检索结果-南通市图书馆

Single-tube-genotyping of gastric cancer related SNPs by directly using whole blood and paper-dried blood as starting materials

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2006年第24期12卷 3814-3820页

作者： huan huang Ying Bu guo-hua zhou Medical School Nanjing University Nanjing 210093 China and China Pharmaceutical University Nanjing 210009 China huadong Research Institute for Medicine and Biotechnics Nanjing 210002 China

AIM: To demonstrate an inexpensive method for typing gastric cancer related single nucleotide polymorphisms (SNPs) using whole blood or paper-dried blood as starting materials. METHODS: PCR amplification is directly carried out from the whole blood or paper-dried blood sample without any DNA extraction step. Before PCR, a blood sample, four primers, and all of biological reagents necessary for PCR were added at a time; After PCR, the amplified products were directly separated by slab gel electrophoresis or microchip CE without any purification. SNP typing was performed by tetra-primer PCR with two inner primers specific to each allele and two outer primers defining the length of allele-specific amplicons. Genotypes were directly discriminated by the size of amplicons specific to each allele, thereby avoiding any post-PCR process. RESULTS: Using a special PCR buffer, inhibitory substances in blood (including the anticoagulant in blood) and filter paper were effectively suppressed; a 'true' single-tube-genotyping is thus realized. We successfully determined genotypes IL-1B-511 and IL-1B-31 polymorphisms at the gene IL-1B by using whole-blood and paper-dried blood samples as starting materials respectively. The method is so sensitive that 0.5-1.0μL of blood sample is enough to give a satisfactory typing results. The genotyping results were confirmed by RFLP-PCR using purified genome DNA, indicating that amplification specificity was not affected by inhibitory components (including coagulants) in blood or filter paper. CONCLUSION: Compared with SNP typing methods based on purified DNA, the proposed method is labor saving, simple, inexpensive, and less cross-contaminated. It is promising to use this method to type other SNPs.

关键词： Gastric cancer Whole blood Paper-driedblood Tetra-PCR SNP IL-1B-31 IL-1B-511

An active reflector antenna using a laser angle metrology system

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Research in Astronomy and Astrophysics 2012年第6期12卷 713-722页

作者： Yong Zhang Jie Zhang De-hua Yang guo-hua zhou Ai-hua Li guo-Ping Li National Astronomical Observatories/Nanjing Institute of Astronomical Optics ＆ Technology Chinese Academy of Sciences Nanjing 210042 China Key Laboratory of Astronomical Optics ＆ Technology Nanjing Institute of Astronomical Optics＆ Technology Chinese Academy of Sciences Nanjing 210042 China Graduate University of Chinese Academy of Sciences Beijing 100049 China

An active reflector is one of the key technologies for constructing large telescopes, especially for millimeter/sub-millimeter radio telescopes. This article in- troduces a new efficient laser angle metrology system for an active reflector antenna on large radio telescopes. Our experiments concentrate on developing an active reflec- tor for improving the detection precisions and the maintenance of the surface shape in real time on the 65-meter prototype radio telescope constructed by Nanjing Institute of Astronomical Optics and Technology （NIAOT; http：//***/）. The test results indicate that the accuracy of the surface shape segmentation and maintenance has the dimensions of microns, and the time-response can be on the order of minutes. Our efforts proved to be workable for sub-millimeter radio telescopes.

关键词： radio radiation -- telescopes -- adaptive optics -- sub-millimeter

ZM1 magnesium alloy reinforced by carbon nanotubes using an improved casting process

在线全文

学校读者我要写书评

暂无评论

Rare Metals 2021年第5期40卷 1275-1283页

作者： Qiu-Hong Yuan Lin Liao guo-hua zhou Zheng-Yun Liu Physical Science and Technology College Yichun UniversityYichun 336000China Department of Materials Processing Engineering Nanchang UniversityNanchang 330031China

Melt processing is a promising process to fabricate Mg-CNTs(carbon nanotubes)composites with high performance and large *** this work,the liquidmixing technique and ball-milling processes as well as the stirring casting method were used to prepare ZM1-1.0 CNTs-L and ZM1-1.0 CNTs-B composites,*** composites were studied using optical microscopy(OM),Raman spectroscopy,scanning electron microscope(SEM),transmission electron microscope(TEM)and room-temperature tensile *** results show that the yield strength(YS),ultimate tensile strength(UTS),elongation and microhardness values of the ***-L composite are improved by 37.3%,29.6%,37.0%and33.3%,respectively,compared with those of ZM1 *** uniform dispersion of CNTs as well as the strong interfacial bonds with the Mg matrix is the main reason for the significant improvement in mechanical properties of the as-cast *** mixing combined with the stirring casting method is favorable to fabricate Mg-CNTs composite with high mechanical properties.

关键词： ZM1 alloy Carbon nanotubes(CNTs) Composites Microstructure Mechanical properties

An Alternative Low-Cost Strategy for Simultaneous Sensitive Detection of Adjacent ESR1 Mutations in Single Circulating Tumor Cell

在线全文

学校读者我要写书评

暂无评论

Journal of Analysis and Testing 2023年第1期7卷 89-100页

作者： Chen Wang Jing-Jie Wang Xi-Ru Xue Yi-Yun Shen Bing-Jie Zou Shao-hua Wang Qin-Xin Song guo-hua zhou Key Laboratory of Drug Quality Control and Pharmacovigilance Ministry of EducationSchool of PharmacyChina Pharmaceutical UniversityNanjing 210009China Department of General Surgery Jinling HospitalMedical School of Nanjing UniversityNanjing 210002China Department of Clinical Pharmacy State Key Laboratory of Analytical Chemistry for Life Science&Jiangsu Key Laboratory of Molecular MedicineJinling HospitalMedical School of Nanjing UniversityNanjing 210002China The First School of Clinical Medicine Southern Medical UniversityGuangzhou 510515China

ESR1 mutation is of great clinical significance and being promoted as a marker of resistance to endocrine therapy in breast ***,it is a challenging task to detect ESR1 mutations from traditional biopsies and cell-free DNA(cfDNA),especially for polyclonal *** is mainly attributed to massive wild-type background and the low-abundance of the ***,using one-step single-cell amplification coupling with pyrosequencing,we developed and validated an original strategy for simultaneous sensitive detection of adjacent ESR1 mutations in single circulating tumor cell(CTC)from breast *** expensive single-cell sequencing used in previous studies,the strategy does not require complicated two-step amplification or high-cost single-cell amplification *** pivotal parts involved in the strategy are collection of CTCs from whole blood of breast cancer patients,one-step single-cell amplification and pyrosequencing of single-cell *** achieve better e fficiency of one-step single-cell amplification for pyrosequencing,a set of experimental conditions were thoroughly *** developed strategy enabled a highly specific detection of the ESR1 hotspot mutations from large wild-type background with the specificity as low as 1%and a high sensitivity of two copies of artificial samples or single-cell level and pretty good quantitative accuracy(R^(2)≥0.9888).Using this strategy,141 single CTCs from 11 cases of breast cancer patients were identified and collected,126 of which were successfully analyzed with a high rate of 89.4%.These results indicate that the cost-effective and reliable strategy could be used for clinical management,showing promising application in the treatment decision-making of breast cancer patients.

关键词： ESR1 mutation Breast cancer Acquired resistance Single-cell amplification Pyrosequencing

Deep genome skimming reveals the hybrid origin of Pseudosasa gracilis (Poaceae: Bambusoideae)

在线全文

学校读者我要写书评

暂无评论

Plant Diversity 2024年第3期46卷 344-352页

作者： Xiang-zhou Hu Cen guo Sheng-Yuan Qin De-Zhu Li Zhen-hua guo Germplasm Bank of Wild Species Kunming Institute of BotanyChinese Academy of SciencesKunmingYunnan 650201China University of Chinese Academy of Sciences Beijing 100049China

Pseudosasa gracilis(Poaceae:Bambusoideae)is a temperate woody bamboo species endemic to Southcentral China with a narrow *** phylogenetic studies revealed an unexpected,isolated phylogenetic position of *** we conducted phylogenomic analysis by sampling populations of *** and its sympatric species *** and Sinosasa polytricha reflecting different genomic signals,by deep genome *** molecular evidence from chloroplast genes and genome-wide SNPs,we deciphered the phylogenetic relationships of *** plastid and nuclear data indicate that *** is more closely related to Sinosasa,which is discordant with the taxonomic *** further explore this molecular-morphological conflict,we screened 411“perfect-copy”syntenic genes to reconstruct phylogenies using both the concatenation and coalescent *** observed extensive discordance between gene trees and the putative species tree.A significant hybridization event was detected based on 411 genes from the D subgenome,showing *** was a hybrid descendant between Sinosasa longiligulata and ***,with 63.56%and 36.44%inheritance probabilities of each ***,introgression events were detected in the C subgenome between *** and *** in the same distribution *** findings suggest that sympatric hybridization and introgression play a crucial role in the origin of *** providing an empirical example of bamboo of hybrid origin using comprehensive analyses based on genomic data from different inheritance systems and morphological characters,our study represents a step forward in understanding of reticulate evolution of bamboos.

关键词： Phylogenomics Hybridization Introgression Pseudosasa gracilis Pseudosasa Sinosasa

The E3 ubiquitin ligase TRAF6 controls CTLA-4 turnover and promotes T-cell-mediated antitumor immunity

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2024年第1期21卷 97-99页

作者： Xiao-Liang Jie hua guo Guang-Biao zhou State Key Laboratory of Molecular Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical College100021 BejingChina

Yu et al.[1]reported that the tumor necrosis factor(TNF)receptor OX40(CD134)-TNF receptor-associated factor 6(TRAF6)axis mediates Lys63-linked ubiquitination and subsequent lysosomal degradation of cytotoxic T-lymphocyte antigen 4(CTLA-4),leading to the enhancement of CD8^(+)T-cell-mediated antitumor *** findings unveil a previously unknown mechanism in controlling the stability of the immune checkpoint CTLA-4 and provide a potential therapeutic target for the improvement of Tcell-based immunotherapy.

关键词： immunity OX40 ubiquitin

Panel positioning error and support mechanism for a 30-m THz radio telescope

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Research in Astronomy and Astrophysics 2011年第6期11卷 725-736页

作者： De-hua Yang Daniel Okoh guo-hua zhou Ai-hua Li guo-Ping Jing-Quan Cheng National Astronomical Observatories /Nanjing Institute of Astronomical Optics ＆ Technology Chinese Academy of SciencesNanjing 210042China Key Laboratory of Astronomical Optics ＆ Technology Nanjing Institute of Astronomical Optics ＆ TechnologyChinese Academy of SciencesNanjing 210042China Centre for Basic Space Science National Space Research and Development AgencyUniversity of NigeriaNsukka 410002Nigeria National Radio Astronomy Observatory CharlottesvilleVA 22903USA

A 30-m TeraHertz（THz） radio telescope is proposed to operate at 200 μm with an active primary *** paper presents sensitivity analysis of active surface panel positioning errors with optical performance in terms of the Strehl *** on Ruze＇s surface error theory and using a Monte Carlo simulation,the effects of six rigid panel positioning errors,such as piston,tip,tilt,radial,azimuthal and twist displacements,were directly *** optical performance of the telescope was then evaluated using the standard Strehl *** graphically illustrated the various panel error effects by presenting simulations of complete ensembles of full reflector surface errors for the six different rigid panel positioning *** of the panel error sensitivity analysis revealed that the piston error and tilt/tip errors are dominant while the other rigid errors are much less ***,as indicated by the results,we conceived of an alternative Master-Slave Concept-based（MSC-based） active surface by implementating a special Series-Parallel Concept-based（SPC-based） hexapod as the active panel support mechanism.A new 30-m active reflector based on the two concepts was demonstrated to achieve correction for all the six rigid panel positioning errors in an economically feasible way.

关键词： telescopes-methods:data analysis-methods:statistical-techniques:miscellaneous

Role of alloying and heat treatment on microstructure and mechanical properties of cast Al-Li alloys:A review

在线全文

学校读者我要写书评

暂无评论

China Foundry 2024年第5期21卷 445-460页

作者： guo-hua Wu You-jie guo Fang-zhou Qi Shen Zhang Yi-xiao Wang Xin Tong Liang Zhang School of Materials Science and Engineering Shanghai Jiao Tong UniversityShanghai 200240China

Due to the prominent advantages of low density,high elastic modulus,high specific strength and specific stiffness,cast Al-Li alloys are suitable metallic materials for manufacturing complex large-sized components and are ideal structural materials for aerospace,defense and military *** the basis of the microstructural characteristics of cast Al-Li alloys,exploring the role of alloying and micro-alloying can stabilize their dominant position and further expand their application *** this review,the development progress of cast Al-Li alloys was summarized *** to the latest research highlights,the influence of alloying and heat treatment on the microstructure and mechanical properties was systematically *** potential methods to improve the alloy performance were *** response to the practical engineering requirements of cast Al-Li alloys,the scientific challenges and future research directions were discussed and prospected.

关键词： cast Al-Li alloy alloying microstructure mechanical properties heat treatment

Updates of developments in interventional therapy for elderly patients with cardiovascular diseases

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Geriatric Cardiology 2024年第1期21卷 1-3页

作者： hua SHEN Xun-Xun FENG Qian-Yun guo Yu-Jie zhou Beijing Key Laboratory of Precision Medicine of Coronary Atherosclerotic Disease Beijing Institute of Heart Lung and Blood Vessel DiseaseClinical Center for Coronary Heart DiseaseDepartment of CardiologyBeijing Anzhen HospitalCapital Medical UniversityBeijingChina

Cardiovascular diseases(CVD)are the leading cause of death in the world and one of the most common diseases in the elderly,with high prevalence and poor prognosis,which seriously affect the health of the elder *** report showed that global deaths from CVD increased from 12.4 million in 1990 to 19.8 million in 2022,reflecting the global population growth and aging,as well as the impact of metabolic,environmental,and behavioral risks.[1]With the progress of aging population,the burden of CVD in the elderly in China has increased year by year,and factors including multiple complications,organ function decline,and high complication rate have made the interventional treatment of CVD in the elderly one of the difficulties and focuses in the field of cardiology in China.

关键词： diseases patients prognosis