to access different stock solutions and create complex fluids with highly-repeatable and precisely controlled composition *** closed-loop,AI-executable protocol is applicable to light scattering techniques regardless of the light wavelength and optical coherence,and is a first step towards high-throughput,autonomous material discovery.

关键词： resolved sealed mounted

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Surgical stress and cytoskeletal changes in lens epithelial cells following manual and femtosecond laser-assisted capsulotomy

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International Journal of Ophthalmology(English edition) 2020年第6期13卷 927-934页

作者： ANDrea Krisztina Sükosd Krisztina Szabadfi Edina Szabó-Meleg Beáta Gáspár Pavel Stodulka gyorgy Sétálójr Róbert Gábriel Miklós Nyitrai Zsolt Biró Hajnalka Abrahám Department of Ophthalmology University of Pecs Medical School and Clinical CentrePecs 7623Hungary Department of Experimental Zoology and Neurobiology University of PecsPecs 7624Hungary Department of Biophysics University of Pecs Medical School and Clinical CentrePecs 7624Hungary Janos Szentagothai Research Centre University of PecsPecs 7624Hungary Optimum Laser Centre Budapest 1124Hungary Gemini Eye Clinics Zlin 76001Czech Republic Department of Medical Biology and Central Electron Microscopic Laboratory University of Pecs Medical SchoolPecs 7624Hungary

AIM:To study the effect of mechanical stress on the cytoskeleton in lens epithelial cells following conventional phacoemulsification surgery(CPS)and femtosecond laserassisted cataract surgery(FLACS).METHODS:The cytoskeleton of the epithelial cells of the anterior lens capsules(ALC)removed by CPS and FLACS was examined by *** of the intermediate filament,glial fibrillary acidic protein(GFAP),and glutamine synthetase(GS)immunoreactivity were *** order to map the actin network of cells,fluorescently labeled phalloidin was *** samples were examined using confocal laser scanning ***:GFAP expression was visible in a larger number of the epithelial cells after CPS compared to *** CPS sample’s epithelial cells,GFAP immunoreactivity indicated robust morphological *** the actin filaments,the presence of tubular elements connecting epithelial cells,regular actin pattern and marked cortical network after CPS were *** FLACS,the actin cytoskeleton of the epithelial cells remained densely structured,and the tubular elements were undetectable,however,the above-mentioned regular actin pattern and the marked cortical network were ***:The conventional removal of the ALC induces more robust changes of the cytoskeleton of the lens epithelial cells.

关键词： lens epithelial cell cytoskeleton capsulotomy glial fibrillary acidic protein actin

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Discovery of a non-adenylating acyl-CoA ligase and restoring its adenylation activity

Discovery of a non-adenylating acyl-CoA ligase and restoring...

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中国化学会第十二届全国天然有机化学学术会议

作者： Nan Wang Jeffrey D.Rudolf Liao-Bin Dong Jerzy Osipiuk Catherine Hatzos-Skintges Michael Endres Chin-Yuan Chang gyorgy Babnigg ANDrzej Joachimiak George N.Phillips jr Ben Shen Department of Chemistry The Scripps Research Institute Midwest Center for Structural Genomics and Structural Biology Center Biosciences DivisionArgonne National Laboratory Department of Biosciences Rice University Department of Molecular Medicine The Scripps Research Institute Natural Products Library Initiative at The Scripps Research Institute The Scripps Research Institute

Acyl-coenzyme A（CoA） ligases catalyze the activation of carboxylic acids via a two-step reaction of adenylation followed by ***,we report the discovery of a non-adenylating acyl-CoA ligase PtmA2 and the functional separation of an acyl-CoA ligase *** PtmA1 and PtmA2,two acyl-CoA ligases from the biosynthetic pathway of platensimycin and platencin,are necessary for the two steps of CoA *** inactivation of ptmA1 and ptmA2 resulted in the accumulation of free acid and adenylate intermediates,*** and structural characterization of PtmA2 confirmed its ability to only catalyze *** characterization of PtmA2 revealed it binds both free acid and adenylate substrates and undergoes the established mechanism of domain ***,site-directed mutagenesis restored both the adenylation and complete CoA activation *** study challenges the currently accepted paradigm of adenylating enzymes and inspires future investigations on functionally separated acyl-CoA ligases and their ramifications in biology.

关键词： acyl-CoA synthetase acyl-CoA ligase platensimycin platencin

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