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Effects of recombinant human growth hormone on enterocutaneous fistula patients

World Journal of Gastroenterology 2008年第44期14卷 6858-6862页

作者： guo-sheng gu jian-an ren ning li jie-shou li The Research Institute of General Surgery Clinical School (Jinling Hospital) School of Medicine Nanjing University Nanjing 210002 Jiangsu Province China

AIM： To explore the effects of recombinant human growth hormone （rhGH） on intestinal mucosal epithelial cell proliferation and nutritional status in patients with enterocutaneous fistula. METHODS： Eight patients with enterocutaneous fistulas received recombinant human growth hormone （10 ug/d） for 7 d. Image analysis and immunohistochemical techniques were used to analyse the expression of proliferating cell nuclear antigen （PCNA） in intestinal mucosal epithelial cells in biopsy samples from the patients who had undergone an endoscopic biopsy through the fistula at day 0, 4 and 7. Body weights, nitrogen excretion, serum levels of total proteins, albumin, prealbumin, transferrin and fibronectin were measured at day 0, 4 and 7. RESULTS： Significant improvements occurred in the expression of PCNA in the intestinal mucosal epithelial cells at day 4 and 7 compared to day 0 （24.93 ± 3.41%, 30.46 ± 5.24% vs 12.92 ± 4.20%, p 〈 0.01）. These changes were accompanied by the significant improvement of villus height （500.54 ± 53.79 um, 459.03 ± 88.98um vs 210.94 ± 49.16 um, P 〈 0.01）, serum levels of total proteins （70.52 ± 5.13 g/L, 74.89 ± 5.16 g/L vs 63.51 ± 2.47 g/L, P 〈 0.01）, albumin （39.44 ± 1.18 g/L, 42.39 ± 1.68 g/L vs 35.74 ± 1.75 g/L, P 〈 0.01） and fibronectin （236.3 4- 16.5 mg/L, 275.8± 16.9 mg/L vs 172.5 ± 21.4 mg/L, P 〈 0.01） at day 4 and 7, and prealbumin （286.38 ± 65.61 mg/L vs 180.88 ± 48.28 mg/L, P 〈 0.05）, transferrin （2.61 ± 0.12 g/L vs 2.41 ±0.14 g/L, P 〈 0.05） at day 7. Nitrogen excretion was significantly decreased at day 7 （3.40 ± 1.65 g/d vs 7.25 ± 3.92 g/d, P 〈 0.05）. No change was observed in the body weight. CONCLUSION： Recombinant human growth hormone could promote intestinal mucosal epithelial cell proliferation and protein synthesis in patients with enterocutaneous fistula.

关键词： Recombinant human growth hormone Enterocutaneous fistula Intestinal Epithelial cell Proliferating cell nuclear antigen

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Risk factors for leukopenia in patients with gastrointestinal fistula

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Chinese Medical Journal 2010年第23期123卷 3433-3437页

作者： ZHOU Zheng ren jian-an liU Hai-yan gu guo-sheng li jie-shou The Research Institute of General Surgery Jinling HospitalMedical School of Nanjing University Nanjing Jiangsu 210093China Intensive Care Unit First Affiliated Hospital of Anhui MedicalUniversity Hefei Anhui 230022 China

Background White blood cell count is an important index to the outcome of patients. In hospital, leukopenia is accompanied by high mortality, morbidity and treatment costs. However, in infectious diseases, the reasons responsible for leucopenia was not well elucidated. We investigated patients with gastrointestinal fistula to find risk factors for *** A prospective case control investigation was carried out in the Gastrointestinal Fistula Center, General Surgical Institute of Jinling Hospital. Cases included gastrointestinal fistula patients with leukopenia （n=98） and controls composed of gastrointestinal fistula patients with normal white blood cell count （n=78）. The two groups were compared for risk factors of leucopenia by statistical *** Factors associated with an increased risk for leukopenia included bacterial infection （25.5%） and hypoalbuminaemia （61.2%）. Multivariable Logistic regression analysis identified bacterial infection （80%）, urinary catheter （70%） and central vein catheter （60%） as the independent determinants for mortality in *** In patients with gastrointestinal fistula, two independent factors for leukopenia and three significant predictors of mortality were elucidated. We suggest that clinicians give patients more supportive management and apply prevention strategies to treat and prevent leukopenia.

关键词： risk factors leukopenia gastrointestinal fistula

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Polymorphism of heat shock protein 70-2 and enterocutaneous fistula in Chinese population

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World Journal of Gastroenterology 2014年第35期20卷 12559-12565页

作者： Jun Chen jian-An ren Gang Han guo-sheng gu Ge-Fei Wang Xiu-Wen Wu Bo Zhou Dong Hu Yin Wu Yun-Zhao Zhao jie-shou li Department of Surgery the Research Institute of General SurgeryJinling Hospital General Surgery Center of the Second Hospital of Jilin University Department of Surgery The Research Institute of General SurgeryJinling Hospital Medical School of Nanjing University

AIM: To investigate whether the heat shock protein 70-2 (HSP70-2) polymorphism is associated with enterocutaneous fistulas in a Chinese population.

关键词： Enterocutaneous fistulas Single nucleotide polymorphisms Heat shock protein HSP70-2

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paired Coagulation Status in the Crohn＇s Disease Patients Complicated with Intestinal Fistula

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Chinese Medical Journal 2018年第5期131卷 567-573页

作者： Yuan li jian-An ren Ge-Fei Wang guo-sheng gu Xiu-Wen Wu Song liu Hua-jian ren Zhi-Wu Hong jie-shou li Department of General Surgery Jinling Hospital Medical School of Nanjing University Nanjing Jiangsu 210002 China Department of General Surgery Nanjing Drum Tower Hospital The Affiliated Hospital of Nanjing University Medical School Nanjing Jiangsu 210093 China

Background： Intestinal fistula is one of the common complications of Crohn＇s disease （CD） that might require surgical treatment. The clinical characteristics and outcomes of CD with intestinal fistula are much different from CD alone. This study was to investigate whether the coagulation status of CD is changed by intestinal fistula. Methods： Data were retrospectively analyzed for 190 patients with a definitive diagnosis of CD who were registered at the Jinting Hospital between January 2014 and September 2015. Baseline clinical characteristics and laboratory indices of initial admission and 7 days after intestinal fistula resections were collected. Student＇s t-test and the Wilcoxon rank-sum test were used to compare differences between the two groups. Results： Compared with CD patients without intestinal fistula, prothrombin time （PT） in patients with intestinal fistula was significantly longer （12.13 ± 1.27 s vs. 13.18± 1.51 s,P〈0.001 in overall cohort： 11.56± 1.21 svs. 12.61 ±0.73 s,P 0.001 in females： and 12.51± 1.17 s vs. 13.37± 1.66 s, P - 0.003 in males）. Platelet （PLT） count was much lower in intestinal fistula group than in nonintestinal fistula group （262.53 ± 94.36 × 109/L vs. 310.36 ± 131.91× 109/L, P = 0.009）. Multivariate logistic regression showed that intestinal fistula was significantly associated with a prolonged PT （odds ratio [OR] 1.900, P 〈 0.001 ）, a reduced amount of PLT （OR = 0.996, P = 0.（124）, and an increased operation history （OR = 5.408, P 〈 0.001 ）. Among 65 CD patients receiving intestinal fistula resections, PT was obviously shorter after operation than baseline （ 12.28 ± 1.16 s vs. 13.02 ± 1.64 s, P = 0.006）. Conclusions： Intestinal fistula was significantly associated with impaired coagulation status in patients complicated with CD. Coagulation status could be improved after intestinal fistula resections.

关键词： Coagulation Crohn's Disease: Inflammatory Bowel Disease: Intestinal Fistula Prothrombin Time

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冬虫夏草对肠外瘘病人肠黏膜屏障功能的影响

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肠外与肠内营养 2015年第2期22卷 71-75,78页

作者：顾国胜任建安李宁黎介寿南京军区南京总医院解放军普通外科研究所江苏南京210002

目的:探讨冬虫夏草对肠外瘘病人肠黏膜屏障功能的影响。方法:选择肠外瘘病人17例,随机分为肠内营养(EN)组8例和EN+百令胶囊组9例。入院次日,病人给予1.5 REE(静息能量消耗)热量的EN液,研究组增给百令胶囊鼻饲,每天3 g,连用10 d。营养支... 详细信息

目的:探讨冬虫夏草对肠外瘘病人肠黏膜屏障功能的影响。方法:选择肠外瘘病人17例,随机分为肠内营养(EN)组8例和EN+百令胶囊组9例。入院次日,病人给予1.5 REE(静息能量消耗)热量的EN液,研究组增给百令胶囊鼻饲,每天3 g,连用10 d。营养支持与方法在整个研究期间不变,分别于研究的第0、5、10天经小肠瘘口取肠黏膜标本,检测小肠黏膜细胞增殖指数、免疫组化标记增殖细胞核抗原、流式细胞仪计数IEL(CD3、CD45双标记),并分析其亚型CD4和CD8阳性细胞比例。结果:EN组和EN+百令胶囊组病人的的小肠黏膜细胞增殖指数及增殖细胞核抗原的比例在第10天时与0天比有显著性差异(P<0.05)。两组肠上皮内淋巴细胞比例及其亚型第5天即出现明显变化(P<0.05)。结论:冬虫夏草不仅可促进肠瘘病人肠黏膜细胞的增殖,而且还能增强肠黏膜的免疫屏障功能。

关键词：冬虫夏草黏膜屏障增殖指数增殖细胞核抗原

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冬虫夏草对大鼠小肠黏膜机械屏障功能影响的研究

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肠外与肠内营养 2010年第6期17卷 359-362页

作者：顾国胜任建安李宁黎介寿南京大学医学院临床学院(南京军区南京总医院)解放军普通外科研究所江苏南京210002

目的:探讨冬虫夏草对大鼠小肠黏膜机械屏障功能的影响。方法:实验分为正常对照组、内毒素灌胃组和内毒素灌胃+冬虫夏草组。内毒素灌胃组于实验开始第1天给予内毒素5 EU/kg灌胃,内毒素灌胃+冬虫夏草组每天给予冬虫夏草5 g/(kg.d)混悬液灌... 详细信息

目的:探讨冬虫夏草对大鼠小肠黏膜机械屏障功能的影响。方法:实验分为正常对照组、内毒素灌胃组和内毒素灌胃+冬虫夏草组。内毒素灌胃组于实验开始第1天给予内毒素5 EU/kg灌胃,内毒素灌胃+冬虫夏草组每天给予冬虫夏草5 g/(kg.d)混悬液灌胃,对照组给予等量的等渗盐水灌胃,三组均自由进食和饮水。分别于灌胃第4、7、10天取8只大鼠,取小肠黏膜行流式细胞仪检测黏膜细胞的增殖指数(PI),透射电镜检测黏膜上皮细胞间紧密连接宽度,免疫组化染色检测黏膜增殖细胞核抗原(PCNA)。结果:内毒素灌胃+冬虫夏草组大鼠肠黏膜细胞的PI、PCNA比例和肠黏膜上皮细胞间的紧密连接第7天即恢复至正常水平,与内毒素灌胃组相比,有显著性差异。结论:冬虫夏草可促进大鼠小肠黏膜细胞的增殖,维持小肠黏膜上皮细胞间紧密连接的完整性,保护肠黏膜的机械屏障功能。

关键词：冬虫夏草机械屏障紧密连接增殖细胞核抗原增殖指数

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肠内营养对长期禁食肠瘘患者肠黏膜上皮内淋巴细胞及黏液屏障功能的研究

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中华胃肠外科杂志 2006年第6期9卷 527-529页

作者：洪书剑顾国胜任建安李宁黎介寿南京大学医学院临床学院南京军区南京总医院普通外科全军普通外科研究所 210002

目的研究肠内营养对长期禁食的肠外瘘患者肠黏膜上皮内淋巴细胞(iIEL)及黏液屏障功能的影响。方法对2003年7月至2004年5月收治的10例唇状小肠外瘘病例,分别于实施肠内营养支持前和实施第5天及第10天,在肠镜下经小肠外瘘口距瘘口15～20 c... 详细信息

目的研究肠内营养对长期禁食的肠外瘘患者肠黏膜上皮内淋巴细胞(iIEL)及黏液屏障功能的影响。方法对2003年7月至2004年5月收治的10例唇状小肠外瘘病例,分别于实施肠内营养支持前和实施第5天及第10天,在肠镜下经小肠外瘘口距瘘口15～20 cm以活检钳取检肠黏膜,行常规苏木精-伊红染色计数iIEL,免疫组织化学染色测定CD8阳性淋巴细胞比例和黏蛋白基因2阳性细胞比例,特殊染色阿利新蓝(Alcian Blue,AB)结合法检测黏液层厚度。结果肠内营养实施5d后,各活检标本黏蛋白基因2阳性细胞比例及黏液层厚度较实施前显著增高(P＜0．05)；10 d后,iIEL及CD8阳性淋巴细胞比例也较肠内营养实施前显著增多(P＜0．05)。结论实施肠内营养对于长期禁食的肠外瘘患者的肠道黏膜具有保护作用,并可增强肠黏膜的免疫功能。

关键词：肠黏膜上皮内淋巴细胞唇状,肠瘘黏蛋白基因黏液屏障

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SAPT:a synchrotron-based proton therapy facility in Shanghai

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Nuclear Science and Techniques 2023年第10期34卷 36-46页

作者： Man-Zhou Zhang De-Ming li li-ren Shen Hai-Rong Zhang Zhi-ling Chen Han-Wen Du Ming gu Rui li De-Kang liu Yue-Hu Pu Jun-Feng Yu jian-Feng Chen Chu Chen Chun-Long guo Hao guo Ge-Yang jiang Zhi-Qiang jiang lin Jin Wen-Jing li Xiu-Fang li Ye lin Ming liu Yong-Hao liu Ya-Juan liu Ming Lv Qing-Ru Mi lian-Hua Ouyang Wei-guo Shi Hang Shu Qi-sheng Tang Kun Wang Zhi-Shan Wang Jun Wu Xiao-Bing Wu Jia-Qiang Xu Wen-Zhen Xu Chong-Xian Yin Cheng Yu ren-Xian Yuan Qi-Bing Yuan Hai-Qun Zhang Miao Zhang Wen-Zhi Zhang li-Ying Zhao Wei-Min Zhou shou-Xian Fang Xi-Dong Sun Zhen-Tang Zhao Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China Shanghai Advanced Research Institute Chinese Academy of SciencesShanghai 201204China APACTRON Particle Equipment Co. Ltd.Shanghai 201815China The Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China

Because of its excellent dose distribution,proton therapy is becoming increasingly popular in the medical application of cancer treatment.A synchrotron-based proton therapy facility was designed and constructed in *** synchrotron,beam delivery system,and other technical systems were commissioned and reached their expected *** a clinical trial of 47 patients was finished,the proton therapy facility obtained a registration certificate from the National Medical Products *** characteristics of the accelerator and treatment systems are described in this article.

关键词： Proton therapy Synchrotron Slow extraction Gantry Beam delivery system

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Efficacy and safety of sirolimus early conversion protocol in liver transplant patients with hepatocellular carcinoma:A single-arm, multicenter, prospective study

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Hepatobiliary & Pancreatic Diseases International 2022年第2期21卷 106-112页

作者： ren-Yi Su Sun-Bin ling Qiao-Nan Shan Xu-Yong Wei Rui Wang Chang-Ku Jia li Zhuang Tian Shen li-Min Ding Zhi-Dan Xu Lai-Bang Luo li-Bo Sun guang-Ming li Tai-Shi Fang Nan jiang Kun Zhang Zhao-jie Su Zhi-Hai Peng ren Lang Tao jiang Qiang He lin-Sen Ye Yang Yang Yu-Ting He Wen-Zhi guo liu-Gen Lan Xu-Yong Sun Dong Chen Zhi-Shui Chen Da-Wei Zhou Shao-Jun Ye Qi-Fa Ye Min Tian jian-Hua Shi Bo Wang jiang liu Qian Lu Wei Rao Jin-Zhen Cai Tao Lv Jia-Yin Yang Pu-Sen Wang lin Zhong Jing-sheng Ma Qi-Gen li sheng-Dong Wu Chang-jiang Lu Cai-De Lu Dong-Hua Zhang Xuan Wang Zi-Qiang li Mu-jian Teng Jun-jie li Wen-Tao jiang jian-Hua li Quan-Bao Zhang ning-Qi Zhu Zheng-Xin Wang Kang He Qiang Xia Shao-Hua Song Zhi-ren Fu Wei Qiu guo-Yue Lv Rui-Peng Song Ji-Zhou Wang Zheng Wang jian Zhou Gang Chen Ying-Peng Zhao li li Ze-Min Hu Qi-jie Luo Zhong-Zhou Si Bin Xie Xiao-Shun He Zhi-Yong guo Shu-Sen Zheng Xiao Xu Department of Hepatobiliary and Pancreatic Surgery The Center for Integrated Oncology and Precision MedicineAffiliated Hangzhou First People’s HospitalZhejiang University School of MedicineHangzhou 310006China NHC Key Laboratory of Combined Multi-organ Transplantation Hangzhou 310003China Department of Hepatobiliary and Pancreatic Surgery Shulan(Hangzhou)HospitalZhejiang Shuren University School of MedicineHangzhou 310022China Department of Hepatobiliary and Pancreatic Surgery The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China Department of Transplantation Jiangxi Provincial People’s Hospital Affiliated to Nanchang UniversityNanchang 330006China liver Transplantation Center Beijing You’an HospitalCapital Medical UniversityBeijing 100069China Department of Hepatic Surgery National Clinical Research Center for Infectious DiseaseShenzhen Third People’s HospitalThe Second Affiliated Hospital of Southern University of Science and TechnologyShenzhen 518112China Department of General Surgery Xiang’an Hospital of Xiamen UniversityXiamen 361000China Deartment of Hepatobiliary and Pancreaticosplenic Surgery Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China Department of Hepatic Surgery and liver Transplantation Center The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510630China Department of Hepatobiliary and Pancreatic Surgery The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China Department of liver Transplantation The Second Affiliated Hospital of Guangxi Medical UniversityNanning 530000China Institute of Organ Transplantation Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Institute of Hepatobiliary Diseases of Wuhan University Transplant Center of Wuhan UniversityHubei Key Laboratory of Medical Technology on TransplantationZhongnan Hospital of Wuhan UniversityWuhan 430071China Department of Hepatobiliary Surgery The First Affiliated Hos

Mammalian target of rapamycin(m TOR) inhibitor as an attractive drug target with promising antitumor effects has been widely investigated. High quality clinical trial has been conducted in liver transplant(LT) recipients in Western countries. However, the pertinent studies in Eastern world are paucity. Therefore, we designed a clinical trial to test whether sirolimus can improve recurrence-free survival(RFS) in hepatocellular carcinoma(HCC) patients beyond the Milan criteria after LT. This is an open-labeled, single-arm, prospective, multicenter, and real-world study aiming to evaluate the clinical outcomes of early switch to sirolimus-based regimens in HCC patients after LT. Patients with a histologically proven HCC and beyond the Milan criteria will be enrolled. The initial immunosuppressant regimens are center-specifc for the frst 4-6 weeks. The following regimens integrated sirolimus into the regimens as a combination therapy with reduced calcineurin inhibitors based on the condition of patients and centers. The study is planned for 4 years in total with a 2-year enrollment period and a 2-year follow-up. We predict that sirolimus conversion regimen will provide survival benefts for patients particular in the key indicator RFS as well as better quality of life. If the trial is conducted successfully, we will have a continued monitoring over a longer follow-up time to estimate indicator of overall survival. We hope that the outcome will provide better evidence for clinical decision-making and revising treatment guidelines based on Chinese population data.

关键词： Sirolimus Immunosuppressive agents Hepatocellular carcinoma liver transplantation Conversion

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In-beam gamma rays of CSNS Back-n characterized by black resonance filter

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Nuclear Science and Techniques 2024年第10期35卷 149-160页

作者： Jin-Cheng Wang jie ren Wei jiang Xi-Chao Ruan Ying-Yi liu Hao-Lan Yang Kuo-Zhi Xu Xin-Yi Pan Qi Sun jie Bao Han-Xiong Huang Hao-Fan Bai jiang-Bo Bai Ping Cao Qi-Ping Chen Yong-Hao Chen Wen-Hao Duan An-Chuan Fan Rui-Rui Fan Chang-Qing Feng Min-Hao gu Chang-Cai Han Zi-jie Han guo-Zhu He Yong-Cheng He Yang Hong Yi-Wei Hu Zhi-jie jiang ling Kang Chang-lin Lan Bo li Feng li Qiang li Xiao li Yang li jie liu Rong liu Shu-Bin liu Yi-Na liu guang-Yuan Luan Chang-Jun ning Yi-Jia Qiu Wen-Kai ren Zhi-Zhou ren Zhao-Hui Song Kang Sun Zhi-Xin Tan Jing-Yu Tang sheng-Da Tang li-Jiao Wang Peng-Cheng Wang Zhao-Hui Wang Zhong-Wei Wen Xiao-guang Wu Xuan Wu Ze-Peng Wu Cong Xia li-Kun Xie Han Yi Tao Yu Yong-Ji Yu guo-Hui Zhang Hang-Chang Zhang Qi-Wei Zhang Xian-Peng Zhang Yu-liang Zhang Zhi-Yong Zhang Mao-Yuan Zhao Zhi-Hao Zhou Ke-Jun Zhu Chong Zou Key Laboratory of Nuclear Data China Institute of Atomic EnergyBeijing 102413China Institute of High Energy Physics Chinese Academy of Sciences(CAS)Beijing 100049China Spallation Neutron Source Science Center Dongguan 523803China State Key Laboratory of Nuclear Physics and Technology School of PhysicsPeking UniversityBeijing 100871China Institute of Modern Physics Fudan UniversityShanghai 200433China State Key Laboratory of Particle Detection and Electronics University of Science and Technology of ChinaHefei 230026China School of Nuclear Science and Technology University of Science and Technology of ChinaHefei 230026China Institute of Nuclear Physics and Chemistry China Academy of Engineering PhysicsMianyang 621900China Department of Modern Physics University of Science and Technology of ChinaHefei 230026China USTC Archaeometry Lab University of Science and Technology of ChinaHefei 230026China State Key Laboratory of Particle Detection and Electronics Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China Northwest Institute of Nuclear Technology Xi’an 710024China University of Chinese Academy of Sciences Beijing 100049China School of Nuclear Science and Technology Lanzhou UniversityLanzhou 730000China Institute of Advanced Technology University of Science and Technology of ChinaHefei 230027China

The back-streaming white-neutron beamline(Back-n)of the China Spallation Neutron Source is an essential neutronresearch platform built for the study of nuclear data,neutron physics,and neutron *** types of cross-sectional neutron-reaction measurements have been performed at Back-n since early *** measurements have shown that a significant number of gamma rays can be transmitted to the experimental stations of Back-n along with the neutron *** gamma rays,commonly referred to as in-beam gamma rays,can induce a non-negligible experimental background in neutron-reaction *** the characteristics of in-beam gamma rays is important for understanding the experimental ***,measuring in-beam gamma rays is challenging because most gamma-ray detectors are sensitive to neutrons;thus,discriminating between neutron-induced signals and those from in-beam gamma rays is *** this study,we propose the use of the black resonance filter method and a CeBr_(3) scintillation detector to measure the characteristics of the in-beam gamma rays of *** types of black resonance filters,^(181)Ta,^(59)Co,^(nat)Ag,and^(nat)Cd,were used in this *** time-of-flight(TOF)technique was used to select the detector signals remaining in the absorption region of the TOF spectra,which were mainly induced by in-beam gamma *** energy distribution and flux of the in-beam gamma rays of Back-n were determined by analyzing the deposited energy spectra of the CeBr_(3) scintillation detector and using Monte Carlo *** on the results of this study,the background contributions from in-beam gamma rays in neutron-reaction measurements at Back-n can be reasonably evaluated,which is beneficial for enhancing both the experimental methodology and data analysis.

关键词： In-beam gamma rays Back-n CeBr_(3)scintillator Black filter resonance technique

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