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KISS-1 inhibits the proliferation and invasion of gastric carcinoma cells

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World Journal of Gastroenterology 2012年第15期18卷 1827-1833页

作者： Na Li Hong-Xing Wang Jie Zhang Ya-Ping Ye guo-yang he Department of Pathology Xinxiang Medical University Xinxiang 453003 Henan Province China Department of Clinical Immunology Xinxiang Medical University Xinxiang 453003 Henan Province China

AIM:To investigate the function of the KISS-1 gene in gastric carcinoma cells and to explore its potential ***:A KISS-1 eukaryotic expression vector was constructed and transfected into BGC-823 *** clones were obtained through G418 *** transcription-polymerase chain reaction and western blotting were used to detect KISS-1 and matrix metalloproteinase-9(MMP-9)expression in transfected *** growth of transfected cells was investigated by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide(MTT)proliferation assays,and the cells'invasive potential was analyzed by basement membrane(Matrigel)invasion *** anti-tumor effects of KISS-1 were tested in vivo using allografts in nude ***:The expression level of KISS-1 mRNA andprotein in BGC-823/KISS-1 transfected cells were significantly higher than in BGC-823/pcDNA3.1 transfected cells(Phe parental BGC-823 cell line(Phe expression level of MMP-9 mRNA and protein in BGC-823/KISS-1 were significantly less than in BGC-823/pcDNA3.1(Phe proliferation of BGC-823/KISS-1 cells at 48 h(0.642±0.130)and 72 h(0.530±0.164)were significantly reduced compared to BGC-823/pcDNA3.1(0.750±0.163,0.645±0.140)(Phe invasive potential of BGC-823/KISS-1 cells(16.50±14.88)is significantly reduced compared to BGC-823/pcDNA3.1(20.22±14.87)(Phe rate of pcDNA3.1-KISS-1 tumor growth is significantly slower than pcDNA3.1 and control cell tumor growth in nude ***,tumor volume of pcDNA3.1-KISS-1 tumors(939.38±82.08 mm3)was significantly less than pcDNA3.1(1250.46±44.36 mm3) and control tumors(1284.36±55.26 mm3)(Phe tumor mass of pcDNA3.1-KISS-1 tumors(0.494±0.84 g)was significantly less than pcDNA3.1(0.668±0.55 g)and control

关键词： KISS-1 Matrix metalloproteinase-9 BGC-823 cells Proliferation Metastasis Nude mice

基于移动高斯热源的激光抛光瞬态有限元三维数值模型

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激光与红外 2021年第5期51卷 575-583页

作者：贺国阳王涛河北工业大学机械工程学院天津300132

本研究模拟CO_(2)连续激光器抛光Ti6Al4V材料的工艺过程,利用可移动的高斯热源模拟激光源,建立有限元三位数值瞬态模型进行模拟实验,来模拟TC4材料表面轮廓的融化与凝固过程,该数值模型耦合了温度场与速度场,研究由于表面张力温度系数... 详细信息

本研究模拟CO_(2)连续激光器抛光Ti6Al4V材料的工艺过程,利用可移动的高斯热源模拟激光源,建立有限元三位数值瞬态模型进行模拟实验,来模拟TC4材料表面轮廓的融化与凝固过程,该数值模型耦合了温度场与速度场,研究由于表面张力温度系数而产生的表面张力对抛光熔池的影响,通过改变高斯光源的不同功率强度、扫描速度,来分析对抛光过程中形成的熔池的影响,模拟结果表明:当激光热源功率过大时,易造成熔池尺寸过大,不利于降低材料表面的粗糙度,适当降低扫描速度能有效的降低轮廓峰值的温度梯度大小,从而降低表面张力的作用大小,进而改善熔池尺寸与形状。通过实际抛光实验得出:激光的最佳扫描速度维持在20 mm/s左右,扫描速度过小或者过大都会使抛光样品的表面粗糙度值[WTBX]Ra增加,从而达不到理想抛光效果,且通过实验测试值与数值模型计算值相比较,发现两者数据大致吻合,从而证明了该数学模型的准确性。

关键词：激光抛光高斯热源熔池温度场表面张力温度系数

脉冲光纤激光抛光TC4合金的实验研究与抛光层微观形貌分析

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激光与红外 2021年第6期51卷 752-759页

作者：贺国阳王涛河北工业大学机械工程学院天津300312

针对Ti6Al4V材料的激光抛光工艺进行实验研究,通过改变脉冲式激光的工艺参数设置,来分析激光参数对抛光工艺效果的影响,主要研究了激光输出功率、离焦量e和激光光斑重叠率δ这三个参数的影响规律与作用机理,并结合试样表面粗糙度测量值... 详细信息

针对Ti6Al4V材料的激光抛光工艺进行实验研究,通过改变脉冲式激光的工艺参数设置,来分析激光参数对抛光工艺效果的影响,主要研究了激光输出功率、离焦量e和激光光斑重叠率δ这三个参数的影响规律与作用机理,并结合试样表面粗糙度测量值Ra,得出:激光输出功率为300 W左右,离焦量e控制在6~8 mm范围内,光斑重叠率δ在70%~75%范围内时,脉冲式激光对TC4合金材料表面的抛光效果最佳;最后通过光学显微镜测量仪器来分析抛光后的材料表面形貌与材料抛光层的内部晶格晶粒变化,来分析激光抛光对试样抛光层物理性质的影响,并对比抛光前后试样表面的进行了硬度测试对比。

关键词：激光抛光工艺参数微观形貌表面粗糙度

Low‑Temperature Oxidation Induced Phase Evolution with Gradient Magnetic heterointerfaces for Superior Electromagnetic Wave Absorption

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Nano-Micro Letters 2025年第1期17卷 191-204页

作者： Zizhuang he Lingzi Shi Ran Sun Lianfei Ding Mukun he Jiaming Li Hua guo Tiande Gao Panbo Liu School of Chemistry and Chemical Engineering Northwestern Polytechnical UniversityXi’an 710129People’s Republic of China School of Marine Science and Technology Northwestern Polytechnical UniversityXi’an 710072People’s Republic of China

Gradient magnetic heterointerfaces have injected infinite vitality in optimizing impedance matching,adjusting dielectric/magnetic resonance and promoting electromagnetic(EM)wave absorption,but still exist a significant challenging in regulating local phase ***,accordion-shaped Co/Co_(3)O_(4)@N-doped carbon nanosheets(Co/Co_(3)O_(4)@NC)with gradient magnetic heterointerfaces have been fabricated via the cooperative high-temperature carbonization and lowtemperature oxidation *** results indicate that the surface epitaxial growth of crystal Co_(3)O_(4) domains on local Co nanoparticles realizes the adjustment of magnetic-heteroatomic components,which are beneficial for optimizing impedance matching and interfacial ***,gradient magnetic heterointerfaces simultaneously realize magnetic coupling,and long-range magnetic ***,the synthesized Co/Co_(3)O_(4)@NC absorbents display the strong electromagnetic wave attenuation capability of−53.5 dB at a thickness of 3.0 mm with an effective absorption bandwidth of 5.36 GHz,both are superior to those of single magnetic domains embedded in carbon *** design concept provides us an inspiration in optimizing interfacial polarization,regulating magnetic coupling and promoting electromagnetic wave absorption.

关键词： Magnetic heterointerfaces Phase evolution Interfacial polarization Magnetic coupling Electromagnetic wave absorption

Multiple Tin Compounds Modified Carbon Fibers to Construct heterogeneous Interfaces for Corrosion Prevention and Electromagnetic Wave Absorption

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Nano-Micro Letters 2025年第1期17卷 507-527页

作者： Zhiqiang guo Di Lan Zirui Jia Zhenguo Gao Xuetao Shi Mukun he Hua guo Guanglei Wu Pengfei Yin College of Science Sichuan Agricultural UniversityYa’an 625014People’s Republic of China School of Materials Science and Engineering Hubei University of Automotive TechnologyShiyan 442002People’s Republic of China Institute of Materials for Energy and Environment State Key Laboratory of Bio‑Fibers and Eco‑TextilesCollege of Materials Science and EngineeringQingdao UniversityQingdao 266071People’s Republic of China Shaanxi Key Laboratory of Macromolecular Science and Technology School of Chemistry and Chemical EngineeringNorthwestern Polytechnical UniversityXi’anShaanxi 710072People’s Republic of China

Currently,the demand for electromagnetic wave(EMW)absorbing materials with specific functions and capable of withstanding harsh environments is becoming increasingly ***-component interface engineering is considered an effective means to achieve high-efficiency EMW ***,interface modulation engineering has not been fully discussed and has great potential in the field of EMW *** this study,multi-component tin compound fiber composites based on carbon fiber(CF)substrate were prepared by electrospinning,hydrothermal synthesis,and high-temperature thermal *** utilizing the different properties of different substances,rich heterogeneous interfaces are *** effectively promotes charge transfer and enhances interfacial polarization and conduction *** prepared SnS/SnS_(2)/SnO_(2)/CF composites with abundant heterogeneous interfaces have and exhibit excellent EMW absorption properties at a loading of 50 wt%in epoxy *** minimum reflection loss(RL)is−46.74 dB and the maximum effective absorption bandwidth is 5.28 ***,SnS/SnS_(2)/SnO_(2)/CF epoxy composite coatings exhibited long-term corrosion resistance on Q235 steel ***,this study provides an effective strategy for the design of high-efficiency EMW absorbing materials in complex and harsh environments.

关键词： Electrostatic spinning Component regulation heterogeneous interfaces Electromagnetic wave absorption Corrosion protection

Exosomes:the next-generation therapeutic platform for ischemic stroke

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Neural Regeneration Research 2025年第5期20卷 1221-1235页

作者： Wenjing Yin Hongyin Ma yang Qu Jiaxin Ren Yingying Sun Zhen-Ni guo Yi yang Stroke Center Department of NeurologyFirst Hospital of Jilin UniversityChangchunJilin ProvinceChina Neuroscience Research Center Department of NeurologyFirst Hospital of Jilin UniversityChangchunJilin ProvinceChina

Current therapeutic strategies for ischemic stroke fall short of the desired objective of neurological functional ***,there is an urgent need to develop new methods for the treatment of this *** are natural cell-derived vesicles that mediate signal transduction between cells under physiological and pathological *** have low immunogenicity,good stability,high delivery efficiency,and the ability to cross the blood–brain *** physiological properties of exosomes have the potential to lead to new breakthroughs in the treatment of ischemic *** rapid development of nanotechnology has advanced the application of engineered exosomes,which can effectively improve targeting ability,enhance therapeutic efficacy,and minimize the dosages *** in technology have also driven clinical translational research on *** this review,we describe the therapeutic effects of exosomes and their positive roles in current treatment strategies for ischemic stroke,including their antiinflammation,anti-apoptosis,autophagy-regulation,angiogenesis,neurogenesis,and glial scar formation reduction ***,it is worth noting that,despite their significant therapeutic potential,there remains a dearth of standardized characterization methods and efficient isolation techniques capable of producing highly purified *** optimization strategies should prioritize the exploration of suitable isolation techniques and the establishment of unified workflows to effectively harness exosomes for diagnostic or therapeutic applications in ischemic ***,our review aims to summarize our understanding of exosome-based treatment prospects in ischemic stroke and foster innovative ideas for the development of exosome-based therapies.

关键词： blood–brain barrier electroacupuncture engineering exercise exosomes ischemic stroke mesenchymal stem cells microglia neuroprotection stents

核因子-κB在HUVEC细胞凋亡信号通路中的作用

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中国应用生理学杂志 2009年第3期25卷 383-386,I0005页

作者：薛凤麟张旋贺国洋王殿华昆明医学院第一附属医院病理科云南昆明650032 昆明医学院云南省天然药物药理重点实验室云南昆明650031

目的:探讨核因子-κB(NF-κB)在人脐静脉内皮细胞凋亡信号通路中的作用。方法:体外培养人脐静脉内皮细胞系(HUVEC),实验分为正常对照组、AngII组和Gliotoxin干预组。应用改良MTT法,观察0.01μmol/L、0.1μmol/L、1μmol/L和10μmol/L4... 详细信息

目的:探讨核因子-κB(NF-κB)在人脐静脉内皮细胞凋亡信号通路中的作用。方法:体外培养人脐静脉内皮细胞系(HUVEC),实验分为正常对照组、AngII组和Gliotoxin干预组。应用改良MTT法,观察0.01μmol/L、0.1μmol/L、1μmol/L和10μmol/L4种浓度的AngII在不同时间对HUVEC细胞活性的影响。应用DNA凝胶电泳和流式细胞术检测AngII作用于细胞后引起细胞凋亡的情况。应用免疫细胞化学技术检测NF-κBp65的核移位,评价NF-κB活化情况。结果:10μmol/LAngII作用于细胞24h时,细胞活性下降,DNA凝胶电泳和流式细胞结果提示细胞发生凋亡,凋亡细胞率明显高于正常对照组,差异具有统计学意义(P<0.05),0.1mg/LGliotoxin可拮抗AngII的细胞抑制活性作用;免疫细胞化学技术显示,HUVEC细胞经AngII诱导后,NF-κB出现明显核移位现象,提示NF-κB发生活化;Gliotoxin明显抑制NF-κB活化,与AngII组相比,差异有统计学意义(P<0.05)。结论:①AngII可引起HU-VEC细胞发生凋亡;而NF-κB特异性抑制剂Gliotoxin能够拮抗AngII对HUVEC细胞的作用;②NF-κB可能是AngII调控HUVEC细胞生存/凋亡通路中的重要信号转导分子。

关键词：核因子-κB 血管紧张素Ⅱ 人脐静脉内皮细胞凋亡

Induced neural stem cells regulate microglial activation through Akt-mediated upregulation of CXCR4 and Crry in a mouse model of closed head injury

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Neural Regeneration Research 2025年第5期20卷 1416-1430页

作者： Mou Gao Qin Dong Dan Zou Zhijun yang Lili guo Ruxiang Xu Department of Neurosurgery Sichuan Academy of Medical Sciences and Sichuan Provincial People’s HospitalSchool of MedicineUniversity of Electronic Science and Technology of ChinaChengduSichuan ProvinceChina Department of Neurosurgery Chinese PLA General HospitalBeijingChina Department of Neurology Fu Xing HospitalCapital Medical UniversityBeijingChina

Microglial activation that occurs rapidly after closed head injury may play important and complex roles in neuroinflammation-associated neuronal damage and *** previously reported that induced neural stem cells can modulate the behavior of activated microglia via CXCL12/CXCR4 signaling,influencing their activation such that they can promote neurological ***,the mechanism of CXCR4 upregulation in induced neural stem cells remains *** this study,we found that nuclear factor-κB activation induced by closed head injury mouse serum in microglia promoted CXCL12 and tumor necrosis factor-αexpression but suppressed insulin-like growth factor-1 ***,recombinant complement receptor 2-conjugated Crry(CR2-Crry)reduced the effects of closed head injury mouse serum-induced nuclear factor-κB activation in microglia and the levels of activated microglia,CXCL12,and tumor necrosis factor-α.Additionally,we observed that,in response to stimulation(including stimulation by CXCL12 secreted by activated microglia),CXCR4 and Crry levels can be upregulated in induced neural stem cells via the interplay among CXCL12/CXCR4,Crry,and Akt signaling to modulate microglial *** agreement with these in vitro experimental results,we found that Akt activation enhanced the immunoregulatory effects of induced neural stem cell grafts on microglial activation,leading to the promotion of neurological recovery via insulin-like growth factor-1 secretion and the neuroprotective effects of induced neural stem cell grafts through CXCR4 and Crry upregulation in the injured cortices of closed head injury ***,these beneficial effects of Akt activation in induced neural stem cells were positively correlated with the therapeutic effects of induced neural stem cells on neuronal injury,cerebral edema,and neurological disorders post–closed head *** conclusion,our findings reveal that Akt activation may enhance the immunoregulatory effects of induced neura

关键词： Akt signaling cerebral edema closed head injury Crry CXCR4 induced neural stem cell microglia neuroinflammation

Effects of the extrusion parameters on microstructure,texture and room temperature mechanical properties of extruded Mg-2.49Nd-1.82Gd-0.2Zn-0.2Zr alloy

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 136-146页

作者： Chenjin Zhang Guangyu yang Lei Xiao Zhiyong Kan Jing guo Qiang Li Wanqi Jie State Key Laboratory of Solidification Processing Northwestern Polytechnical UniversityXi’an 710072China College of Materials Science and Engineering Fuzhou UniversityFuzhou 350108China College of Chemistry and Environmental Science Qujing Normal UniversityQujing 655011China

Microstructure,texture,and mechanical properties of the extruded Mg-2.49Nd-1.82Gd-0.2Zn-0.2Zr alloy were investigated at different extrusion temperatures(260 and 320℃),extrusion ratios(10:1,15:1,and 30:1),and extrusion speeds(3 and 6 mm/s).The experimental results exhibited that the grain sizes after extrusion were much finer than that of the homogenized alloy,and the second phase showed streamline distribution along the extrusion direction(ED).With extrusion temperature increased from 260 to 320℃,the microstructure,texture,and mechanical properties of alloys changed *** dynamic recrystallization(DRX)degree and grain sizes enhanced as the extrusion ratio increased from 10:1 to 30:1,and the strength gradually decreased but elongation(EL)*** the extrusion speed increased from 3 to 6 mm/s,the grain sizes and DRX degree increased significantly,and the samples presented the typical-rare-earth(RE)*** alloy extruded at 260℃ with extrusion ratio of 10:1 and extrusion speed of 3 mm/s showed the tensile yield strength(TYS)of 213 MPa and EL of 30.6%.After quantitatively analyzing the contribution of strengthening mechanisms,it was found that the grain boundary strengthening and dislocation strengthening played major roles among strengthening *** results provide some guidelines for enlarging the industrial application of extruded Mg-RE alloy.

关键词： Mg-rare earth alloys extrusion temperature extrusion ratio extrusion speed strengthening mechanisms