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M6PR interacts with the HA2 subunit of influenza A virus to facilitate the fusion of viral and endosomal membranes

Science China(Life Sciences) 2024年第3期67卷 579-595页

作者： Yuzhen Hu Li Jiang guangwen wang Yangming Song Zhibo Shan Xuyuan wang Guohua Deng Jianzhong Shi Guobin Tian Xianying Zeng Liling Liu Hualan Chen Chengjun Li State Key Laboratory for Animal Disease Control and Prevention Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China

Influenza A virus(IAV) commandeers numerous host cellular factors for successful replication. However, very few host factors have been revealed to be involved in the fusion of viral envelope and late endosomal membranes. In this study, we identified cation-dependent mannose-6-phosphate receptor(M6PR) as a crucial host factor for the replication of IAV. We found that siRNA knockdown of M6PR expression significantly reduced the growth titers of different subtypes of IAV, and that the inhibitory effect of M6PR siRNA treatment on IAV growth was overcome by the complement of exogenously expressed M6PR. When A549 cells were treated with siRNA targeting M6PR,the nuclear accumulation of viral nucleoprotein(NP) was dramatically inhibited at early timepoints post-infection, indicating that M6PR engages in the early stage of the IAV replication cycle. By investigating the role of M6PR in the individual entry and post-entry steps of IAV replication, we found that the downregulation of M6PR expression had no effect on attachment, internalization, early endosome trafficking,or late endosome acidification. However, we found that M6PR expression was critical for the fusion of viral envelope and late endosomal membranes. Of note, M6PR interacted with the hemagglutinin(HA) protein of IAV, and further studies showed that the lumenal domain of M6PR and the ectodomain of HA2 mediated the interaction and directly promoted the fusion of the viral and late endosomal membranes,thereby facilitating IAV replication. Together, our findings highlight the importance of the M6PR–HA interaction in the fusion of viral and late endosomal membranes during IAV replication.

关键词： influenza A virus M6PR HA membrane fusion late endosome

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Deep structure of the Kumkol basin in the northern Tibetan Plateau and its resource environmental implications

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Science China Earth Sciences 2024年第11期67卷 3421-3435页

作者： Wenhui LI Zhanwu LU Rui GAO Xiaofan DENG Jingyuan LI guangwen wang Zhanxuan SUN Sinoprobe Laboratory of Ministry of Natural Resources Institute of Geology Chinese Academy of Geological Sciences School of Earth Sciences and Engineering Sun Yat-sen University South Branch of Sinopec Petroleum Engineering Geophysics Co. Ltd

The Kumkol basin is located in the northern Tibetan Plateau and is a closed plateau basin with an average altitude of>4000 m and an area of nearly 20000 km2. Its boundaries are limited by the Altyn Tagh fault, East Kunlun orogen and Qimantag orogen. Studying the deep structure of the Kumkol basin reveals 2 significant implications:（1） the basin has developed a large thickness of >7000 m Cenozoic continental sediments, recording the uplift history of the northern Tibetan Plateau, and（2）preliminary work indicates that the basin is likely to have oil and gas prospects. However, owing to the adverse natural conditions of the area and the strong tectonic activity in the Cenozoic, the latter of which was not conducive to hydrocarbon preservation,only regional geological mapping and petroleum exploration route surveys have been carried out, and there is no consensus on strata, structure and tectonic evolution. From 2021 to 2022, a deep seismic reflection profile implemented by the Second Tibetan Plateau Scientific Expedition and Research（STEP） project was the first high-resolution geophysical survey across the Kumkol basin. This study uses seismic reflection migration profiles, first-arrival wave tomographic imaging and previous research results to analyze the deep structure of the basin. The final merged model contains many features of tectonic and resource significance:（1） The Kumkol basin is ～90 km wide from north to south, with a basement depth of >9000 m. The main component is the Cenozoic continental deposits, which are divided into two major parts: the southern composite basin and the northern faulted basin. Owing to the later compression, the southern composite basin experienced significant deformation, but most parts still preserved their original sedimentary formations.（2） The structural deformation characteristics of the basin reveal a two-stage tectonic evolution process of the northern Tibetan Plateau in the Cenozoic: from the Oligocene to the Pliocene, the ma

关键词： Kumkol basin Seismic reflection Tomography Northern Tibetan Plateau Oil and gas prospect

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北疆文化视野下蒙古马精神的阐释及弘扬

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内蒙古民族大学学报（哲学社会科学版） 2024年第5期50卷 37-44页

作者：王光文高晋内蒙古艺术学院文化艺术管理学院内蒙古呼和浩特010010 内蒙古和林格尔新区管理委员会内蒙古呼和浩特011500

蒙古马精神代表了北疆各族人民的优秀精神,是中华民族精神的组成部分和地方体现,能够为奋力书写中国式现代化新篇章、实现中华民族伟大复兴提供强大的精神动力,因而成为北疆文化的主要精神标识。蒙古马精神的符号意义,来源于蒙古马的自... 详细信息

蒙古马精神代表了北疆各族人民的优秀精神,是中华民族精神的组成部分和地方体现,能够为奋力书写中国式现代化新篇章、实现中华民族伟大复兴提供强大的精神动力,因而成为北疆文化的主要精神标识。蒙古马精神的符号意义,来源于蒙古马的自身品质和历史贡献;蒙古马精神的符号价值,体现为增强中华民族的精神力量。蒙古马精神映照了北疆各族人民吃苦耐劳、开拓进取、忠诚奉献、团结奋斗的历史,契合“踔厉奋发、勇毅前行,为全面建设社会主义现代化国家、全面推进中华民族伟大复兴而团结奋斗”的中国共产党第二十次全国代表大会的主题,传承和弘扬蒙古马精神,具有深厚的历史底蕴和良好的现实基础。在此底蕴和基础上,以彰显北疆文化精神特质、增强中华民族精神力量为目标,从树立和突出各民族共享的中华文化符号、契合中国共产党人精神谱系、加强正确宣传、形成各方合力四个方面,提出阐释和弘扬蒙古马精神的路径。

关键词：北疆文化蒙古马精神中华文化符号

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软土地区复杂环境基坑坍塌原因分析

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地下空间与工程学报 2024年第3期20卷 1039-1050页

作者：黄林伟徐芫蕾周焜王光文浙江省建设工程质量检验站有限公司杭州310012 浙江省建筑科学设计研究院有限公司杭州310012

本文以浙江杭嘉湖冲海积平原地区,紧邻既有建筑、重要管线等复杂环境条件下的某项目基坑为研究对象,基坑开挖影响深度范围内有深厚软土,分别对工程地质条件、坍塌后支护结构现状、支护结构施工质量和技术资料进行了检测调查,并结合支护... 详细信息

本文以浙江杭嘉湖冲海积平原地区,紧邻既有建筑、重要管线等复杂环境条件下的某项目基坑为研究对象,基坑开挖影响深度范围内有深厚软土,分别对工程地质条件、坍塌后支护结构现状、支护结构施工质量和技术资料进行了检测调查,并结合支护结构、构件的计算分析和数值分析,研究表明:该基坑设计时阳角处支护结构选型和布置不合理,土层参数和“m值”取值不合理,基坑局部超挖和基坑支护结构的施工质量缺陷是导致基坑坍塌的原因。本文提出了基坑加固方案和在软土地区复杂环境条件下基坑的设计、施工建议。研究成果可为类似地区和环境条件下基坑的设计、施工提供参考。

关键词：软土地区复杂环境基坑坍塌大阳角受力不平衡

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基于短周期密集台阵接收函数揭示的藏南错那洞穹窿地壳结构

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地学前缘 2024年第1期31卷 170-180页

作者：蔡蔚卢占武黄荣李文辉罗银河王光文穆青程永志陈司王冠陈子龙中国地质科学院地质研究所北京100037 中国地质大学(武汉)地球物理与空间信息学院湖北武汉430074 中国地质科学院自然资源部深地科学与探测技术实验室北京100037 中国地质大学(武汉)地质过程与矿产资源国家重点实验室湖北武汉430074

位于印度-欧亚板块碰撞前缘的北喜马拉雅构造带,是研究陆陆碰撞过程中地壳增厚和深部岩浆活动的典型区域之一。北喜马拉雅穹窿带作为其中重要的伸展构造,其形成过程与造山运动引起的地壳增厚以及地壳深熔作用引起的地壳流动密切相关。... 详细信息

位于印度-欧亚板块碰撞前缘的北喜马拉雅构造带,是研究陆陆碰撞过程中地壳增厚和深部岩浆活动的典型区域之一。北喜马拉雅穹窿带作为其中重要的伸展构造,其形成过程与造山运动引起的地壳增厚以及地壳深熔作用引起的地壳流动密切相关。本文以近年新发现的错那洞穹窿为例,利用短周期密集台阵观测,通过远震P波接收函数共转换叠加成像方法获取了错那洞穹窿区域地壳结构。结果表明:错那洞穹窿区域的地壳结构在东西方向上存在差异,壳内速度间断面存在多处不连续现象,局部区域甚至发生界面错断;而在错那裂谷和错那洞穹窿中、上地壳内存在低速区。综合前人研究,本文认为印度-欧亚板块持续碰撞使喜马拉雅东南缘岩石圈经历过地壳尺度拉伸变形。在地壳增厚及持续的高温变质作用下,中、上地壳随藏南拆离系伸展减压发生部分熔融,而熔融岩浆沿着藏南拆离系薄弱处不断上涌并在错那洞穹窿下方聚集,并使错那洞穹窿及错那裂谷中、上地壳弱化,促进区域东西向伸展,进而发生岩浆底辟形成错那洞穹窿。

关键词：错那洞穹窿短周期密集台阵远震P波接收函数共转换叠加成像地壳结构

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Application of active-source surface waves in urban underground space detection: A case study of Rongcheng County, Hebei, China

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Earth and Planetary Physics 2022年第4期6卷 385-398页

作者： guangwen wang HaiYan wang HongQiang Li ZhanWu Lu WenHui Li TaiRan Xu Lithosphere Center Institute of GeologyChinese Academy of Geological SciencesBeijing 100037China Deep-Earth Dynamics Key Laboratory of the Ministry of Natural Resources Beijing 100037China Chinese Academy of Geological Sciences Beijing 100037China China Earthquake Network Center Beijing 100045China

Active-source surface wave exploration is advantageous because it has high imaging accuracy,is not affected by high-speed layers,and has a low cost;thus,it has unique advantages for investigating shallow surface *** the development and utilization of urban underground space,two parameters in the shallow surface are important,namely,the shear wave velocity(V_(S))and the predominant period of the site,which determine the elevation and aseismic grade of the building *** traditional method is mainly to obtain the two above-mentioned parameters through testing and measuring drilling ***,this method is extremely expensive and time ***,in this research,we used the multichannel surface wave acquisition method to extract the fundamental dispersion curve of single-shot data by using the phase shift method and obtain the V_(S) characteristics in the uppermost 40 m by *** arrived at the following two conclusions based on the V_(S) ***,the study area can be roughly divided into five layers,among which the layers 0−8 m,14−20 m,and 20−30 m are low-velocity layers,corresponding to miscellaneous fill,a water-bearing sand layer,and a sand layer;therefore,the V_(S) is relatively *** contrast,the layers at 8−14 m and 30−40 m are high-velocity layers that are mainly composed of clay,with a relatively better compactness and relatively high V_(S) *** addition,a low-speed anomaly appears abruptly in the high-speed area at 20−40 *** anomaly,when combined with geological data,suggests that it is an ancient river ***,from the V_(S) value,the V_(Se)(equivalent shear wave velocity)was *** construction site soil was categorized as class III,with good conditions for engineering *** addition,we calculated the predominant period of the site to be 0.56-0.77 s based on the V_(S).Therefore,in the overall structural design of the foundation engineering,the natural vibration period of the structure

关键词： Jizhong depression surface wave exploration shallow structure site category predominant period

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Three-dimensional electrical resistivity structure beneath the Cuonadong dome in the Northern Himalayas revealed by magnetotelluric data and its implication

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Science China Earth Sciences 2022年第8期65卷 1538-1553页

作者： Shuai XUE Zhanwu LU Wenhui LI Hongda LIANG guangwen wang Haiyan wang Hongqiang LI Xin LI Key Laboratory of Deep-Earth Dynamics of Ministry of Natural Resources Institute of GeologyChinese Academy of Geological SciencesBeijing100037China Institute of Geophysical and Geochemical Exploration Chinese Academy of Geological SciencesLangfang065000China Chinese Academy of Geological Sciences Beijing100037China State Key Laboratory of Lithospheric Evolution Institute of Geology and GeophysicsChinese Academy of SciencesBeijing100029China

The North Himalayan gneiss domes(NHGD),as one of the extensional structures widely distributed across the southern Tibetan Plateau,are an important window for studying post-collisional diastrophism and magmation as well as polymetallic ***,the deep mechanism for the formation of NHGD remains *** magnetotelluric(MT)method was adopted to study the deep structure of the Cuonadong dome in the Northern *** characteristics of the dome were explored by using the MT sounding curves and phase ***-dimensional(3D)MT inversion was performed to determine the electrical resistivity structure beneath the Cuonadong *** preferred 3D electrical resistivity model shows that an obvious low-resistivity anomaly develops beneath the Cuonadong dome which is overlaid by a high-resistivity body and surrounded by an apparent subcircular zone of low-resistivity *** integrated conductivity(longitudinal conductance)from depths of 1-20 km indicates that the average longitudinal conductance at the core of the Cuonadong dome is about 10,000 *** high-conductivity anomaly at the core is found to be analogous to that of lava,mainly resulting from the crustal partial melting,and the estimated melt content is 11.0-17.3%.The high conductance surrounding the dome reaches 20,000 S on average,which is mainly attributed to saline *** results in this study support that the Cuonadong dome experienced magmatic *** together with previous geological and geochemical studies,we suggest that under the east-west(E-W)extensional tectonic setting in southern Tibet,deep crustal partial melting constantly accumulated beneath the dome,and therefore the magmatic diapirism resulted in the formation of the Cuonadong *** addition,the MT results also indicate that the development of the Cuonadong dome provides abundant mineralizing fluids and the space for migration of metallogenic fluids for(rare-metal)polymetallic mineralization.

关键词： Cuonadong dome Magnetotelluric method North Himalayan gneiss domes Magmatic diapirism Partial melting Extension

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发泡水泥板的应用背景、现状及前景概述

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材料导报 2024年第14期38卷 113-121页

作者：雷东移刘承侃刘佳鑫丛波王光文张鹏李莹董玉玲李智鸿刘加平青岛理工大学土木工程学院山东青岛266033 海洋环境混凝土技术教育部工程研究中心山东青岛266520 辽宁超强防火保温科技有限公司辽宁丹东118002 广东东鹏控股股份有限公司广东佛山528031 东南大学材料科学与工程学院南京211189

为深层次、全面了解发泡水泥板诞生背景、应用现状和发展前景,本文对发泡水泥板应用背景、市场需求、技术标准沿变、整体性能、综合成本、应用现状、发展前景进行全方位、多角度、深层次剖析。综合认为:尽管发泡水泥板具有隔热、防火(A1... 详细信息

为深层次、全面了解发泡水泥板诞生背景、应用现状和发展前景,本文对发泡水泥板应用背景、市场需求、技术标准沿变、整体性能、综合成本、应用现状、发展前景进行全方位、多角度、深层次剖析。综合认为:尽管发泡水泥板具有隔热、防火(A1级)、环保、抗老化、强度高等优点,但其研发技术壁垒低,加上劣质产品挡道、厂家恶性竞争等恶劣现象,致使其市场容纳空间已非常狭小。本文将为建筑从业人员全面了解发泡水泥板的“来龙去脉”和实时动态提供最新现实科学依据,为科研工作者研发新型建筑保温材料提供更多理论科学依据。

关键词：发泡水泥板泡沫混凝土保温外墙防火

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黄牛源产气荚膜梭菌分离株基因组的生物信息学分析

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畜牧兽医学报 2024年第4期55卷 1707-1715页

作者：田睿徐思翔谢烽刘广锦王刚李庆霞代蕾谢国信张琼文陆亚警王光文王金秀张炜南京农业大学三亚研究院三亚572025 南京农业大学动物医学院南京210095 南京农业大学WOAH猪链球菌参考实验室南京210095 海南省动物疫病预防控制中心海口571100 昌江黎族自治县畜牧兽医技术服务中心昌江572799

本研究自猝死海南黄牛的组织器官中分离到2株A型产气荚膜梭菌,命名为ZWCP209和ZWCP210。基因组拼接结果显示其基因组大小处于3.4~3.5 Mb之间,tRNA和CDS数量稳定。多位点序列分析(MLST)显示,测序菌株属于同种新ST型。从NCBI数据库中获取2... 详细信息

本研究自猝死海南黄牛的组织器官中分离到2株A型产气荚膜梭菌,命名为ZWCP209和ZWCP210。基因组拼接结果显示其基因组大小处于3.4~3.5 Mb之间,tRNA和CDS数量稳定。多位点序列分析(MLST)显示,测序菌株属于同种新ST型。从NCBI数据库中获取27株牛源产气荚膜梭菌的基因组,与测序分离株共同进行生物信息学分析:共检测到13种耐药基因,其中四环素类耐药基因tetA(P)和tetB(P)的携带率最高(79.4%),值得关注的是,本研究中2株海南黄牛分离株均携带了7种以上的耐药基因,其中包括非兽用抗生素噁唑烷酮的耐药基因optrA。泛基因组分析结果显示以上菌株的基因组中共含有7 345个基因,核心基因数量占比22.94%;在核心基因组和plc基因的系统进化分析中,两海南黄牛分离株处于同一分支,并具有相同的毒力因子,具有极高的同源性。本研究为国内首次对海南黄牛产气荚膜梭菌进行全基因组序列测定与生物信息学分析,对牛产气荚膜梭菌病的防治与基因组研究具有参考价值。

关键词：海南黄牛产气荚膜梭菌分离鉴定全基因组测序生物信息学分析

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Influenza A virus use of BinCARD1 to facilitate the binding of viral NP to importinα7 is counteracted by TBK1-p62 axis-mediated autophagy

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Cellular & Molecular Immunology 2022年第10期19卷 1168-1184页

作者： Xuyuan wang Li Jiang guangwen wang Wenjun Shi Yuzhen Hu Bo wang Xianying Zeng Guobin Tian Guohua Deng Jianzhong Shi Liling Liu Chengjun Li Hualan Chen College of Veterinary Medicine Gansu Agricultural UniversityLanzhou730070China Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou510642China State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin150069China

As a major component of the viral ribonucleoprotein(vRNP)complex in influenza A virus(IAV),nucleoprotein(NP)interacts with isoforms of importinαfamily members,leading to the import of itself and vRNP complex into the nucleus,a process pivotal in the replication cycle of *** this study,we found that BinCARD1,an isoform of Bcl10-interacting protein with CARD(BinCARD),was leveraged by IAV for efficient viral ***1 promoted the nuclear import of the vRNP complex and newly synthesized NP and thus enhanced vRNP complex ***,we found that BinCARD1 interacted with NP to promote NP binding to importinα7,an adaptor in the host nuclear import ***,we also found that BinCARD1 promoted RIG-I-mediated innate immune signaling by mediating Lys63-linked polyubiquitination of TRAF3,and that TBK1 appeared to degrade *** showed that BinCARD1 was polyubiquitinated at residue K103 through a Lys63 linkage,which was recognized by the TBK1-p62 axis for autophagic ***,our data demonstrate that IAV leverages BinCARD1 as an important host factor that promotes viral replication,and two mechanisms in the host defense system are triggered—innate immune signaling and autophagic degradation—to mitigate the promoting effect of BinCARD1 on the life cycle of IAV.

关键词： Influenza A virus BinCARD1 TRAF3 TBK1 p62

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