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Silencing Huwe1 reduces apoptosis of cortical neurons exposed to oxygen-glucose deprivation and reperfusion

Neural Regeneration Research 2019年第11期14卷 1977-1985页

作者： guo-qian He Wen-Ming Xu Hui-Juan Liao Chuan Jiang Chang-Qing Li Wei Zhang Department of Pediatrics Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University)Ministry of EducationWest China Second University HospitalSichuan UniversityChengduSichuan ProvinceChina Joint Laboratory of Reproductive Medicine Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University)Ministry of EducationWest China Second University HospitalSichuan UniversityChengduSichuan ProvinceChina Department of Neurology The Second Affiliated Hospital of Chongqing Medical UniversityChongqingChina Department of Medical Oncology Sichuan Cancer Hospital & InstituteSichuan Cancer CenterCancer Hospital Affiliated to School of MedicineUniversity of Electronic Science and Technology of ChinaChengduSichuan ProvinceChina

HECT, UBA and WWE domain-containing 1(Huwe1), an E3 ubiquitin ligase involved in the ubiquitin-proteasome system, is widely expressed in brain tissue. Huwe1 is involved in the turnover of numerous substrates, including p53, Mcl-1, Cdc6 and N-myc, thereby playing a critical role in apoptosis and neurogenesis. However, the role of Huwe1 in brain ischemia and reperfusion injury remains unclear. Therefore, in this study, we investigated the role of Huwe1 in an in vitro model of ischemia and reperfusion injury. At 3 days in vitro, primary cortical neurons were transduced with a control or shRNA-Huwe1 lentiviral vector to silence expression of Huwe1. At 7 days in vitro, the cells were exposed to oxygen-glucose deprivation for 3 hours and reperfusion for 24 hours. To examine the role of the c-Jun N-terminal kinase(JNK)/p38 pathway, cortical neurons were pretreated with a JNK inhibitor(SP600125) or a p38 MAPK inhibitor(SB203508) for 30 minutes at 7 days in vitro, followed by ischemia and reperfusion. Neuronal apoptosis was assessed by TUNEL assay. Protein expression levels of JNK and p38 MAPK and of apoptosis-related proteins(p53, Gadd45 a, cleaved caspase-3, Bax and Bcl-2) were measured by western blot assay. Immunofluorescence labeling for cleaved caspase-3 was performed. We observed a significant increase in neuronal apoptosis and Huwe1 expression after ischemia and reperfusion. Treatment with the shRNA-Huwe1 lentiviral vector markedly decreased Huwe1 levels, and significantly decreased the number of TUNEL-positive cells after ischemia and reperfusion. The silencing vector also downregulated the pro-apoptotic proteins Bax and cleaved caspase-3, and upregulated the anti-apoptotic proteins Gadd45 a and Bcl-2. Silencing Huwe1 also significantly reduced p-JNK levels and increased p-p38 levels. Our findings show that downregulating Huwe1 affects the JNK and p38 MAPK signaling pathways as well as the expression of apoptosis-related genes to provide neuroprotection during ische

关键词： nerve regeneration ischemic stroke oxygen-glucose deprivation and reperfusion ischemia/reperfusion cortical neuron ubiquitin proteasome system Huwe1 apoptosis therapeutic targets cell culture cell death neural regeneration

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Microwave ablation is as effective as radiofrequency ablation for very-early-stage hepatocellular carcinoma

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Chinese Journal of Cancer 2017年第5期36卷 231-240页

作者： Yun Xu qiang Shen Neng Wang Pan-Pan Wu Bin Huang Ming Kuang guo-Jun qian Department of Minimal Invasion Therapy Eastern Hepatobiliary Surgery Hospital The Second Military Medical University of Chinese PLA 225# Changhai Rd Shanghai 200438 P. R. China Department of Radiology Eastern Hepatobiliary Surgery Hospital The Second Military Medical University of Chinese PLA Shanghai 200438 P. R. China Division of Interventional Ultrasound Department Hepatobiliary Surgery The First Affiliated Hospital of Sun Yat-sen University 58# Zhongshan Road 2nd Guangzhou 510080 Guangdong P. R.China

Background: Percutaneous radiofrequency ablation(RFA) is a first?line treatment for very?early?stage hepatocellular carcinoma(HCC), whereas the efficacy of percutaneous microwave ablation(MWA) for very?early?stage HCC remains unclear. The purpose of this study was to clarify this issue by comparing the safety and efficacy of percutaneous MWA with percutaneous RFA in treating very?early?stage ***: Clinical data of 460 patients who were diagnosed with very?early?stage HCC and treated with percutane?ous MWA or RFA between January 2007 and July 2012 at the Eastern Hepatobiliary Surgery Hospital, The Second Mili?tary Medical University, in Shanghai, China were retrospectively analyzed. Of these 460 patients, 159 received RFA, 301 received MWA. Overall survival(OS), recurrence?free survival(RFS), local tumor progression(LTP), complete ablation, and complication occurrence rates were compared between the two groups, and the prognostic factors associated with survival were ***: No significant differences were observed between the two groups in terms of the 1?, 3?, or 5?year OS rates(99.3%, 90.4%, and 78.3% for MWA vs. 98.7%, 86.8%, and 73.3% for RFA, respectively; P = 0.331). Furthermore, no signif?icant differences were observed between the two groups in terms of the corresponding RFS rates(94.4%, 71.8%, and 46.9% for MWA vs. 89.9%, 67.3%, and 54.9% for RFA, respectively; P ete ablation rates(98.3% vs. 98.1%, P = 0.309), the LTP rates(9.6% vs. 10.1%, P = 0.883), the compl multivariate analysis, LTP, an= 0.860), or the occurrence rates of major complications(0.7% vs. 0.6%, P = 0.691). Bytiviral therapy, and treatment of recurrence were independent risk fac?tors for OS(P < 0.001), and the alpha?fetoprotein level was an independent prognostic factor for RFS(P = 0.002).Conclusions: MWA is as safe and effective as RFA in treating very?early?stage HCC, supporting MWA as a first?line treatment option for this disease.

关键词： Microwave ablation Radiofrequency ablation Hepatocellular carcinoma

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De-novo characterization of the soft-shelled turtle Pelodiscus sinensis transcriptome using Illumina RNA-Seq technology

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第1期14卷 58-67页

作者： Wei WANG Cai-yan LI Chu-tian GE Lei LEI You-ling GAO guo-ying qian Zhejiang Provincial Top Key Discipline of Modern Microbiology and Application College of Biological and Environmental Sciences Zhejiang Wanli University Ningbo 315100 China Henan Provincial Bureau of Animal Husbandry Zhengzhou 450008 China

The soft-shelled turtle Pelodiscus sinensis is a high-profile turtle species because of its nutritional and medicinal value in Asian countries. However, little is known about the genes that are involved in formation of their nutritional quality traits, especially the molecular mechanisms responsible for unsaturated fatty acid and collagen biosynthesis. In the present study, the transcriptomes from six tissues from Pelodiscus sinensis were sequenced using an IIlumina paired-end sequencing platform. We obtained more than 47 million sequencing reads and 73954 unigenes with an average size of 754 bp by de-novo assembly. In total, 55.19% of the unigenes （40 814） had significant similarity with proteins in the National Center of Biotechnology Information （NCBI） non-redundant protein database and Swiss-Prot database （E-value 〈10-5）. Of these annotated unigenes, 9156 and 11 947 unigenes were assigned to 52 gene ontology categories （GO） and 25 clusters of orthologous groups （COG）, respectively. In total, 26496 （35.83%） unigenes were assigned to 242 pathways using the Kyoto Encyclopedia of Genes and Genomes pathway database （KEGG）. In addition, we found a number of highly expressed genes involved in the regulation of P. sinensis unsatu- rated fatty acid biosynthesis and collagen formation, including desaturases, growth factors, transcription factors, and extracellular matrix components. Our data represent the most comprehensive sequence resource available for the Chinese soft-shelled turtle and could provide a basis for new research on this turtle as well as the molecular genetics and functional genomics of other terrapins. To our knowledge, we report for the first time, the large-scale RNA se- quencing （RNA-Seq） of terrapin animals and would enrich the knowledge of turtles for future research.

关键词： Pelodiscus sinensis Illumina RNA-Seq Transcriptome Gene expression

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Pathological complete response to neoadjuvant alectinib in unresectable anaplastic lymphoma kinase positive non-small cell lung cancer:A case report

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World Journal of Clinical Cases 2023年第22期11卷 5322-5328页

作者： Lu-Ming Wang Peng Zhao Xu-Qi Sun Feng Yan qian guo Department of Thoracic Surgery The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310002Zhejiang ProvinceChina Department of Medical Oncology The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310002Zhejiang ProvinceChina Department of Medical Oncology The First People’s Hospital of Hangzhou Lin’an DistrictHangzhou 311300Zhejiang ProvinceChina

BACKGROUND The development of anaplastic lymphoma kinase(ALK)-tyrosine kinase inhibitors(TKIs)has remarkably improved the prognosis of patients with ALK-positive advanced non-small cell lung cancer(NSCLC).Alectinib,the second-generation ALK-TKI,has been approved as first-line treatment for advanced or metastatic NSCLC patients with ALK *** therapy can achieve tumor downstaging and eradicate occult lesions in patients with potentially resectable *** neoadjuvant alectinib can be a conversion therapy in ALK-positive advanced NSCLC patients remains *** SUMMARY A 41-year-old man was pathologically diagnosed with locally advanced ALKpositive stage IIIB *** was prescribed to induce tumor downstaging and facilitate the subsequent surgical *** tumor was successfully downstaged and pathological complete response was *** upper lobectomy with mediastinal lymphadenectomy was performed after tumor *** patient has continued to receive alectinib as adjuvant therapy during postoperative follow-up with a recurrence-free survival of 29 mo as of writing this *** This case sheds light on the feasibility and safety of alectinib as a neoadjuvant treatment for stage IIIB NSCLC patients with ALK *** efficacy needs to be validated in prospective clinical trials.

关键词： Alectinib Anaplastic lymphoma kinase Non-small cell lung cancer Neoadjuvant therapy Case report

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System identification and parameter estimation in mathematical medicine： examples demonstrated for prostate cancer

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Frontiers of Electrical and Electronic Engineering in China 2016年第1期4卷 13-19页

作者： Yoshito Hirata Kai Morino Taiji Suzuki qian guo Hiroshi Fukuhara Kazuyuki Aihara Institute of Industrial Science The University of Tokyo Tokyo 153-8505 Japan Graduate School of Information Science and Technology The University of Tokyo Tokyo 113-8656 Japan Department of Mathematical and Computing Sciences Graduate School of Information Science and Engineering Tokyo Institute of Technology Tokyo 152-8552 Japan Department of Mathematics Shanghai Normal University Shanghai 200234 China Department of Urology Graduate School of Medicine The University of Tokyo Tokyo 113-8655 Japan

We review our studies on how to identify the most appropriate models of diseases, and how to determine their parameters in a quantitative manner given a short time series of biomarkers, using intermittent androgen deprivation therapy of prostate cancer as an example. Recently, it has become possible to estimate the specific parameters of individual patients within a reasonable time by employing the information concerning other previous patients as a prior. We discuss the importance of using multiple mathematical methods simultaneously to achieve a solid diagnosis and prognosis in the future practice of personalized medicine.

关键词： mathematical medicine dynamical model parameter estimation

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Modeling and Analysis for Practical CT Based on Transient Test and Parameter Identification

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CSEE Journal of Power and Energy Systems 2016年第4期2卷 51-57页

作者： Xianggen Yin Zhe Zhang Xuanwei Qi Gan Li Wenbin Cao qian guo State Key Laboratory of Advanced Electromagnetic Engineering and Technology Huazhong University of Science and TechnologyWuhan 430074China

In the transient process of power grid faults,the transferring distortion of current transformer(CT)can seriously affect relay protection *** these conditions,it is difficult to analyze the ferromagnetic characteristic of the magnetizing branch in the transient equivalent circuit of *** Jiles-Atherton hysteresis model(J-A model),which is widely used in digital simulations,can accurately describe the hysteresis and saturation process of the core characteristics;however,to acquire the parameters of the J-A model of current transformers in practical use is still a challenging *** this paper,physical tests based on a practical CT and parameter identification are presented to solve the *** basic hysteresis loops of P,PR,and TPY class of practical current transformers are obtained through physical ***,the J-A model parameters are identified using a hybrid genetic/simulated annealing algorithm,based on which transient simulation models of different class CTs are *** effectiveness of the proposed method is verified via dynamic physical simulation tests.A typical accident is analyzed based on these models.

关键词： Current transformer dynamic physical simulation tests J-A model parameter identification transient test

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Development of calcium coke for CaC2 production using calcium carbide slag and coking coal

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International Journal of Minerals,Metallurgy and Materials 2021年第1期28卷 76-87页

作者： Xu-zhong Gong Jun-qiang Zhang Zhi Wang Dong Wang Jun-hao Liu Xiao-dong Jing guo-yu qian Chuan Wang Key Laboratory of Green Process and Engineering National Engineering Laboratory for Hydrometallurgical Cleaner Production TechnologyInstitute of Process EngineeringChinese Academy of SciencesBeijing 100190China Chemical Engineering School University of Chinese Academy of SciencesBeijing 100049China Innovation Academy for Green Manufacture Chinese Academy of SciencesBeijing 100190China Department of Process Metallurgy Swerim ABLulea 97125Sweden

A type of calcium coke was developed for use in the oxy-thermal process of calcium carbide *** calcium coke was prepared by the co-pyrolysis of coking coal and calcium carbide slag, which is a solid waste generated from the chlor-alkali *** characteristics of the calcium cokes under different conditions were analyzed experimentally and *** results show that the thermal strength of calcium coke increased with the increase in the coking coal proportion, and the waterproof property of calcium coke also increased with increased carbonation *** calcium coke can increase the contact area of calcium and carbon in the calcium carbide production ***, the pore structure of the calcium coke can enhance the diffusion of gas inside the furnace, thus improving the efficiency of the oxy-thermal technology.

关键词： calcium carbide slag calcium carbide production calcium coke thermal strength waterproof property

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Small interfering RNA delivery to the neurons near the amyloid plaques for improved treatment of Alzheimer’s disease

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Acta Pharmaceutica Sinica B 2019年第3期9卷 590-603页

作者： qian guo Xiaoyao Zheng Peng Yang Xiaoying Pang Kang qian Pengzhen Wang Shuting Xu Dongyu Sheng Liuchang Wang Jinxu Cao Wei Lu Qizhi Zhang Xinguo Jiang Key Laboratory of Smart Drug Delivery Ministry of Education & State Key Laboratory of Molecular Engineering of Polymers School of Pharmacy Fudan University

Gene therapy represents a promising treatment for the Alzheimer’s disease(AD). However,gene delivery specific to brain lesions through systemic administration remains big challenge. In our previous work, we have developed an siRNA nanocomplex able to be specifically delivered to the amyloid plaques through surface modification with both CGN peptide for the blood–brain barrier(BBB)penetration and QSH peptide for β-amyloid binding. But, whether the as-designed nanocomplex could indeed improve the gene accumulation in the impaired neuron cells and ameliorate AD-associated symptoms remains further study. Herein, we prepared the nanocomplexes with an siRNA against β-site amyloid precursor protein-cleaving enzyme 1(BACE1), the rate-limiting enzyme of Aβ production, as the therapeutic siRNA of AD. The nanocomplexes exhibited high distribution in the Aβ deposits-enriched hippocampus, especially in the neurons near the amyloid plaques after intravenous administration. In APP/PS1 transgenic mice, the nanocomplexes down-regulated BACE1 in both mRNA and protein levels,as well as Aβ and amyloid plaques to the level of wild-type mice. Moreover, the nanocomplexes significantly increased the level of synaptophysin and rescued memory loss of the AD transgenic mice without hematological or histological toxicity. Taken together, this work presented direct evidences that the design of precise gene delivery to the AD lesions markedly improves the therapeutic outcome.

关键词： siRNA delivery Neurons Amyloid plaques BACE1 gene Alzheimer’s disease

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AST-to-ALT ratio and coronary artery lesions among patients with Kawasaki disease

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World Journal of Pediatrics 2021年第6期17卷 659-668页

作者： Lei Cao Yun-Jia Tang Miao Gang Jin Ma Wei-guo qian Qiu-Qin Xu Hai-Tao Lv Department of Cardiology Children's Hospital of Soochow UniversityNo.92Zhongnan StreetSuzhouChina Department of Pharmacy Children's Hospital of Soochow UniversityNo 92Zhongnan StreetSuzhou.China

Background The aim of this study was to explore the associations between the aspartate aminotransferase-to-alanine aminotransferase ratio(AST/ALT)and coronary artery lesions(CALs)among patients with Kawasaki disease(KD).Methods Medical records of KD patients presenting to a single center between January 2019 and December 2020 were retrospectively collected and ***,multivariable-adjusted analyses,subgroup analyses,restricted cubic spline test,and fitted curves were used to evaluate the associations between AST/ALT and *** A total of 831 patients were enrolled,of which 201(24.2%)had CALs on admission and 21(2.5%)developed CALs de novo after intravenous immunoglobulin(IVIG).Multivariable-adjusted analyses models revealed that a lower AST/ALT was associated with an increased risk of CALs on admission when AST/ALT was a continuous variable(P=0.007)and when it was a categorical variable(P for trend=0.004).Each unit increase in AST/ALT was associated with a 22%lower risk of CALs on admission(odds ratio=0.78,95%confidence interval 0.65-0.94).A negative linear relationship was noted between AST/ALT and the risk of CALs on admission in both observed and fitted ***,such associations were not observed in AST/ALT and CALs de novo after *** of the variables significantly modified the association between AST/ALT and CALs on admission and CALs de novo after IVIG(P>0.05).Conclusion Our findings suggested that AST/ALT was a risk factor of CALs,but was not associated with progressive CALs.

关键词： Alanine aminotransferase Aspartate aminotransferase Coronary artery lesions Kawasaki disease

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A REVIEW OF INTEGRATED EXPLORATION METHOD OF OIL AND GAS

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石油勘探与开发 1995年第2期 90-96页

作者： Deng Long-wu guo qian-jie(Exploration Bureau of China Naional Petroleum Corporation)

ＡＲＥＶＩＥＷＯＦＩＮＴＥＧＲＡＴＥＤＥＸＰＬＯＲＡＴＩＯＮＭＥＴＨＯＤＯＦＯＩＬＡＮＤＧＡＳ￥ＤｅｎｇＬｏｎｇ－ｗｕ；ＧｕｏＱｉａｎ－ｊｉｅ（ＥｘｐｌｏｒａｔｉｏｎＢｕｒｅａｕｏｆＣｈｉｎａＮａｉｏｎａｌＰｅｔｒｏ... 详细信息

ＡＲＥＶＩＥＷＯＦＩＮＴＥＧＲＡＴＥＤＥＸＰＬＯＲＡＴＩＯＮＭＥＴＨＯＤＯＦＯＩＬＡＮＤＧＡＳ￥ＤｅｎｇＬｏｎｇ－ｗｕ；ＧｕｏＱｉａｎ－ｊｉｅ（ＥｘｐｌｏｒａｔｉｏｎＢｕｒｅａｕｏｆＣｈｉｎａＮａｉｏｎａｌＰｅｔｒｏｌｅｕｍＣｏｒｐｏｒａｔｉｏｎ）Ｔ...

关键词： A REVIEW OF INTEGRATED EXPLORATION METHOD OF OIL AND GAS

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