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Horticulture Research 2021年第1期8卷 2861-2871页

作者： guizhi feng Xiu Ai Hualin Yi Wenwu Guo Juxun Wu Key Laboratory of Horticultural Plant Biology Ministry of EducationHuazhong Agricultural UniversityWuhanPR China

Valencia orange(Citrus sinensis Osbeck)(VO)is a type of late-ripening sweet orange whose ripening occurs 4 to 5 months later than that of the mid-ripening common sweet orange(CO).Notably,the mastication trait of VO fruit is inferior to that of CO *** date,how inferior pulp mastication trait forms in VO has not been *** this study,13 VO varieties and 12 CO varieties were subjected to whole-genome resequencing.A total of 2.98 million SNPs were identified from 25 varieties,and a SNP molecular marker was developed to distinguish VO and ***,144 and 141 genes identified by selective sweep analysis were selected during VO and CO evolution,*** on gene functional enrichment analysis,most of the selected VO genes were related to the stress response and lignin ***,we comparatively analyzed the transcriptome profiles of peel and pulp tissues among three VO varieties and three CO varieties,and the results demonstrated differences in lignin biosynthesis between VO and CO ***,coexpression network analysis was performed to identify hub genes of lignin-related and variety-specific networks,which included CsERF74,CsNAC25,CsHSFB3,CsSPL4/13,***,this study provides important insights into the mastication trait formation of Valencia orange fruit.

关键词： orange insight varieties

Genome-wide comprehensive analysis of transcriptomes and small RNAs offers insights into the molecular mechanism of alkaline stress tolerance in a citrus rootstock

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Horticulture Research 2019年第1期6卷 1315-1333页

作者： Juxun Wu Junying Cao Mei Su guizhi feng Yanhui Xu Hualin Yi Key Laboratory of Horticultural Plant Biology Ministry of EducationHuazhong Agricultural UniversityWuhan 430070PR China

Alkaline stress has serious-negative effects on citrus *** xiangcheng(Citrus junos *** Tanaka)(Cj)is a rootstock that is tolerant to alkaline stress and iron *** orange(Poncirus trifoliata(L.)Raf.)(Pt),the most widely used rootstock in China,is sensitive to alkaline *** investigate the molecular mechanism underlying the tolerance of Cj to alkaline stress,next-generation sequencing was employed to profile the root transcriptomes and small RNAs of Cj and Pt seedlings that were cultured in nutrient solutions along a three pH *** two-level regulation data set provides a system-level view of molecular events with a precise *** data suggest that the auxin pathway may play a central role in the inhibitory effect of alkaline stress on root growth and that the regulation of auxin homeostasis under alkaline stress is important for the adaptation of citrus to alkaline ***,the jasmonate(JA)pathway exhibits the opposite response to alkaline stress in Cj and Pt and may contribute to the differences in the alkaline stress tolerance and iron acquisition between Cj and *** dataset provides a wealth of genomic resources and new clues to further study the mechanisms underlying alkaline stress resistance in Cj.

关键词： alkaline stress citrus

Vitapex在难治性慢性根尖周炎治疗中的疗效观察

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医学研究杂志 2009年第9期38卷 107-108页

作者：冯桂芝山东省聊城职业技术学院 252000

目的观察慢性根尖周炎治疗中使用Vitapex糊剂进行长期根管封药的疗效。方法用Vitapex糊剂对70颗患牙进行长期封药观察,术后1年随访临床疗效。结果70颗患牙有68颗获得了成功,成功率达97%。结论Vitapex是一种较好的根管充填糊剂,可缓解组... 详细信息

目的观察慢性根尖周炎治疗中使用Vitapex糊剂进行长期根管封药的疗效。方法用Vitapex糊剂对70颗患牙进行长期封药观察,术后1年随访临床疗效。结果70颗患牙有68颗获得了成功,成功率达97%。结论Vitapex是一种较好的根管充填糊剂,可缓解组织的炎性反应,促进根尖周组织炎症愈合,值得推广应用。

关键词： Vitapex糊剂根管治疗慢性根尖周炎

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不同剂量米非司酮治疗子宫肌瘤的疗效观察

中国社区医师 2020年第33期36卷 27-28页

作者：冯桂芝云南省楚雄州武定县人民医院妇产科云南楚雄651600

目的:分析不同剂量米非司酮治疗子宫肌瘤的疗效。方法:2019年1月-2020年1月收治子宫肌瘤患者82例,随机分为两组,各41例。对照组采用常规剂量米非司酮治疗;试验组采用低剂量米非司酮治疗。比较两组卵巢功能、临床疗效及不良反应发生情况... 详细信息

目的:分析不同剂量米非司酮治疗子宫肌瘤的疗效。方法:2019年1月-2020年1月收治子宫肌瘤患者82例,随机分为两组,各41例。对照组采用常规剂量米非司酮治疗;试验组采用低剂量米非司酮治疗。比较两组卵巢功能、临床疗效及不良反应发生情况。结果:试验组雌激素(E2)、卵泡刺激素(FSH)、黄体生成素(LH)水平均显著优于对照组,差异有统计学意义(P<0.05);试验组症状改善率高于对照组,差异有统计学意义(P<0.05);试验组不良反应发生率低于对照组,差异有统计学意义(P<0.05)。结论:小剂量米非司酮治疗子宫肌瘤,可改善患者的雌孕激素水平,药物治疗安全、有效。

关键词：不同剂量米非司酮子宫肌瘤

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季节性河床不同沙样的水分特征曲线研究

人民黄河 2017年第1期39卷 57-61页

作者：武桂芝李宁宁冯增帅杨帆青岛理工大学环境与市政工程学院山东青岛266033

选取季节性河流大沽河上、中、下游3处代表性河床的沙样作为试验样品,采用张力计法测出不同沙样的脱湿和吸湿过程,采用Van Genuchten模型拟合不同沙样的水分特征曲线,利用MATLAB中的Nlinfit函数求得Van Genuchten模型参数。结果表明:土... 详细信息

选取季节性河流大沽河上、中、下游3处代表性河床的沙样作为试验样品,采用张力计法测出不同沙样的脱湿和吸湿过程,采用Van Genuchten模型拟合不同沙样的水分特征曲线,利用MATLAB中的Nlinfit函数求得Van Genuchten模型参数。结果表明:土壤水吸力(水银柱高度)为0~10 cm时,沙样的质量含水率变化明显,随着土壤水吸力逐渐增大,质量含水率逐渐降低,当土壤水吸力为60 cm左右时,含水率变化减缓,逐渐趋于平稳;3种沙样均存在水分特征曲线滞后现象,粗沙的滞后现象较细沙的明显;实测水分特征曲线和拟合曲线基本吻合,Van Genuchten模型和MATLAB求参方法均适用于拟合季节性河床沙样的水分特征曲线。

关键词：水分特征曲线 MATLAB Van Genuchten模型大沽河

产教融合背景下高职医药类专业“双师型”教师队伍建设研究

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卫生职业教育 2024年第3期42卷 4-7页

作者：訾丽丽陈国华冯桂芝聊城职业技术学院山东聊城252000

“双师型”教师队伍是我国职业教育高质量发展的重要支撑。通过分析高职院校医药类专业“双师型”教师队伍建设中存在的问题,阐释产教融合背景下“双师型”教师队伍建设的优势,提出高职院校医药类专业“双师型”教师队伍建设路径。

关键词：产教融合高职医药类专业 “双师型”教师

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浅谈口腔工艺技术教学改革的迫切性及策略

卫生职业教育 2009年第5期27卷 16-17页

作者：谢丽娜李鹏冯贵芝聊城职业技术学院

随着现代口腔医学的进步，我国口腔修复学也得到快速发展。但口腔工艺学的教学现状却不容乐观，教学中存在许多问题，为改变这种现状，大力推进我国口腔工艺学高等教学改革势在必行。

关键词：口腔工艺技术教学改革

Ge Film Contacts to Radio Frequency Sputtered Y-Ba-Cu-O Superconductor Film

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Chinese Physics Letters 1991年第9期8卷 487-490页

作者： ZHANG Bei QIAN Yi TONG Litai CHEN Haiying WANG guizhi QIAO Yongping XING Miaoxiang feng Qingrong LIU Zunxiao LAN Jian DAI Yuandong Department of Physics Peking UniversityBeijing 100871

The semiconductor-superconductor con tact system of Ge/Y-Ba-Cu-O(YBCO)/SrTiO_(3) has been successfully fabricated by two steps of *** YBCO film in the contact of Ge/YBCO did not lose its superconductivity and its T_(c)(on set)did not degrade as *** top layer of Ge was polycrystalline with preferred orientation corresponding to the epitaxial structure of the underlayer of YBCO.

关键词： deposition steps superconductor

Identification of differentially expressed proteins in human stage Ⅰ lung adenocarcinoma and tumor-adjacent tissues with two-dimension gel electrophoresis profiling

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The Chinese-German Journal of Clinical Oncology 2009年第7期8卷 388-392页

作者： Feifei feng guizhi Liu Yiming Wu Department of Occupational Health College of Public Health of Zhengzhou University Zhengzhou 450001 China Department of Physical Examination First Teaching Hospital Zhengzhou University Zhengzhou 450052 China

Objective： The aim of this study was to establish reproducible two-dimensional electrophoretic assay used for profiling and identification of differentially expressed proteins in human stage I lung adenocarcinoma and paired normal tumor-adjacent tissue. Methods： The proteins from 12 human stage I lung adenocarcinoma tissues and normal tumor-adjacent tissues were separated using isoelectric focusing electrophoresis （the first dimension） and the subsequent homogeneous SDS-polyacrylamide gel electrophoresis （SDS-PAGE）（the second dimension）. The differentially expressed proteins were determined with PDQuest image analysis software, and identified using matrix-assisted laser desorption/ionization time of flight mass spectrometry （MALDI-TOF-MS） and database searching. Results： The well-reproducible 2-DE gel patterns of human stage I lung adenocarcinoma and normal tumor-adjacent tissues were profiled and 26 differentially expressed proteins uncovered. Nine of these 26 protein spots were cut out from the preparation gels and determined with MALDI-TOF-MS. Searching against the protein database, four candidate proteins were identified. They were 60S acidic ribosomal protein P2, Cathepsin B1, Apolipoprotein A-I precursor, and La 4.1 protein. Conclusion： In this study, high reproducible 2-DE gel protein images of human stage I lung adenocarcinoma and paired normal tumor-adjacent tissues were achieved successfully, and 4 differentially expressed proteins were revealed. These data will be helpful for screen of early biomarker and study of molecular mechanisms of human lung adenocarcinoma.

关键词： lung adenocarcinoma proteomics two-dimensional gel electrophoresis matrix-assisted laser desorption-ionization time of flight mass spectrometry (MALDI-TOF-MS)

Sleep disorders and its related risk factors in patients undergoing chronic peritoneal dialysis

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Chinese Medical Journal 2014年第7期127卷 1289-1293页

作者： Li Han Li Xiaobei feng Sujuan Zhang guizhi Wang Wei Wang Shixiang Department of Blood Purification Beijing Chao-Yang Hospital Capital Medical University Nephrology Faculty Capital Medical University Beijing 100020 China Institute of Urology Capital Medical University Beijing 100020China Department of Urology Beijing Chao-Yang Hospital Capital Medical University Beijing 100020 China

Background The prevalence of sleep disorders has been shown to be high in patients with chronic dialysis patients and may contribute to impaired quality of life and higher mortality in this ***,there are few data on the relationship of sleep disorders and their risk factors in chronic dialysis *** aim of this study was to evaluate the relationship of sleep disorders and their risk factors in chronic dialysis *** A total of 42 continuous ambulatory peritoneal dialysis （CAPD） patients were involved in this cross-sectional *** quality was assessed by the Pittsburgh Sleep Quality Index （PSQI）.Restless legs syndrome （RLS） was diagnosed according to the criteria of the International Restless Legs Syndrome Study *** depression was assessed by Hamilton depression *** information and laboratory data were *** The prevalence of sleep disorders was 47.6％ in the CAPD *** to the PSQI,the 42 CAPD patients were divided into sleep disturbance group and non-sleep disorders *** were no significant differences in age,gender,dialysis duration,hemoglobin,serum creatinine,urea nitrogen,β2-microglobulin,parathyroid hormone,calcium,and phosphorus between CAPD patients with sleep disorders and those without sleep *** the level of serum albumin （AIb） in CAPD patients with sleep disorders was significantly lower than that in CAPD patients without sleep disorders （31.3±1.4 vs.34.3±3.7,t=3.603,P=0.001）.And the prevalence of RLS and depression was significantly higher than that in CAPD patients without sleep disorders （RLS：11/22 vs.1/20,x2=10.395,P=0.001; depression：7/22 vs.1/20,x2=4.886,P=0.027）.In CAPD patients with RLS,the prevalence of sleep disorders was significantly higher than that in CAPD patients without RLS （11/22 vs.11/30,x2=10.395,P=0.001）.And in CAPD patients with depression,the prevalence of sleep disorders was significantly higher than that in CAPD patients without depressio

关键词： peritoneal dialysis sleep disorders depression restless legs syndrome serum albumin