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威廉环毛蚓中8种核苷提取工艺优化及含量测定

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中成药 2024年第8期46卷 2526-2530页

作者：宇泉霖吴雪纯邱旖宋嘉佳蒋巧萍孙常胜吴婧楠蔡程科王洪飞北京中医药大学中药学院北京102400 北京量子高科制药有限公司北京102200

目的优化威廉环毛蚓中尿嘧啶、次黄嘌呤、黄嘌呤、尿苷、胸腺嘧啶、肌苷、鸟苷、2′-脱氧鸟苷提取工艺,并测定其含量。方法以料液比、超声时间、超声温度为影响因素,次黄嘌呤、总核苷含量为评价指标,正交试验优化提取工艺。HPLC法测定... 详细信息

目的优化威廉环毛蚓中尿嘧啶、次黄嘌呤、黄嘌呤、尿苷、胸腺嘧啶、肌苷、鸟苷、2′-脱氧鸟苷提取工艺,并测定其含量。方法以料液比、超声时间、超声温度为影响因素,次黄嘌呤、总核苷含量为评价指标,正交试验优化提取工艺。HPLC法测定各核苷含量,分析采用Agilent C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相甲醇-水,梯度洗脱;体积流量1 mL/min;柱温30℃;检测波长260 nm。结果最佳条件为料液比1∶250,超声时间30 min,超声温度60℃。8种核苷在各自范围内线性关系良好(R^(2)>0.9990),平均加样回收率99.11%~103.27%,RSD 0.85%~2.89%。结论该方法稳定可靠,可用于威廉环毛蚓中核苷的提取与含量测定。

关键词：威廉环毛蚓核苷提取工艺含量测定正交试验 HPLC

Electroacupuncture alleviates ciliary muscle cell apoptosis in lens-induced myopic guinea pigs through inhibiting the mitochondrial signaling pathway

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International Journal of Ophthalmology(English edition) 2023年第12期16卷 1942-1951页

作者： Zhao-Hui Yang Jia-Wen Hao Jin-Peng Liu Bo Bao Tu-Ling Li Qiu-Xin wu Ming-Guang He Hong-sheng Bi Da-Dong guo Shandong University of Traditional Chinese Medicine Jinan 250014Shandong ProvinceChina Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine Shandong Provincial Clinical Medical Research Center of Optometry and Adolescent Low Vision Prevention and ControlJinan 250002Shandong ProvinceChina State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou 510060Guangdong ProvinceChina Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases Shandong Academy of Eye Disease Prevention and TherapyMedical College of Optometry and OphthalmologyShandong University of Traditional Chinese MedicineJinan 250002Shandong ProvinceChina

AIM:To investigate the effect of electroacupuncture(EA)on the mitochondria-dependent apoptotic signaling pathway in the ciliary muscle of guinea pigs with negative lensinduced myopia(LIM).METHODS:Guinea pigs were randomly divided into normal control(NC)group,LIM group,LIM+SHAM acupoint(LIM+SHAM)group,and LIM+EA *** in the NC group received no intervention,while those in other three groups were covered with-6.0 diopter(D)lenses on right ***,animals in the LIM+EA group received EA at Hegu(LI4)combined with Taiyang(EX-HN5)acupoints,while those in the LIM+SHAM group were treated at sham *** treatments for 1,2,and 4wk,morphological changes in ciliary muscles were observed with hematoxylin and eosin(H&E)staining and nick end labeling(TUNEL),and the expression of the mitochondrial apoptotic signaling pathway-related molecules in ciliary muscles was measured by real-time quantitative polymerase chain reaction(qPCR)and Western ***,the adenosine triphosphate(ATP)contents were also determined in ciliary ***:Axial length increased significantly in the LIM and LIM+SHAM groups and decreased in the LIM+EA *** ciliary muscle fibers were broken and destroyed in both LIM and LIM+SHAM groups,whereas those in the LIM+EA group improved *** assay showed the number of apoptotic cells increased in the LIM and LIM+SHAM groups,whereas reduced in the LIM+EA *** contents showed a significant decrease in the LIM and LIM+SHAM groups,whereas increased after EA *** with the NC group,the dynamin-related protein 1(DRP1),Caspase3,and apoptotic protease activator 1(APAF1)levels were significantly increased in the LIM group and decreased in the LIM+EA ***:The results provide evidence of EA inhibiting the development of myopia by regulating the mitochondrial apoptotic signaling pathway.

关键词： myopia electroacupuncture ciliary muscle apoptosis mitochondrial signaling pathway guinea pigs

Fuzzy Evaluation of Coal Seam Geological Condition of Fully-Mechanized Face in Ten-Million-Ton Mine

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Journal of China University of Mining and Technology 2005年第3期15卷 208-212页

作者： LI Wei-min DONG guo-wei WANG Hong-sheng FENG Mei-mei School of Mineral and Safety Engineering China University of Mining ＆ Technology Xuzhou Jiangsu 221008 China Jisan Mine of Yanzhou Mining Group Jining Shandong 272100 China

Based on coal seam geological condition and mining technological characteristic in Jisan Mine, coal seamgeological condition is quantitative evaluated by using fuzzy evaluation with the view of coal mining and coalfaceproduction. The structure and index system of evaluation factor, the membership functions and weights of evaluationfactor, evaluation model and reliability in the coal seam geological conditions are expounded in detail. Eighty-twocoalfaces which will be exploited is classified. All of these have provided a theoretical foundation for the selection ofcoal mining technology and for sustainable development of the coal mine.

关键词： fuzzy evaluation membership function ten-million-ton mine

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食管癌手术治疗及预后研究进展

食管疾病 2024年第1期6卷 49-55页

作者：郭一君高社干丁晓宁盛漫曦河南科技大学临床医学院河南科技大学第一附属医院洛阳471003

食管癌是我国危害国民健康的重大疾病之一,切除或破坏瘤体是治疗食管癌的基本方法。食管癌手术治疗包括开放式切除术、腔镜微创切除术以及内镜下的切除和非切除疗法等。根据肿瘤的大小、部位、类型和患者的身体状况、疾病分期等选择合... 详细信息

食管癌是我国危害国民健康的重大疾病之一,切除或破坏瘤体是治疗食管癌的基本方法。食管癌手术治疗包括开放式切除术、腔镜微创切除术以及内镜下的切除和非切除疗法等。根据肿瘤的大小、部位、类型和患者的身体状况、疾病分期等选择合适的术式,预防和减少并发症,是延长生存期、提高生存质量、改善预后的基本途径。本文结合国内外进展,总结当前临床上在用的各种食管癌治疗术,以及各种术式的预后及其影响因素等,供临床治疗方案选择和相关研究者参考。

关键词：食管癌手术预后术后并发症

基于中国水土保持区划的江苏省植被盖度监测点布局

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中国水土保持科学 2024年第4期22卷 171-181页

作者：谭露郭红丽刘霞吴芳张雪蒙雯婷陈建鹏南京林业大学林学院南京210037 南京林业大学南方现代林业协同创新中心南京210037 江苏省水文水资源勘测局南京210005

植被盖度是土壤侵蚀监测与评价的重要因子。植被空间分异性是影响植被盖度分布的主要因素。以江苏省为研究区,以全国水土保持区划的三级区为控制单元,采用多源数据空间分析、分层抽样和野外调查相结合的方法,分析植被类型、林种与树种... 详细信息

植被盖度是土壤侵蚀监测与评价的重要因子。植被空间分异性是影响植被盖度分布的主要因素。以江苏省为研究区,以全国水土保持区划的三级区为控制单元,采用多源数据空间分析、分层抽样和野外调查相结合的方法,分析植被类型、林种与树种的空间分异性,提出植被盖度监测点布局方案。结果表明:1)鲁中南低山丘陵土壤保持区(Ⅲ-4-2t)、黄泛平原防沙农田防护区(Ⅲ-5-3fn)及淮北平原岗地农田防护保土区(Ⅲ-5-4nt)地带性植被为栎类落叶林;江淮丘陵岗地农田防护保土区(V-1-2nt)、江淮下游平原农田防护水质维护区(V-1-1ns)、沿江丘陵岗地农田防护人居环境维护区(V-1-5nr)以及太湖丘陵平原水质维护人居环境维护区(V-1-4sr)北部地带性植被从含有常绿灌木的落叶阔叶林演变为常绿落叶阔叶混交林;V-1-4sr南部地带性植被为常绿阔叶林;2)研究区用材林占53.45%,其次为防护林和经济林,分别占26.06%和13.10%,各水土保持三级区林种、树种的比例结构差异较大,自北向南用材林和防护林由杨树、侧柏等转变为马尾松、杉木、香樟以及以麻栎、栓皮栎、枫香、青冈等为优势树种的阔叶混交林等,经济林由苹果、梨、板栗、银杏等转变为茶、桃、柑橘等;3)基于水土保持三级区林种与树种的空间分异性,以17个县(区)为抽样区获取96个抽样监测点,通过野外调查确定49个植被盖度监测点,涵盖各区划的主要林种与树种。研究结果可为江苏省开展植被盖度监测提供技术支撑,也可为其他区域布设植被盖度监测点提供借鉴。

关键词：植被盖度空间分布植被类型水土保持区划水土流失监测

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聚磷酸盐在灰漠土和砖红壤中吸附-解吸附特性研究

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中国土壤与肥料 2024年第7期 101-107页

作者：张静雯迟海龙吴胜彪马林江席亮危常州张新疆石河子大学农学院新疆石河子832003

通过研究不同聚合度聚磷酸盐在两种不同类型土壤中的磷吸附-解吸特性,为聚磷酸磷肥肥效差异的解析和农业磷肥的应用提供理论依据。以北方灰漠土和南方砖红壤为供试土壤,以常规水溶磷肥(DAP)为对照,探究不同聚合度聚磷酸盐(APP1、APP2、A... 详细信息

通过研究不同聚合度聚磷酸盐在两种不同类型土壤中的磷吸附-解吸特性,为聚磷酸磷肥肥效差异的解析和农业磷肥的应用提供理论依据。以北方灰漠土和南方砖红壤为供试土壤,以常规水溶磷肥(DAP)为对照,探究不同聚合度聚磷酸盐(APP1、APP2、APP3、KTPP)在两种土壤中磷的吸附和解吸附特性,并进一步探究不同类型土壤理化性质与土壤磷吸附-解吸附量之间的相互关系。结果表明:在酸性土壤中,各处理土壤磷吸附能力表现为APP2>APP3>KTPP>APP1>DAP,土壤中磷解吸附能力为APP1>KTPP>DAP>APP3>APP2;在灰漠土中,各处理土壤磷吸附能力表现为APP2>APP1>APP3>DAP>KTPP,土壤中磷解吸附能力为KTPP>APP1>DAP>APP3>APP2。土壤各理化性质参数中土壤黏粒、铁铝氧化物含量与土壤磷最大吸附量(Q_(m))呈正相关关系。土壤pH、有机质和碳酸钙含量与Q_(m)呈负相关关系。综上所述,较高聚合度的聚磷酸盐(KTPP)在灰漠土中磷吸附能力弱,土壤磷有效性高,而较低聚合度的聚磷酸盐(APP1)在酸性土壤中的磷吸附能力弱,能保持较好的土壤磷平衡和供磷能力。土壤pH、有机质和碳酸钙含量越高,土壤黏粒和铁铝氧化物含量越低,则土壤对磷的吸附能力越低,磷的解吸越高,土壤磷有效性越高。

关键词：聚磷酸铵吸附解吸附

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Efficient sampling methods for characterizing POIs on maps based on road networks

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Frontiers of Computer Science 2018年第3期12卷 582-592页

作者： Ziting ZHOU Pengpeng ZHAO Victor S. sheng Jiajie XU Zhixu LI Jian wu Zhiming CUI School of Computer Science and Technology Soochow University Suzhou 215006 China Department of Computer Science University of Central Arkansas Conway AR 72035 USA Jiangsu Engineering Center of Network Monitoring Nanjing University of Information Science & Technology Nanjing 210044 China

With the rapid development of location-based services, a particularly important aspect of start-up marketing research is to explore and characterize points of interest （PoIs） such as restaurants and hotels on maps. However, due to the lack of direct access to PoI databases, it is necessary to rely on existing APIs to query Pols within a region and calculate PoI statistics. Unfortunately, public APIs generally im- pose a limit on the maximum number of queries. Therefore, we propose effective and efficient sampling methods based on road networks to sample PoIs on maps and provide unbiased estimators for calculating PoI statistics. In general, the more intense the roads, the denser the distribution of PoIs is within a region. Experimental results show that compared with state-of-the-art methods, our sampling methods improve the efficiency of aggregate statistical estimations.

关键词： sampling aggregate statistical estimation roadnetworks

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拉锥光纤在传感中的弯曲损耗

华东理工大学学报（自然科学版） 2024年第4期50卷 619-625页

作者：郭嘉豪武明明叶安李晓林钮月萍龚尚庆华东理工大学物理学院上海200237

拉锥光纤在光学传感,尤其是压力传感器方面应用广泛,这是利用了光纤受到外力弯曲后的功率损失。采用有限元方法模拟了绝热直线型拉锥光纤各个位置,特别是锥形过渡区域的弯曲损耗。数值模拟结果表明,相较于其他区域,光纤的芯模截止区弯... 详细信息

拉锥光纤在光学传感,尤其是压力传感器方面应用广泛,这是利用了光纤受到外力弯曲后的功率损失。采用有限元方法模拟了绝热直线型拉锥光纤各个位置,特别是锥形过渡区域的弯曲损耗。数值模拟结果表明,相较于其他区域,光纤的芯模截止区弯曲损耗更大,压力灵敏度更高,这是因为该位置的临界锥角取值最小,绝热条件在弯曲下更容易被破坏。该结果将为拉锥光纤传感器设计中传感点的选取提供有价值的参考。

关键词：拉锥光纤光学传感弯曲损耗受力形变

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Intrahepatic Glissonian approach and outflow vascular occlusion during partial hepatectomy

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Hepatobiliary & Pancreatic Diseases International 2014年第1期13卷 101-104页

作者： Feng Xia guo Li Wan-Yee Lau Kuan-sheng Ma Ping Bie Institute of Hepatobiliary Surgery Southwest HospitalSouthwest Cancer Center Third Military Medical University Faculty of Medicine the Chinese University of Hong Kong

Under ultrasound guidance, a blunt suture needle was inserted around the Glissonian pedicle and then sutured. This technique significantly reduced the blood loss and facilitated the procedure of partial hepatectomy. We applied this technique in 182 patients who needed partial hepatectomy. We concluded that this method is simple and easy to occlude the vascular inflow and outflow, and allows an accurate delineation of the anatomic zone and therefore, simplifies the procedure of partial hepatectomy.

关键词： hepatectomy the Glissonian approach blunt needle surgical technique