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Evaluation of water management on arsenic methylation and volatilization in arsenic-contaminated soils strengthened by bioaugmentation and biostimulation

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Journal of Environmental Sciences 2024年第3期137卷 515-526页

作者： Peng Chen Yi Liu guo-xin sun State Key Laboratory of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China Key Laboratory of Aquatic Botany and Watershed Ecology Wuhan Botanical GardenChinese Academy of SciencesWuhan 430074China University of Chinese Academy of Sciences Beijing 100049China

Arsenic(As) fate in paddy fields has been one of the most significant current issues due to the strong As accumulation potential of rice plants under fooded conditions. However,no attempt was done to explore As methylation and volatilization under non-fooded conditions. Herein, we investigated the effects of water management on As methylation and volatilization in three arsenic-contaminated soils enhanced by biostimulation with strawderived organic matter and bioaugmentation with genetic engineered Pseudomonas putida KT2440(GE P. putida). Under fooded conditions, the application of biochar(BC), rice straw(RS)and their combination(BC+RS) increased total As in porewater. However, these effects were greatly attenuated under non-fooded conditions. Compared with RS amendment alone, the combination of GE P. putida and RS further promoted the As methylation and volatilization,and the promotion percentage under non-fooded conditions were significantly higher than that under fooded conditions. The combined GE P. putida and RS showed the highest efficiency in As methylation(88 μg/L) and volatilization(415.4 μg/(kg·year)) in the non-fooded soil with moderate As contamination. Finally, stepwise multiple linear regression analysis presented that methylated As, DOC and p H in porewater were the most important factors contributing to As volatilization. Overall, our findings suggest that combination of bioaugmentation with GE P. putida and biostimulation with RS/BC+RS is a potential strategy for bioremediation of arsenic-contaminated soils by enhancing As methylation and volatilization under non-fooded conditions.

关键词： Arsenic methylation Arsenic volatilization Water management Straw incorporation Bioaugmentation

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Extraction study of rare earth elements with N,N'-dibutyl–N,N'-di(1-methylheptyl)-diglycolamide from hydrochloric acid

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Nuclear Science and Techniques 2016年第3期27卷 186-193页

作者： guo-Jing sun Jin-Hong Yang Hong-Xiao Yang guo-xin sun Yu Cui School of Chemistry and Chemical Engineering University of Jinan

A novel,unsymmetrical diglycolamide,N,N'-dibutyl-N,N'-di(1-methylheptyl)-diglycolamide(DBD1MHDGA),was *** extraction of rare earth elements(REEs) from a hydrochloric acid medium with DBD1 MHDGA was *** results of the extraction experiments indicated that the distribution ratios of RE(Ⅲ) ions increase with an increase in HC1 concentration,atomic number,and extractant *** calculated thermodynamic data show that the extraction process is an exothermic *** organic phase loaded rare earth ions were characterized by infrared *** composition of the extracted complex was determined.

关键词：稀土元素盐酸介质萃取剂庚基红外光谱表征盐酸浓度稀土离子分配比例

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Solvent extraction of U(Ⅵ) by N,N-dimethyl-N',N'-dioctylsuccinylamide and N,N-dimethyl-N',N'-didecylsuccinylamide in cyclohexane

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Nuclear Science and Techniques 2016年第3期27卷 68-73页

作者： Yu Cui Xiao-Feng Yang Cheng Yang Ye-xin Li guo-Zhu Chen guo-xin sun School of Chemistry and Chemical Engineering University of Jinan

The extraction of uranyl nitrate by novel extractants of N,N-dimethyl-N',N'-dioctylsuccinylamide(DMDOSA)and N,N-dimethyl-N',N'-didecylsuccinylamide(DMDDSA) from aqueous nitric/nitrate solutions was *** was found both the concentration of HNO_3 and extractants had an effect on the U(Ⅵ) extraction *** extraction mechanism was established,and stoichiometry of the main extracted species was confirmed to be UO_2(NO_3)_2·2DMDOSA and UO_2(NO_3)_2·2DMDDSA,*** of the extraction reactions are *** spectral study of the U(Ⅵ) extracted species was also made.

关键词：溶剂萃取二甲基环己烷 ftir光谱硝酸铀酰化学计量比 O3浓度萃取反应

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Responses of soil ammonia oxidizers to a short-term severe mercury stress

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Journal of Environmental Sciences 2015年第12期27卷 8-13页

作者： Zhi-Feng Zhou Yu-Rong Liu guo-xin sun Yuan-Ming Zheng College of Resources and Environment Southwest University State Key Laboratory of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of Sciences

The responses of soil ammonia-oxidizing archaea（AOA） and ammonia-oxidizing bacteria（AOB） to mercury（Hg） stress were investigated through a short-term incubation *** with four different concentrations of Hg（CK,Hg25,Hg50,and Hg100,denoting 0,25,50,and 100 mg Hg/kg dry soil,respectively）,samples were harvested after 3,7,and 28 day *** showed that the soil potential nitrification rate（PNR） was significantly inhibited by Hg stress during the ***,lower abundances of AOA（the highest in CK： 9.20 × 10~7 copies/g dry soil; the lowest in Hg50： 2.68 × 10~7 copies/g dry soil） and AOB（the highest in CK： 2.68 × 10~7 copies/g dry soil; the lowest in Hg50：7.49 × 10~6 copies/g dry soil） were observed only at day 28 of incubation（P 〈 0.05）.Moreover,only the community structure of soil AOB obviously shifted under Hg stress as seen through DGGE profiles,which revealed that 2-3 distinct AOB bands emerged in the Hg treatments at day *** summary,soil PNR might be a very useful parameter to assess acute Hg stress on soil ecosystems,and the community structure of soil AOB might be a realistic biological indicator for the assessment of heavy metal stress on soil ecosystems in the future.

关键词： Mercury (Hg) Potential nitrification rate (PNR) Amonia oxidizers Abundance Community structure

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Extraction of lanthanide ions with N,N,N′,N′-tetrabutyl-3-oxadiglycolamide from nitric acid media

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Nuclear Science and Techniques 2017年第6期28卷 190-195页

作者： Xiu-Jing Peng Yu Cui Ji-Fei Ma Yulan Li guo-xin sun School of Chemistry and Chemical Engineering University of Jinan Shanghai Institute of Applied Physics Chinese Academy of Sciences

Extractability and extraction mechanism of lanthanide ions were investigated by using a new extractant,N,Nn,N′,N′-tetrabutyl-3-oxa-diglycolamide(TBDGA),in toluene from nitric acid *** effects of HNO_3 and TBDGA concentrations,and temperature,on extraction of lanthanide ions were *** of the main extracted species were HNO_3·TBDGA and M(NO_3)_3-3TBDGA(M = Er,Dy,Tb,Gd,La,Ce,Nd,Sm and Eu).The extracted species for metal ions were established to be ionic *** this complex,nitrate anion was not coordinated to the central *** extraction pattern increased gradually across the lanthanide ions series,showing enhanced affinity of TBDGA toward heavy lanthanide *** parameters were investigated for the exothermic extraction reaction.

关键词： Lanthanide ions Extraction N,N,N,N-tetrabutyl-3-oxa-diglycolamide Nitric acid Toluene

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Rapid evaluation of arsenic contamination in paddy soils using field portable X-ray fluorescence spectrometry

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Journal of Environmental Sciences 2018年第2期30卷 345-351页

作者： Jian-Hong Liang Pan-Pan Liu Zheng Chen guo-xin sun Hong Li Key Laboratory of Karst Ecosystem and Treatment of Rocky Desertification Institute of Karst Geology Chinese Academy of Geological Sciences Guilin 541004 China State Key Joint Laboratory of Environment Simulation and Pollution Control School of Environment Tsinghua University Beijing 100084 China State Key Laboratory of Urban and Regional Ecology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China Department of Environmental Science Xi' an Jiaotong-Liverpool University Suzhou 215123 China College of Urban Construction and Environmental Engineering Chongqing University Chongqing 400045 China

Arsenic（As） in paddy fields is deteriorating food security and human health through rice ingestion. Rice is the dominant food source of arsenic exposure to half of the world’s population. Therefore, an in situ effective method for As risk evaluation in paddy soil is strongly needed to avoid As exposure through rice ingestion. Herein, we developed a rapid analytical methodology for determination of As in plant tissues using field portable X-ray fluorescence spectrometry（FP-XRF）. This method was applied to rice roots in order to evaluate the As contamination in paddy soils. The results showed that rice roots with iron plaques were superior to rhizosphere soils for generating FP-XRF signals, especially for field sites with As concentrations lower than the soil detection limit of FP-XRF（30.0 mg/kg）.Moreover, the strong linear relationships of As concentrations between the rice roots and corresponding leaves and grains proved that the rice root, rather than the soil, is a better predictor of As concentrations in rice grains. The research provides an efficient As monitoring method for As contaminated paddy fields by using wetland plant roots with iron plaques and XRF-based analytical techniques.

关键词： XRF Arsenic Risk evaluation Rice roots Rhizosphere soils Iron plaque

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Separation of hafnium from zirconium in hydrochloric acid solution with di(2-ethylhexyl)phosphoric acid by solvent extraction

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Nuclear Science and Techniques 2019年第2期30卷 53-59页

作者： Tao Yang guo-xin sun Yuan Qian Ji-Fei Ma Yu-Lan Li School of Chemistry and Chemical Engineering University of Jinan Shanghai Institute of Applied Physics Chinese Academy of Sciences

The preparation of nuclear-grade zirconium and hafnium is very important for nuclear power. The separation of hafnium from zirconium in a hydrochloric acid solution by solvent extraction was investigated with di(2-ethylhexyl)phosphoric acid(D2 EHPA). The effects of hydrochloric acid concentration, extractant concentration,diluents, and temperature on the distribution coefficient of hafnium and zirconium were studied. The species extracted were ZrOA_2·2 HA and HfOA_2-2 HA. In this process, the separation factors varied with different diluents and followed the order octane > hexane > toluene > chloroform.A high separation factor value of 4.16 was obtained under the conditions of a solution containing 0.05 mol/L HCl and0.01 mol/L D2 EHPA for the separation of hafnium from zirconium. The extraction reaction was endothermic.

关键词： Zirconium Hafnium D2EHPA Solvent extraction Separation

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ZrO_2超细粉的烧结及相关过程研究

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无机材料学报 1993年第2期8卷 163-169页

作者：郭新孙尧卿崔昆华中理工大学

本文研究了 ZrO_2超细粉的烧结过程,并提出了相应的烧结模型.研究的结果表明,粉末团聚体内的粉末晶体之间发生优先烧结,根据热力学分析,这种优先烧结是通过同类型表面相互结合而实现的,它对于正常的烧结过程没有干扰作用,而粉末团聚体... 详细信息

本文研究了 ZrO_2超细粉的烧结过程,并提出了相应的烧结模型.研究的结果表明,粉末团聚体内的粉末晶体之间发生优先烧结,根据热力学分析,这种优先烧结是通过同类型表面相互结合而实现的,它对于正常的烧结过程没有干扰作用,而粉末团聚体之间的烧结对试样的致密化具有重大影响.同时,本文也讨论了试样表面气孔优先排除及试样表面平坦化这两个相关过程,根据热力学分析,认为这两个过程的作用机理可能是表面扩散及蒸发/凝结.

关键词：超细粉烧结氧化锆

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ZrO_2基固体电解质的时效特性研究

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无机材料学报 1993年第3期8卷 295-302页

作者：郭新孙尧卿崔昆华中理工大学

本文用交流阻抗法测量了 ZrO_2材料在940℃、时效222h 的过程中电阻率的变化,并用 XRD、EPMA、EDAX 及 TEM 分析了产生这种变化的原因.研究的结果表明,在时效的初期,ZrO_2晶粒中析出一种微细相,使得晶粒电阻率变大;在时效的后期,ZrO_2... 详细信息

本文用交流阻抗法测量了 ZrO_2材料在940℃、时效222h 的过程中电阻率的变化,并用 XRD、EPMA、EDAX 及 TEM 分析了产生这种变化的原因.研究的结果表明,在时效的初期,ZrO_2晶粒中析出一种微细相,使得晶粒电阻率变大;在时效的后期,ZrO_2晶界相发生相变,又使其晶界电阻率增大.1.5AYZ 试样在500℃时测量的电阻率值的时效动力学方程可表示为:ρ(t)=1962+0.81t^(1.1)(Ω·cm)

关键词：时效电阻率氧化锆固体电解质

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Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying sun Haoyan Li Dongqing sun Shaliu Fu Lei Gu xin Shao Qinqin Wang xin Dong Bin Duan Feiyang xing Jun Wu Minmin Xiao Fangqing Zhao Jing-Dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

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