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Glutamine prevents oxidative stress in a model of portal hypertension

World Journal of Gastroenterology 2017年第25期23卷 4529-4537页

作者： Gilmara Pandolfo Zabot gustavo franco carvalhal Norma Possa Marroni Francielli Licks Renata Minuzzo Hartmann Vinícius Duval da Silva Henrique Sarubbi Fillmann Department of Surgery School of MedicinePontifical Catholic University of Rio Grande do Sul Department of Gastroenterology School of MedicineFederal University of Rio Grande do Sul Department of Pathology School of MedicinePontifical Catholic University of Rio Grande do Sul

AIM To evaluate the protective effects of glutamine in a model of portal hypertension(PH) induced by partial portal vein ligation(PPVL).METHODS Male Wistar rats were housed in a controlled environment and were allowed access to food and water ad libitum. Twenty-four male Wistar rats were divided into four experimental groups:(1) control group(SO)-rats underwent exploratory laparotomy;(2) control + glutamine group(SO + G)-rats were subjected to laparotomy and were treated intraperitoneally with glutamine;(3) portal hypertension group(PPVL)-rats were subjected to PPVL; and(4) PPVL + glutamine group(PPVL + G)-rats were treated intraperitoneally with glutamine for seven days. Local injuries were determined by evaluating intestinal segments for oxidative stress using lipid peroxidation and the activities of glutathione peroxidase(GPx), endothelial nitric oxide synthase(e NOS) and inducible nitric oxide synthase(i NOS) after *** Lipid peroxidation of the membrane was increased in the animals subjected to PH(P 0.05). The activity of the antioxidant enzyme GTx was decreased in the gut of animals subjected to PH compared with that in the control group of animals not subjected to PH(P 0.05). At least 10 random, non-overlapping images of each histological slide with 200 × magnification(44 pixel = 1 μm) were captured. The sum means of all áreas, of each group were calculated. The mean areas of e NOS staining for both of the control groups were similar. The PPVL group showed the largest area of staining for e NOS. The PPVL + G group had the second highest amount of staining, but the mean value was much lower than that of the PPVL group(P < 0.01

关键词：门高血压 Endothelial 氮的氧化物 synthase 夫酸安谷胱甘肽 peroxidase 可诱导的氮的氧化物 synthase 类脂化合物 peroxidation

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Glutamine prevents oxidative stress in a model of mesenteric ischemia and reperfusion

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World Journal of Gastroenterology 2014年第32期20卷 11406-11414页

作者： Gilmara Pandolfo Zabot gustavo franco carvalhal Norma Possa Marroni Renata Minuzzo Hartmann Vinícius Duval da Silva Henrique Sarubbi Fillmann Department of Surgery School of Medicine Pontifical Catholic University of Rio Grande do Sul Porto Alegre RS 90610-000 Brazil Department of Gastroenterology School of Medicine Federal University of Rio Grande do Sul Porto Alegre RS 90035-903 Brazil Department of Pathology School of Medicine Pontifical Catholic University of Rio Grande do SulPorto Alegre RS 90610-000 Brazil

AIM: To evaluate preventative effects of glutamine in an animal model of gut ischemia/reperfusion(I/R).METHODS: Male Wistar rats were housed in a controlled environment and allowed access to food and water ad libitum. Twenty male Wistar rats were divided into four experimental groups:(1) control group(control)- rats underwent exploratory laparotomy;(2) control + glutamine group(control-GLU)- rats were subjected to laparotomy and treated intraperitoneally with glutamine 24 and 48 h prior to surgery;(3) I/R group- rats were subjected to occlusion of the superior mesenteric artery for 30 min followed by 15 min of reperfusion; and(4) ischemia/reperfusion + glutamine group(G + I/R)- rats were treated intraperitoneally with glutamine 24 and 48 h before I/R. Local and systemic injuries were determined by evaluating intestinal and lung segments for oxidative stress using lipid peroxidation and the activity of superoxide dismutase(SOD), interleukin-6(IL-6) and nuclear factor kappa beta(NFkB) after mesenteric I/R. RESULTS: Lipid peroxidation of the membrane was increased in the animals subjected to I/R(P < 0.05). However, the group that received glutamine 24 and 48 h before the I/R procedure showed levels of lipid peroxidation similar to the control groups(P < 0.05). The activity of the antioxidant enzyme SOD was decreased in the gut of animals subjected to I/R when compared with the control group of animals not subjected to I/R(P < 0.05). However, the group that received glutamine 24 and 48 h before I/R showed similar SOD activity to both control groups not subjected to I/R(P < 0.05). The mean area of NF-kB staining for each of the control groups was similar. The I/R group showed the largest area of staining for NF-kB. The G + I/R group had the second highest amount of staining, but the mean value was much lower than that of the I/R group(P < 0.05). For IL-6, control and control-GLU groups showed similar areas of staining. The I/R group contained the largest area of IL-6 sta

关键词： Ischemia-reperfusion Glutamine Lipid peroxidation

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Raman probing carbon&aqueous electrolytes interfaces and molecular dynamics simulations towards understanding electrochemical properties under polarization conditions in supercapacitors

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Journal of Energy Chemistry 2021年第9期30卷 279-292页

作者： Rafael Vicentini Leonardo M.Da Silva Débora V.franco Willian G.Nunes Juliane Fiates gustavo Doubek Luís F.M.franco Renato G.Freitas Cristiano Fantini Hudson Zanin Advanced Energy Storage Division Carbon Sci-Tech LabsUniversity of CampinasAv Albert Einstein 400CampinasSP 13083-852Brazil Department of Chemistry Federal University of Jequitinhonha and Mucuri’s ValleyRodovia MGT 367km 5835000Alto da Jacuba39.100-000 DiamantinaMGBrazil Advanced Energy Storage Division Center for Innovation on New EnergiesLaboratory of Advanced BatteriesSchool of Chemical EngineeringUniversity of Campinas(UNICAMP)CampinasSP 13083-852Brazil Department of Chemistry Federal University of Mato GrossoICET-UFMT78060-900 CuiabáMTBrazil Departamento de Física ICExUniversidade Federal de Minas GeraisBelo HorizonteMG 31270-901Brazil

Raman probing of carbon electrode and electrolyte under dynamic conditions is performed here using different aqueous electrolytes to elucidate the fundamental events occurring in electrochemical supercapacitor during charge–discharge *** areal capacitance ranges from 1.54 to 2.31μF cm^(-2)μm and it is determined using different *** findings indicate that the Helmholtz capacitance governs the overall charge-storage process instead of the space charge(quantum)capacitance commonly verified for HOPG electrodes in the range of~3 to 7μF cm^(-2).Molecular dynamics simulations are employed to elucidate the origin of the reversible Raman spectral changes during the charge–discharge processes.A correlation is verified between the reversible Raman shift and the surface excesses of the different ionic species.A theoretical framework is presented to relate the effect of the applied potential on the Raman shift and its correlation with the surface ionic *** is proposed that the Raman shift is governed by the interaction of solvated cations with graphite promoted by polarization *** is the first time that a comparative study on different aqueous electrolyte p H and cation ion size has been performed tracking the Raman spectra change under dynamic polarization conditions and contrasting with comprehensive electrochemistry and dynamic molecular simulations *** study shines lights onto the charge-storage mechanism with evidence of Kohn anomaly reduction in the carbon electrode during the reversible adsorption/desorption and insertion/extraction of ionic species.

关键词： Defect reorganization in graphite Surface excess of charge Operando Raman studies Electrical double-layer capacitors

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Fisher's perceptions about a marine protected area over time

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Aquaculture and Fisheries 2020年第5期5卷 273-281页

作者： Cristina Pita Barbara Horta e Costa gustavo franco Rui Coelho Inês Sousa Emanuel J.Gonçalves Jorge M.S.Gonçalves Karim Erzini Department of Environment and Planning&Centre for Environmental and Marine Studies(CESAM) University of AveiroPortugal Center of Marine Sciences CCMARUniversity of AlgarveCampus de Gambelas8005-139FaroPortugal MARE-Marine and Environmental Sciences Centre ISPAInstituto UniversitárioRua Jardim Do Tabaco 341149-041LisboaPortugal Instituto Português Do Mar a da Atmosfera IP(IPMA)-Polo OlhãoAv.5 de Outubro8700-305OlhãoPortugal

The perceptions of fishers towards the Arrábida Marine Park,a marine protected area(MPA)in the west coast of Portugal,were studied through face-to-face interviews in two different moments of the MPA life ***'perceptions about the MPA and the impact it had on the fishing activity over time were identified just before the full implementation of the zoning and regulations of the management plan and 10 years *** study aimed to investigate fishers'knowledge,acceptance and perceptions about the MPA changed with time,if support for the MPA was linked to the impact of the MPA on the fishing activity,and if fishers'perceptions about the impact of the MPA on the fishing activity match with local landings *** show that although knowledge about the marine park significantly improved over time,fishers'acceptance did not.A decrease on fishers'support was not substantial but *** such as the disagreement with regulations reinforced concerns raised during the implementation of the marine park,particularly in relation to the top-down decision-making,which commonly confers minor participation,recognition and legitimacy to ***,fisheries benefits were still not perceived by local fishers,though they are central for fishers'***,the perceived negative impacts of the park seemed to be more related to social aspects and individual interests than to impacts on *** adequate management,enforcement and participation of local fishers is still possible and are advocated here as to contribute to the expected socioecological outcomes and respective support,leading to the future successful performance of the Arrábida Marine *** fishers’perceptions towards an MPA over time is central and should be included on periodical socioecological monitoring and inform an effective adaptive management.

关键词： Arrabida Marine Park(AMP) Local fisheries Perceptions Attitudes Fishers'support Decision-making Marine protected area Changes over time

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Prevalence of metabolic syndrome, obesity and diabetes type 2 in cryptogenic cirrhosis

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World Journal of Gastroenterology 2008年第30期14卷 4771-4775页

作者： Felix I Tellez-Avila Francisco Sanchez-Avila Mauricio García-Saenz-de-Sicilia Norberto C Chavez-Tapia Ada M franco-Guzman gustavo Lopez-Arce Eduardo Cerda-Contreras Misael Uribe Department of Gastroenterology Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

AIM: To evaluate the prevalence of metabolic syndrome (MS), obesity and type 2 diabetes mellitus (T2DM) in a group of Mexican Mestizo patients with cryptogenic cirrhosis (CC) and to compare this group with patients with cirrhosis secondary to other causes (disease controls). METHODS: Patients with CC, diagnosed between January, 1990 and April, 2005, were included in a retrospective study. Patients with cirrhosis caused by chronic hepatitis C, alcohol abuse or autoimmune hepatitis (AIH) served as disease controls. RESULTS: A total of 134 patients with CC were analyzed. Disease controls consisted of 81 patients with chronic hepatitis C, 33 with alcohol abuse and 20 with AIH. The median age of patients with CC was 57 years (range, 16-87); 83 (61.9%) patients were female; 53 (39.6%) were Child A, 65 (48.5%) Child B, and 16 (11.9%) were Child C cirrhosis. The prevalence of MS (29.1% vs 6%; P < 0.001), obesity (16.4% vs 8.2%; P = 0.04) and T2DM (40% vs 22.4%; P = 0.013) was higher in CC patients than in disease controls. There were no differences in sex, age or liver function tests between the two groups. CONCLUSION: The prevalence of MS, obesityand T2DM were higher in patients with CC than in patients with cirrhosis secondary to others causes. Our findings support the hypothesis that non-alcoholic steatohepatitis (NASH) plays an under-recognized role in CC.

关键词：隐原性肝硬化慢性肝炎代谢综合症肥胖糖尿病

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QT prolongation is associated with increased mortality in end stage liver disease

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World Journal of Cardiology 2017年第4期9卷 347-354页

作者： Sun Moon Kim Bennet George Diego Alcivar-franco Charles L Campbell Richard Charnigo Brian Delisle Jonathan Hundley Yousef Darrat gustavo Morales Samy-Claude Elayi Alison L Bailey Division of Cardiovascular Medicine Gill Heart InstituteUniversity of Ken-tucky Department of Cardiology Summa Health System Division of Cardiovascular Medicine Erlanger Health SystemUniversity of Tennessee College of Medicine Chattanooga Departments of Biostatistics and Statistics University of Kentucky Piedmont Transplant Institute

AIM To determine the prevalence of QT prolongation in a large series of end stage liver disease(ESLD) patients and its association to clinical variables and *** The QT interval was measured and corrected for heart rate for each patient,with a prolonged QT cutoff defined as QT > 450 ms for males and QT > 470 ms for *** clinical variables were evaluated including sex,age,serum sodium,international normalized ratio,creatinine,total bilirubin,beta-blocker use,Model for EndStage Liver Disease(MELD),MELD-Na,and etiology of liver disease. RESULTS Among 406 ESLD patients analyzed,207(51.0%) had QT prolongation. The only clinical variable associated with QT prolongation was male gender(OR = 3.04,95%CI:2.01-4.60,P < 0.001). During the study period,187patients(46.1%) died. QT prolongation was a significant independent predictor of mortality(OR = 1.69,95%CI:1.03-2.77,P = 0.039). In addition,mortality was also associated with viral etiology of ESLD,elevated MELD score and its components(P < 0.05 for all). No significant reversibility in the QT interval was seen after liver transplantation. CONCLUSION QT prolongation was commonly encountered in an ESLD population,especially in males,and served as a strong independent marker for increased mortality in ESLD patients.

关键词：肝硬化 Electrophysiology 心律不齐 QT 延伸死亡肝移植

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Determinants of percutaneous coronary intervention success in repeat chronic total occlusion procedures following an initial failed attempt

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World Journal of Cardiology 2017年第4期9卷 355-362页

作者： Cecilia Cuevas Nicola Ryan Alicia Quirós Juan gustavo Del Angel Nieves Gonzalo Pablo Salinas Pilar Jiménez-Quevedo Luis Nombela-franco Ivan Nunez-Gil Antonio Fernandez-Ortiz Carlos Macaya Javier Escaned Cardiovascular Institute Hospital Clinico San Carlos28040 MadridSpain

AIM To investigate the rates and determinants of success of repeat percutaneous coronary intervention(PCI) following an initial failed attempt at recanalising the chronic total occlusions(CTO) *** In 445 consecutive first attempt CTO-PCI procedures in our institution,procedural failure occurred in 149(33.5%). Sixty-four re-PCI procedures were performed in 58 patients(39%) all had a single CTO. Procedural and outcome data in the re-PCI population was entered into the institutional database. A retrospective analysis of clinical,angiographic and procedural data was performed. RESULTS Procedural success was achieved in 41(64%) procedures. Univariate analysis of clinical and angiographic characteristics showed that re-PCI success was associated with intravascular ultrasound(IVUS) guidance(19.5% vs 0%,P = 0.042),while failure was associated with severecalcification(30.4% vs 9.7%,P = 0.047) and a JCTO score > 3(56.5% vs 17.1% P = 0.003). Following multiple regression analysis the degree of lesion complexity(J-CTO score > 3),IVUS use,involvement of an experienced CTO operator and LAD CTO location were significant predictors of successful re-PCI. Overall the complication rate was low,with the only MACCE two periprocedural MI's neither of which required intervention. CONCLUSION Re-PCI substantially increases the overall success rate of CTO revascularization. Predictors of re-PCI success included the use of IVUS,the involvement of an experienced CTO operator in the repeat attempt and the location of the CTO.

关键词： Repeat percutaneous coronary intervention Chronic total occlusion

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Efficacy and safety of endoscopic prophylactic treatment with undiluted cyanoacrylate for gastric varices

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World Journal of Gastrointestinal Endoscopy 2014年第6期6卷 254-259页

作者： Matheus Cavalcante franco gustavo Flores Gomes Frank Shigeo Nakao gustavo Andrade de Paulo Angelo Paulo Ferrari Jr Ermelindo Della Libera Jr Digestive Endoscopy Unit Division of GastroenterologyFederal University of Sao Paulo Endoscopy Division Fleury Medicina e Saúde Endoscopy Division Hospital Israelita Albert Einstein

AIM:To evaluate the efficacy and safety of undiluted N-butyl-2 cyanoacrylate plus methacryloxysulfolane(NBCM)as a prophylactic treatment for gastric varices(GV)***:This prospective study was conducted at a single tertiary-care teaching hospital between October 2009 and March *** with portal hypertension(PH)and GV,with no active gastrointestinal bleeding,were enrolled in primary prophylactic treatment with NBCM injection without lipiodol *** diagnosis of GV was based on endoscopy and confirmed with endosonography(EUS); the same procedure was used after treatment to confirm eradication of *** puncturing the GV with a regular injection needle,1 mL of undiluted NBCM was injected intranasally into *** injection was repeated as necessary to achieve eradication or until a maximum total volume of 3 mL of NBCM had been *** were followed clinically and evaluated with endoscopy at 3,6 and 12 *** follow-ups were performed *** main outcome measures were efficacy(GV eradication),safety(adverse events related to cyanoacrylate injection),recurrence,bleeding from GV and mortality related to GV ***:A total of 20 patients(15 male)with PH and GV were enrolled in the study and treated with undiluted NBCM *** 2(10%)patients had no esophageal varices(EV); 18(90%)patients were treated with endoscopic band ligation to eradicate EV before inclusion in the *** patients were followed clinically and endoscopically for a median of 31 mo(range:6-40 mo).Eradication of GV was observed in all patients(13 patients were treated with 1 session and 7 patients with 2 sessions),with a maximum injected volume of 2 mL *** patient had GV recurrence,confirmed by EUS,at 6-mo follow-up,and another had late recurrence with GV bleeding after 35 mo of follow-up; overall,GV recurrence was observed in 2 patients(10%),after 6 and 35 mo of follow-up,and GV bleeding rate was 5%(1 patient).Mild epigastric pain was r

关键词： Gastric varices Primary prevention Endoscopy Gastrointestinal Cyanoacrylates Gastrointestinal hemorrhage

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Cellulose Acetate Film and Layered Double Hydroxide as Determination Method of Available Phosphorus in the Soil

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Journal of Agricultural Science and Technology(B) 2018年第7期8卷 420-432页

作者： gustavo franco de Castro Jader Alves Ferreira Allan Robledo Fialho e Moraes Reinaldo Bertola Cantarutti Frederico Garcia Pinto Jairo Tronto Roberto Ferreira Novais Department of Soil University of VicosaAv.Peter Henry RolfsBulding Silvyo Starling BrandaoCEP 36570-000Vicosa(MG)Brazil Department of Chemistry University of VicosaRio Paranaiba CampusHighway MG-230KM 07CEP 38810-000Rio Paranaiba(MG)Brazil

The determination of available phosphorus(P)in soil is essential to the evaluation of the current fertility status,and therefore,for recommending the required phosphate fertilizers for the cultivation of *** mostly used extractants around the country are Mehlich-1(M-1)and anionic resins(AR)and mixed resins(MR).However,there are still controversies in relation to the method that should be used,due to of particularities of each ***,the main objectives of this work was to assess the applicability of films of binary combination between cellulose acetate(CA)biopolymer and calcinated layered double hydroxide(LDH-c),named CAF-LDH-c,as a new extractor of available P in *** this,two experiments were set *** the first one,sub-samples of an Oxisol layer 0-20 cm were used and submitted to increasing doses of total P(0,250,750 and 2,250 mg/dm3)in the form of monoammonium phosphate(MAP)at different incubation time(5,30,60 and 90 d).In the second experiment,samples of 20 soils from different origins and contents of available P were used with distinct chemical and physical *** analysis of available P by M-1,AR,MR,Mehlich-3(M-3)and CAF-LDH-c extractants was done in both *** efficiency of the extractants was evaluated by Pearson’s linear *** correlation between values of P extracted from soil in the first experiment was highly significant among all extraction methods used,including for *** the second experiment,CAF-LDH-c showed significant correlation(p<0.01)with M-1,M-3,AR and ***,the significant correlations of the extractant being tested(CAF-LDH-c)with those usually used indicates the possibility of this new method of routine laboratories for assessing the P availability of for the cultivation of crops.

关键词： Available P Mehlich-1 Mehlich-3 anionic resin mixed resin

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Organic-Inorganic Hybrid Materials:Layered Double Hydroxides and Cellulose Acetate Films as Phosphate Recovery

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Journal of Agricultural Science and Technology(B) 2018年第6期8卷 360-374页

作者： gustavo franco de Castro Jader Alves Ferreira Denise Eulálio Allan Robledo Fialho e Moraes Vera Regina Leopoldo Constantino Frederico Garcia Pinto Roberto Ferreira Novais Jairo Tronto Department of Soil University of VicosaAv.Peter Henry RolfsBulding Silvyo Starling BrandaoCEP 36570-000Vicosa(MG)Brazil Department of Chemistry University of VicosaRio Paranaiba CampusHighway MG-230KM 07CEP 38810-000Rio Paranaiba(MG)Brazil Department of Fundamental Chemistry University of Sao PauloAv.Prof.Lineu Prestes748CEP 05508-000Sao Paulo(SP)Brazil

With demand increasing for phosphate recovery,a considerable share of current research is dedicated to elaborate multifunctional *** this way,the objective of this work was to produce hybrid films from the combination of cellulose acetate(CA)biopolymer and magnesium and aluminium layered double hydroxides(LDHs)and investigate their performance for phosphate *** this work,the following materials were prepared and characterized:LDH,calcinated LDH(LDH-c),CA film(CAF),CA film with LDH(CAF-LDH)and CA film with LDH-c(CAF-LDH-c).The produced materials were characterized by X-ray diffraction,thermogravimetric analysis coupled with differential scanning calorimetry and mass spectrometry,attenuated total reflectance Fourier transform infrared spectroscopy and scanning electronic *** thickness,H2O absorption(AH2O),stability in H2O and phosphate adsorption of the produced films were *** adsorption capacity of films was compared to LDHs in powder *** CAF-LDH and CAF-LDH-c increased film thickness,where CAF-LDH-c was thicker than ***-LDH-c had higher AH2O than the other films,because of its increased thickness and mainly because of the H2O sorption process of these materials.H2O stability of 98.97%for CAF-LDH and 96.81%for CAF-LDH-c suggest the produced films can maintain their structural properties even after a long contact period with aqueous *** the CAF-LDH-c,the maximum adsorption capacity of phosphate,according to the Langmuir-Freundlich model,was 6.98 mg/*** adsorption value suggests that this film can be used as an efficient phosphorus(P)adsorbent from wastewater or a eutrophicated source.

关键词： Cellulose acetate films layered double hydroxides phosphorous adsorption phosphorous recovery

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