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休闲视域下生态化高校体育课程体系改革研究

当代体育科技 2024年第6期14卷 42-45页

作者：申桂芳山西管理职业学院山西临汾041051

近年来,人们为了能够改善体质健康,预防社会文明病,开发出了一系列新兴概念,生态体育便是其中之一。生态体育的主要目的是探索人、体育、自然之间和谐共生的基本关系。将生态体育的理念融入高校体育教学实践中,促进生态化高校体育课程... 详细信息

近年来,人们为了能够改善体质健康,预防社会文明病,开发出了一系列新兴概念,生态体育便是其中之一。生态体育的主要目的是探索人、体育、自然之间和谐共生的基本关系。将生态体育的理念融入高校体育教学实践中,促进生态化高校体育课程体系的改革,不仅有利于更新现代体育教育理念,同时也可以促进当代青年学生的身体健康和心理健康,甚至使青年学生养成良好的思想道德品质。在具体的实践中,需要高校体育教师多措并举,逐渐完善高校体育教学的生态系统,建设高校体育生态化发展所需要的自然环境和文化环境等,使当代青年学生一边接受良好的体育教学,一边实现生态化的锻炼目标,从而全面提高生态化高校体育课程体系改革的效率。该文主要基于休闲视域,探讨生态化高校体育课程体系改革的基本内容。

关键词：休闲视域生态化高校体育课程体系改革研究

Establishment of Chlamydomonas reinhardtii chloroplast expression

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Chinese Science Bulletin 1999年第18期44卷 1659-1664页

作者： Guochang Fan Ning Su Zhonglin Zhang Xiangfu Wu guifang shen Chinese Acad Agr Sci Biotech Res Ctr Beijing 100081 Peoples R China Beijing Inst Radiat Med Beijing 100850 Peoples R China Chinese Acad Sci Shanghai Inst Biochem Shanghai 200031 Peoples R China

An expression vector pACⅢ containing the chimeric gene HAV- VP3P1 and HCV-C gene has been constructed and transferred to C. reinhardtii by the biolistic method. The trans-formants have been identified by PCR, Southern-blotting, Northern-blotting and Western-blotting assays after selecting on resistant medium and incubating in the dark. The results

关键词： HAV-VP3P1 gene HCV-C gene C. reinhardtii chloroplast expression system homologous recombinant.

JrATHB-12 mediates JrMYB113 and JrMYB27 to control the anthocyanin levels in different types of red walnut

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Journal of Integrative Agriculture 2024年第8期23卷 2649-2661页

作者： Haifeng Xu guifang Wang Xinying Ji Kun Xiang Tao Wang Meiyong Zhang Guangning shen Rui Zhang Junpei Zhang Xin Chen Key Laboratory of Huanghuai Protected Horticulture Engineering/National Germplasm Repository of Walnut and Chestnut Shandong Institute of PomologyTai’an 271000China Research Institute of Forestry Chinese Academy of ForestryBeijing 100091China College of Horticulture and Forestry Tarim UniversityAlaer 843300China

Red walnut has broad market prospects because it is richer in anthocyanins than ordinary ***,the mechanism driving anthocyanin biosynthesis in red walnut is still *** studied two types of red walnut,called red walnut 1(R1),with a red pericarp and seed coat,and red walnut 2(R2),with a red seed coat only.R1 mostly contained cyanidin-3-O-galactoside,while R2 contained a various amounts of cyanidin-3-Ogalactoside,cyanidin-3-O-arabinoside,and *** LDOX-2(LOC109007163)and LDOX-3(LOC109010746)genes,which encode leucoanthocyanidin dioxygenase/anthocyanidin synthase(LDOX/ANS),were preliminarily indicated as the crucial genes for anthocyanin biosynthesis in R1 and R2,*** MYB differential genes analysis showed that MYB27 and MYB113 are specifically expressed in the red parts of R1 and R2,respectively,and they are regarded as candidate regulatory *** expression in Arabidopsis and transient injection in walnut showed that both MYB27 and MYB113 were located in the nucleus and promoted anthocyanin accumulation,while MYB27 promoted the expression of LDOX-2,and MYB113 promoted the expression of LDOX-3and *** one-hybrid and electrophoretic mobility shift assays showed that MYB27 could only bind to the LDOX-2 promoter,while MYB113 could bind to the promoters of both LDOX-3 and *** addition,we also identified an HD-Zip transcription factor,ATHB-12,which is specifically expressed in the *** silencing the expression of ATHB-12,the R2 pericarp turned red,and MYB113 expression *** experiments showed that ATHB-12 could specifically interact with MYB113 and bind to its *** suggests that MYB27controls R1 coloration by regulating LDOX-2,while MYB113 controls R2 coloration by regulating LDOX-3 and UAGT-3,but ATHB-12 can specifically bind to and inhibit the MYB113 of the R2 pericarp so that it becomes *** study reveals the anthocyanin biosynthetic mechanisms in two differ

关键词： JrATHB-12 JrMYB113 JrMYB27 red walnut anthocyanin biosynthesis

Expression of human soluble TRAIL in Chlamydomonas reinhardtii chloroplast

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Chinese Science Bulletin 2006年第14期51卷 1703-1709页

作者： YANG Zongqi LI yinü CHEN Feng LI Dong ZHANG Zhifang LIU Yanxin ZHENG Dexian WANG Yong shen guifang College of Life Sciences Nankai University Tianjin 300071 China Biotechnology Research Institute Chinese Academy of AgriculturalSciences Beijing 100081 China National Laboratory of Medical Molecular Biology Institute of BasicMedical Sciences Chinese Academy of Medical Sciences and PekingUnion Medical College Beijing 100005 China

Tumor necrosis factor-related apop-tosis-inducing ligand (TRAIL) induces selectively apoptosis in various tumor cells and virus-infected cells, but rarely in normal cells. A chloroplast expres-sion vector, p64TRAIL, containing the cDNA coding for the soluble TRAIL (sTRAIL), was constructed with clpP-trnL-petB-chlL-rpl23-rpl2 as Chlamydomonas reinhardtii plastid homologous recombinant frag-ments and spectinomycin-resistant aadA gene as a select marker. The plasmid p64TRAIL was trans-ferred into the chloroplast genome of C. reinhardtii by the biolistic method. Three independently trans-formed lines were obtained by 100 mg/L spectino-mycin selection. PCR amplification, Southern blot analysis of the sTRAIL coding region DNA and culti-vation cells in the dark all showed that the exogenous DNA had been integrated into chloroplast genome of C. reinhardtii. Western blot analysis showed that human soluble TRAIL was expressed in C. reinhardtii chloroplast. The densitometric analysis of Western blot indicated that the expressed human sTRAIL protein in the chloroplasts of C. reinhardtii accounted for about 0.43%―0.67% of the total soluble proteins. These experimental results demonstrated the possi-bility of using transgenic chloroplasts of green alga as bioreactors for production of biopharmaceuticals.

关键词： TRAIL 叶绿体同源重组体衣藻

Changes in erythrocyte membrane ATPase and plasma lipid peroxides during perioperative period

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医学研究生学报 1993年第S1期6卷 50-54页

作者： Tu Weifeng Lin guifang shen Jianfan Department of Anesthesiology,Jinling Hospital,Nanjing,China Department of Anesthesiology,First Affiliated Hospital of Nanjing Medical College,Nanjing,China

Changes in erythrocyte membrane ATPases and plasma lipid peroxides（LPO） were studiedperioperatively in 15 abdominal surgical patients under intravenous procaine-balanced *** resultsrevealed that erythrocyte membrne Na+-K+ATPase,Mg2+ATPase and Ca2+-Mg2+ATPase activities werenot significantly changed at 60～90 min after incision as compared with 30 min before anesthesia,but weredecreased markedly at 10 rain and 24 hours after completion of operation（P0.05）.These correlations are suggested to be inducedby neuroendoerine responses after anesthesia and/or surgical trauma.

关键词： erythrocytes membrane ATPases lipid peroxides balanced anesthesia

应用德尔菲法建立高校体育社团的组织效能评价体系

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商丘职业技术学院学报 2015年第5期14卷 129-131页

作者：申桂芳山西管理职业学院山西临汾041051

运用德尔菲法对高校体育社团的组织效能评价指标进行了两轮专家调查,并采用数理统计法对调查结果进行归纳处理,最终确定了30个评价指标,其中社团在校内的知名度、社团领导的管理能力、是否注重培养重要成员3个指标平均得分排在前3.

关键词：高校学生体育社团组织效能评价组织效能指标

医护小组联合查房在规避ICU患者护理缺陷中的应用

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中西医结合护理（中英文） 2016年第1期2卷 45-47,50页

作者：申桂芳江苏省沛县人民医院江苏徐州221600

目的探讨医护小组联合查房在规避ICU患者护理缺陷中的应用效果。方法 ICU科室自2014年8月起改革查房模式,实施医护小组联合查房,由值班医生、责任护士搭配对患者进行查房。比较医护小组联合查房前后责任护士对患者病情掌握情况、患者护... 详细信息

目的探讨医护小组联合查房在规避ICU患者护理缺陷中的应用效果。方法 ICU科室自2014年8月起改革查房模式,实施医护小组联合查房,由值班医生、责任护士搭配对患者进行查房。比较医护小组联合查房前后责任护士对患者病情掌握情况、患者护理缺陷以及家属护理满意度的变化。结果医护小组联合查房后,责任护士疾病诊断、诊疗方案、护理落实等方面的掌握率显著高于联合查房前(P<0.05),ICU患者的压疮、意外拔管、VAP等发生率低于联合查房前(P<0.05),患者家属在风险防范、护理操作、护理结果方面的满意度高于联合查房前(P<0.05)。结论医护小组联合查房能够提高责任护士对ICU患者病情的掌握程度,规避护理缺陷,改善家属的护理满意度。

关键词：医护小组联合查房 ICU患者护理缺陷

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动、静脉给药治疗糖尿病足

中国现代医生 2009年第33期47卷 109-110页

作者：申桂芳江苏省沛县人民医院江苏沛县221600

目的探讨输液泵股动脉内给药治疗糖尿病足的疗效及安全性。方法将84例糖尿病足患者随机分为实验组43例及对照组41例;实验组经患侧股动脉给药,对照组全身静脉给药;两组均以葛根素500mg及甲钴胺500μg用生理盐水稀释后给药。治疗4周,治疗... 详细信息

目的探讨输液泵股动脉内给药治疗糖尿病足的疗效及安全性。方法将84例糖尿病足患者随机分为实验组43例及对照组41例;实验组经患侧股动脉给药,对照组全身静脉给药;两组均以葛根素500mg及甲钴胺500μg用生理盐水稀释后给药。治疗4周,治疗始末进行足部病变Vagner分级并比较治愈、好转、无效的例数,用轶和检验进行统计学分析。结果实验组治愈率(72.09%)明显高于对照组(36.59%),P<0.005;无效率(2.33%)明显低于对照组(26.93%),P=0.0009。两组不良反应差异无统计学意义。结论股动脉内动脉留置针给药是可行的,该方法疗效明显,安全性高。

关键词：糖尿病足股动脉输液泵

亚临床甲状腺功能减退对体检人群血清胃功能表达水平的影响

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医学研究杂志 2022年第12期51卷 89-91,95页

作者：王艳红庞训雷沈桂芳魏伟费素娟徐州医科大学附属医院消化内科 221004 徐州医科大学附属医院健康管理中心 221004

目的研究亚临床甲状腺功能减退(subclinical hypothyroidism,SCH)是否对体检人群血清胃功能表达水平产生影响。方法按照年龄、性别匹配原则收集2020年1月~2021年6月笔者医院健康管理中心确诊的幽门螺杆菌(Helicobacter pylori,Hp)阴性的... 详细信息

目的研究亚临床甲状腺功能减退(subclinical hypothyroidism,SCH)是否对体检人群血清胃功能表达水平产生影响。方法按照年龄、性别匹配原则收集2020年1月~2021年6月笔者医院健康管理中心确诊的幽门螺杆菌(Helicobacter pylori,Hp)阴性的SCH患者(SCH组)268例,同时选取Hp阴性的健康对照者(HC组)268例作为对照组。所有研究对象采集空腹静脉血测定血清促甲状腺释放激素(thyroid stimulating hormone,TSH)、FT_(4)、FT_(3)以及血清胃蛋白酶原(pepsinogen,PG)Ⅰ、PGⅡ、胃泌素17(gastrin 17,G17)的水平。结果与HC组比较,SCH组FT3更低(5.07±0.68pmol/L vs 4.92±0.73pmol/L)。另外,SCH组的PGⅠ表达水平更高[99.23(79.71,124.04)μg/L vs 112.70(89.83,142.20)μg/L]并且G17表达水平更高[1.44(0.90,4.97)pmol/L vs 1.94(1.02,4.97)pmol/L]。SCH组体检者中,体检者TSH表达水平越高,血清G17表达水平越高[1.54(0.94,3.36)pmol/L vs 2.32(1.17,6.81)pmol/L]。相关性分析显示,全部研究对象的血清G17水平与TSH呈正相关;与FT_(4)、FT_(3)呈负相关。多重线性回归结果表明,全部研究对象的血清G17水平与TSH呈正相关,与FT_(3)及FT_(4)呈负相关。结论血清G17与TSH水平呈正相关,高TSH可能通过提高G17水平而影响了胃黏膜分泌功能的调节。

关键词：胃蛋白酶原胃泌素亚临床甲状腺功能减退体检人群